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Protein

PTS system maltose-specific EIICB component

Gene

malX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in maltose transport. MalX can also recognize and transport glucose even though this sugar may not represent the natural substrate of the system.1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + maltose(Side 1) = [protein]-L-histidine + maltose 6'-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei471Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate utilization Source: CACAO
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MALX-MONOMER.
ECOL316407:JW1613-MONOMER.
MetaCyc:MALX-MONOMER.

Protein family/group databases

TCDBi4.A.1.1.3. the pts glucose-glucoside (glc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system maltose-specific EIICB component1 Publication
Including the following 2 domains:
Maltose permease IIC component1 Publication
Alternative name(s):
PTS system maltose-specific EIIC component1 Publication
Maltose-specific phosphotransferase enzyme IIB component1 Publication (EC:2.7.1.208By similarity)
Alternative name(s):
PTS system maltose-specific EIIB component1 Publication
Gene namesi
Name:malX1 Publication
Ordered Locus Names:b1621, JW1613
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10563. malX.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei22 – 42HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei69 – 89HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei96 – 116HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei138 – 158HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei189 – 209HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei289 – 309HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei321 – 341HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei343 – 363HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei369 – 389HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei399 – 419HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001866571 – 530PTS system maltose-specific EIICB componentAdd BLAST530

Proteomic databases

PaxDbiP19642.
PRIDEiP19642.

Expressioni

Inductioni

By maltose. Repressed by MalI.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
pykAP215993EBI-556578,EBI-368956

Protein-protein interaction databases

BioGridi4261128. 20 interactors.
DIPiDIP-10150N.
IntActiP19642. 1 interactor.
MINTiMINT-1239961.
STRINGi511145.b1621.

Structurei

3D structure databases

ProteinModelPortaliP19642.
SMRiP19642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 431PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST431
Domaini449 – 530PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST82

Domaini

The EIIC domain type-1 forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The PTS EIIB type-1 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-1 domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CI1. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
HOGENOMiHOG000250995.
InParanoidiP19642.
KOiK02790.
K02791.
OMAiGTMDVCG.
PhylomeDBiP19642.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR018113. PTrfase_EIIB_Cys.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011301. PTS_Mal/Glc-sp_IIBC_component.
IPR004719. PTS_maltose/Glc_sub_IIC.
[Graphical view]
PfamiPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR02004. PTS-IIBC-malX. 1 hit.
PROSITEiPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAKTAPKVT LWEFFQQLGK TFMLPVALLS FCGIMLGIGS SLSSHDVITL
60 70 80 90 100
IPVLGNPVLQ AIFTWMSKIG SFAFSFLPVM FCIAIPLGLA RENKGVAAFA
110 120 130 140 150
GFIGYAVMNL AVNFWLTNKG ILPTTDAAVL KANNIQSILG IQSIDTGILG
160 170 180 190 200
AVIAGIIVWM LHERFHNIRL PDALAFFGGT RFVPIISSLV MGLVGLVIPL
210 220 230 240 250
VWPIFAMGIS GLGHMINSAG DFGPMLFGTG ERLLLPFGLH HILVALIRFT
260 270 280 290 300
DAGGTQEVCG QTVSGALTIF QAQLSCPTTH GFSESATRFL SQGKMPAFLG
310 320 330 340 350
GLPGAALAMY HCARPENRHK IKGLLISGLI ACVVGGTTEP LEFLFLFVAP
360 370 380 390 400
VLYVIHALLT GLGFTVMSVL GVTIGNTDGN IIDFVVFGIL HGLSTKWYMV
410 420 430 440 450
PVVAAIWFVV YYVIFRFAIT RFNLKTPGRD SEVASSIEKA VAGAPGKSGY
460 470 480 490 500
NVPAILEALG GADNIVSLDN CITRLRLSVK DMSLVNVQAL KDNRAIGVVQ
510 520 530
LNQHNLQVVI GPQVQSVKDE MAGLMHTVQA
Length:530
Mass (Da):56,627
Last modified:November 1, 1997 - v3
Checksum:i042E9817955975BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144I → Y in AAA24098 (PubMed:1856179).Curated1
Sequence conflicti296P → N in AAA24098 (PubMed:1856179).Curated1
Sequence conflicti432E → R in AAA24098 (PubMed:1856179).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60722 mRNA. Translation: AAA24098.1.
U00096 Genomic DNA. Translation: AAC74693.1.
AP009048 Genomic DNA. Translation: BAA15372.1.
M28539 Genomic DNA. Translation: AAA24103.2. Sequence problems.
PIRiG64918.
RefSeqiNP_416138.1. NC_000913.3.
WP_000125583.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74693; AAC74693; b1621.
BAA15372; BAA15372; BAA15372.
GeneIDi946009.
KEGGiecj:JW1613.
eco:b1621.
PATRICi32118544. VBIEscCol129921_1692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60722 mRNA. Translation: AAA24098.1.
U00096 Genomic DNA. Translation: AAC74693.1.
AP009048 Genomic DNA. Translation: BAA15372.1.
M28539 Genomic DNA. Translation: AAA24103.2. Sequence problems.
PIRiG64918.
RefSeqiNP_416138.1. NC_000913.3.
WP_000125583.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP19642.
SMRiP19642.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261128. 20 interactors.
DIPiDIP-10150N.
IntActiP19642. 1 interactor.
MINTiMINT-1239961.
STRINGi511145.b1621.

Protein family/group databases

TCDBi4.A.1.1.3. the pts glucose-glucoside (glc) family.

Proteomic databases

PaxDbiP19642.
PRIDEiP19642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74693; AAC74693; b1621.
BAA15372; BAA15372; BAA15372.
GeneIDi946009.
KEGGiecj:JW1613.
eco:b1621.
PATRICi32118544. VBIEscCol129921_1692.

Organism-specific databases

EchoBASEiEB0558.
EcoGeneiEG10563. malX.

Phylogenomic databases

eggNOGiENOG4105CI1. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
HOGENOMiHOG000250995.
InParanoidiP19642.
KOiK02790.
K02791.
OMAiGTMDVCG.
PhylomeDBiP19642.

Enzyme and pathway databases

BioCyciEcoCyc:MALX-MONOMER.
ECOL316407:JW1613-MONOMER.
MetaCyc:MALX-MONOMER.

Miscellaneous databases

PROiP19642.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR018113. PTrfase_EIIB_Cys.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011301. PTS_Mal/Glc-sp_IIBC_component.
IPR004719. PTS_maltose/Glc_sub_IIC.
[Graphical view]
PfamiPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00852. pts-Glc. 1 hit.
TIGR02004. PTS-IIBC-malX. 1 hit.
PROSITEiPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTOCB_ECOLI
AccessioniPrimary (citable) accession number: P19642
Secondary accession number(s): P77621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.