UniProtKB - P19659 (MED15_YEAST)
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Protein
Mediator of RNA polymerase II transcription subunit 15
Gene
GAL11
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.3 Publications
Miscellaneous
Present with 606 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- RNA polymerase II activating transcription factor binding Source: SGD
- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly Source: SGD
- TFIIE-class transcription factor binding Source: SGD
- TFIIH-class transcription factor binding Source: SGD
- transcription factor activity, RNA polymerase II transcription factor recruiting Source: SGD
- transcription factor activity, TFIIE-class binding Source: SGD
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: SGD
- positive regulation of invasive growth in response to glucose limitation Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
- regulation of establishment of protein localization to chromosome Source: SGD
- RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
Keywordsi
Molecular function | Activator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | YEAST:G3O-33462-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 15Alternative name(s): Autonomous replication regulatory protein 3 Basal expression activator protein 1 Defective silencing suppressor protein 4 Mediator complex subunit 15 Transcription regulatory protein GAL11 Ty insertion suppressor protein 13 |
Gene namesi | Name:GAL11 Synonyms:ABE1, MED15, RAR3, SDS4, SPT13 Ordered Locus Names:YOL051W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YOL051W. |
SGDi | S000005411. GAL11. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000096363 | 2 – 1081 | Mediator of RNA polymerase II transcription subunit 15Add BLAST | 1080 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 335 | PhosphoserineCombined sources | 1 | |
Modified residuei | 736 | PhosphoserineCombined sources | 1 | |
Modified residuei | 752 | PhosphoserineCombined sources | 1 | |
Modified residuei | 783 | PhosphoserineCombined sources | 1 | |
Modified residuei | 785 | PhosphoserineCombined sources | 1 | |
Modified residuei | 789 | PhosphoserineCombined sources | 1 | |
Modified residuei | 793 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 831 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1003 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1008 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1018 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1034 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P19659. |
PaxDbi | P19659. |
PRIDEi | P19659. |
PTM databases
iPTMneti | P19659. |
Interactioni
Subunit structurei
Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. GAL11/MED15 interacts directly with the activator GAL4.2 Publications
Binary interactionsi
GO - Molecular functioni
- RNA polymerase II activating transcription factor binding Source: SGD
- TFIIE-class transcription factor binding Source: SGD
- TFIIH-class transcription factor binding Source: SGD
Protein-protein interaction databases
BioGridi | 34353. 163 interactors. |
DIPi | DIP-2334N. |
IntActi | P19659. 35 interactors. |
MINTi | P19659. |
STRINGi | 4932.YOL051W. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Beta strandi | 8 – 11 | Combined sources | 4 | |
Helixi | 14 – 35 | Combined sources | 22 | |
Helixi | 41 – 58 | Combined sources | 18 | |
Helixi | 63 – 89 | Combined sources | 27 | |
Helixi | 164 – 170 | Combined sources | 7 | |
Helixi | 172 – 174 | Combined sources | 3 | |
Helixi | 180 – 183 | Combined sources | 4 | |
Helixi | 196 – 199 | Combined sources | 4 | |
Helixi | 201 – 204 | Combined sources | 4 | |
Helixi | 210 – 232 | Combined sources | 23 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2K0N | NMR | - | A | 6-90 | [»] | |
2LPB | NMR | - | A | 158-238 | [»] | |
ProteinModelPortali | P19659. | |||||
SMRi | P19659. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P19659. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 422 – 423 | 1 | 2 | |
Repeati | 424 – 425 | 2 | 2 | |
Repeati | 426 – 427 | 3 | 2 | |
Repeati | 428 – 429 | 4 | 2 | |
Repeati | 430 – 431 | 5 | 2 | |
Repeati | 432 – 433 | 6 | 2 | |
Repeati | 434 – 435 | 7 | 2 | |
Repeati | 436 – 437 | 8 | 2 | |
Repeati | 438 – 439 | 9 | 2 | |
Repeati | 440 – 441 | 10 | 2 | |
Repeati | 442 – 443 | 11 | 2 | |
Repeati | 444 – 445 | 12; approximate | 2 | |
Repeati | 446 – 447 | 13; approximate | 2 | |
Repeati | 448 – 449 | 14 | 2 | |
Repeati | 450 – 451 | 15 | 2 | |
Repeati | 452 – 453 | 16 | 2 | |
Repeati | 454 – 455 | 17 | 2 | |
Repeati | 456 – 457 | 18 | 2 | |
Repeati | 458 – 459 | 19 | 2 | |
Repeati | 460 – 461 | 20 | 2 | |
Repeati | 462 – 463 | 21 | 2 | |
Repeati | 464 – 465 | 22 | 2 | |
Repeati | 466 – 467 | 23 | 2 | |
Repeati | 468 – 469 | 24 | 2 | |
Repeati | 470 – 471 | 25 | 2 | |
Repeati | 472 – 473 | 26 | 2 | |
Repeati | 474 – 475 | 27 | 2 | |
Repeati | 476 – 477 | 28 | 2 | |
Repeati | 478 – 479 | 29 | 2 | |
Repeati | 480 – 481 | 30 | 2 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 422 – 481 | 30 X 2 AA approximate tandem repeats of Q-AAdd BLAST | 60 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 147 – 158 | Poly-GlnAdd BLAST | 12 | |
Compositional biasi | 674 – 696 | Poly-GlnAdd BLAST | 23 |
Sequence similaritiesi
Belongs to the Mediator complex subunit 15 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
HOGENOMi | HOG000113522. |
InParanoidi | P19659. |
KOi | K15158. |
OMAi | RFKHRQE. |
OrthoDBi | EOG092C0QLI. |
Family and domain databases
CDDi | cd12191. gal11_coact. 1 hit. |
InterProi | View protein in InterPro IPR033789. Gal11_coact. IPR036546. MED15_KIX. IPR008626. Mediator_Med15_fun. |
Pfami | View protein in Pfam PF16987. KIX_2. 1 hit. PF05397. Med15_fungi. 1 hit. |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P19659-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAAPVQDKD TLSNAERAKN VNGLLQVLMD INTLNGGSSD TADKIRIHAK
60 70 80 90 100
NFEAALFAKS SSKKEYMDSM NEKVAVMRNT YNTRKNAVTA AAANNNIKPV
110 120 130 140 150
EQHHINNLKN SGNSANNMNV NMNLNPQMFL NQQAQARQQV AQQLRNQQQQ
160 170 180 190 200
QQQQQQQQRR QLTPQQQQLV NQMKVAPIPK QLLQRIPNIP PNINTWQQVT
210 220 230 240 250
ALAQQKLLTP QDMEAAKEVY KIHQQLLFKA RLQQQQAQAQ AQANNNNNGL
260 270 280 290 300
PQNGNINNNI NIPQQQQMQP PNSSANNNPL QQQSSQNTVP NVLNQINQIF
310 320 330 340 350
SPEEQRSLLQ EAIETCKNFE KTQLGSTMTE PVKQSFIRKY INQKALRKIQ
360 370 380 390 400
ALRDVKNNNN ANNNGSNLQR AQNVPMNIIQ QQQQQNTNNN DTIATSATPN
410 420 430 440 450
AAAFSQQQNA SSKLYQMQQQ QQAQAQAQAQ AQAQAQAQAQ AQAAQAAQAQ
460 470 480 490 500
AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AHAQHQPSQQ PQQAQQQPNP
510 520 530 540 550
LHGLTPTAKD VEVIKQLSLD ASKTNLRLTD VTNSLSNEEK EKIKMKLKQG
560 570 580 590 600
QKLFVQVSNF APQVYIITKN ENFLKEVFQL RIFVKEILEK CAEGIFVVKL
610 620 630 640 650
DTVDRLIIKY QKYWESMRIQ ILRRQAILRQ QQQMANNNGN PGTTSTGNNN
660 670 680 690 700
NIATQQNMQQ SLQQMQHLQQ LKMQQQQQQQ QQQQQQQQQQ QQQQQQHIYP
710 720 730 740 750
SSTPGVANYS AMANAPGNNI PYMNHKNTSS MDFLNSMENT PKVPVSAAAT
760 770 780 790 800
PSLNKTINGK VNGRTKSNTI PVTSIPSTNK KLSISNAASQ QPTPRSASNT
810 820 830 840 850
AKSTPNTNPS PLKTQTKNGT PNPNNMKTVQ SPMGAQPSYN SAIIENAFRK
860 870 880 890 900
EELLLKDLEI RKLEISSRFK HRQEIFKDSP MDLFMSTLGD CLGIKDEEML
910 920 930 940 950
TSCTIPKAVV DHINGSGKRK PTKAAQRARD QDSIDISIKD NKLVMKSKFN
960 970 980 990 1000
KSNRSYSIAL SNVAAIFKGI GGNFKDLSTL VHSSSPSTSS NMDVGNPRKR
1010 1020 1030 1040 1050
KASVLEISPQ DSIASVLSPD SNIMSDSKKI KVDSPDDPFM TKSGATTSEK
1060 1070 1080
QEVTNEAPFL TSGTSSEQFN VWDWNNWTSA T
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 171 | N → T in AAA34622 (PubMed:3062377).Curated | 1 | |
Sequence conflicti | 302 | P → Q in AAA34622 (PubMed:3062377).Curated | 1 | |
Sequence conflicti | 499 | N → T in AAA34622 (PubMed:3062377).Curated | 1 | |
Sequence conflicti | 751 | P → Q in AAA34622 (PubMed:3062377).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M22481 Genomic DNA. Translation: AAA34622.1. Z74793 Genomic DNA. Translation: CAA99056.1. X91067 Genomic DNA. Translation: CAA62537.1. BK006948 Genomic DNA. Translation: DAA10733.1. |
PIRi | S66736. |
RefSeqi | NP_014591.1. NM_001183305.1. |
Genome annotation databases
EnsemblFungii | YOL051W; YOL051W; YOL051W. |
GeneIDi | 854106. |
KEGGi | sce:YOL051W. |
Similar proteinsi
Entry informationi
Entry namei | MED15_YEAST | |
Accessioni | P19659Primary (citable) accession number: P19659 Secondary accession number(s): D6W217, Q08221 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | November 1, 1997 | |
Last modified: | March 28, 2018 | |
This is version 169 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |