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Reviewed, UniProtKB/Swiss-Prot P19643 (AOFB_RAT)

Last modified June 16, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Amine oxidase [flavin-containing] B
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase type B
      Short name=MAO-B
Gene names
Name: Maob
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.

Catalytic activity

RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.

Cofactor

FAD.

Subunit structure

Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.

Subcellular location

Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.

Sequence similarities

Belongs to the flavin monoamine oxidase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 520519Amine oxidase [flavin-containing] B
PRO_0000099863

Regions

Topological domain2 – 489488Cytoplasmic By similarity
Transmembrane490 – 51627Anchor for type IV membrane protein By similarity
Topological domain517 – 5204Mitochondrial intermembrane By similarity
Compositional bias36 – 5217Arg/Lys-rich (basic)

Sites

Site1561Important for catalytic activity By similarity
Site3651Important for catalytic activity By similarity
Site3821Important for catalytic activity By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue521N6-acetyllysine By similarity
Modified residue3971S-8alpha-FAD cysteine By similarity

Experimental info

Mutagenesis1391L → H: No change in substrate affinity.
Mutagenesis1991I → F: Increased affinity for serotonin and tyramine. Ref.3
Sequence conflict381R → C in AAA41566. Ref.1
Sequence conflict335 – 3362GS → AG in AAA41566. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P19643-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: F2C230BC6CDE5840

FASTA52058,459
        10         20         30         40         50         60 
MSNKCDVIVV GGGISGMAAA KLLHDCGLSV VVLEARDRVG GRTYTIRNKN VKYVDLGGSY 

        70         80         90        100        110        120 
VGPTQNRILR LAKELGLETY KVNEVERLIH FVKGKSYAFR GPFPPVWNPI TYLDYNNLWR 

       130        140        150        160        170        180 
TMDEMGQEIP SDAPWKAPLA EEWDYMTMKE LLDKICWTNS TKQIATLFVN LCVTAETHEV 

       190        200        210        220        230        240 
SALWFLWYVK QCGGTTRIIS TTNGGQERKF IGGSGQVSER IKDILGDRVK LERPVIHIDQ 

       250        260        270        280        290        300 
TGENVVVKTL NHEIYEAKYV ISAIPPVLGM KIHHSPPLPI LRNQLITRVP LGSVIKCMVY 

       310        320        330        340        350        360 
YKEPFWRKKD FCGTMVIEGE EAPIAYTLDD TKPDGSCAAI MGFILAHKAR KLVRLTKEER 

       370        380        390        400        410        420 
LRKLCELYAK VLNSQEALQP VHYEEKNWCE EQYSGGCYTA YFPPGILTQY GRVLRQPVGK 

       430        440        450        460        470        480 
IFFAGTETAS HWSGYMEGAV EAGERAAREI LHAIGKIPED EIWQPEPESV DVPARPITNT 

       490        500        510        520 
FLERHLPSVP GLLKLLGLTT ILSATALGFL AHKKGLFVRF

« Hide

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References

« Hide 'large scale' references
[1]"Molecular cloning of a cDNA for rat liver monoamine oxidase B."
Ito A., Kuwahara T., Inadome S., Sagara Y.
Biochem. Biophys. Res. Commun. 157:970-976(1988) [PubMed: 2974701] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[3]"A key amino acid responsible for substrate selectivity of monoamine oxidase A and B."
Tsugeno Y., Ito A.
J. Biol. Chem. 272:14033-14036(1997) [PubMed: 9162023] [Abstract]
Cited for: MUTAGENESIS OF ILE-199.

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Cross-references

Sequence databases

M23601 mRNA. Translation: AAA41566.1.
BC089814 mRNA. Translation: AAH89814.1.
IPIIPI00231774.
PIRA31870.
RefSeqNP_037330.1.
UniGeneRn.6656

3D structure databases

HSSPHSSP built from PDB template 1GOS based on UniProtKB P27338.
SMRP19643. Positions 3-501.
ModBaseSearch...

Genome annotation databases

EnsemblENSRNOG00000029778. Rattus norvegicus. [Contig view]
GeneID25750.
KEGGrno:25750.

Organism-specific databases

RGD3041. Maob.

Phylogenomic databases

HOVERGENP19643.

Enzyme and pathway databases

BRENDA1.4.3.4. 248.

Gene expression databases

ArrayExpressP19643.
GermOnlineENSRNOG00000029778. Rattus norvegicus.

Family and domain databases

InterProIPR001613. Amineoxid_fl.
IPR002937. Amino_oxidase.
[Graphical view]
PfamPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSPR00757. AMINEOXDASEF.
ProtoNetSearch...

Other Resources

NextBio607939.

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Entry information

Entry nameAOFB_RAT
AccessionPrimary (citable) accession number: P19643
Secondary accession number(s): Q5EBB5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 74 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents