Reviewed, UniProtKB/Swiss-Prot P19638 (UDPK_BACSU)

Names and origin

Protein names

Recommended name:
    Undecaprenol kinase
      Short name=UdpK
    EC=2.7.1.66

Gene names

Name:	dgkA
Synonyms:	dgk, yqxF
Ordered Locus Names:	BSU25310

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	123 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the phosphorylation of undecaprenol in vitro, which is probably the physiological substrate. Exhibits no detectable activity against other substrates such as monoacylglycerol, ceramide, or diacylglycerol (DAG). Appears indispensable for the maintenance of spore stability and viability in B.subtilis. Ref.8
Catalytic activity	ATP + undecaprenol = ADP + undecaprenyl phosphate. Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Induction	Mainly expressed during the vegetative phase of growth. Ref.7
Disruption phenotype	Cells lacking this gene exhibit an abnormal cortex structure in mutant endospores 6 hours after the onset of sporulation, an indication of cortex degeneration. In addition, they display a significant decrease in the dipicolinic acid content of mutant spores. Ref.7
Sequence similarities	Belongs to the bacterial diacylglycerol kinase family.
Caution	Was originally (Ref.7) thought to be a diacylglycerol kinase.
Sequence caution	The sequence BAA12480.1 differs from that shown. Reason: Frameshift at position 10.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis Sporulation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 123	123	Undecaprenol kinase		PRO_0000195259
Regions
	Topological domain	1 – 33	33	Cytoplasmic Potential
	Transmembrane	34 – 51	18	Potential
	Topological domain	52 – 57	6	Extracellular Potential
	Transmembrane	58 – 74	17	Potential
	Topological domain	75 – 99	25	Cytoplasmic Potential
	Transmembrane	100 – 120	21	Potential
	Topological domain	121 – 123	3	Extracellular Potential
Experimental info
	Sequence conflict	112	1	C → S in AAA70044. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P19638-1 [UniParc]. Last modified April 14, 2009. Version 4. Checksum: 7BED3954910AEBF9	FASTA	123	13,639
	10 20 30 40 50 60 MDSKDHRNEL NRFFKSFVHA GRGIWETART ERNFQFHAAA ACAVLICGFL VELSIIEWMI 70 80 90 100 110 120 IFLLIGGMFS LELLNTAIEH TVDLITDKHH PLAKAAKDAA AGAVCVFAVI SCIIGLLIFL PKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence upstream of the cdd locus in Bacillus subtilis." Kim K., Hwang S., Suh J., Song B.-H., Hong S., Kim J. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ED40.
[2]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO N-TERMINUS.
[5]	"Chromosomal location, cloning and nucleotide sequence of the Bacillus subtilis cdd gene encoding cytidine/deoxycytidine deaminase." Song B.-H., Neuhard J. Mol. Gen. Genet. 216:462-468(1989) [PubMed: 2526291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-123. Strain: 168.
[6]	"Membrane topology of Escherichia coli diacylglycerol kinase." Smith R.L., O'Toole J.F., Maguire M.E., Sanders C.R. II J. Bacteriol. 176:5459-5465(1994) [PubMed: 8071224] [Abstract] Cited for: IDENTIFICATION.
[7]	"Bacillus subtilis diacylglycerol kinase (DgkA) enhances efficient sporulation." Amiteye S., Kobayashi K., Imamura D., Hosoya S., Ogasawara N., Sato T. J. Bacteriol. 185:5306-5309(2003) [PubMed: 12923107] [Abstract] Cited for: INDUCTION, DISRUPTION PHENOTYPE.
[8]	"Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis." Jerga A., Lu Y.-J., Schujman G.E., de Mendoza D., Rock C.O. J. Biol. Chem. 282:21738-21745(2007) [PubMed: 17535816] [Abstract] Cited for: FUNCTION AS AN UNDECAPRENOL KINASE, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U29177 Genomic DNA. Translation: AAA70044.1. Different initiation. D84432 Genomic DNA. Translation: BAA12480.1. Frameshift. AL009126 Genomic DNA. Translation: CAB14460.2. X17430 Genomic DNA. Translation: CAB57857.1.
PIR	G69614.
RefSeq	NP_390409.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	937873.
GenomeReviews	Gene locus BSU25310 in contig AL009126_GR.
KEGG	bsu:BSU25310.
NMPDR	fig|224308.1.peg.2534.
Organism-specific databases
SubtiList	BG11040. dgkA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P19638.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2527-MON.
BRENDA	2.7.1.107. 150.
Family and domain databases
InterPro	IPR000829. Diacylglycerol_kinase_prok. [Graphical view]
Pfam	PF01219. DAGK_prokar. 1 hit. [Graphical view]
ProDom	PD010722. DAGK_prokar. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01069. DAGK_PROKAR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UDPK_BACSU

Accession

Primary (citable) accession number: P19638

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	April 14, 2009
Last modified:	October 13, 2009
This is version 81 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)