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Protein

Plasma membrane ATPase 2

Gene

PMA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4074-aspartylphosphate intermediateBy similarity1
Metal bindingi663MagnesiumBy similarity1
Metal bindingi667MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • ATP biosynthetic process Source: InterPro
  • proton transport Source: SGD
  • regulation of intracellular pH Source: GO_Central
  • regulation of pH Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33950-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane ATPase 2 (EC:3.6.3.6)
Alternative name(s):
Proton pump 2
Gene namesi
Name:PMA2
Ordered Locus Names:YPL036W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL036W.
SGDiS000005957. PMA2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 144CytoplasmicSequence analysisAdd BLAST144
Transmembranei145 – 165Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini166 – 169ExtracellularSequence analysis4
Transmembranei170 – 189Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini190 – 320CytoplasmicSequence analysisAdd BLAST131
Transmembranei321 – 342Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini343 – 353ExtracellularSequence analysisAdd BLAST11
Transmembranei354 – 376Helical; Name=4Sequence analysisAdd BLAST23
Topological domaini377 – 748CytoplasmicSequence analysisAdd BLAST372
Transmembranei749 – 767Helical; Name=5Sequence analysisAdd BLAST19
Topological domaini768 – 783ExtracellularSequence analysisAdd BLAST16
Transmembranei784 – 803Helical; Name=6Sequence analysisAdd BLAST20
Topological domaini804 – 853CytoplasmicSequence analysisAdd BLAST50
Transmembranei854 – 874Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini875 – 886ExtracellularSequence analysisAdd BLAST12
Transmembranei887 – 903Helical; Name=8Sequence analysisAdd BLAST17
Topological domaini904 – 947CytoplasmicSequence analysisAdd BLAST44

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462721 – 947Plasma membrane ATPase 2Add BLAST947

Proteomic databases

MaxQBiP19657.
PRIDEiP19657.

PTM databases

iPTMnetiP19657.

Interactioni

Protein-protein interaction databases

BioGridi36143. 100 interactors.
DIPiDIP-4036N.
IntActiP19657. 1 interactor.
MINTiMINT-485284.

Structurei

3D structure databases

ProteinModelPortaliP19657.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi33 – 47Poly-SerAdd BLAST15
Compositional biasi62 – 91Asp/Glu-rich (acidic)Add BLAST30

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00840000130975.
HOGENOMiHOG000160005.
InParanoidiP19657.
KOiK01535.
OMAiDGHAFEE.
OrthoDBiEOG092C0HLD.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTEAKQYK EKPSKEYLHA SDGDDPANNS AASSSSSSST STSASSSAAA
60 70 80 90 100
VPRKAAAASA ADDSDSDEDI DQLIDELQSN YGEGDESGEE EVRTDGVHAG
110 120 130 140 150
QRVVPEKDLS TDPAYGLTSD EVARRRKKYG LNQMAEENES LIVKFLMFFV
160 170 180 190 200
GPIQFVMEAA AILAAGLSDW VDVGVICALL LLNASVGFIQ EFQAGSIVDE
210 220 230 240 250
LKKTLANTAT VIRDGQLIEI PANEVVPGEI LQLESGTIAP ADGRIVTEDC
260 270 280 290 300
FLQIDQSAIT GESLAAEKHY GDEVFSSSTV KTGEAFMVVT ATGDNTFVGR
310 320 330 340 350
AAALVGQASG VEGHFTEVLN GIGIILLVLV IATLLLVWTA CFYRTVGIVS
360 370 380 390 400
ILRYTLGITI IGVPVGLPAV VTTTMAVGAA YLAKKQAIVQ KLSAIESLAG
410 420 430 440 450
VEILCSDKTG TLTKNKLSLH EPYTVEGVSP DDLMLTACLA ASRKKKGLDA
460 470 480 490 500
IDKAFLKSLI EYPKAKDALT KYKVLEFHPF DPVSKKVTAV VESPEGERIV
510 520 530 540 550
CVKGAPLFVL KTVEEDHPIP EDVHENYENK VAELASRGFR ALGVARKRGE
560 570 580 590 600
GHWEILGVMP CMDPPRDDTA QTINEARNLG LRIKMLTGDA VGIAKETCRQ
610 620 630 640 650
LGLGTNIYNA ERLGLGGGGD MPGSELADFV ENADGFAEVF PQHKYRVVEI
660 670 680 690 700
LQNRGYLVAM TGDGVNDAPS LKKADTGIAV EGATDAARSA ADIVFLAPGL
710 720 730 740 750
SAIIDALKTS RQIFHRMYSY VVYRIALSLH LEIFLGLWIA ILNNSLDINL
760 770 780 790 800
IVFIAIFADV ATLTIAYDNA PYAPEPVKWN LPRLWGMSII LGIVLAIGSW
810 820 830 840 850
ITLTTMFLPN GGIIQNFGAM NGVMFLQISL TENWLIFVTR AAGPFWSSIP
860 870 880 890 900
SWQLAGAVFA VDIIATMFTL FGWWSENWTD IVSVVRVWIW SIGIFCVLGG
910 920 930 940
FYYIMSTSQA FDRLMNGKSL KEKKSTRSVE DFMAAMQRVS TQHEKSS
Length:947
Mass (Da):102,172
Last modified:October 1, 1996 - v3
Checksum:i24CF0D3EAFD1E9D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti944E → D in AAA83387 (PubMed:2904437).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04421 Genomic DNA. Translation: AAA83387.1.
U44030 Genomic DNA. Translation: AAB68184.1.
BK006949 Genomic DNA. Translation: DAA11394.1.
PIRiS62039. PXBY2P.
RefSeqiNP_015289.1. NM_001183850.1.

Genome annotation databases

EnsemblFungiiYPL036W; YPL036W; YPL036W.
GeneIDi856071.
KEGGisce:YPL036W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04421 Genomic DNA. Translation: AAA83387.1.
U44030 Genomic DNA. Translation: AAB68184.1.
BK006949 Genomic DNA. Translation: DAA11394.1.
PIRiS62039. PXBY2P.
RefSeqiNP_015289.1. NM_001183850.1.

3D structure databases

ProteinModelPortaliP19657.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36143. 100 interactors.
DIPiDIP-4036N.
IntActiP19657. 1 interactor.
MINTiMINT-485284.

PTM databases

iPTMnetiP19657.

Proteomic databases

MaxQBiP19657.
PRIDEiP19657.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL036W; YPL036W; YPL036W.
GeneIDi856071.
KEGGisce:YPL036W.

Organism-specific databases

EuPathDBiFungiDB:YPL036W.
SGDiS000005957. PMA2.

Phylogenomic databases

GeneTreeiENSGT00840000130975.
HOGENOMiHOG000160005.
InParanoidiP19657.
KOiK01535.
OMAiDGHAFEE.
OrthoDBiEOG092C0HLD.

Enzyme and pathway databases

BioCyciYEAST:G3O-33950-MONOMER.

Miscellaneous databases

PROiP19657.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMA2_YEAST
AccessioniPrimary (citable) accession number: P19657
Secondary accession number(s): D6W3X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 161 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two plasma membrane ATPases in yeast. This is the minor isoform.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.