P19680 (KPYK_SPICI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Name:

pyk

Organism

Spiroplasma citri

Taxonomic identifier

2133 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Entomoplasmatales › Spiroplasmataceae › Spiroplasma

Protein attributes

Sequence length	192 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›192	›192	Pyruvate kinase		PRO_0000112084
Sites
	Metal binding	43	1	Potassium By similarity
	Metal binding	45	1	Potassium By similarity
	Metal binding	75	1	Potassium By similarity
	Metal binding	76	1	Potassium; via carbonyl oxygen By similarity
	Binding site	41	1	Substrate By similarity
Experimental info
	Sequence conflict	82 – 192	111	HWRIK…VLIKL → RAGIMKGGKQEIVAGATVTI YSLPTEYQNREGTGTEITVS YDMSQDLKVGDVVLVDDGKL QLNVTGIKPGIIETKA in AAB69999. Ref.2
	Non-terminal residue	192	1

Sequences

	Sequence		Length	Mass (Da)
	P19680 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: ACF22444BF2CF807	FASTA	192	22,209
	10 20 30 40 50 60 MDKFNINEKM KRTKIITTIG PSTHSPGAIE ELFKTGMTTI RLNFSHGDHA EQGARIVWAR 70 80 90 100 110 120 EVSAKIGKPI SVLLDTKGPE IHWRIKGLLQ KSLNIYHQMN NSLMFSPSFC KFLHYHYTLL 130 140 150 160 170 180 LSKKTKKQLL KIVKKLKKLQ SQHYNLAHGL RETWTSSFCF NERPSIWRLL SSFKQIVTSF 190 KISLVSKVLI KL

References

[1]	"Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein." Chevalier C., Saillard C., Bove J.M. J. Bacteriol. 172:2693-2703(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27556 / NCPPB 2647 / R8A2.
[2]	"Gene organization and transcriptional analysis of the Spiroplasma citri rpsB/tsf/x operon." Le Dantec L., Bove J.M., Saillard C. Curr. Microbiol. 37:269-273(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF012877 Genomic DNA. Translation: AAB69999.1.
PIR	F35270.
3D structure databases
ProteinModelPortal	P19680.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	3.20.20.60. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. IPR015793. Pyrv_Knase_brl. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. [Graphical view]
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_SPICI

Accession

Primary (citable) accession number: P19680
Secondary accession number(s): O30600

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	May 29, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program