Exocyst complex component EXO70 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P19658 (EXO70_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Exocyst complex component EXO70

Alternative name(s):
Exocyst complex protein of 70 kDa

Gene names

Name:	EXO70
Ordered Locus Names:	YJL085W
ORF Names:	J0932

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in the assembly of the exocyst. Ref.11
Subunit structure	The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84. Interacts with RHO3. Ref.1 Ref.8 Ref.14
Subcellular location	Bud. Bud neck. Note: Its polarization in the cell depends on EXO84. Ref.9 Ref.12 Ref.13
Miscellaneous	Present with 4280 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the EXO70 family.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to plasma membrane transport Inferred from mutant phenotype PubMed 17339375. Source: SGD Rho protein signal transduction Inferred from direct assay PubMed 19955214. Source: SGD exocyst assembly Inferred from mutant phenotype Ref.11 PubMed 18946089. Source: SGD exocyst localization Inferred from genetic interaction PubMed 17717527 PubMed 18946089. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle tethering involved in exocytosis Inferred by curator Ref.1. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay PubMed 18946089 PubMed 22842922. Source: SGD cellular bud tip Inferred from direct assay Ref.8 PubMed 17717527 PubMed 18946089 PubMed 22842922. Source: SGD exocyst Inferred from direct assay Ref.1. Source: SGD incipient cellular bud site Inferred from direct assay PubMed 18195105. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD plasma membrane Inferred from direct assay PubMed 17717527. Source: SGD
Molecular_function	GTP-Rho binding Inferred from direct assay PubMed 10588647. Source: SGD phosphatidylinositol-4,5-bisphosphate binding Inferred from direct assay PubMed 17717527. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 623

623

Exocyst complex component EXO70

PRO_0000118975

Experimental info

Sequence conflict

497

A → S in AAU09750. Ref.6

Secondary structure

623

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P19658 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 277D89176FC2837D
Show »

FASTA

623

71,294

        10         20         30         40         50         60 
MPAEIDIDEA DVLVLSQELQ KTSKLTFEIN KSLKKIAATS NQSSQLFTPI LARNNVLTTL 

        70         80         90        100        110        120 
QRNIESTLNS VASVKDLANE ASKYEIILQK GINQVGLKQY TQVVHKLDDM LEDIQSGQAN 

       130        140        150        160        170        180 
REENSEFHGI LTHLEQLIKR SEAQLRVYFI SILNSIKPFD PQINITKKMP FPYYEDQQLG 

       190        200        210        220        230        240 
ALSWILDYFH GNSEGSIIQD ILVGERSKLI LKCMAFLEPF AKEISTAKNA PYEKGSSGMN 

       250        260        270        280        290        300 
SYTEALLGFI ANEKSLVDDL YSQYTESKPH VLSQILSPLI SAYAKLFGAN LKIVRSNLEN 

       310        320        330        340        350        360 
FGFFSFELVE SINDVKKSLR GKELQNYNLL QDCTQEVRQV TQSLFRDAID RIIKKANSIS 

       370        380        390        400        410        420 
TIPSNNGVTE ATVDTMSRLR KFSEYKNGCL GAMDNITREN WLPSNYKEKE YTLQNEALNW 

       430        440        450        460        470        480 
EDHNVLLSCF ISDCIDTLAV NLERKAQIAL MPNQEPDVAN PNSSKNKHKQ RIGFFILMNL 

       490        500        510        520        530        540 
TLVEQIVEKS ELNLMLAGEG HSRLERLKKR YISYMVSDWR DLTANLMDSV FIDSSGKKSK 

       550        560        570        580        590        600 
DKEQIKEKFR KFNEGFEDLV SKTKQYKLSD PSLKVTLKSE IISLVMPMYE RFYSRYKDSF 

       610        620 
KNPRKHIKYT PDELTTVLNQ LVR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae." TerBush D.R., Maurice T., Roth D., Novick P. EMBO J. 15:6483-6494(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-52, IDENTIFICATION IN THE EXOCYST COMPLEX.
[2]	"Nucleotide sequence of ORF2: an open reading frame upstream of the tRNA ligase gene." Komatsoulis G.A., Westaway S.K., Abelson J. Nucleic Acids Res. 15:9079-9079(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site." Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K. Yeast 11:681-689(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Structure and function of the yeast tRNA ligase gene." Westaway S.K., Phizicky E.M., Abelson J. J. Biol. Chem. 263:3171-3176(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70. Strain: EJ101.
[8]	"Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70." Robinson N.G.G., Guo L., Imai J., Toh-e A., Matsui Y., Tamanoi F. Mol. Cell. Biol. 19:3580-3587(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RHO3.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p." Wiederkehr A., De Craene J.-O., Ferro-Novick S., Novick P. J. Cell Biol. 167:875-887(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p." Boyd C., Hughes T., Pypaert M., Novick P. J. Cell Biol. 167:889-901(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[13]	"The critical role of EXO84P in the organization and polarized localization of the exocyst complex." Zhang X., Zajac A., Zhang J., Wang P., Li M., Murray J., TerBush D.R., Guo W. J. Biol. Chem. 280:20356-20364(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[14]	"The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif." Dong G., Hutagalung A.H., Fu C., Novick P., Reinisch K.M. Nat. Struct. Mol. Biol. 12:1094-1100(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 63-623, INTERACTION WITH RHO3.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y08787 Genomic DNA. Translation: CAA70039.1.
Y00473 Genomic DNA. Translation: CAA68535.1.
X83502 Genomic DNA. Translation: CAA58485.1.
Z49362 Genomic DNA. Translation: CAA89380.1.
AY723833 Genomic DNA. Translation: AAU09750.1.
J03546 Genomic DNA. Translation: AAA66919.1.
BK006943 Genomic DNA. Translation: DAA08714.1.

PIR

A27300.

RefSeq

NP_012450.1. NM_001181518.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2B1E	X-ray	2.00	A	63-623	[»]
2B7M	X-ray	3.50	A/B/C/D	63-623	[»]
2PFV	X-ray	2.10	A	63-623	[»]

ProteinModelPortal

P19658.

SMR

P19658. Positions 67-623.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2934N.

IntAct

P19658. 8 interactions.

MINT

MINT-700581.

STRING

4932.YJL085W.

Proteomic databases

PaxDb

P19658.

PeptideAtlas

P19658.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJL085W; YJL085W; YJL085W.

GeneID

853359.

KEGG

sce:YJL085W.

Organism-specific databases

CYGD

YJL085w.

SGD

S000003621. EXO70.

Phylogenomic databases

eggNOG

NOG245075.

GeneTree

ENSGT00390000003595.

HOGENOM

HOG000000807.

K07195.

OMA

PRKHIKY.

OrthoDB

EOG45MRDR.

Gene expression databases

Genevestigator

P19658.

GermOnline

YJL085W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR016159. Cullin_repeat-like_dom.
IPR004140. Exo70.
[Graphical view]

PANTHER

PTHR12542. PTHR12542. 1 hit.

Pfam

PF03081. Exo70. 1 hit.
[Graphical view]

SUPFAM

SSF74788. Cullin_repeat-like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P19658.

NextBio

973780.

Entry information

Entry name

EXO70_YEAST

Accession

Primary (citable) accession number: P19658
Secondary accession number(s): D6VW98, E9P961

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents