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P19657

- PMA2_YEAST

UniProt

P19657 - PMA2_YEAST

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Protein

Plasma membrane ATPase 2

Gene

PMA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei407 – 40714-aspartylphosphate intermediateBy similarity
Metal bindingi663 – 6631MagnesiumBy similarity
Metal bindingi667 – 6671MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrogen-exporting ATPase activity, phosphorylative mechanism Source: SGD
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP biosynthetic process Source: InterPro
  2. hydrogen ion transmembrane transport Source: GOC
  3. proton transport Source: SGD
  4. regulation of pH Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33950-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane ATPase 2 (EC:3.6.3.6)
Alternative name(s):
Proton pump 2
Gene namesi
Name:PMA2
Ordered Locus Names:YPL036W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL036w.
SGDiS000005957. PMA2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 144144CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei145 – 16521Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini166 – 1694ExtracellularSequence Analysis
Transmembranei170 – 18920Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini190 – 320131CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei321 – 34222Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini343 – 35311ExtracellularSequence AnalysisAdd
BLAST
Transmembranei354 – 37623Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini377 – 748372CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei749 – 76719Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini768 – 78316ExtracellularSequence AnalysisAdd
BLAST
Transmembranei784 – 80320Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini804 – 85350CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei854 – 87421Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini875 – 88612ExtracellularSequence AnalysisAdd
BLAST
Transmembranei887 – 90317Helical; Name=8Sequence AnalysisAdd
BLAST
Topological domaini904 – 94744CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 947947Plasma membrane ATPase 2PRO_0000046272Add
BLAST

Proteomic databases

PaxDbiP19657.
PeptideAtlasiP19657.
PRIDEiP19657.

Expressioni

Gene expression databases

GenevestigatoriP19657.

Interactioni

Protein-protein interaction databases

BioGridi36143. 100 interactions.
DIPiDIP-4036N.
IntActiP19657. 1 interaction.
MINTiMINT-485284.
STRINGi4932.YPL036W.

Structurei

3D structure databases

ProteinModelPortaliP19657.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi33 – 4715Poly-SerAdd
BLAST
Compositional biasi62 – 9130Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00760000119767.
HOGENOMiHOG000160005.
InParanoidiP19657.
KOiK01535.
OMAiVGFWEEY.
OrthoDBiEOG789CKN.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR006534. H+_ATPase_P-typ_IIIA.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19657-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSTEAKQYK EKPSKEYLHA SDGDDPANNS AASSSSSSST STSASSSAAA
60 70 80 90 100
VPRKAAAASA ADDSDSDEDI DQLIDELQSN YGEGDESGEE EVRTDGVHAG
110 120 130 140 150
QRVVPEKDLS TDPAYGLTSD EVARRRKKYG LNQMAEENES LIVKFLMFFV
160 170 180 190 200
GPIQFVMEAA AILAAGLSDW VDVGVICALL LLNASVGFIQ EFQAGSIVDE
210 220 230 240 250
LKKTLANTAT VIRDGQLIEI PANEVVPGEI LQLESGTIAP ADGRIVTEDC
260 270 280 290 300
FLQIDQSAIT GESLAAEKHY GDEVFSSSTV KTGEAFMVVT ATGDNTFVGR
310 320 330 340 350
AAALVGQASG VEGHFTEVLN GIGIILLVLV IATLLLVWTA CFYRTVGIVS
360 370 380 390 400
ILRYTLGITI IGVPVGLPAV VTTTMAVGAA YLAKKQAIVQ KLSAIESLAG
410 420 430 440 450
VEILCSDKTG TLTKNKLSLH EPYTVEGVSP DDLMLTACLA ASRKKKGLDA
460 470 480 490 500
IDKAFLKSLI EYPKAKDALT KYKVLEFHPF DPVSKKVTAV VESPEGERIV
510 520 530 540 550
CVKGAPLFVL KTVEEDHPIP EDVHENYENK VAELASRGFR ALGVARKRGE
560 570 580 590 600
GHWEILGVMP CMDPPRDDTA QTINEARNLG LRIKMLTGDA VGIAKETCRQ
610 620 630 640 650
LGLGTNIYNA ERLGLGGGGD MPGSELADFV ENADGFAEVF PQHKYRVVEI
660 670 680 690 700
LQNRGYLVAM TGDGVNDAPS LKKADTGIAV EGATDAARSA ADIVFLAPGL
710 720 730 740 750
SAIIDALKTS RQIFHRMYSY VVYRIALSLH LEIFLGLWIA ILNNSLDINL
760 770 780 790 800
IVFIAIFADV ATLTIAYDNA PYAPEPVKWN LPRLWGMSII LGIVLAIGSW
810 820 830 840 850
ITLTTMFLPN GGIIQNFGAM NGVMFLQISL TENWLIFVTR AAGPFWSSIP
860 870 880 890 900
SWQLAGAVFA VDIIATMFTL FGWWSENWTD IVSVVRVWIW SIGIFCVLGG
910 920 930 940
FYYIMSTSQA FDRLMNGKSL KEKKSTRSVE DFMAAMQRVS TQHEKSS
Length:947
Mass (Da):102,172
Last modified:October 1, 1996 - v3
Checksum:i24CF0D3EAFD1E9D5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti944 – 9441E → D in AAA83387. (PubMed:2904437)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04421 Genomic DNA. Translation: AAA83387.1.
U44030 Genomic DNA. Translation: AAB68184.1.
BK006949 Genomic DNA. Translation: DAA11394.1.
PIRiS62039. PXBY2P.
RefSeqiNP_015289.1. NM_001183850.1.

Genome annotation databases

EnsemblFungiiYPL036W; YPL036W; YPL036W.
GeneIDi856071.
KEGGisce:YPL036W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04421 Genomic DNA. Translation: AAA83387.1 .
U44030 Genomic DNA. Translation: AAB68184.1 .
BK006949 Genomic DNA. Translation: DAA11394.1 .
PIRi S62039. PXBY2P.
RefSeqi NP_015289.1. NM_001183850.1.

3D structure databases

ProteinModelPortali P19657.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36143. 100 interactions.
DIPi DIP-4036N.
IntActi P19657. 1 interaction.
MINTi MINT-485284.
STRINGi 4932.YPL036W.

Proteomic databases

PaxDbi P19657.
PeptideAtlasi P19657.
PRIDEi P19657.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL036W ; YPL036W ; YPL036W .
GeneIDi 856071.
KEGGi sce:YPL036W.

Organism-specific databases

CYGDi YPL036w.
SGDi S000005957. PMA2.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00760000119767.
HOGENOMi HOG000160005.
InParanoidi P19657.
KOi K01535.
OMAi VGFWEEY.
OrthoDBi EOG789CKN.

Enzyme and pathway databases

BioCyci YEAST:G3O-33950-MONOMER.

Miscellaneous databases

NextBioi 981065.

Gene expression databases

Genevestigatori P19657.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProi IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR006534. H+_ATPase_P-typ_IIIA.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
PR00120. HATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
TIGRFAMsi TIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A second transport ATPase gene in Saccharomyces cerevisiae."
    Schlesser A., Ulaszewski S., Ghislain M., Goffeau A.
    J. Biol. Chem. 263:19480-19487(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiPMA2_YEAST
AccessioniPrimary (citable) accession number: P19657
Secondary accession number(s): D6W3X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two plasma membrane ATPases in yeast. This is the minor isoform.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3