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P19643

- AOFB_RAT

UniProt

P19643 - AOFB_RAT

Protein

Amine oxidase [flavin-containing] B

Gene

Maob

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.

    Catalytic activityi

    RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.

    Cofactori

    FAD.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei156 – 1561Important for catalytic activityBy similarity
    Sitei365 – 3651Important for catalytic activityBy similarity
    Sitei382 – 3821Important for catalytic activityBy similarity

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: RGD
    2. primary amine oxidase activity Source: RGD
    3. protein homodimerization activity Source: RGD

    GO - Biological processi

    1. negative regulation of serotonin secretion Source: RGD
    2. neurotransmitter catabolic process Source: RGD
    3. positive regulation of dopamine metabolic process Source: RGD
    4. response to aluminum ion Source: RGD
    5. response to corticosterone Source: RGD
    6. response to drug Source: RGD
    7. response to ethanol Source: RGD
    8. response to lipopolysaccharide Source: RGD
    9. response to selenium ion Source: RGD
    10. response to steroid hormone Source: RGD
    11. response to toxic substance Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BRENDAi1.4.3.4. 5301.
    SABIO-RKP19643.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amine oxidase [flavin-containing] B (EC:1.4.3.4)
    Alternative name(s):
    Monoamine oxidase type B
    Short name:
    MAO-B
    Gene namesi
    Name:Maob
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3041. Maob.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: Ensembl
    3. mitochondrial outer membrane Source: RGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi139 – 1391L → H: No change in substrate affinity.
    Mutagenesisi199 – 1991I → F: Increased affinity for serotonin and tyramine. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 520519Amine oxidase [flavin-containing] BPRO_0000099863Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei52 – 521N6-acetyllysineBy similarity
    Modified residuei397 – 3971S-8alpha-FAD cysteineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP19643.
    PRIDEiP19643.

    Expressioni

    Gene expression databases

    GenevestigatoriP19643.

    Interactioni

    Subunit structurei

    Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP19643.
    SMRiP19643. Positions 3-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 489488CytoplasmicBy similarityAdd
    BLAST
    Topological domaini517 – 5204Mitochondrial intermembraneBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei490 – 51627Helical; Anchor for type IV membrane proteinBy similarityAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi36 – 5217Arg/Lys-rich (basic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the flavin monoamine oxidase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1231.
    HOGENOMiHOG000221615.
    HOVERGENiHBG004255.
    InParanoidiP19643.
    KOiK00274.
    PhylomeDBiP19643.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00757. AMINEOXDASEF.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19643-1 [UniParc]FASTAAdd to Basket

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    MSNKCDVIVV GGGISGMAAA KLLHDCGLSV VVLEARDRVG GRTYTIRNKN    50
    VKYVDLGGSY VGPTQNRILR LAKELGLETY KVNEVERLIH FVKGKSYAFR 100
    GPFPPVWNPI TYLDYNNLWR TMDEMGQEIP SDAPWKAPLA EEWDYMTMKE 150
    LLDKICWTNS TKQIATLFVN LCVTAETHEV SALWFLWYVK QCGGTTRIIS 200
    TTNGGQERKF IGGSGQVSER IKDILGDRVK LERPVIHIDQ TGENVVVKTL 250
    NHEIYEAKYV ISAIPPVLGM KIHHSPPLPI LRNQLITRVP LGSVIKCMVY 300
    YKEPFWRKKD FCGTMVIEGE EAPIAYTLDD TKPDGSCAAI MGFILAHKAR 350
    KLVRLTKEER LRKLCELYAK VLNSQEALQP VHYEEKNWCE EQYSGGCYTA 400
    YFPPGILTQY GRVLRQPVGK IFFAGTETAS HWSGYMEGAV EAGERAAREI 450
    LHAIGKIPED EIWQPEPESV DVPARPITNT FLERHLPSVP GLLKLLGLTT 500
    ILSATALGFL AHKKGLFVRF 520
    Length:520
    Mass (Da):58,459
    Last modified:January 23, 2007 - v3
    Checksum:iF2C230BC6CDE5840
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti38 – 381R → C in AAA41566. (PubMed:2974701)Curated
    Sequence conflicti335 – 3362GS → AG in AAA41566. (PubMed:2974701)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23601 mRNA. Translation: AAA41566.1.
    BC089814 mRNA. Translation: AAH89814.1.
    PIRiA31870.
    RefSeqiNP_037330.1. NM_013198.1.
    UniGeneiRn.6656.

    Genome annotation databases

    GeneIDi25750.
    KEGGirno:25750.
    UCSCiRGD:3041. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23601 mRNA. Translation: AAA41566.1 .
    BC089814 mRNA. Translation: AAH89814.1 .
    PIRi A31870.
    RefSeqi NP_037330.1. NM_013198.1.
    UniGenei Rn.6656.

    3D structure databases

    ProteinModelPortali P19643.
    SMRi P19643. Positions 3-501.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P19643.
    ChEMBLi CHEMBL2993.
    GuidetoPHARMACOLOGYi 2490.

    Proteomic databases

    PaxDbi P19643.
    PRIDEi P19643.

    Protocols and materials databases

    DNASUi 25750.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 25750.
    KEGGi rno:25750.
    UCSCi RGD:3041. rat.

    Organism-specific databases

    CTDi 4129.
    RGDi 3041. Maob.

    Phylogenomic databases

    eggNOGi COG1231.
    HOGENOMi HOG000221615.
    HOVERGENi HBG004255.
    InParanoidi P19643.
    KOi K00274.
    PhylomeDBi P19643.

    Enzyme and pathway databases

    BRENDAi 1.4.3.4. 5301.
    SABIO-RK P19643.

    Miscellaneous databases

    NextBioi 607939.
    PROi P19643.

    Gene expression databases

    Genevestigatori P19643.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00757. AMINEOXDASEF.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a cDNA for rat liver monoamine oxidase B."
      Ito A., Kuwahara T., Inadome S., Sagara Y.
      Biochem. Biophys. Res. Commun. 157:970-976(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    3. "A key amino acid responsible for substrate selectivity of monoamine oxidase A and B."
      Tsugeno Y., Ito A.
      J. Biol. Chem. 272:14033-14036(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ILE-199.

    Entry informationi

    Entry nameiAOFB_RAT
    AccessioniPrimary (citable) accession number: P19643
    Secondary accession number(s): Q5EBB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 115 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3