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Protein

PTS system maltose- and glucose-specific EIICB component

Gene

malX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in maltose and glucose transport.
MalX encodes a phosphotransferase system enzyme II that can recognize glucose and maltose as substrates even though these sugars may not represent the natural substrates of the system.

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + maltose(Side 1) = [protein]-L-histidine + maltose 6'-phosphate(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei471 – 4711Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • carbohydrate utilization Source: CACAO
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MALX-MONOMER.
ECOL316407:JW1613-MONOMER.

Protein family/group databases

TCDBi4.A.1.1.3. the pts glucose-glucoside (glc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system maltose- and glucose-specific EIICB component
Including the following 2 domains:
Maltose and glucose permease IIC component
Alternative name(s):
PTS system maltose- and glucose-specific EIIC component
Maltose- and glucose-specific phosphotransferase enzyme IIB component (EC:2.7.1.208)
Alternative name(s):
PTS system maltose- and glucose-specific EIIB component
Gene namesi
Name:malX
Ordered Locus Names:b1621, JW1613
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10563. malX.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei22 – 4221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei69 – 8921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei96 – 11621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei138 – 15821HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei189 – 20921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei289 – 30921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei321 – 34121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei343 – 36321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei369 – 38921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei399 – 41921HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530PTS system maltose- and glucose-specific EIICB componentPRO_0000186657Add
BLAST

Proteomic databases

PaxDbiP19642.
PRIDEiP19642.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
pykAP215993EBI-556578,EBI-368956

Protein-protein interaction databases

BioGridi4261128. 20 interactions.
DIPiDIP-10150N.
IntActiP19642. 1 interaction.
MINTiMINT-1239961.
STRINGi511145.b1621.

Structurei

3D structure databases

ProteinModelPortaliP19642.
SMRiP19642. Positions 453-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 431431PTS EIIC type-1PROSITE-ProRule annotationAdd
BLAST
Domaini449 – 53082PTS EIIB type-1PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similaritiesi

Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CI1. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
HOGENOMiHOG000250995.
InParanoidiP19642.
KOiK02790.
K02791.
OMAiGTMDVCG.
PhylomeDBiP19642.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR018113. PTrfase_EIIB_Cys.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011301. PTS_Mal/Glc-sp_IIBC_component.
IPR004719. PTS_maltose/Glc_sub_IIC.
[Graphical view]
PfamiPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR02004. PTS-IIBC-malX. 1 hit.
PROSITEiPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAKTAPKVT LWEFFQQLGK TFMLPVALLS FCGIMLGIGS SLSSHDVITL
60 70 80 90 100
IPVLGNPVLQ AIFTWMSKIG SFAFSFLPVM FCIAIPLGLA RENKGVAAFA
110 120 130 140 150
GFIGYAVMNL AVNFWLTNKG ILPTTDAAVL KANNIQSILG IQSIDTGILG
160 170 180 190 200
AVIAGIIVWM LHERFHNIRL PDALAFFGGT RFVPIISSLV MGLVGLVIPL
210 220 230 240 250
VWPIFAMGIS GLGHMINSAG DFGPMLFGTG ERLLLPFGLH HILVALIRFT
260 270 280 290 300
DAGGTQEVCG QTVSGALTIF QAQLSCPTTH GFSESATRFL SQGKMPAFLG
310 320 330 340 350
GLPGAALAMY HCARPENRHK IKGLLISGLI ACVVGGTTEP LEFLFLFVAP
360 370 380 390 400
VLYVIHALLT GLGFTVMSVL GVTIGNTDGN IIDFVVFGIL HGLSTKWYMV
410 420 430 440 450
PVVAAIWFVV YYVIFRFAIT RFNLKTPGRD SEVASSIEKA VAGAPGKSGY
460 470 480 490 500
NVPAILEALG GADNIVSLDN CITRLRLSVK DMSLVNVQAL KDNRAIGVVQ
510 520 530
LNQHNLQVVI GPQVQSVKDE MAGLMHTVQA
Length:530
Mass (Da):56,627
Last modified:November 1, 1997 - v3
Checksum:i042E9817955975BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441I → Y in AAA24098 (PubMed:1856179).Curated
Sequence conflicti296 – 2961P → N in AAA24098 (PubMed:1856179).Curated
Sequence conflicti432 – 4321E → R in AAA24098 (PubMed:1856179).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60722 mRNA. Translation: AAA24098.1.
U00096 Genomic DNA. Translation: AAC74693.1.
AP009048 Genomic DNA. Translation: BAA15372.1.
M28539 Genomic DNA. Translation: AAA24103.2. Sequence problems.
PIRiG64918.
RefSeqiNP_416138.1. NC_000913.3.
WP_000125583.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74693; AAC74693; b1621.
BAA15372; BAA15372; BAA15372.
GeneIDi946009.
KEGGiecj:JW1613.
eco:b1621.
PATRICi32118544. VBIEscCol129921_1692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60722 mRNA. Translation: AAA24098.1.
U00096 Genomic DNA. Translation: AAC74693.1.
AP009048 Genomic DNA. Translation: BAA15372.1.
M28539 Genomic DNA. Translation: AAA24103.2. Sequence problems.
PIRiG64918.
RefSeqiNP_416138.1. NC_000913.3.
WP_000125583.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP19642.
SMRiP19642. Positions 453-521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261128. 20 interactions.
DIPiDIP-10150N.
IntActiP19642. 1 interaction.
MINTiMINT-1239961.
STRINGi511145.b1621.

Protein family/group databases

TCDBi4.A.1.1.3. the pts glucose-glucoside (glc) family.

Proteomic databases

PaxDbiP19642.
PRIDEiP19642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74693; AAC74693; b1621.
BAA15372; BAA15372; BAA15372.
GeneIDi946009.
KEGGiecj:JW1613.
eco:b1621.
PATRICi32118544. VBIEscCol129921_1692.

Organism-specific databases

EchoBASEiEB0558.
EcoGeneiEG10563. malX.

Phylogenomic databases

eggNOGiENOG4105CI1. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
HOGENOMiHOG000250995.
InParanoidiP19642.
KOiK02790.
K02791.
OMAiGTMDVCG.
PhylomeDBiP19642.

Enzyme and pathway databases

BioCyciEcoCyc:MALX-MONOMER.
ECOL316407:JW1613-MONOMER.

Miscellaneous databases

PROiP19642.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR018113. PTrfase_EIIB_Cys.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011301. PTS_Mal/Glc-sp_IIBC_component.
IPR004719. PTS_maltose/Glc_sub_IIC.
[Graphical view]
PfamiPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR02004. PTS-IIBC-malX. 1 hit.
PROSITEiPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTOCB_ECOLI
AccessioniPrimary (citable) accession number: P19642
Secondary accession number(s): P77621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.