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Protein

Calsequestrin-1

Gene

Casq1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed:8042990). Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca2+ ions. Regulates the release of lumenal Ca2+ via the calcium release channel RYR1; this plays an important role in triggering muscle contraction (By similarity).By similarity1 Publication

GO - Molecular functioni

  • calcium ion binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-2672351. Stimuli-sensing channels.
R-RNO-5578775. Ion homeostasis.

Names & Taxonomyi

Protein namesi
Recommended name:
Calsequestrin-1
Alternative name(s):
Aspartactin
Calsequestrin, skeletal muscle isoform
Laminin-binding protein
Gene namesi
Name:Casq1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1586677. Casq1.

Subcellular locationi

  • Sarcoplasmic reticulum lumen By similarity
  • Sarcoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side By similarity
  • Mitochondrion matrix By similarity

  • Note: This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells.By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • Golgi apparatus Source: RGD
  • I band Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
  • mitochondrial matrix Source: UniProtKB
  • myofibril Source: RGD
  • sarcolemma Source: RGD
  • sarcoplasmic reticulum Source: RGD
  • sarcoplasmic reticulum lumen Source: UniProtKB
  • sarcoplasmic reticulum membrane Source: UniProtKB-SubCell
  • terminal cisterna Source: RGD
  • terminal cisterna lumen Source: RGD
  • T-tubule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Sequence analysis2 PublicationsAdd
BLAST
Chaini35 – 406372Calsequestrin-1PRO_0000144072Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431PhosphotyrosineCombined sources
Modified residuei81 – 811PhosphoserineCombined sources
Modified residuei124 – 1241PhosphothreonineCombined sources
Modified residuei216 – 2161PhosphoserineCombined sources
Modified residuei297 – 2971PhosphotyrosineBy similarity
Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP19633.

PTM databases

iPTMnetiP19633.
PhosphoSiteiP19633.

Expressioni

Tissue specificityi

Detected in skeletal muscle and in smooth muscle from vas deferens, aorta and stomach (at protein level).2 Publications

Gene expression databases

BgeeiENSRNOG00000006930.
GenevisibleiP19633. RN.

Interactioni

Subunit structurei

Monomer, homodimer, homotetramer and homopolymer. Can form linear homooligomers. Ca2+ ions promote oligomerization. Interacts with ASPH and TRDN.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009344.

Structurei

3D structure databases

ProteinModelPortaliP19633.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi353 – 40654Asp-richAdd
BLAST

Sequence similaritiesi

Belongs to the calsequestrin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGY5. Eukaryota.
ENOG4111R2M. LUCA.
GeneTreeiENSGT00390000019377.
InParanoidiP19633.
OMAiVYVFKGD.
OrthoDBiEOG091G08VX.

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR001393. Calsequestrin.
IPR018233. Calsequestrin_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF01216. Calsequestrin. 1 hit.
[Graphical view]
PRINTSiPR00312. CALSEQUESTRN.
SUPFAMiSSF52833. SSF52833. 3 hits.
PROSITEiPS00863. CALSEQUESTRIN_1. 1 hit.
PS00864. CALSEQUESTRIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19633-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRATDRMGAR AVSKLRLALL FVLVLGTPRS GVQGEDGLDF PEYDGVDRVI
60 70 80 90 100
NVNAKNYKNV FKKYEVLALL YHEPPEDDKA SQRQFEMEEL ILELAAQVLE
110 120 130 140 150
DKGVGFGLVD SEKDAAVAKK LGLTEEDSVY VFKGDEVIEY DGEFSADTLV
160 170 180 190 200
EFLLDVLEDP VELIEGEREL QAFENIEDEI KLIGYFKSKD SEHYKAYEDA
210 220 230 240 250
AEEFHPYIPF FATFDSKVAK KLTLKLNEID FYEAFMEEPV TIPDKPNSEE
260 270 280 290 300
EIVSFVEEHR RSTLRKLKPE SMYETWEDDL DGIHIVAFAE EADPDGYEFL
310 320 330 340 350
ETLKAVAQDN TENPDLSIIW IDPDDFPLLV PYWEKTFDID LSAPQIGVVN
360 370 380 390 400
VTDADSIWME MDDEEDLPSA EELEDWLEDV LEGEINTEDD DDDDDDDDDD

DDDDDD
Length:406
Mass (Da):46,449
Last modified:July 27, 2011 - v2
Checksum:iED408245EDA12E23
GO

Sequence cautioni

The sequence CA334109 differs from that shown. Reason: Frameshift at position 147. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361D → E AA sequence (PubMed:8042990).Curated
Sequence conflicti53 – 542NA → AD AA sequence (PubMed:8042990).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03085534 Genomic DNA. No translation available.
AABR03087242 Genomic DNA. No translation available.
CA334109 mRNA. No translation available.
PIRiA31049.
RefSeqiNP_001153066.1. NM_001159594.1.
UniGeneiRn.159963.

Genome annotation databases

EnsembliENSRNOT00000009344; ENSRNOP00000009344; ENSRNOG00000006930.
GeneIDi686019.
KEGGirno:686019.
UCSCiRGD:1586677. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03085534 Genomic DNA. No translation available.
AABR03087242 Genomic DNA. No translation available.
CA334109 mRNA. No translation available.
PIRiA31049.
RefSeqiNP_001153066.1. NM_001159594.1.
UniGeneiRn.159963.

3D structure databases

ProteinModelPortaliP19633.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009344.

PTM databases

iPTMnetiP19633.
PhosphoSiteiP19633.

Proteomic databases

PaxDbiP19633.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009344; ENSRNOP00000009344; ENSRNOG00000006930.
GeneIDi686019.
KEGGirno:686019.
UCSCiRGD:1586677. rat.

Organism-specific databases

CTDi844.
RGDi1586677. Casq1.

Phylogenomic databases

eggNOGiENOG410IGY5. Eukaryota.
ENOG4111R2M. LUCA.
GeneTreeiENSGT00390000019377.
InParanoidiP19633.
OMAiVYVFKGD.
OrthoDBiEOG091G08VX.

Enzyme and pathway databases

ReactomeiR-RNO-2672351. Stimuli-sensing channels.
R-RNO-5578775. Ion homeostasis.

Miscellaneous databases

PROiP19633.

Gene expression databases

BgeeiENSRNOG00000006930.
GenevisibleiP19633. RN.

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR001393. Calsequestrin.
IPR018233. Calsequestrin_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF01216. Calsequestrin. 1 hit.
[Graphical view]
PRINTSiPR00312. CALSEQUESTRN.
SUPFAMiSSF52833. SSF52833. 3 hits.
PROSITEiPS00863. CALSEQUESTRIN_1. 1 hit.
PS00864. CALSEQUESTRIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCASQ1_RAT
AccessioniPrimary (citable) accession number: P19633
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.