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P19658

- EXO70_YEAST

UniProt

P19658 - EXO70_YEAST

Protein

Exocyst complex component EXO70

Gene

EXO70

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in the assembly of the exocyst.1 Publication

    GO - Molecular functioni

    1. GTP-Rho binding Source: SGD
    2. phosphatidylinositol-4,5-bisphosphate binding Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. exocyst assembly Source: SGD
    2. exocyst localization Source: SGD
    3. exocytosis Source: SGD
    4. Golgi to plasma membrane transport Source: SGD
    5. protein transport Source: UniProtKB-KW
    6. Rho protein signal transduction Source: SGD
    7. vesicle tethering involved in exocytosis Source: SGD

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31542-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exocyst complex component EXO70
    Alternative name(s):
    Exocyst complex protein of 70 kDa
    Gene namesi
    Name:EXO70
    Ordered Locus Names:YJL085W
    ORF Names:J0932
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL085w.
    SGDiS000003621. EXO70.

    Subcellular locationi

    Bud. Bud neck
    Note: Its polarization in the cell depends on EXO84.

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. exocyst Source: SGD
    4. incipient cellular bud site Source: SGD
    5. plasma membrane Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 623623Exocyst complex component EXO70PRO_0000118975Add
    BLAST

    Proteomic databases

    MaxQBiP19658.
    PaxDbiP19658.
    PeptideAtlasiP19658.

    Expressioni

    Gene expression databases

    GenevestigatoriP19658.

    Interactioni

    Subunit structurei

    The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Interacts with RHO3.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RHO3Q002453EBI-6717,EBI-15138

    Protein-protein interaction databases

    BioGridi33671. 87 interactions.
    DIPiDIP-2934N.
    IntActiP19658. 9 interactions.
    MINTiMINT-700581.
    STRINGi4932.YJL085W.

    Structurei

    Secondary structure

    1
    623
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi69 – 713
    Helixi74 – 8916
    Helixi92 – 954
    Helixi97 – 11519
    Beta strandi116 – 1194
    Turni122 – 1243
    Helixi125 – 15329
    Helixi161 – 1677
    Helixi176 – 19116
    Helixi195 – 21420
    Turni215 – 2173
    Helixi218 – 2214
    Helixi239 – 26022
    Helixi268 – 29225
    Helixi298 – 3014
    Helixi304 – 31815
    Turni319 – 3213
    Helixi327 – 35832
    Helixi370 – 38213
    Helixi383 – 3853
    Helixi386 – 3927
    Turni393 – 3953
    Helixi398 – 4003
    Helixi408 – 4103
    Helixi423 – 45028
    Helixi469 – 48820
    Helixi492 – 4954
    Turni496 – 4983
    Helixi499 – 53234
    Beta strandi535 – 5373
    Helixi542 – 56322
    Helixi571 – 59626
    Helixi597 – 5993
    Beta strandi600 – 6023
    Helixi603 – 6053
    Helixi611 – 62212

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2B1EX-ray2.00A63-623[»]
    2B7MX-ray3.50A/B/C/D63-623[»]
    2PFVX-ray2.10A63-623[»]
    ProteinModelPortaliP19658.
    SMRiP19658. Positions 67-623.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP19658.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the EXO70 family.Curated

    Phylogenomic databases

    eggNOGiNOG245075.
    HOGENOMiHOG000000807.
    KOiK07195.
    OMAiPRKHIKY.
    OrthoDBiEOG790G8M.

    Family and domain databases

    InterProiIPR016159. Cullin_repeat-like_dom.
    IPR004140. Exo70.
    [Graphical view]
    PANTHERiPTHR12542. PTHR12542. 1 hit.
    PfamiPF03081. Exo70. 1 hit.
    [Graphical view]
    SUPFAMiSSF74788. SSF74788. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P19658-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAEIDIDEA DVLVLSQELQ KTSKLTFEIN KSLKKIAATS NQSSQLFTPI    50
    LARNNVLTTL QRNIESTLNS VASVKDLANE ASKYEIILQK GINQVGLKQY 100
    TQVVHKLDDM LEDIQSGQAN REENSEFHGI LTHLEQLIKR SEAQLRVYFI 150
    SILNSIKPFD PQINITKKMP FPYYEDQQLG ALSWILDYFH GNSEGSIIQD 200
    ILVGERSKLI LKCMAFLEPF AKEISTAKNA PYEKGSSGMN SYTEALLGFI 250
    ANEKSLVDDL YSQYTESKPH VLSQILSPLI SAYAKLFGAN LKIVRSNLEN 300
    FGFFSFELVE SINDVKKSLR GKELQNYNLL QDCTQEVRQV TQSLFRDAID 350
    RIIKKANSIS TIPSNNGVTE ATVDTMSRLR KFSEYKNGCL GAMDNITREN 400
    WLPSNYKEKE YTLQNEALNW EDHNVLLSCF ISDCIDTLAV NLERKAQIAL 450
    MPNQEPDVAN PNSSKNKHKQ RIGFFILMNL TLVEQIVEKS ELNLMLAGEG 500
    HSRLERLKKR YISYMVSDWR DLTANLMDSV FIDSSGKKSK DKEQIKEKFR 550
    KFNEGFEDLV SKTKQYKLSD PSLKVTLKSE IISLVMPMYE RFYSRYKDSF 600
    KNPRKHIKYT PDELTTVLNQ LVR 623
    Length:623
    Mass (Da):71,294
    Last modified:February 1, 1991 - v1
    Checksum:i277D89176FC2837D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti497 – 4971A → S in AAU09750. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y08787 Genomic DNA. Translation: CAA70039.1.
    Y00473 Genomic DNA. Translation: CAA68535.1.
    X83502 Genomic DNA. Translation: CAA58485.1.
    Z49362 Genomic DNA. Translation: CAA89380.1.
    AY723833 Genomic DNA. Translation: AAU09750.1.
    J03546 Genomic DNA. Translation: AAA66919.1.
    BK006943 Genomic DNA. Translation: DAA08714.1.
    PIRiA27300.
    RefSeqiNP_012450.1. NM_001181518.1.

