P19656 (NLTP_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Non-specific lipid-transfer protein Short name=LTP Alternative name(s): Phospholipid transfer protein Short name=PLTP Allergen=Zea m 14
Organism	Zea mays (Maize)
Taxonomic identifier	4577 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the plant LTP family.

Ontologies

Keywords
Biological process	Transport
Coding sequence diversity	Alternative splicing
Disease	Allergen
Domain	Signal
Ligand	Lipid-binding
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid transport Inferred from electronic annotation. Source: InterPro
Molecular function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P19656-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P19656-2)

Also known as: long;

The sequence of this isoform differs from the canonical sequence as follows:
     119-120: VN → YSRRMHASAD

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Ref.1

Chain

28 – 120

93

Non-specific lipid-transfer protein

PRO_0000018389

Amino acid modifications

Disulfide bond

31 ↔ 79

By similarity

Disulfide bond

41 ↔ 56

By similarity

Disulfide bond

57 ↔ 102

By similarity

Disulfide bond

77 ↔ 116

By similarity

Natural variations

Alternative sequence

119 – 120

2

VN → YSRRMHASAD in isoform 2.

VSP_003147

Secondary structure

1

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120

Helix Strand Turn

Details...

Helix

31 – 38

8

Helix

39 – 41

3

Helix

42 – 45

4

Beta strand

49 – 51

3

Helix

54 – 66

13

Helix

70 – 85

16

Helix

92 – 101

10

Helix

116 – 118

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: 28F27EBAE3910218	FASTA	120	11,705
	10 20 30 40 50 60 MARTQQLAVV ATAVVALVLL AAATSEAAIS CGQVASAIAP CISYARGQGS GPSAGCCSGV 70 80 90 100 110 120 RSLNNAARTT ADRRAACNCL KNAAAGVSGL NAGNAASIPS KCGVSIPYTI STSTDCSRVN
	Isoform 2 (long) [UniParc]. Checksum: C9921F66B1F43762	FASTA	128	12,667
	10 20 30 40 50 60 MARTQQLAVV ATAVVALVLL AAATSEAAIS CGQVASAIAP CISYARGQGS GPSAGCCSGV 70 80 90 100 110 120 RSLNNAARTT ADRRAACNCL KNAAAGVSGL NAGNAASIPS KCGVSIPYTI STSTDCSRYS RRMHASAD

References

[1]	"Phospholipid transfer protein: full-length cDNA and amino acid sequence in maize. Amino acid sequence homologies between plant phospholipid transfer proteins." Tchang F., This P., Stiefel V., Arondel V., Morch M.-D., Pages M., Puigdomenech P., Grellet F., Delseny M., Bouillon P., Huet J.-C., Guerbette F., Beauvais-Cante F., Duranton H., Pernollet J.-C., Kader J.-C. J. Biol. Chem. 263:16849-16855(1988) [PubMed: 3182817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-120. Tissue: Seed.
[2]	"Multiple mRNA coding for phospholipid-transfer protein from Zea mays arise from alternative splicing." Arondel V., Tchang F., Baillet B., Vignols F., Grellet F., Delseny M., Kader J.-C., Puigdomenech P. Gene 99:133-136(1991) [PubMed: 2022320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings." Shin D.H., Lee J.Y., Hwang K.Y., Kim K.K., Suh S.W. Structure 3:189-199(1995) [PubMed: 7735835] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[4]	"Two-dimensional 1H-NMR studies of maize lipid-transfer protein. Sequence-specific assignment and secondary structure." Petit M.-C., Sodano P., Marion D., Ptak M. Eur. J. Biochem. 222:1047-1054(1994) [PubMed: 8026483] [Abstract] Cited for: STRUCTURE BY NMR.
[5]	"Solution structure and lipid binding of a nonspecific lipid transfer protein extracted from maize seeds." Gomar J., Petit M.-C., Sodano P., Sy D., Marion D., Kader J.-C., Vovelle F., Ptak M. Protein Sci. 5:565-577(1996) [PubMed: 8845747] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J04176 mRNA. Translation: AAA33493.1.
M57249 mRNA. Translation: AAA33494.1.

PIR

A31779.
S45635.

RefSeq

NP_001105311.1. NM_001111841.1.

UniGene

Zm.89638.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AFH	NMR	-	A	28-120	[»]
1FK0	X-ray	1.80	A	28-120	[»]
1FK1	X-ray	1.80	A	28-120	[»]
1FK2	X-ray	1.80	A	28-120	[»]
1FK3	X-ray	1.80	A	28-120	[»]
1FK4	X-ray	1.80	A	28-120	[»]
1FK5	X-ray	1.30	A	28-120	[»]
1FK6	X-ray	1.90	A	28-120	[»]
1FK7	X-ray	1.90	A	28-120	[»]
1MZL	X-ray	1.90	A	28-120	[»]
1MZM	X-ray	1.78	A	28-120	[»]

ProteinModelPortal

P19656.

SMR

P19656. Positions 28-120.

ModBase

Search...

Protein family/group databases

Allergome

3534. Zea m 14.0101.
3535. Zea m 14.0102.
684. Zea m 14.

Proteomic databases

PRIDE

P19656.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

542231.

KEGG

zma:542231.

Organism-specific databases

Gramene

P19656.

MaizeGDB

25447.

Phylogenomic databases

GeneTree

EPGT00050000004582.

Family and domain databases

InterPro

IPR016140. Bifunc_inhib/LTP/seed_store.
IPR013770. Lipid_transfer_prot_hlx-dom.
IPR003612. LTP/seed_store/tryp_amyl_inhib.
IPR000528. Plant_LTP.
[Graphical view]

Gene3D

G3DSA:1.10.110.10. LPT_helical. 1 hit.

Pfam

PF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]

PRINTS

PR00382. LIPIDTRNSFER.

SMART

SM00499. AAI. 1 hit.
[Graphical view]

SUPFAM

SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.

PROSITE

PS00597. PLANT_LTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NLTP_MAIZE

Accession

Primary (citable) accession number: P19656

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program