Reviewed, UniProtKB/Swiss-Prot P19668 (BGAL_BACST)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase 1
      Short name=Beta-gal
    EC=3.2.1.23
Alternative name(s):
    Beta-galactosidase I
    Lactase

Gene names

Name:

bgaB

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	672 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Sequence similarities	Belongs to the glycosyl hydrolase 42 family.
biophysicochemical properties	Temperature dependence: Beta-galactosidase I is more thermostable than the II, and the III isoforms, being stable up to 70 degrees Celsius.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 672	672	Beta-galactosidase 1		PRO_0000057691
Sites
	Active site	148	1	Proton donor Potential
	Active site	303	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	P19668-1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: FF611FFBEF68F09A	FASTA	672	78,053
	10 20 30 40 50 60 MNVLSSICYG GDYNPEQWPE EIWYEDAKLM QKAGVNLVSL GIFSWSKIEP SDGVFDFEWL 70 80 90 100 110 120 DKVIDILYDH GVYINLGTAT ATTPAWFVKK YPDSLPIDES GVILSFGSRQ HYCPNHPQLI 130 140 150 160 170 180 THIKRLVRAI AERYKNHPAL KMWHVNNEYA CHVSKCFCEN CAVAFRKWLK ERYKTIDELN 190 200 210 220 230 240 ERWGTNFWGQ RYNHWDEINP PRKAPTFINP SQELDYYRFM NDSILKLFLT EKEILREVTP 250 260 270 280 290 300 DIPVSTNFMG SFKPLNYFQW AQHVDIVTWD SYPDPREGLP IQHAMMNDLM RSLRKGQPFI 310 320 330 340 350 360 LMEQVTSHVN WRDINVPKPP GVMRLWSYAT IARGADGIMF FQWRQSRAGA EKFHGAMVPH 370 380 390 400 410 420 FLNENNRIYR EVTQLGQELK KLDCLVGSRI KAEVAIIFDW ENWWAVELSS KPHNKLRYIP 430 440 450 460 470 480 IVEAYYRELY KRNIAVDFVR PSDDLTKYKV VIAPMLYMVK EGEDENLRQF VANGGTLIVS 490 500 510 520 530 540 FFSGIVDEND RVHLGGYPGP LRDILGIFVE EFVPYPETKV NKIYSNDGEY DCTTWADIIR 550 560 570 580 590 600 LEGAEPLATF KGDWYAGLPA VTRNCYGKGE GIYVGTYPDS NYLGRLLEQV FAKHHINPIL 610 620 630 640 650 660 EVAENVEVQQ RETDEWKYLI IINHNDYEVT LSLPEDKIYQ NMIDGKCFRG GELRIQGVDV 670 AVLREHDEAG KV

References

[1]	"Structure of a beta-galactosidase gene of Bacillus stearothermophilus." Hirata H., Fukazawa T., Negoro S., Okada H. J. Bacteriol. 166:722-727(1986) [PubMed: 3086288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14. Strain: ATCC 8005 / IAM11001.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M13466 Genomic DNA. Translation: AAA22262.1.
PIR	A29836.
3D structure databases
HSSP	HSSP built from PDB template 1KWG based on UniProtKB O69315.
ModBase	Search...
Protein family/group databases
CAZy	GH42. Glycoside Hydrolase Family 42.
Enzyme and pathway databases
BRENDA	3.2.1.23. 266715.
Family and domain databases
InterPro	IPR013739. Beta_Galactosidase_C. IPR013738. Beta_Galactosidase_Trimer. IPR003476. Glyco_hydro_42. IPR013529. Glyco_hydro_42_N. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02449. Glyco_hydro_42. 1 hit. PF08533. Glyco_hydro_42C. 1 hit. PF08532. Glyco_hydro_42M. 1 hit. [Graphical view]
PIRSF	PIRSF001084. B-galactosidase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BGAL_BACST

Accession

Primary (citable) accession number: P19668

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)