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P19658 (EXO70_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exocyst complex component EXO70
Alternative name(s):
Exocyst complex protein of 70 kDa
Gene names
Name:EXO70
Ordered Locus Names:YJL085W
ORF Names:J0932
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length623 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in the assembly of the exocyst. Ref.11

Subunit structure

The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Interacts with RHO3. Ref.1 Ref.8 Ref.14

Subcellular location

Bud. Bud neck. Note: Its polarization in the cell depends on EXO84. Ref.9 Ref.12 Ref.13

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the EXO70 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RHO3Q002453EBI-6717,EBI-15138

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 623623Exocyst complex component EXO70
PRO_0000118975

Experimental info

Sequence conflict4971A → S in AAU09750. Ref.6

Secondary structure

............................................................ 623
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P19658 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 277D89176FC2837D

FASTA62371,294
        10         20         30         40         50         60 
MPAEIDIDEA DVLVLSQELQ KTSKLTFEIN KSLKKIAATS NQSSQLFTPI LARNNVLTTL 

        70         80         90        100        110        120 
QRNIESTLNS VASVKDLANE ASKYEIILQK GINQVGLKQY TQVVHKLDDM LEDIQSGQAN 

       130        140        150        160        170        180 
REENSEFHGI LTHLEQLIKR SEAQLRVYFI SILNSIKPFD PQINITKKMP FPYYEDQQLG 

       190        200        210        220        230        240 
ALSWILDYFH GNSEGSIIQD ILVGERSKLI LKCMAFLEPF AKEISTAKNA PYEKGSSGMN 

       250        260        270        280        290        300 
SYTEALLGFI ANEKSLVDDL YSQYTESKPH VLSQILSPLI SAYAKLFGAN LKIVRSNLEN 

       310        320        330        340        350        360 
FGFFSFELVE SINDVKKSLR GKELQNYNLL QDCTQEVRQV TQSLFRDAID RIIKKANSIS 

       370        380        390        400        410        420 
TIPSNNGVTE ATVDTMSRLR KFSEYKNGCL GAMDNITREN WLPSNYKEKE YTLQNEALNW 

       430        440        450        460        470        480 
EDHNVLLSCF ISDCIDTLAV NLERKAQIAL MPNQEPDVAN PNSSKNKHKQ RIGFFILMNL 

       490        500        510        520        530        540 
TLVEQIVEKS ELNLMLAGEG HSRLERLKKR YISYMVSDWR DLTANLMDSV FIDSSGKKSK 

       550        560        570        580        590        600 
DKEQIKEKFR KFNEGFEDLV SKTKQYKLSD PSLKVTLKSE IISLVMPMYE RFYSRYKDSF 

       610        620 
KNPRKHIKYT PDELTTVLNQ LVR 

« Hide

References

« Hide 'large scale' references
[1]"The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae."
TerBush D.R., Maurice T., Roth D., Novick P.
EMBO J. 15:6483-6494(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-52, IDENTIFICATION IN THE EXOCYST COMPLEX.
[2]"Nucleotide sequence of ORF2: an open reading frame upstream of the tRNA ligase gene."
Komatsoulis G.A., Westaway S.K., Abelson J.
Nucleic Acids Res. 15:9079-9079(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]"Structure and function of the yeast tRNA ligase gene."
Westaway S.K., Phizicky E.M., Abelson J.
J. Biol. Chem. 263:3171-3176(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70.
Strain: EJ101.
[8]"Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70."
Robinson N.G.G., Guo L., Imai J., Toh-e A., Matsui Y., Tamanoi F.
Mol. Cell. Biol. 19:3580-3587(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RHO3.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p."
Wiederkehr A., De Craene J.-O., Ferro-Novick S., Novick P.
J. Cell Biol. 167:875-887(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p."
Boyd C., Hughes T., Pypaert M., Novick P.
J. Cell Biol. 167:889-901(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[13]"The critical role of EXO84P in the organization and polarized localization of the exocyst complex."
Zhang X., Zajac A., Zhang J., Wang P., Li M., Murray J., TerBush D.R., Guo W.
J. Biol. Chem. 280:20356-20364(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[14]"The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif."
Dong G., Hutagalung A.H., Fu C., Novick P., Reinisch K.M.
Nat. Struct. Mol. Biol. 12:1094-1100(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 63-623, INTERACTION WITH RHO3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y08787 Genomic DNA. Translation: CAA70039.1.
Y00473 Genomic DNA. Translation: CAA68535.1.
X83502 Genomic DNA. Translation: CAA58485.1.
Z49362 Genomic DNA. Translation: CAA89380.1.
AY723833 Genomic DNA. Translation: AAU09750.1.
J03546 Genomic DNA. Translation: AAA66919.1.
BK006943 Genomic DNA. Translation: DAA08714.1.
PIRA27300.
RefSeqNP_012450.1. NM_001181518.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2B1EX-ray2.00A63-623[»]
2B7MX-ray3.50A/B/C/D63-623[»]
2PFVX-ray2.10A63-623[»]
ProteinModelPortalP19658.
SMRP19658. Positions 67-623.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33671. 87 interactions.
DIPDIP-2934N.
IntActP19658. 9 interactions.
MINTMINT-700581.
STRING4932.YJL085W.

Proteomic databases

MaxQBP19658.
PaxDbP19658.
PeptideAtlasP19658.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL085W; YJL085W; YJL085W.
GeneID853359.
KEGGsce:YJL085W.

Organism-specific databases

CYGDYJL085w.
SGDS000003621. EXO70.

Phylogenomic databases

eggNOGNOG245075.
HOGENOMHOG000000807.
KOK07195.
OMAPRKHIKY.
OrthoDBEOG790G8M.

Enzyme and pathway databases

BioCycYEAST:G3O-31542-MONOMER.

Gene expression databases

GenevestigatorP19658.

Family and domain databases

InterProIPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view]
PANTHERPTHR12542. PTHR12542. 1 hit.
PfamPF03081. Exo70. 1 hit.
[Graphical view]
SUPFAMSSF74788. SSF74788. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP19658.
NextBio973780.
PROP19658.

Entry information

Entry nameEXO70_YEAST
AccessionPrimary (citable) accession number: P19658
Secondary accession number(s): D6VW98, E9P961
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references