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Protein

Pyruvate kinase

Gene

pyk

Organism
Spiroplasma citri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathway:iglycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk), Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411SubstrateBy similarity
Metal bindingi43 – 431PotassiumBy similarity
Metal bindingi45 – 451PotassiumBy similarity
Metal bindingi75 – 751PotassiumBy similarity
Metal bindingi76 – 761Potassium; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
OrganismiSpiroplasma citri
Taxonomic identifieri2133 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesEntomoplasmatalesSpiroplasmataceaeSpiroplasma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›192›192Pyruvate kinasePRO_0000112084Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP19680.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR015793. Pyrv_Knase_brl.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.

Sequencei

Sequence statusi: Fragment.

P19680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKFNINEKM KRTKIITTIG PSTHSPGAIE ELFKTGMTTI RLNFSHGDHA
60 70 80 90 100
EQGARIVWAR EVSAKIGKPI SVLLDTKGPE IHWRIKGLLQ KSLNIYHQMN
110 120 130 140 150
NSLMFSPSFC KFLHYHYTLL LSKKTKKQLL KIVKKLKKLQ SQHYNLAHGL
160 170 180 190
RETWTSSFCF NERPSIWRLL SSFKQIVTSF KISLVSKVLI KL
Length:192
Mass (Da):22,209
Last modified:February 1, 1991 - v1
Checksum:iACF22444BF2CF807
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 192111HWRIK…VLIKL → RAGIMKGGKQEIVAGATVTI YSLPTEYQNREGTGTEITVS YDMSQDLKVGDVVLVDDGKL QLNVTGIKPGIIETKA in AAB69999 (PubMed:9732535).CuratedAdd
BLAST
Non-terminal residuei192 – 1921

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012877 Genomic DNA. Translation: AAB69999.1.
PIRiF35270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012877 Genomic DNA. Translation: AAB69999.1.
PIRiF35270.

3D structure databases

ProteinModelPortaliP19680.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR015793. Pyrv_Knase_brl.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein."
    Chevalier C., Saillard C., Bove J.M.
    J. Bacteriol. 172:2693-2703(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27556 / NCPPB 2647 / R8A2.
  2. "Gene organization and transcriptional analysis of the Spiroplasma citri rpsB/tsf/x operon."
    Le Dantec L., Bove J.M., Saillard C.
    Curr. Microbiol. 37:269-273(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKPYK_SPICI
AccessioniPrimary (citable) accession number: P19680
Secondary accession number(s): O30600
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.