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P19658

- EXO70_YEAST

UniProt

P19658 - EXO70_YEAST

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Protein

Exocyst complex component EXO70

Gene

EXO70

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in the assembly of the exocyst.1 Publication

GO - Molecular functioni

  1. GTP-Rho binding Source: SGD
  2. phosphatidylinositol-4,5-bisphosphate binding Source: SGD

GO - Biological processi

  1. exocyst assembly Source: SGD
  2. exocyst localization Source: SGD
  3. exocytosis Source: SGD
  4. Golgi to plasma membrane transport Source: SGD
  5. protein transport Source: UniProtKB-KW
  6. Rho protein signal transduction Source: SGD
  7. vesicle tethering involved in exocytosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31542-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exocyst complex component EXO70
Alternative name(s):
Exocyst complex protein of 70 kDa
Gene namesi
Name:EXO70
Ordered Locus Names:YJL085W
ORF Names:J0932
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL085w.
SGDiS000003621. EXO70.

Subcellular locationi

Bud. Bud neck
Note: Its polarization in the cell depends on EXO84.

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. exocyst Source: SGD
  4. incipient cellular bud site Source: SGD
  5. plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Exocyst complex component EXO70PRO_0000118975Add
BLAST

Proteomic databases

MaxQBiP19658.
PaxDbiP19658.
PeptideAtlasiP19658.

Expressioni

Gene expression databases

GenevestigatoriP19658.

Interactioni

Subunit structurei

The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Interacts with RHO3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RHO3Q002453EBI-6717,EBI-15138

Protein-protein interaction databases

BioGridi33671. 87 interactions.
DIPiDIP-2934N.
IntActiP19658. 9 interactions.
MINTiMINT-700581.
STRINGi4932.YJL085W.

Structurei

Secondary structure

1
623
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi69 – 713Combined sources
Helixi74 – 8916Combined sources
Helixi92 – 954Combined sources
Helixi97 – 11519Combined sources
Beta strandi116 – 1194Combined sources
Turni122 – 1243Combined sources
Helixi125 – 15329Combined sources
Helixi161 – 1677Combined sources
Helixi176 – 19116Combined sources
Helixi195 – 21420Combined sources
Turni215 – 2173Combined sources
Helixi218 – 2214Combined sources
Helixi239 – 26022Combined sources
Helixi268 – 29225Combined sources
Helixi298 – 3014Combined sources
Helixi304 – 31815Combined sources
Turni319 – 3213Combined sources
Helixi327 – 35832Combined sources
Helixi370 – 38213Combined sources
Helixi383 – 3853Combined sources
Helixi386 – 3927Combined sources
Turni393 – 3953Combined sources
Helixi398 – 4003Combined sources
Helixi408 – 4103Combined sources
Helixi423 – 45028Combined sources
Helixi469 – 48820Combined sources
Helixi492 – 4954Combined sources
Turni496 – 4983Combined sources
Helixi499 – 53234Combined sources
Beta strandi535 – 5373Combined sources
Helixi542 – 56322Combined sources
Helixi571 – 59626Combined sources
Helixi597 – 5993Combined sources
Beta strandi600 – 6023Combined sources
Helixi603 – 6053Combined sources
Helixi611 – 62212Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B1EX-ray2.00A63-623[»]
2B7MX-ray3.50A/B/C/D63-623[»]
2PFVX-ray2.10A63-623[»]
ProteinModelPortaliP19658.
SMRiP19658. Positions 67-623.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19658.

Family & Domainsi

Sequence similaritiesi

Belongs to the EXO70 family.Curated

Phylogenomic databases

eggNOGiNOG245075.
HOGENOMiHOG000000807.
InParanoidiP19658.
KOiK07195.
OMAiPRKHIKY.
OrthoDBiEOG790G8M.

Family and domain databases

InterProiIPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view]
PANTHERiPTHR12542. PTHR12542. 1 hit.
PfamiPF03081. Exo70. 1 hit.
[Graphical view]
SUPFAMiSSF74788. SSF74788. 1 hit.

Sequencei

Sequence statusi: Complete.

P19658-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPAEIDIDEA DVLVLSQELQ KTSKLTFEIN KSLKKIAATS NQSSQLFTPI
60 70 80 90 100
LARNNVLTTL QRNIESTLNS VASVKDLANE ASKYEIILQK GINQVGLKQY
110 120 130 140 150
TQVVHKLDDM LEDIQSGQAN REENSEFHGI LTHLEQLIKR SEAQLRVYFI
160 170 180 190 200
SILNSIKPFD PQINITKKMP FPYYEDQQLG ALSWILDYFH GNSEGSIIQD
210 220 230 240 250
ILVGERSKLI LKCMAFLEPF AKEISTAKNA PYEKGSSGMN SYTEALLGFI
260 270 280 290 300
ANEKSLVDDL YSQYTESKPH VLSQILSPLI SAYAKLFGAN LKIVRSNLEN
310 320 330 340 350
FGFFSFELVE SINDVKKSLR GKELQNYNLL QDCTQEVRQV TQSLFRDAID
360 370 380 390 400
RIIKKANSIS TIPSNNGVTE ATVDTMSRLR KFSEYKNGCL GAMDNITREN
410 420 430 440 450
WLPSNYKEKE YTLQNEALNW EDHNVLLSCF ISDCIDTLAV NLERKAQIAL
460 470 480 490 500
MPNQEPDVAN PNSSKNKHKQ RIGFFILMNL TLVEQIVEKS ELNLMLAGEG
510 520 530 540 550
HSRLERLKKR YISYMVSDWR DLTANLMDSV FIDSSGKKSK DKEQIKEKFR
560 570 580 590 600
KFNEGFEDLV SKTKQYKLSD PSLKVTLKSE IISLVMPMYE RFYSRYKDSF
610 620
KNPRKHIKYT PDELTTVLNQ LVR
Length:623
Mass (Da):71,294
Last modified:February 1, 1991 - v1
Checksum:i277D89176FC2837D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti497 – 4971A → S in AAU09750. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08787 Genomic DNA. Translation: CAA70039.1.
Y00473 Genomic DNA. Translation: CAA68535.1.
X83502 Genomic DNA. Translation: CAA58485.1.
Z49362 Genomic DNA. Translation: CAA89380.1.
AY723833 Genomic DNA. Translation: AAU09750.1.
J03546 Genomic DNA. Translation: AAA66919.1.
BK006943 Genomic DNA. Translation: DAA08714.1.
PIRiA27300.
RefSeqiNP_012450.1. NM_001181518.1.

Genome annotation databases

EnsemblFungiiYJL085W; YJL085W; YJL085W.
GeneIDi853359.
KEGGisce:YJL085W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08787 Genomic DNA. Translation: CAA70039.1 .
Y00473 Genomic DNA. Translation: CAA68535.1 .
X83502 Genomic DNA. Translation: CAA58485.1 .
Z49362 Genomic DNA. Translation: CAA89380.1 .
AY723833 Genomic DNA. Translation: AAU09750.1 .
J03546 Genomic DNA. Translation: AAA66919.1 .
BK006943 Genomic DNA. Translation: DAA08714.1 .
PIRi A27300.
RefSeqi NP_012450.1. NM_001181518.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2B1E X-ray 2.00 A 63-623 [» ]
2B7M X-ray 3.50 A/B/C/D 63-623 [» ]
2PFV X-ray 2.10 A 63-623 [» ]
ProteinModelPortali P19658.
SMRi P19658. Positions 67-623.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33671. 87 interactions.
DIPi DIP-2934N.
IntActi P19658. 9 interactions.
MINTi MINT-700581.
STRINGi 4932.YJL085W.

Proteomic databases

MaxQBi P19658.
PaxDbi P19658.
PeptideAtlasi P19658.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL085W ; YJL085W ; YJL085W .
GeneIDi 853359.
KEGGi sce:YJL085W.

Organism-specific databases

CYGDi YJL085w.
SGDi S000003621. EXO70.

Phylogenomic databases

eggNOGi NOG245075.
HOGENOMi HOG000000807.
InParanoidi P19658.
KOi K07195.
OMAi PRKHIKY.
OrthoDBi EOG790G8M.

Enzyme and pathway databases

BioCyci YEAST:G3O-31542-MONOMER.

Miscellaneous databases

EvolutionaryTracei P19658.
NextBioi 973780.
PROi P19658.

Gene expression databases

Genevestigatori P19658.

Family and domain databases

InterProi IPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view ]
PANTHERi PTHR12542. PTHR12542. 1 hit.
Pfami PF03081. Exo70. 1 hit.
[Graphical view ]
SUPFAMi SSF74788. SSF74788. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae."
    TerBush D.R., Maurice T., Roth D., Novick P.
    EMBO J. 15:6483-6494(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-52, IDENTIFICATION IN THE EXOCYST COMPLEX.
  2. "Nucleotide sequence of ORF2: an open reading frame upstream of the tRNA ligase gene."
    Komatsoulis G.A., Westaway S.K., Abelson J.
    Nucleic Acids Res. 15:9079-9079(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
    Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
    Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Structure and function of the yeast tRNA ligase gene."
    Westaway S.K., Phizicky E.M., Abelson J.
    J. Biol. Chem. 263:3171-3176(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70.
    Strain: EJ101.
  8. "Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70."
    Robinson N.G.G., Guo L., Imai J., Toh-e A., Matsui Y., Tamanoi F.
    Mol. Cell. Biol. 19:3580-3587(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RHO3.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p."
    Wiederkehr A., De Craene J.-O., Ferro-Novick S., Novick P.
    J. Cell Biol. 167:875-887(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p."
    Boyd C., Hughes T., Pypaert M., Novick P.
    J. Cell Biol. 167:889-901(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  13. "The critical role of EXO84P in the organization and polarized localization of the exocyst complex."
    Zhang X., Zajac A., Zhang J., Wang P., Li M., Murray J., TerBush D.R., Guo W.
    J. Biol. Chem. 280:20356-20364(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  14. "The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif."
    Dong G., Hutagalung A.H., Fu C., Novick P., Reinisch K.M.
    Nat. Struct. Mol. Biol. 12:1094-1100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 63-623, INTERACTION WITH RHO3.

Entry informationi

Entry nameiEXO70_YEAST
AccessioniPrimary (citable) accession number: P19658
Secondary accession number(s): D6VW98, E9P961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 26, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3