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P19657

- PMA2_YEAST

UniProt

P19657 - PMA2_YEAST

Protein

Plasma membrane ATPase 2

Gene

PMA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 3 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

    Catalytic activityi

    ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei407 – 40714-aspartylphosphate intermediateBy similarity
    Metal bindingi663 – 6631MagnesiumBy similarity
    Metal bindingi667 – 6671MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrogen-exporting ATPase activity, phosphorylative mechanism Source: SGD
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP biosynthetic process Source: InterPro
    2. hydrogen ion transmembrane transport Source: GOC
    3. proton transport Source: SGD
    4. regulation of pH Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Hydrogen ion transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33950-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plasma membrane ATPase 2 (EC:3.6.3.6)
    Alternative name(s):
    Proton pump 2
    Gene namesi
    Name:PMA2
    Ordered Locus Names:YPL036W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL036w.
    SGDiS000005957. PMA2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 947947Plasma membrane ATPase 2PRO_0000046272Add
    BLAST

    Proteomic databases

    PaxDbiP19657.
    PeptideAtlasiP19657.
    PRIDEiP19657.

    Expressioni

    Gene expression databases

    GenevestigatoriP19657.

    Interactioni

    Protein-protein interaction databases

    BioGridi36143. 100 interactions.
    DIPiDIP-4036N.
    IntActiP19657. 1 interaction.
    MINTiMINT-485284.
    STRINGi4932.YPL036W.

    Structurei

    3D structure databases

    ProteinModelPortaliP19657.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 144144CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini166 – 1694ExtracellularSequence Analysis
    Topological domaini190 – 320131CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini343 – 35311ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini377 – 748372CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini768 – 78316ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini804 – 85350CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini875 – 88612ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini904 – 94744CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei145 – 16521Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei170 – 18920Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei321 – 34222Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei354 – 37623Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei749 – 76719Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei784 – 80320Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei854 – 87421Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei887 – 90317Helical; Name=8Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi33 – 4715Poly-SerAdd
    BLAST
    Compositional biasi62 – 9130Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00730000113900.
    HOGENOMiHOG000160005.
    KOiK01535.
    OMAiVGFWEEY.
    OrthoDBiEOG789CKN.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 2 hits.
    InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR006534. H+_ATPase_P-typ_IIIA.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    PR00120. HATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19657-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSTEAKQYK EKPSKEYLHA SDGDDPANNS AASSSSSSST STSASSSAAA    50
    VPRKAAAASA ADDSDSDEDI DQLIDELQSN YGEGDESGEE EVRTDGVHAG 100
    QRVVPEKDLS TDPAYGLTSD EVARRRKKYG LNQMAEENES LIVKFLMFFV 150
    GPIQFVMEAA AILAAGLSDW VDVGVICALL LLNASVGFIQ EFQAGSIVDE 200
    LKKTLANTAT VIRDGQLIEI PANEVVPGEI LQLESGTIAP ADGRIVTEDC 250
    FLQIDQSAIT GESLAAEKHY GDEVFSSSTV KTGEAFMVVT ATGDNTFVGR 300
    AAALVGQASG VEGHFTEVLN GIGIILLVLV IATLLLVWTA CFYRTVGIVS 350
    ILRYTLGITI IGVPVGLPAV VTTTMAVGAA YLAKKQAIVQ KLSAIESLAG 400
    VEILCSDKTG TLTKNKLSLH EPYTVEGVSP DDLMLTACLA ASRKKKGLDA 450
    IDKAFLKSLI EYPKAKDALT KYKVLEFHPF DPVSKKVTAV VESPEGERIV 500
    CVKGAPLFVL KTVEEDHPIP EDVHENYENK VAELASRGFR ALGVARKRGE 550
    GHWEILGVMP CMDPPRDDTA QTINEARNLG LRIKMLTGDA VGIAKETCRQ 600
    LGLGTNIYNA ERLGLGGGGD MPGSELADFV ENADGFAEVF PQHKYRVVEI 650
    LQNRGYLVAM TGDGVNDAPS LKKADTGIAV EGATDAARSA ADIVFLAPGL 700
    SAIIDALKTS RQIFHRMYSY VVYRIALSLH LEIFLGLWIA ILNNSLDINL 750
    IVFIAIFADV ATLTIAYDNA PYAPEPVKWN LPRLWGMSII LGIVLAIGSW 800
    ITLTTMFLPN GGIIQNFGAM NGVMFLQISL TENWLIFVTR AAGPFWSSIP 850
    SWQLAGAVFA VDIIATMFTL FGWWSENWTD IVSVVRVWIW SIGIFCVLGG 900
    FYYIMSTSQA FDRLMNGKSL KEKKSTRSVE DFMAAMQRVS TQHEKSS 947
    Length:947
    Mass (Da):102,172
    Last modified:October 1, 1996 - v3
    Checksum:i24CF0D3EAFD1E9D5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti944 – 9441E → D in AAA83387. (PubMed:2904437)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04421 Genomic DNA. Translation: AAA83387.1.
    U44030 Genomic DNA. Translation: AAB68184.1.
    BK006949 Genomic DNA. Translation: DAA11394.1.
    PIRiS62039. PXBY2P.
    RefSeqiNP_015289.1. NM_001183850.1.

    Genome annotation databases

    EnsemblFungiiYPL036W; YPL036W; YPL036W.
    GeneIDi856071.
    KEGGisce:YPL036W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04421 Genomic DNA. Translation: AAA83387.1 .
    U44030 Genomic DNA. Translation: AAB68184.1 .
    BK006949 Genomic DNA. Translation: DAA11394.1 .
    PIRi S62039. PXBY2P.
    RefSeqi NP_015289.1. NM_001183850.1.

    3D structure databases

    ProteinModelPortali P19657.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36143. 100 interactions.
    DIPi DIP-4036N.
    IntActi P19657. 1 interaction.
    MINTi MINT-485284.
    STRINGi 4932.YPL036W.

    Proteomic databases

    PaxDbi P19657.
    PeptideAtlasi P19657.
    PRIDEi P19657.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL036W ; YPL036W ; YPL036W .
    GeneIDi 856071.
    KEGGi sce:YPL036W.

    Organism-specific databases

    CYGDi YPL036w.
    SGDi S000005957. PMA2.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00730000113900.
    HOGENOMi HOG000160005.
    KOi K01535.
    OMAi VGFWEEY.
    OrthoDBi EOG789CKN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33950-MONOMER.

    Miscellaneous databases

    NextBioi 981065.

    Gene expression databases

    Genevestigatori P19657.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 2 hits.
    InterProi IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR006534. H+_ATPase_P-typ_IIIA.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    PR00120. HATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    TIGRFAMsi TIGR01647. ATPase-IIIA_H. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A second transport ATPase gene in Saccharomyces cerevisiae."
      Schlesser A., Ulaszewski S., Ghislain M., Goffeau A.
      J. Biol. Chem. 263:19480-19487(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiPMA2_YEAST
    AccessioniPrimary (citable) accession number: P19657
    Secondary accession number(s): D6W3X8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 141 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are two plasma membrane ATPases in yeast. This is the minor isoform.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3