PTS system maltose- and glucose-specific EIICB component

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P19642 (PTOCB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 130. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
PTS system maltose- and glucose-specific EIICB component

Including the following 2 domains:

Maltose and glucose permease IIC component
Alternative name(s):
PTS system maltose- and glucose-specific EIIC component
Maltose- and glucose-specific phosphotransferase enzyme IIB component
EC=2.7.1.69
Alternative name(s):
PTS system maltose- and glucose-specific EIIB component

Gene names

Name:	malX
Ordered Locus Names:	b1621, JW1613

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in maltose and glucose transport. MalX encodes a phosphotransferase system enzyme II that can recognize glucose and maltose as substrates even though these sugars may not represent the natural substrates of the system.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.6.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 530

530

PTS system maltose- and glucose-specific EIICB component

PRO_0000186657

Regions

Transmembrane

22 – 42

Helical; Potential

Transmembrane

69 – 89

Helical; Potential

Transmembrane

96 – 116

Helical; Potential

Transmembrane

138 – 158

Helical; Potential

Transmembrane

189 – 209

Helical; Potential

Transmembrane

289 – 309

Helical; Potential

Transmembrane

321 – 341

Helical; Potential

Transmembrane

343 – 363

Helical; Potential

Transmembrane

369 – 389

Helical; Potential

Transmembrane

399 – 419

Helical; Potential

Domain

1 – 431

431

PTS EIIC type-1

Domain

449 – 530

PTS EIIB type-1

Sites

Active site

471

Phosphocysteine intermediate; for EIIB activity By similarity

Experimental info

Sequence conflict

144

I → Y in AAA24098. Ref.1

Sequence conflict

296

P → N in AAA24098. Ref.1

Sequence conflict

432

E → R in AAA24098. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P19642 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 042E9817955975BF
Show »

FASTA

530

56,627

        10         20         30         40         50         60 
MTAKTAPKVT LWEFFQQLGK TFMLPVALLS FCGIMLGIGS SLSSHDVITL IPVLGNPVLQ 

        70         80         90        100        110        120 
AIFTWMSKIG SFAFSFLPVM FCIAIPLGLA RENKGVAAFA GFIGYAVMNL AVNFWLTNKG 

       130        140        150        160        170        180 
ILPTTDAAVL KANNIQSILG IQSIDTGILG AVIAGIIVWM LHERFHNIRL PDALAFFGGT 

       190        200        210        220        230        240 
RFVPIISSLV MGLVGLVIPL VWPIFAMGIS GLGHMINSAG DFGPMLFGTG ERLLLPFGLH 

       250        260        270        280        290        300 
HILVALIRFT DAGGTQEVCG QTVSGALTIF QAQLSCPTTH GFSESATRFL SQGKMPAFLG 

       310        320        330        340        350        360 
GLPGAALAMY HCARPENRHK IKGLLISGLI ACVVGGTTEP LEFLFLFVAP VLYVIHALLT 

       370        380        390        400        410        420 
GLGFTVMSVL GVTIGNTDGN IIDFVVFGIL HGLSTKWYMV PVVAAIWFVV YYVIFRFAIT 

       430        440        450        460        470        480 
RFNLKTPGRD SEVASSIEKA VAGAPGKSGY NVPAILEALG GADNIVSLDN CITRLRLSVK 

       490        500        510        520        530 
DMSLVNVQAL KDNRAIGVVQ LNQHNLQVVI GPQVQSVKDE MAGLMHTVQA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The malX malY operon of Escherichia coli encodes a novel enzyme II of the phosphotransferase system recognizing glucose and maltose and an enzyme abolishing the endogenous induction of the maltose system." Reidl J., Boos W. J. Bacteriol. 173:4862-4876(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"MalI, a novel protein involved in regulation of the maltose system of Escherichia coli, is highly homologous to the repressor proteins GalR, CytR, and LacI." Reidl J., Roemisch K., Ehrmann M., Boos W. J. Bacteriol. 171:4888-4899(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-177.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60722 mRNA. Translation: AAA24098.1. U00096 Genomic DNA. Translation: AAC74693.1. AP009048 Genomic DNA. Translation: BAA15372.1. M28539 Genomic DNA. Translation: AAA24103.2. Sequence problems.
PIR	G64918.
RefSeq	NP_416138.1. NC_000913.2. YP_489884.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P19642.
SMR	P19642. Positions 453-521.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10150N.
IntAct	P19642. 1 interaction.
MINT	MINT-1239961.
STRING	511145.b1621.
Protein family/group databases
TCDB	4.A.1.1.3. PTS glucose-glucoside (Glc) family.
Proteomic databases
PRIDE	P19642.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74693; AAC74693; b1621. BAA15372; BAA15372; BAA15372.
GeneID	12932134. 946009.
KEGG	ecj:Y75_p1597. eco:b1621.
PATRIC	32118544. VBIEscCol129921_1692.
Organism-specific databases
EchoBASE	EB0558.
EcoGene	EG10563. malX.
Phylogenomic databases
eggNOG	COG1263.
HOGENOM	HOG000250995.
KO	K02790. K02791.
OMA	MSKVGSF.
ProtClustDB	PRK10110.
Enzyme and pathway databases
BioCyc	EcoCyc:MALX-MONOMER. ECOL316407:JW1613-MONOMER.
Gene expression databases
Genevestigator	P19642.
Family and domain databases
Gene3D	3.30.1360.60. 1 hit.
InterPro	IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR004719. PTrfase_sys_maltose/Glc-sp_IIC. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011301. PTS_Mal/Glc-sp_IIBC_component. [Graphical view]
Pfam	PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
SUPFAM	SSF55604. PTS_EIIB. 1 hit.
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00852. pts-Glc. 1 hit. TIGR02004. PTS-IIBC-malX. 1 hit.
PROSITE	PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTOCB_ECOLI

Accession

Primary (citable) accession number: P19642
Secondary accession number(s): P77621

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 130 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents