Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myosin heavy chain IB

Gene

MIB

Organism
Acanthamoeba castellanii (Amoeba)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi103 – 1108ATPSequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin heavy chain IB
Alternative name(s):
Myosin heavy chain IL
Gene namesi
Name:MIB
Synonyms:MIL
OrganismiAcanthamoeba castellanii (Amoeba)
Taxonomic identifieri5755 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaDiscoseaLongamoebiaCentramoebidaAcanthamoebidaeAcanthamoeba

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11471147Myosin heavy chain IBPRO_0000123361Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei315 – 3151Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP19706.

Interactioni

Subunit structurei

Myosin I heavy chain is single-headed. Dimer of a heavy and a light chain. Inability to self-assemble into filaments.

Structurei

Secondary structure

1
1147
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1094 – 10996Combined sources
Beta strandi1116 – 11216Combined sources
Beta strandi1126 – 11327Combined sources
Beta strandi1135 – 11406Combined sources
Helixi1141 – 11433Combined sources
Beta strandi1144 – 11463Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DRKX-ray1.42A1094-1147[»]
2DRMX-ray1.35A/B/C/D1094-1147[»]
ProteinModelPortaliP19706.
SMRiP19706. Positions 1094-1147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19706.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 677669Myosin motorAdd
BLAST
Domaini715 – 900186TH1PROSITE-ProRule annotationAdd
BLAST
Domaini1090 – 114758SH3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi908 – 1089182Ala/Gly/Pro-rich (TH.2)Add
BLAST

Domaini

Myosin tail domain binds directly to anionic phospholipid membranes; myosins I could therefore move actin relative to membranes and vice versa. TH.2 and SH3 bind tightly to F-actin; this together with the nucleotide-sensitive site in the head, allows single molecules of myosin I to cross-link actin filaments.

Sequence similaritiesi

Contains 1 myosin motor domain.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 TH1 (class I myosin tail homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Family and domain databases

InterProiIPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
PR00452. SH3DOMAIN.
SMARTiSM00242. MYSc. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
PS50002. SH3. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19706-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKAAVEQRG VDDLVLMPKI TEQDICANLE KRYFNDLIYT NIGPVLISVN
60 70 80 90 100
PFRRIDALLT DECLHCYRGR YQHEQPPHVY ALAEAAYRGV KSENINQCVI
110 120 130 140 150
ISGESGAGKT EASKLVMQYV AAVSGNSGGV DFVKHSNPLL EAFGNAKTLR
160 170 180 190 200
NNNSSRFGKY FEIHFNRLGE PCGGRITNYL LEKSRVTFQT RGERSFHIFY
210 220 230 240 250
QLLAGASDAE AQEMQLYAPE NFNYLNQSAC YTVDGIDDIK EFADTRNAIN
260 270 280 290 300
VMGMTAEEQR QVFHLVAGIL HLGNVAFHDG GKGTAAVHDR TPFALKNALL
310 320 330 340 350
FRVLNTGGAG AKKMSTYNVP QNVEQAASAR DALAKTIYSR MFDWIVSKVN
360 370 380 390 400
EALQKQGGSG DHNNNMIGVL DIFGFEIFEQ NGFEQFCINY VNEKLQQYFI
410 420 430 440 450
ELTLKAEQEE YVNEGIQWTP IKYFNNKVVC ELIEGKRPPG IFSLLDDICF
460 470 480 490 500
TMHAQSDGMD GKFLQKCQGG FPSHLHFRGM NNAFSIKHYA GEVTYEAEGF
510 520 530 540 550
CEKNKDTLFD DLIAVIQESE NRLLVSWFPE DTKQLQKKRP TTAGFKLKTS
560 570 580 590 600
CDALMEALSR CSPHYIRCIK PNDNKAYHDW DATRTKHQVQ YLGLLENVRV
610 620 630 640 650
RRAGFAYRAE FDRFLRRYKK LSPKTWGIWG EWSGAPKDGC QTLLNDLGLD
660 670 680 690 700
TSQWQLGKSK VFIRYPETLF HLEECLDRKD YDCTLRIQKA WRHWKSRKHQ
710 720 730 740 750
LEQRKMAADL LKGKKERQRH SVNRKYEFDY INYDANYPLQ DCVRSSGRDK
760 770 780 790 800
EATAFTDQVL VLNRRGKPER RDLIVTNEAV YFAMRKKKSG QVVYNLKRRI
810 820 830 840 850
PLGEIASLSL STLQDNYVVI HHNQYDMVFE NDKKTEIVTI LMENYKMSGG
860 870 880 890 900
RDLPVNFNDN ITYKASNGAQ RRLTFSKNES ASAQPSIKKS RANIQIGIAT
910 920 930 940 950
GLPKETDSSP PNWTPSGGGG GYGGGRGGGG GGRGAAGGGR GGFGGGGGGG
960 970 980 990 1000
YSQPVAQAQP VAQVPQPVAA VPSAGRGGPG MGGPGAGRGG PGMGRGGPGM
1010 1020 1030 1040 1050
GGPGAGRGGP GMGGPGGPGR GGPGGPGAGR GGPGGPGAGR GGPGMGGPGG
1060 1070 1080 1090 1100
AGRGGPGAGR GGPGMGGPGA GRGGPGAGRG AAPAPAPAAP AKPQVKALYD
1110 1120 1130 1140
YDAQTGDELT FKEGDTIIVH QKDPAGWWEG ELNGKRGWVP ANYVQDI
Length:1,147
Mass (Da):124,959
Last modified:November 1, 1995 - v2
Checksum:iB76DE9C076381054
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30780 Genomic DNA. Translation: AAA27708.1.
PIRiJQ0095. MWAXIB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30780 Genomic DNA. Translation: AAA27708.1.
PIRiJQ0095. MWAXIB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DRKX-ray1.42A1094-1147[»]
2DRMX-ray1.35A/B/C/D1094-1147[»]
ProteinModelPortaliP19706.
SMRiP19706. Positions 1094-1147.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP19706.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP19706.

Family and domain databases

InterProiIPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
PR00452. SH3DOMAIN.
SMARTiSM00242. MYSc. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51456. MYOSIN_MOTOR. 1 hit.
PS50002. SH3. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Myosin I heavy-chain genes of Acanthamoeba castellanii: cloning of a second gene and evidence for the existence of a third isoform."
    Jung G., Schmidt C.J., Hammer J.A. III
    Gene 82:269-280(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The localization and sequence of the phosphorylation sites of Acanthamoeba myosins I. An improved method for locating the phosphorylated amino acid."
    Brzeska H., Lynch T.J., Martin B., Korn E.D.
    J. Biol. Chem. 264:19340-19348(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 538-559, PHOSPHORYLATION AT SER-315.

Entry informationi

Entry nameiMYSB_ACACA
AccessioniPrimary (citable) accession number: P19706
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 1, 1995
Last modified: January 20, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

This organism expresses at least three isoforms of myosin I heavy-chain, encoded by genes MIA, MIB, and MIC.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.