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Protein

Guanylate cyclase soluble subunit alpha-3

Gene

Gucy1a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.By similarity

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Has also activity with Mn2+ (in vitro).By similarity

Enzyme regulationi

Activated by nitric oxide in the presence of magnesium or manganese ions.By similarity

GO - Molecular functioni

  • GTP binding Source: RGD
  • guanylate cyclase activity Source: RGD
  • ion binding Source: RGD
  • protein heterodimerization activity Source: RGD

GO - Biological processi

  • cAMP biosynthetic process Source: GOC
  • cGMP biosynthetic process Source: RGD
  • nitric oxide mediated signal transduction Source: RGD
  • response to herbicide Source: RGD
  • response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cGMP biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.2. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase soluble subunit alpha-3 (EC:4.6.1.2By similarity)
Short name:
GCS-alpha-3
Alternative name(s):
GCS-alpha-1
Soluble guanylate cyclase large subunit
Gene namesi
Name:Gucy1a3
Synonyms:Guc1a1, Gucy1a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68436. Gucy1a3.

Subcellular locationi

GO - Cellular componenti

  • guanylate cyclase complex, soluble Source: RGD
  • plasma membrane Source: GO_Central
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741121 – 690Guanylate cyclase soluble subunit alpha-3Add BLAST690

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei266PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP19686.
PRIDEiP19686.

Interactioni

Subunit structurei

The active enzyme is formed by a heterodimer of an alpha and a beta subunit. Heterodimer with GUCY1B3.By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP19686. 2 interactors.
STRINGi10116.ENSRNOP00000017190.

Structurei

3D structure databases

ProteinModelPortaliP19686.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini480 – 607Guanylate cyclasePROSITE-ProRule annotationAdd BLAST128

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4171. Eukaryota.
COG2114. LUCA.
HOVERGENiHBG106603.
InParanoidiP19686.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011645. HNOB_dom_associated.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19686-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFCRKFKDLK ITGECPFSLL APGQVPTEPI EEVAGVSESC QATLPTCQEF
60 70 80 90 100
AENAEGSHPQ RKTSRNRVYL HTLAESIGKL IFPEFERLNL ALQRTLAKHK
110 120 130 140 150
IKENRNSSEK EDLERIIAEE AIAAGVPVEV LKDSLGEELF KICYEEDEHI
160 170 180 190 200
LGVVGGTLKD FLNSFSTLLK QSSHCQEAER RGRLEDASIL CLDKDQDFLN
210 220 230 240 250
VYYFFPKRTT ALLLPGIIKA AARILYESHV EVSLMPPCFR SECTEFVNQP
260 270 280 290 300
YLLYSVHVKS TKPSLSPGKP QSSLVIPTSL FCKTFPFHFM LDRDLAILQL
310 320 330 340 350
GNGIRRLVNK RDFQGKPNFE EFFEILTPKI NQTFSGIMTM LNMQFVIRVR
360 370 380 390 400
RWDNLVKKSS RVMDLKGQMI YIVESSAILF LGSPCVDRLE DFTGRGLYLS
410 420 430 440 450
DIPIHNALRD VVLIGEQARA QDGLKKRLGK LKATLEHAHQ ALEEEKKKTV
460 470 480 490 500
DLLCSIFPSE VAQQLWQGQI VQAKKFNEVT MLFSDIVGFT AICSQCSPLQ
510 520 530 540 550
VITMLNALYT RFDQQCGELD VYKVETIGDA YCVAGGLHRE SDTHAVQIAL
560 570 580 590 600
MALKMMELSN EVMSPHGEPI KMRIGLHSGS VFAGVVGVKM PRYCLFGNNV
610 620 630 640 650
TLANKFESCS VPRKINVSPT TYRLLKDCPG FVFTPRSREE LPPNFPSDIP
660 670 680 690
GICHFLDAYQ HQGPNSKPWF QQKDAEDGNA NFLGKASGVD
Length:690
Mass (Da):77,567
Last modified:February 1, 1991 - v1
Checksum:iE4819B2CA4F86401
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57405 mRNA. Translation: AAA41206.1.
U60835 mRNA. Translation: AAB17953.1.
PIRiA38297. OYRTA1.
UniGeneiRn.1974.

Genome annotation databases

UCSCiRGD:68436. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57405 mRNA. Translation: AAA41206.1.
U60835 mRNA. Translation: AAB17953.1.
PIRiA38297. OYRTA1.
UniGeneiRn.1974.

3D structure databases

ProteinModelPortaliP19686.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19686. 2 interactors.
STRINGi10116.ENSRNOP00000017190.

Proteomic databases

PaxDbiP19686.
PRIDEiP19686.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:68436. rat.

Organism-specific databases

RGDi68436. Gucy1a3.

Phylogenomic databases

eggNOGiKOG4171. Eukaryota.
COG2114. LUCA.
HOVERGENiHBG106603.
InParanoidiP19686.

Enzyme and pathway databases

BRENDAi4.6.1.2. 5301.

Miscellaneous databases

PROiP19686.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011645. HNOB_dom_associated.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCYA3_RAT
AccessioniPrimary (citable) accession number: P19686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.