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Protein

Myosin heavy chain IB

Gene

MIB

Organism
Acanthamoeba castellanii (Amoeba)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Myosin is a protein that binds to F-actin and has ATPase activity that is activated by F-actin.

Miscellaneous

This organism expresses at least three isoforms of myosin I heavy-chain, encoded by genes MIA, MIB, and MIC.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi103 – 110ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Motor protein, Myosin
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin heavy chain IB
Alternative name(s):
Myosin heavy chain IL
Gene namesi
Name:MIB
Synonyms:MIL
OrganismiAcanthamoeba castellanii (Amoeba)
Taxonomic identifieri5755 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaDiscoseaLongamoebiaCentramoebidaAcanthamoebidaeAcanthamoeba

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001233611 – 1147Myosin heavy chain IBAdd BLAST1147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei315Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP19706

Interactioni

Subunit structurei

Myosin I heavy chain is single-headed. Dimer of a heavy and a light chain. Inability to self-assemble into filaments.

GO - Molecular functioni

Structurei

Secondary structure

11147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1094 – 1099Combined sources6
Beta strandi1116 – 1121Combined sources6
Beta strandi1126 – 1132Combined sources7
Beta strandi1135 – 1140Combined sources6
Helixi1141 – 1143Combined sources3
Beta strandi1144 – 1146Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DRKX-ray1.42A1094-1147[»]
2DRMX-ray1.35A/B/C/D1094-1147[»]
ProteinModelPortaliP19706
SMRiP19706
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19706

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 677Myosin motorPROSITE-ProRule annotationAdd BLAST669
Domaini715 – 900TH1PROSITE-ProRule annotationAdd BLAST186
Domaini1090 – 1147SH3PROSITE-ProRule annotationAdd BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni551 – 573Actin-bindingPROSITE-ProRule annotationAdd BLAST23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi908 – 1089Ala/Gly/Pro-rich (TH.2)Add BLAST182

Domaini

Myosin tail domain binds directly to anionic phospholipid membranes; myosins I could therefore move actin relative to membranes and vice versa. TH.2 and SH3 bind tightly to F-actin; this together with the nucleotide-sensitive site in the head, allows single molecules of myosin I to cross-link actin filaments.

Sequence similaritiesi

Keywords - Domaini

SH3 domain

Family and domain databases

CDDicd01378 MYSc_Myo1, 1 hit
Gene3Di3.40.850.10, 3 hits
InterProiView protein in InterPro
IPR036961 Kinesin_motor_dom_sf
IPR001609 Myosin_head_motor_dom
IPR010926 Myosin_TH1
IPR036072 MYSc_Myo1
IPR027417 P-loop_NTPase
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00063 Myosin_head, 1 hit
PF06017 Myosin_TH1, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00193 MYOSINHEAVY
PR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00242 MYSc, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51456 MYOSIN_MOTOR, 1 hit
PS50002 SH3, 1 hit
PS51757 TH1, 1 hit

Sequencei

Sequence statusi: Complete.

P19706-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKAAVEQRG VDDLVLMPKI TEQDICANLE KRYFNDLIYT NIGPVLISVN
60 70 80 90 100
PFRRIDALLT DECLHCYRGR YQHEQPPHVY ALAEAAYRGV KSENINQCVI
110 120 130 140 150
ISGESGAGKT EASKLVMQYV AAVSGNSGGV DFVKHSNPLL EAFGNAKTLR
160 170 180 190 200
NNNSSRFGKY FEIHFNRLGE PCGGRITNYL LEKSRVTFQT RGERSFHIFY
210 220 230 240 250
QLLAGASDAE AQEMQLYAPE NFNYLNQSAC YTVDGIDDIK EFADTRNAIN
260 270 280 290 300
VMGMTAEEQR QVFHLVAGIL HLGNVAFHDG GKGTAAVHDR TPFALKNALL
310 320 330 340 350
FRVLNTGGAG AKKMSTYNVP QNVEQAASAR DALAKTIYSR MFDWIVSKVN
360 370 380 390 400
EALQKQGGSG DHNNNMIGVL DIFGFEIFEQ NGFEQFCINY VNEKLQQYFI
410 420 430 440 450
ELTLKAEQEE YVNEGIQWTP IKYFNNKVVC ELIEGKRPPG IFSLLDDICF
460 470 480 490 500
TMHAQSDGMD GKFLQKCQGG FPSHLHFRGM NNAFSIKHYA GEVTYEAEGF
510 520 530 540 550
CEKNKDTLFD DLIAVIQESE NRLLVSWFPE DTKQLQKKRP TTAGFKLKTS
560 570 580 590 600
CDALMEALSR CSPHYIRCIK PNDNKAYHDW DATRTKHQVQ YLGLLENVRV
610 620 630 640 650
RRAGFAYRAE FDRFLRRYKK LSPKTWGIWG EWSGAPKDGC QTLLNDLGLD
660 670 680 690 700
TSQWQLGKSK VFIRYPETLF HLEECLDRKD YDCTLRIQKA WRHWKSRKHQ
710 720 730 740 750
LEQRKMAADL LKGKKERQRH SVNRKYEFDY INYDANYPLQ DCVRSSGRDK
760 770 780 790 800
EATAFTDQVL VLNRRGKPER RDLIVTNEAV YFAMRKKKSG QVVYNLKRRI
810 820 830 840 850
PLGEIASLSL STLQDNYVVI HHNQYDMVFE NDKKTEIVTI LMENYKMSGG
860 870 880 890 900
RDLPVNFNDN ITYKASNGAQ RRLTFSKNES ASAQPSIKKS RANIQIGIAT
910 920 930 940 950
GLPKETDSSP PNWTPSGGGG GYGGGRGGGG GGRGAAGGGR GGFGGGGGGG
960 970 980 990 1000
YSQPVAQAQP VAQVPQPVAA VPSAGRGGPG MGGPGAGRGG PGMGRGGPGM
1010 1020 1030 1040 1050
GGPGAGRGGP GMGGPGGPGR GGPGGPGAGR GGPGGPGAGR GGPGMGGPGG
1060 1070 1080 1090 1100
AGRGGPGAGR GGPGMGGPGA GRGGPGAGRG AAPAPAPAAP AKPQVKALYD
1110 1120 1130 1140
YDAQTGDELT FKEGDTIIVH QKDPAGWWEG ELNGKRGWVP ANYVQDI
Length:1,147
Mass (Da):124,959
Last modified:November 1, 1995 - v2
Checksum:iB76DE9C076381054
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30780 Genomic DNA Translation: AAA27708.1
PIRiJQ0095 MWAXIB

Similar proteinsi

Entry informationi

Entry nameiMYSB_ACACA
AccessioniPrimary (citable) accession number: P19706
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing
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Main funding by: National Institutes of Health