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Protein

Annexin A2

Gene

ANXA2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.

GO - Molecular functioni

  1. calcium-dependent phospholipid binding Source: UniProtKB-KW
  2. calcium ion binding Source: InterPro
  3. phospholipase inhibitor activity Source: InterPro
  4. S100 protein binding Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Annexin A2
Alternative name(s):
Annexin II
Annexin-2
Calpactin I heavy chain
Calpactin-1 heavy chain
Chromobindin-8
Lipocortin II
Placental anticoagulant protein IV
Short name:
PAP-IV
Protein I
p36
Gene namesi
Name:ANXA2
Synonyms:ANX2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Unplaced

Subcellular locationi

Secretedextracellular spaceextracellular matrixbasement membrane. Melanosome By similarity
Note: In the lamina beneath the plasma membrane.

GO - Cellular componenti

  1. basement membrane Source: UniProtKB-SubCell
  2. melanosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 339338Annexin A2PRO_0000067472Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei24 – 241Phosphotyrosine; by SRCBy similarity
Modified residuei26 – 261Phosphoserine; by PKCBy similarity
Modified residuei49 – 491N6-acetyllysineBy similarity
Modified residuei152 – 1521N6-acetyllysineBy similarity
Modified residuei199 – 1991PhosphotyrosineBy similarity
Modified residuei227 – 2271N6-acetyllysineBy similarity

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP19620.
PRIDEiP19620.

Interactioni

Subunit structurei

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF (By similarity). Interacts with COCH (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-1214816.
STRINGi9823.ENSSSCP00000004935.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati42 – 10261Annexin 1Add
BLAST
Repeati114 – 17461Annexin 2Add
BLAST
Repeati199 – 25961Annexin 3Add
BLAST
Repeati274 – 33461Annexin 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2423S100A10-binding siteAdd
BLAST

Domaini

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Sequence similaritiesi

Belongs to the annexin family.Curated
Contains 4 annexin repeats.Curated

Keywords - Domaini

Annexin, Repeat

Phylogenomic databases

eggNOGiNOG259189.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP19620.
KOiK17092.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERiPTHR10502:SF18. PTHR10502:SF18. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19620-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVHEILCK LSLEGDHSTP ASAYGSVKAY TNFDAERDAL NIETAIKTKG
60 70 80 90 100
VDEVTIVNIL TNRSNEQRQD IAFAYQRRTK KELASALKSA LSGHLETVIL
110 120 130 140 150
GLLKTPAQYD ASELKASMKG LGTDEDSLIE IICSRTNQEL QEINRVYKEM
160 170 180 190 200
YKTDLEKDII SDTSGDFRKL MVALAKGRRA EDGSVIDYEL IDQDARDLYD
210 220 230 240 250
AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM LESIKKEVKG
260 270 280 290 300
DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIX IMVSRSEVDM
310 320 330
LKIRSEFKRK YGKSLYNYIQ QDTKGDYQKA LLYLCGGDD
Length:339
Mass (Da):38,534
Last modified:May 29, 2007 - v4
Checksum:iFAC554639119900D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2231C → P AA sequence (PubMed:2937654).Curated
Sequence conflicti229 – 2291F → S AA sequence (PubMed:17607745).Curated
Sequence conflicti230 – 2301E → S AA sequence (PubMed:2937654).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY706383 mRNA. Translation: AAU85387.1.
PIRiS01128.
RefSeqiNP_001005726.1. NM_001005726.1.
UniGeneiSsc.12241.

Genome annotation databases

GeneIDi406192.
KEGGissc:406192.

Cross-referencesi

Web resourcesi

Protein Spotlight

Red velvet - Issue 86 of September 2007

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY706383 mRNA. Translation: AAU85387.1.
PIRiS01128.
RefSeqiNP_001005726.1. NM_001005726.1.
UniGeneiSsc.12241.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1214816.
STRINGi9823.ENSSSCP00000004935.

Proteomic databases

PaxDbiP19620.
PRIDEiP19620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi406192.
KEGGissc:406192.

Organism-specific databases

CTDi302.

Phylogenomic databases

eggNOGiNOG259189.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP19620.
KOiK17092.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERiPTHR10502:SF18. PTHR10502:SF18. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of metaphase II-specific gene transcripts in porcine oocytes and their expression in early stage embryos."
    Cui X.S., Song H., Kim N.H.
    Reprod. Fertil. Dev. 17:625-631(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Annexin A2 / p11 interaction: new insights into annexin A2 tetramer structure by chemical cross-linking, high-resolution mass spectrometry, and computational modeling."
    Schulz D.M., Kalkhof S., Schmidt A., Ihling C., Stingl C., Mechtler K., Zschoernig O., Sinz A.
    Proteins 69:254-269(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-339, ACETYLATION AT SER-2.
  3. "A discontinuous epitope on p36, the major substrate of src tyrosine-protein-kinase, brings the phosphorylation site into the neighbourhood of a consensus sequence for Ca2+/lipid-binding proteins."
    Johnsson N., Johnsson K., Weber K.
    FEBS Lett. 236:201-204(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-70.
  4. "p36, the major cytoplasmic substrate of src tyrosine protein kinase, binds to its p11 regulatory subunit via a short amino-terminal amphiphatic helix."
    Johnsson N., Marriott G., Weber K.
    EMBO J. 7:2435-2442(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-30.
  5. "Binding sites for calcium, lipid and p11 on p36, the substrate of retroviral tyrosine-specific protein kinases."
    Johnsson N., Vandekerckhove J., Van Damme J., Weber K.
    FEBS Lett. 198:361-364(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 213-234.

Entry informationi

Entry nameiANXA2_PIG
AccessioniPrimary (citable) accession number: P19620
Secondary accession number(s): Q5Y2C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 29, 2007
Last modified: March 4, 2015
This is version 125 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.