P19729 (DMPK_PSEUF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenol hydroxylase P0 protein
EC=1.14.13.7

Alternative name(s):
Phenol 2-monooxygenase P0 component

Gene names

Name:	dmpK
Synonyms:	pheA1

Encoded on

Plasmid pVI150

Organism

Pseudomonas sp. (strain CF600)

Taxonomic identifier

79676 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Catabolizes phenol, and some of its methylated derivatives. P0 is required for growth on phenol, but not for in vitro phenol hydroxylase activity.
Catalytic activity	Phenol + NADPH + O2 = catechol + NADP+ + H2O.
Cofactor	FAD. Fe2+.
Pathway	Aromatic compound metabolism; phenol degradation.
Subunit structure	The multicomponent enzyme phenol hydroxylase is formed by P0, P1, P2, P3, P4 and P5 polypeptides.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	FAD Flavoprotein Iron NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phenol 2-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 92	92	Phenol hydroxylase P0 protein		PRO_0000079940

Sequences

	Sequence		Length	Mass (Da)
	P19729 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 9893DFD3CAACB71E	FASTA	92	10,586
	10 20 30 40 50 60 MTVTNTPTPT FDQLTRYIRV RSEPEAKFVE FDFAIGHPEL FVELVLPQDA FVKFCQHNRV 70 80 90 VAMDEAMAKA VDDDMVKWRF GDVGRRLPKD PG

References

[1]	"Complete nucleotide sequence and polypeptide analysis of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600." Nordlund I., Powlowski J., Shingler V. J. Bacteriol. 172:6826-6833(1990) [PubMed: 2254258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Takeo M., Maeda Y., Okada H., Miyama K., Mori K., Ike M., Fujita M. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BH.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60276 Genomic DNA. Translation: AAA25939.1. D28864 Genomic DNA. Translation: BAA06014.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12794.
Family and domain databases
InterPro	IPR010353. Phenol_hyd_sub. [Graphical view]
Pfam	PF06099. Phenol_hyd_sub. 1 hit. [Graphical view]
PIRSF	PIRSF000039. Phenol_monooxy_K. 1 hit.
ProDom	PD468738. Phenol_hyd_sub. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

DMPK_PSEUF

Accession

Primary (citable) accession number: P19729

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	July 27, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program