P19721 (SPIKE_ADEM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fiber protein
Short name=SPIKE

Alternative name(s):
Protein IV

Gene names

ORF Names:

L5

Organism

Murine adenovirus A serotype 1 (MAdV-1) (Murine adenovirus 1)

Taxonomic identifier

10530 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus › Murine adenovirus A

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host receptor to provide virion initial attachment to target cell. Fiber proteins are shed during virus entry, when virus is still at the cell surface By similarity.
Subunit structure	Homotrimer. Interacts (via N-terminal tail region) with pentons By similarity.
Subcellular location	Virion By similarity. Host nucleus By similarity. Note: Anchored to the pentons, protrudes from the virion surface By similarity.
Induction	Expressed in the late phase of the viral replicative cycle.
Domain	The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion By similarity.
Miscellaneous	All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell By similarity.
Sequence similarities	Belongs to the adenoviridae fiber family.

Ontologies

Keywords
Biological process	Host-virus interaction Viral attachment to host cell Virus entry into host cell
Cellular component	Host nucleus Virion
Developmental stage	Late protein
Molecular function	Capsid protein
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: InterPro viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Fiber protein		PRO_0000221810

Sequences

	Sequence		Length	Mass (Da)
	P19721 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 4C6E7936DD594247	FASTA	613	66,802
	10 20 30 40 50 60 MVEALNAVYP YDLALLPEDY EKTTAPDAVQ AANAARPFLN PVYPYQQPVA GDFGFPIVMP 70 80 90 100 110 120 PFFNSYDFTS IHGNTLSLRL NKPLKRTAKG LQLLLGSGLS VNADGQLESS EGISEADAPL 130 140 150 160 170 180 QINDGVLQLS FGEGLSVNDH GELESKGKVE AVTLPLALQD HVMSLSFGQG LQVNDQGQLE 190 200 210 220 230 240 ALAMVHSTSA PLKVTNNNLE LALGRGLIVD DQGQLRLAPN LLWPESPLAI EQGTNHLILF 250 260 270 280 290 300 YNQSLDVEDG KLTLPEPFDP LTLDGGRLRM QLAPNSGLAV TEKGSLGINW GEGIQVKEQK 310 320 330 340 350 360 ITLKVTPANG LAVTEQGGLN INWGNGIKVD EQKVTLKTSN EFALTENGLY LTSPLNPIEV 370 380 390 400 410 420 NQHGQLGIAL GYGFHAHRGY LELTPQTLWT GLPIGNNGTF HTKQDCKIFL SLTRLGPMVH 430 440 450 460 470 480 GTFMLQAPQY ELTTNGMREI TFSFNSTGGL EQPAPVTYWG ALDPPPTAKA AEIENQKRVK 490 500 510 520 530 540 KRAAPDPPVE PPPKRRGDLA VLFAKVAEQA MELAKEQAVQ AQPPEHVNTD WADHMNLLRF 550 560 570 580 590 600 MPNTLVYPTA ATIAANLQFH DTRLSLRRAT LKIRLNGSPD SAYQLGFMLE LVGTQSASIV 610 TDTISFWYYA EDY

References

[1]	"The mouse adenovirus type 1 contains an unusual E3 region." Raviprakash K.S., Grunhaus A., el Kholy M.A., Horwitz M.S. J. Virol. 63:5455-5458(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M30594 Genomic DNA. Translation: AAA42434.1.
PIR	ERADFM. C33382.
3D structure databases
ProteinModelPortal	P19721.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
ProtClustDB	CLSP2509584.
Family and domain databases
Gene3D	2.10.25.20. 3 hits. 2.60.90.10. 2 hits.
InterPro	IPR000931. Adeno_fibre. IPR000978. Adeno_fibre_knob. IPR000939. Adenobir_fibre_prot_rpt/shaft. IPR008982. Adenovirus_pIV-rel_att. IPR009013. Attachment_protein_shaft_dom. [Graphical view]
Pfam	PF00541. Adeno_knob. 1 hit. PF00608. Adeno_shaft. 1 hit. [Graphical view]
PRINTS	PR00307. ADENOVSFIBRE.
SUPFAM	SSF49835. Viral_att. 1 hit. SSF51225. Viral_att_shaft. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SPIKE_ADEM1

Accession

Primary (citable) accession number: P19721

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program