    Genome annotation databases

    EnsemblFungiiYJL085W; YJL085W; YJL085W.
    GeneIDi853359.
    KEGGisce:YJL085W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y08787 Genomic DNA. Translation: CAA70039.1 .
    Y00473 Genomic DNA. Translation: CAA68535.1 .
    X83502 Genomic DNA. Translation: CAA58485.1 .
    Z49362 Genomic DNA. Translation: CAA89380.1 .
    AY723833 Genomic DNA. Translation: AAU09750.1 .
    J03546 Genomic DNA. Translation: AAA66919.1 .
    BK006943 Genomic DNA. Translation: DAA08714.1 .
    PIRi A27300.
    RefSeqi NP_012450.1. NM_001181518.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2B1E X-ray 2.00 A 63-623 [» ]
    2B7M X-ray 3.50 A/B/C/D 63-623 [» ]
    2PFV X-ray 2.10 A 63-623 [» ]
    ProteinModelPortali P19658.
    SMRi P19658. Positions 67-623.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33671. 87 interactions.
    DIPi DIP-2934N.
    IntActi P19658. 9 interactions.
    MINTi MINT-700581.
    STRINGi 4932.YJL085W.

    Proteomic databases

    MaxQBi P19658.
    PaxDbi P19658.
    PeptideAtlasi P19658.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL085W ; YJL085W ; YJL085W .
    GeneIDi 853359.
    KEGGi sce:YJL085W.

    Organism-specific databases

    CYGDi YJL085w.
    SGDi S000003621. EXO70.

    Phylogenomic databases

    eggNOGi NOG245075.
    HOGENOMi HOG000000807.
    KOi K07195.
    OMAi PRKHIKY.
    OrthoDBi EOG790G8M.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31542-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P19658.
    NextBioi 973780.
    PROi P19658.

    Gene expression databases

    Genevestigatori P19658.

    Family and domain databases

    InterProi IPR016159. Cullin_repeat-like_dom.
    IPR004140. Exo70.
    [Graphical view ]
    PANTHERi PTHR12542. PTHR12542. 1 hit.
    Pfami PF03081. Exo70. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74788. SSF74788. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae."
      TerBush D.R., Maurice T., Roth D., Novick P.
      EMBO J. 15:6483-6494(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-52, IDENTIFICATION IN THE EXOCYST COMPLEX.
    2. "Nucleotide sequence of ORF2: an open reading frame upstream of the tRNA ligase gene."
      Komatsoulis G.A., Westaway S.K., Abelson J.
      Nucleic Acids Res. 15:9079-9079(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site."
      Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K.
      Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. "Structure and function of the yeast tRNA ligase gene."
      Westaway S.K., Phizicky E.M., Abelson J.
      J. Biol. Chem. 263:3171-3176(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70.
      Strain: EJ101.
    8. "Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70."
      Robinson N.G.G., Guo L., Imai J., Toh-e A., Matsui Y., Tamanoi F.
      Mol. Cell. Biol. 19:3580-3587(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RHO3.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p."
      Wiederkehr A., De Craene J.-O., Ferro-Novick S., Novick P.
      J. Cell Biol. 167:875-887(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p."
      Boyd C., Hughes T., Pypaert M., Novick P.
      J. Cell Biol. 167:889-901(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    13. "The critical role of EXO84P in the organization and polarized localization of the exocyst complex."
      Zhang X., Zajac A., Zhang J., Wang P., Li M., Murray J., TerBush D.R., Guo W.
      J. Biol. Chem. 280:20356-20364(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    14. "The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif."
      Dong G., Hutagalung A.H., Fu C., Novick P., Reinisch K.M.
      Nat. Struct. Mol. Biol. 12:1094-1100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 63-623, INTERACTION WITH RHO3.

    Entry informationi

    Entry nameiEXO70_YEAST
    AccessioniPrimary (citable) accession number: P19658
    Secondary accession number(s): D6VW98, E9P961
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4280 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3