Reviewed, UniProtKB/Swiss-Prot P19643 (AOFB_RAT)

Names and origin

Protein names

Recommended name:
    Amine oxidase [flavin-containing] B
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase type B
      Short name=MAO-B

Gene names

Name:

Maob

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
Catalytic activity	RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.
Cofactor	FAD.
Subunit structure	Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	neurotransmitter catabolic process Traceable author statement. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Traceable author statement. Source: RGD
Molecular function	amine oxidase activity Traceable author statement. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 520	519	Amine oxidase [flavin-containing] B		PRO_0000099863
Regions
	Topological domain	2 – 489	488	Cytoplasmic By similarity
	Transmembrane	490 – 516	27	Anchor for type IV membrane protein By similarity
	Topological domain	517 – 520	4	Mitochondrial intermembrane By similarity
	Compositional bias	36 – 52	17	Arg/Lys-rich (basic)
Sites
	Site	156	1	Important for catalytic activity By similarity
	Site	365	1	Important for catalytic activity By similarity
	Site	382	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	52	1	N6-acetyllysine By similarity
	Modified residue	397	1	S-8alpha-FAD cysteine By similarity
Experimental info
	Mutagenesis	139	1	L → H: No change in substrate affinity.
	Mutagenesis	199	1	I → F: Increased affinity for serotonin and tyramine. Ref.3
	Sequence conflict	38	1	R → C in AAA41566. Ref.1
	Sequence conflict	335 – 336	2	GS → AG in AAA41566. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P19643-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F2C230BC6CDE5840	FASTA	520	58,459
	10 20 30 40 50 60 MSNKCDVIVV GGGISGMAAA KLLHDCGLSV VVLEARDRVG GRTYTIRNKN VKYVDLGGSY 70 80 90 100 110 120 VGPTQNRILR LAKELGLETY KVNEVERLIH FVKGKSYAFR GPFPPVWNPI TYLDYNNLWR 130 140 150 160 170 180 TMDEMGQEIP SDAPWKAPLA EEWDYMTMKE LLDKICWTNS TKQIATLFVN LCVTAETHEV 190 200 210 220 230 240 SALWFLWYVK QCGGTTRIIS TTNGGQERKF IGGSGQVSER IKDILGDRVK LERPVIHIDQ 250 260 270 280 290 300 TGENVVVKTL NHEIYEAKYV ISAIPPVLGM KIHHSPPLPI LRNQLITRVP LGSVIKCMVY 310 320 330 340 350 360 YKEPFWRKKD FCGTMVIEGE EAPIAYTLDD TKPDGSCAAI MGFILAHKAR KLVRLTKEER 370 380 390 400 410 420 LRKLCELYAK VLNSQEALQP VHYEEKNWCE EQYSGGCYTA YFPPGILTQY GRVLRQPVGK 430 440 450 460 470 480 IFFAGTETAS HWSGYMEGAV EAGERAAREI LHAIGKIPED EIWQPEPESV DVPARPITNT 490 500 510 520 FLERHLPSVP GLLKLLGLTT ILSATALGFL AHKKGLFVRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a cDNA for rat liver monoamine oxidase B." Ito A., Kuwahara T., Inadome S., Sagara Y. Biochem. Biophys. Res. Commun. 157:970-976(1988) [PubMed: 2974701] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[3]	"A key amino acid responsible for substrate selectivity of monoamine oxidase A and B." Tsugeno Y., Ito A. J. Biol. Chem. 272:14033-14036(1997) [PubMed: 9162023] [Abstract] Cited for: MUTAGENESIS OF ILE-199.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M23601 mRNA. Translation: AAA41566.1. BC089814 mRNA. Translation: AAH89814.1.
IPI	IPI00231774.
PIR	A31870.
RefSeq	NP_037330.1.
UniGene	Rn.6656
3D structure databases
HSSP	HSSP built from PDB template 1GOS based on UniProtKB P27338.
SMR	P19643. Positions 3-501.
ModBase	Search...
Protein-protein interaction databases
STRING	P19643.
Genome annotation databases
Ensembl	ENSRNOT00000044009; ENSRNOP00000043466; ENSRNOG00000029778; Rattus norvegicus. [Genome view]
GeneID	25750.
KEGG	rno:25750.
UCSC	NM_013198. rat.
Organism-specific databases
CTD	25750.
RGD	3041. Maob.
Phylogenomic databases
HOVERGEN	P19643.
Enzyme and pathway databases
BRENDA	1.4.3.4. 248.
Gene expression databases
Genevestigator	P19643.
GermOnline	ENSRNOG00000029778. Rattus norvegicus.
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...
Other Resources
NextBio	607939.

Entry information

Entry name

AOFB_RAT

Accession

Primary (citable) accession number: P19643
Secondary accession number(s): Q5EBB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)