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P19687

- GCYA1_BOVIN

UniProt

P19687 - GCYA1_BOVIN

Protein

Guanylate cyclase soluble subunit alpha-1

Gene

GUCY1A1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    GTP = 3',5'-cyclic GMP + diphosphate.

    Enzyme regulationi

    Activated by nitric oxide in the presence of magnesium or manganese ions.

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. guanylate cyclase activity Source: UniProtKB-EC
    3. heme binding Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. positive regulation of cGMP biosynthetic process Source: Ensembl
    3. regulation of blood pressure Source: Ensembl
    4. relaxation of vascular smooth muscle Source: Ensembl
    5. response to defense-related host nitric oxide production Source: Ensembl

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    cGMP biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_208097. Nitric oxide stimulates guanylate cyclase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanylate cyclase soluble subunit alpha-1 (EC:4.6.1.2)
    Short name:
    GCS-alpha-1
    Alternative name(s):
    Soluble guanylate cyclase large subunit
    Gene namesi
    Name:GUCY1A1
    Synonyms:GUC1A1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 17

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 691691Guanylate cyclase soluble subunit alpha-1PRO_0000074108Add
    BLAST

    Proteomic databases

    PRIDEiP19687.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta chain.

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000019398.

    Structurei

    Secondary structure

    1
    691
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi484 – 4874
    Helixi490 – 4945
    Beta strandi497 – 4993
    Helixi502 – 51514
    Beta strandi521 – 5244
    Beta strandi535 – 5373
    Beta strandi540 – 5434
    Helixi545 – 56521
    Turni566 – 5683
    Beta strandi575 – 5784
    Beta strandi584 – 5885
    Beta strandi590 – 5923
    Beta strandi594 – 5996
    Helixi600 – 61112
    Helixi622 – 6276

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AWNmodel-A472-628[»]
    ProteinModelPortaliP19687.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini482 – 609128Guanylate cyclasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
    Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2114.
    GeneTreeiENSGT00730000110520.
    HOGENOMiHOG000220903.
    HOVERGENiHBG106603.
    InParanoidiP19687.
    KOiK12318.
    OMAiSEIPGIC.
    OrthoDBiEOG7VX8Z6.
    TreeFamiTF351403.

    Family and domain databases

    Gene3Di3.30.70.1230. 1 hit.
    InterProiIPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR011645. Haem_no_assoc-bd.
    IPR011644. Heme_NO-bd.
    IPR024096. NO_sig/Golgi_transp_ligand-bd.
    [Graphical view]
    PfamiPF00211. Guanylate_cyc. 1 hit.
    PF07700. HNOB. 1 hit.
    PF07701. HNOBA. 1 hit.
    [Graphical view]
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111126. SSF111126. 1 hit.
    SSF55073. SSF55073. 1 hit.
    PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19687-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFCAKLKDLQ ITGDCPFSLL APGQVPREPL GEATGSGPAS TPGQPGVCPG    50
    VPDKNPPGRL PRRKTSRSRV YLHTLAESIC KLIFPEFERL NLALQRTLAK 100
    HKIKENRKSL EREDFEKIVV DQAIAAGVPV EIIKESLGEE LFKICYEEDE 150
    YILGVVGGTL KDFLNSFSTL LKQSSHCQEA EKKGRFEDAS ILCLDKDPDV 200
    LYVYYFFPKR ITSLILPGII KAAARILYET EVEVSSTPSR FHQDCREFVD 250
    QPCELYSVHI RSARPHPPPG KPVSSLVIPA SLFCKTFPFH FMLDRDMSIL 300
    QLGHGIRRLM SRRDVQGKPH FDEYFEILTP KISQTFSGIM TMLNMQFLVR 350
    VRRWDNSMKK SSRVMDLKGQ MIYMVESSSI LFLGSPCVDR LEDFTGRGLY 400
    LSDIPIHNAL RDVVLIGEQA RAQDGLKKRL GKLKATLEQA HQALEEEKRK 450
    TVDLLCSIFP SEVARQLWQG HAVQAKRFGN VTMLFSDIVG FTAICSQCSP 500
    LQVITMLNAL YTRFDRQCGE LDVYKVETIG DAYCVAGGLH KESDTHAVQI 550
    ALMALKMMEL SHEVVSPHGE PIKMRIGLHS GSVFAGVVGV KMPRYCLFGN 600
    NVTLANKFES CSVPRKINVS PTTYRLLKDC PGFVFTPRSR EELPPNFPSD 650
    IPGICHFLEA YQQGTTSKPW FQKKDVEEAN ANFLGKASGI D 691
    Length:691
    Mass (Da):77,533
    Last modified:February 1, 1991 - v1
    Checksum:i5D1FE4D2204E8683
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54014 mRNA. Translation: CAA37960.1.
    PIRiS10713. OYBO77.
    RefSeqiNP_786972.1. NM_175778.2.
    XP_005217696.1. XM_005217639.1.
    XP_005217697.1. XM_005217640.1.
    XP_005217698.1. XM_005217641.1.
    XP_005217699.1. XM_005217642.1.
    XP_005217700.1. XM_005217643.1.
    XP_005217701.1. XM_005217644.1.
    UniGeneiBt.4480.

    Genome annotation databases

    EnsembliENSBTAT00000019398; ENSBTAP00000019398; ENSBTAG00000014576.
    GeneIDi281216.
    KEGGibta:281216.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54014 mRNA. Translation: CAA37960.1 .
    PIRi S10713. OYBO77.
    RefSeqi NP_786972.1. NM_175778.2.
    XP_005217696.1. XM_005217639.1.
    XP_005217697.1. XM_005217640.1.
    XP_005217698.1. XM_005217641.1.
    XP_005217699.1. XM_005217642.1.
    XP_005217700.1. XM_005217643.1.
    XP_005217701.1. XM_005217644.1.
    UniGenei Bt.4480.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AWN model - A 472-628 [» ]
    ProteinModelPortali P19687.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000019398.

    Chemistry

    BindingDBi P19687.

    Proteomic databases

    PRIDEi P19687.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000019398 ; ENSBTAP00000019398 ; ENSBTAG00000014576 .
    GeneIDi 281216.
    KEGGi bta:281216.

    Organism-specific databases

    CTDi 2982.

    Phylogenomic databases

    eggNOGi COG2114.
    GeneTreei ENSGT00730000110520.
    HOGENOMi HOG000220903.
    HOVERGENi HBG106603.
    InParanoidi P19687.
    KOi K12318.
    OMAi SEIPGIC.
    OrthoDBi EOG7VX8Z6.
    TreeFami TF351403.

    Enzyme and pathway databases

    Reactomei REACT_208097. Nitric oxide stimulates guanylate cyclase.

    Miscellaneous databases

    NextBioi 20805268.

    Family and domain databases

    Gene3Di 3.30.70.1230. 1 hit.
    InterProi IPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR011645. Haem_no_assoc-bd.
    IPR011644. Heme_NO-bd.
    IPR024096. NO_sig/Golgi_transp_ligand-bd.
    [Graphical view ]
    Pfami PF00211. Guanylate_cyc. 1 hit.
    PF07700. HNOB. 1 hit.
    PF07701. HNOBA. 1 hit.
    [Graphical view ]
    SMARTi SM00044. CYCc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111126. SSF111126. 1 hit.
    SSF55073. SSF55073. 1 hit.
    PROSITEi PS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The primary structure of the larger subunit of soluble guanylyl cyclase from bovine lung. Homology between the two subunits of the enzyme."
      Koesling D., Harteneck C., Humbert P., Bosserhoff A., Frank R., Schultz G., Boehme E.
      FEBS Lett. 266:128-132(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Adrenal medulla.
    2. "Catalytic mechanism of the adenylyl and guanylyl cyclases: modeling and mutational analysis."
      Liu Y., Ruoho A.E., Rao V.D., Hurley J.H.
      Proc. Natl. Acad. Sci. U.S.A. 94:13414-13419(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 472-628.

    Entry informationi

    Entry nameiGCYA1_BOVIN
    AccessioniPrimary (citable) accession number: P19687
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3