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P19658

- EXO70_YEAST

UniProt

P19658 - EXO70_YEAST

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Protein
Exocyst complex component EXO70
Gene
EXO70, YJL085W, J0932
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in the assembly of the exocyst.1 Publication

GO - Molecular functioni

  1. GTP-Rho binding Source: SGD
  2. phosphatidylinositol-4,5-bisphosphate binding Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. Golgi to plasma membrane transport Source: SGD
  2. Rho protein signal transduction Source: SGD
  3. exocyst assembly Source: SGD
  4. exocyst localization Source: SGD
  5. exocytosis Source: SGD
  6. protein transport Source: UniProtKB-KW
  7. vesicle tethering involved in exocytosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31542-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exocyst complex component EXO70
Alternative name(s):
Exocyst complex protein of 70 kDa
Gene namesi
Name:EXO70
Ordered Locus Names:YJL085W
ORF Names:J0932
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL085w.
SGDiS000003621. EXO70.

Subcellular locationi

Bud. Bud neck
Note: Its polarization in the cell depends on EXO84.3 Publications

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. exocyst Source: SGD
  4. incipient cellular bud site Source: SGD
  5. plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Exocyst complex component EXO70
PRO_0000118975Add
BLAST

Proteomic databases

MaxQBiP19658.
PaxDbiP19658.
PeptideAtlasiP19658.

Expressioni

Gene expression databases

GenevestigatoriP19658.

Interactioni

Subunit structurei

The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Interacts with RHO3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RHO3Q002453EBI-6717,EBI-15138

Protein-protein interaction databases

BioGridi33671. 87 interactions.
DIPiDIP-2934N.
IntActiP19658. 9 interactions.
MINTiMINT-700581.
STRINGi4932.YJL085W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi69 – 713
Helixi74 – 8916
Helixi92 – 954
Helixi97 – 11519
Beta strandi116 – 1194
Turni122 – 1243
Helixi125 – 15329
Helixi161 – 1677
Helixi176 – 19116
Helixi195 – 21420
Turni215 – 2173
Helixi218 – 2214
Helixi239 – 26022
Helixi268 – 29225
Helixi298 – 3014
Helixi304 – 31815
Turni319 – 3213
Helixi327 – 35832
Helixi370 – 38213
Helixi383 – 3853
Helixi386 – 3927
Turni393 – 3953
Helixi398 – 4003
Helixi408 – 4103
Helixi423 – 45028
Helixi469 – 48820
Helixi492 – 4954
Turni496 – 4983
Helixi499 – 53234
Beta strandi535 – 5373
Helixi542 – 56322
Helixi571 – 59626
Helixi597 – 5993
Beta strandi600 – 6023
Helixi603 – 6053
Helixi611 – 62212

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B1EX-ray2.00A63-623[»]
2B7MX-ray3.50A/B/C/D63-623[»]
2PFVX-ray2.10A63-623[»]
ProteinModelPortaliP19658.
SMRiP19658. Positions 67-623.

Miscellaneous databases

EvolutionaryTraceiP19658.

Family & Domainsi

Sequence similaritiesi

Belongs to the EXO70 family.

Phylogenomic databases

eggNOGiNOG245075.
HOGENOMiHOG000000807.
KOiK07195.
OMAiPRKHIKY.
OrthoDBiEOG790G8M.

Family and domain databases

InterProiIPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view]
PANTHERiPTHR12542. PTHR12542. 1 hit.
PfamiPF03081. Exo70. 1 hit.
[Graphical view]
SUPFAMiSSF74788. SSF74788. 1 hit.

Sequencei

Sequence statusi: Complete.

P19658-1 [UniParc]FASTAAdd to Basket

« Hide

MPAEIDIDEA DVLVLSQELQ KTSKLTFEIN KSLKKIAATS NQSSQLFTPI    50
LARNNVLTTL QRNIESTLNS VASVKDLANE ASKYEIILQK GINQVGLKQY 100
TQVVHKLDDM LEDIQSGQAN REENSEFHGI LTHLEQLIKR SEAQLRVYFI 150
SILNSIKPFD PQINITKKMP FPYYEDQQLG ALSWILDYFH GNSEGSIIQD 200
ILVGERSKLI LKCMAFLEPF AKEISTAKNA PYEKGSSGMN SYTEALLGFI 250
ANEKSLVDDL YSQYTESKPH VLSQILSPLI SAYAKLFGAN LKIVRSNLEN 300
FGFFSFELVE SINDVKKSLR GKELQNYNLL QDCTQEVRQV TQSLFRDAID 350
RIIKKANSIS TIPSNNGVTE ATVDTMSRLR KFSEYKNGCL GAMDNITREN 400
WLPSNYKEKE YTLQNEALNW EDHNVLLSCF ISDCIDTLAV NLERKAQIAL 450
MPNQEPDVAN PNSSKNKHKQ RIGFFILMNL TLVEQIVEKS ELNLMLAGEG 500
HSRLERLKKR YISYMVSDWR DLTANLMDSV FIDSSGKKSK DKEQIKEKFR 550
KFNEGFEDLV SKTKQYKLSD PSLKVTLKSE IISLVMPMYE RFYSRYKDSF 600
KNPRKHIKYT PDELTTVLNQ LVR 623
Length:623
Mass (Da):71,294
Last modified:February 1, 1991 - v1
Checksum:i277D89176FC2837D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti497 – 4971A → S in AAU09750. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y08787 Genomic DNA. Translation: CAA70039.1.
Y00473 Genomic DNA. Translation: CAA68535.1.
X83502 Genomic DNA. Translation: CAA58485.1.
Z49362 Genomic DNA. Translation: CAA89380.1.
AY723833 Genomic DNA. Translation: AAU09750.1.
J03546 Genomic DNA. Translation: AAA66919.1.
BK006943 Genomic DNA. Translation: DAA08714.1.
PIRiA27300.
RefSeqiNP_012450.1. NM_001181518.1.

