Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calsequestrin-1

Gene

Casq1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed:8042990). Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca2+ ions. Regulates the release of lumenal Ca2+ via the calcium release channel RYR1; this plays an important role in triggering muscle contraction (By similarity).By similarity1 Publication

GO - Molecular functioni

  • calcium ion binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionMuscle protein
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-2672351. Stimuli-sensing channels.
R-RNO-5578775. Ion homeostasis.

Names & Taxonomyi

Protein namesi
Recommended name:
Calsequestrin-1
Alternative name(s):
Aspartactin
Calsequestrin, skeletal muscle isoform
Laminin-binding protein
Gene namesi
Name:Casq1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1586677. Casq1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Sequence analysis2 PublicationsAdd BLAST34
ChainiPRO_000014407235 – 406Calsequestrin-1Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43PhosphotyrosineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei124PhosphothreonineCombined sources1
Modified residuei216PhosphoserineCombined sources1
Modified residuei297PhosphotyrosineBy similarity1
Glycosylationi350N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP19633.

PTM databases

iPTMnetiP19633.
PhosphoSitePlusiP19633.

Expressioni

Tissue specificityi

Detected in skeletal muscle and in smooth muscle from vas deferens, aorta and stomach (at protein level).2 Publications

Gene expression databases

BgeeiENSRNOG00000006930.
GenevisibleiP19633. RN.

Interactioni

Subunit structurei

Monomer, homodimer, homotetramer and homopolymer. Can form linear homooligomers. Ca2+ ions promote oligomerization. Interacts with ASPH and TRDN.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009344.

Structurei

3D structure databases

ProteinModelPortaliP19633.
SMRiP19633.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi353 – 406Asp-richAdd BLAST54

Sequence similaritiesi

Belongs to the calsequestrin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGY5. Eukaryota.
ENOG4111R2M. LUCA.
GeneTreeiENSGT00390000019377.
InParanoidiP19633.
OMAiSGVQGEE.
OrthoDBiEOG091G08VX.

Family and domain databases

InterProiView protein in InterPro
IPR001393. Calsequestrin.
IPR018233. Calsequestrin_CS.
IPR012336. Thioredoxin-like_fold.
PANTHERiPTHR10033. PTHR10033. 1 hit.
PfamiView protein in Pfam
PF01216. Calsequestrin. 1 hit.
SUPFAMiSSF52833. SSF52833. 3 hits.
PROSITEiView protein in PROSITE
PS00863. CALSEQUESTRIN_1. 1 hit.
PS00864. CALSEQUESTRIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19633-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRATDRMGAR AVSKLRLALL FVLVLGTPRS GVQGEDGLDF PEYDGVDRVI
60 70 80 90 100
NVNAKNYKNV FKKYEVLALL YHEPPEDDKA SQRQFEMEEL ILELAAQVLE
110 120 130 140 150
DKGVGFGLVD SEKDAAVAKK LGLTEEDSVY VFKGDEVIEY DGEFSADTLV
160 170 180 190 200
EFLLDVLEDP VELIEGEREL QAFENIEDEI KLIGYFKSKD SEHYKAYEDA
210 220 230 240 250
AEEFHPYIPF FATFDSKVAK KLTLKLNEID FYEAFMEEPV TIPDKPNSEE
260 270 280 290 300
EIVSFVEEHR RSTLRKLKPE SMYETWEDDL DGIHIVAFAE EADPDGYEFL
310 320 330 340 350
ETLKAVAQDN TENPDLSIIW IDPDDFPLLV PYWEKTFDID LSAPQIGVVN
360 370 380 390 400
VTDADSIWME MDDEEDLPSA EELEDWLEDV LEGEINTEDD DDDDDDDDDD

DDDDDD
Length:406
Mass (Da):46,449
Last modified:July 27, 2011 - v2
Checksum:iED408245EDA12E23
GO

Sequence cautioni

The sequence CA334109 differs from that shown. Reason: Frameshift at position 147.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36D → E AA sequence (PubMed:8042990).Curated1
Sequence conflicti53 – 54NA → AD AA sequence (PubMed:8042990).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03085534 Genomic DNA. No translation available.
AABR03087242 Genomic DNA. No translation available.
CA334109 mRNA. No translation available.
PIRiA31049.
RefSeqiNP_001153066.1. NM_001159594.1.
UniGeneiRn.159963.

Genome annotation databases

EnsembliENSRNOT00000009344; ENSRNOP00000009344; ENSRNOG00000006930.
GeneIDi686019.
KEGGirno:686019.
UCSCiRGD:1586677. rat.

Similar proteinsi

Entry informationi

Entry nameiCASQ1_RAT
AccessioniPrimary (citable) accession number: P19633
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 27, 2011
Last modified: September 27, 2017
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families