Genome annotation databases

EnsemblFungiiYJL085W; YJL085W; YJL085W.
GeneIDi853359.
KEGGisce:YJL085W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y08787 Genomic DNA. Translation: CAA70039.1 .
Y00473 Genomic DNA. Translation: CAA68535.1 .
X83502 Genomic DNA. Translation: CAA58485.1 .
Z49362 Genomic DNA. Translation: CAA89380.1 .
AY723833 Genomic DNA. Translation: AAU09750.1 .
J03546 Genomic DNA. Translation: AAA66919.1 .
BK006943 Genomic DNA. Translation: DAA08714.1 .
PIRi A27300.
RefSeqi NP_012450.1. NM_001181518.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2B1E X-ray 2.00 A 63-623 [» ]
2B7M X-ray 3.50 A/B/C/D 63-623 [» ]
2PFV X-ray 2.10 A 63-623 [» ]
ProteinModelPortali P19658.
SMRi P19658. Positions 67-623.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33671. 87 interactions.
DIPi DIP-2934N.
IntActi P19658. 9 interactions.
MINTi MINT-700581.
STRINGi 4932.YJL085W.

Proteomic databases

MaxQBi P19658.
PaxDbi P19658.
PeptideAtlasi P19658.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL085W ; YJL085W ; YJL085W .
GeneIDi 853359.
KEGGi sce:YJL085W.

Organism-specific databases

CYGDi YJL085w.
SGDi S000003621. EXO70.

Phylogenomic databases

eggNOGi NOG245075.
HOGENOMi HOG000000807.
KOi K07195.
OMAi PRKHIKY.
OrthoDBi EOG790G8M.

Enzyme and pathway databases

BioCyci YEAST:G3O-31542-MONOMER.

Miscellaneous databases

EvolutionaryTracei P19658.
NextBioi 973780.
PROi P19658.

Gene expression databases

Genevestigatori P19658.

Family and domain databases

InterProi IPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view ]
PANTHERi PTHR12542. PTHR12542. 1 hit.
Pfami PF03081. Exo70. 1 hit.
[Graphical view ]
SUPFAMi SSF74788. SSF74788. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae."
    TerBush D.R., Maurice T., Roth D., Novick P.
    EMBO J. 15:6483-6494(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-52, IDENTIFICATION IN THE EXOCYST COMPLEX.
  2. "Nucleotide sequence of ORF2: an open reading frame upstream of the tRNA ligase gene."
    Komatsoulis G.A., Westaway S.K., Abelson J.
    Nucleic Acids Res. 15:9079-9079(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
    Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
    Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Structure and function of the yeast tRNA ligase gene."
    Westaway S.K., Phizicky E.M., Abelson J.
    J. Biol. Chem. 263:3171-3176(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70.
    Strain: EJ101.
  8. "Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70."
    Robinson N.G.G., Guo L., Imai J., Toh-e A., Matsui Y., Tamanoi F.
    Mol. Cell. Biol. 19:3580-3587(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RHO3.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p."
    Wiederkehr A., De Craene J.-O., Ferro-Novick S., Novick P.
    J. Cell Biol. 167:875-887(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p."
    Boyd C., Hughes T., Pypaert M., Novick P.
    J. Cell Biol. 167:889-901(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  13. "The critical role of EXO84P in the organization and polarized localization of the exocyst complex."
    Zhang X., Zajac A., Zhang J., Wang P., Li M., Murray J., TerBush D.R., Guo W.
    J. Biol. Chem. 280:20356-20364(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  14. "The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif."
    Dong G., Hutagalung A.H., Fu C., Novick P., Reinisch K.M.
    Nat. Struct. Mol. Biol. 12:1094-1100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 63-623, INTERACTION WITH RHO3.

Entry informationi

Entry nameiEXO70_YEAST
AccessioniPrimary (citable) accession number: P19658
Secondary accession number(s): D6VW98, E9P961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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