P19620 (ANXA2_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Annexin A2

Alternative name(s):
Annexin II
Annexin-2
Calpactin I heavy chain
Calpactin-1 heavy chain
Chromobindin-8
Lipocortin II
Placental anticoagulant protein IV
Short name=PAP-IV
Protein I
p36

Gene names

Name:	ANXA2
Synonyms:	ANX2

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.
Subunit structure	Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF By similarity. Interacts with COCH By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix › basement membrane. Melanosome By similarity. Note: In the lamina beneath the plasma membrane.
Domain	A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational modification	ISGylated By similarity.
Miscellaneous	It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Sequence similarities	Belongs to the annexin family. Contains 4 annexin repeats.

Ontologies

Keywords
Cellular component	Basement membrane Extracellular matrix Secreted
Domain	Annexin Repeat
Ligand	Calcium Calcium/phospholipid-binding
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	basement membrane Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent phospholipid binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2 Ref.3 Ref.4
	Chain	2 – 339	338	Annexin A2		PRO_0000067472
Regions
	Repeat	42 – 102	61	Annexin 1
	Repeat	114 – 174	61	Annexin 2
	Repeat	199 – 259	61	Annexin 3
	Repeat	274 – 334	61	Annexin 4
	Region	2 – 24	23	S100A10-binding site
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.2
	Modified residue	24	1	Phosphotyrosine; by SRC By similarity
	Modified residue	26	1	Phosphoserine; by PKC By similarity
Experimental info
	Sequence conflict	223	1	C → P AA sequence Ref.5
	Sequence conflict	229	1	F → S AA sequence Ref.2
	Sequence conflict	230	1	E → S AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P19620 [UniParc]. Last modified May 29, 2007. Version 4. Checksum: FAC554639119900D	FASTA	339	38,534
	10 20 30 40 50 60 MSTVHEILCK LSLEGDHSTP ASAYGSVKAY TNFDAERDAL NIETAIKTKG VDEVTIVNIL 70 80 90 100 110 120 TNRSNEQRQD IAFAYQRRTK KELASALKSA LSGHLETVIL GLLKTPAQYD ASELKASMKG 130 140 150 160 170 180 LGTDEDSLIE IICSRTNQEL QEINRVYKEM YKTDLEKDII SDTSGDFRKL MVALAKGRRA 190 200 210 220 230 240 EDGSVIDYEL IDQDARDLYD AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM 250 260 270 280 290 300 LESIKKEVKG DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIX IMVSRSEVDM 310 320 330 LKIRSEFKRK YGKSLYNYIQ QDTKGDYQKA LLYLCGGDD

References

[1]	"Identification of metaphase II-specific gene transcripts in porcine oocytes and their expression in early stage embryos." Cui X.S., Song H., Kim N.H. Reprod. Fertil. Dev. 17:625-631(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Annexin A2 / p11 interaction: new insights into annexin A2 tetramer structure by chemical cross-linking, high-resolution mass spectrometry, and computational modeling." Schulz D.M., Kalkhof S., Schmidt A., Ihling C., Stingl C., Mechtler K., Zschoernig O., Sinz A. Proteins 69:254-269(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-339, ACETYLATION AT SER-2.
[3]	"A discontinuous epitope on p36, the major substrate of src tyrosine-protein-kinase, brings the phosphorylation site into the neighbourhood of a consensus sequence for Ca2+/lipid-binding proteins." Johnsson N., Johnsson K., Weber K. FEBS Lett. 236:201-204(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-70.
[4]	"p36, the major cytoplasmic substrate of src tyrosine protein kinase, binds to its p11 regulatory subunit via a short amino-terminal amphiphatic helix." Johnsson N., Marriott G., Weber K. EMBO J. 7:2435-2442(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-30.
[5]	"Binding sites for calcium, lipid and p11 on p36, the substrate of retroviral tyrosine-specific protein kinases." Johnsson N., Vandekerckhove J., Van Damme J., Weber K. FEBS Lett. 198:361-364(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 213-234.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Red velvet - Issue 86 of September 2007

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY706383 mRNA. Translation: AAU85387.1.
PIR	S01128.
RefSeq	NP_001005726.1. NM_001005726.1.
UniGene	Ssc.12241.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1214816.
STRING	9823.ENSSSCP00000004935.
Proteomic databases
PaxDb	P19620.
PRIDE	P19620.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	406192.
KEGG	ssc:406192.
Organism-specific databases
CTD	302.
Phylogenomic databases
eggNOG	NOG259189.
HOGENOM	HOG000158803.
HOVERGEN	HBG061815.
OrthoDB	EOG4KD6MM.
Family and domain databases
Gene3D	1.10.220.10. 4 hits.
InterPro	IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR002389. AnnexinII. [Graphical view]
PANTHER	PTHR10502. PTHR10502. 1 hit. PTHR10502:SF18. PTHR10502:SF18. 1 hit.
Pfam	PF00191. Annexin. 4 hits. [Graphical view]
PRINTS	PR00196. ANNEXIN. PR00198. ANNEXINII.
SMART	SM00335. ANX. 4 hits. [Graphical view]
SUPFAM	SSF47874. Annexin. 1 hit.
PROSITE	PS00223. ANNEXIN. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ANXA2_PIG

Accession

Primary (citable) accession number: P19620
Secondary accession number(s): Q5Y2C7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	May 29, 2007
Last modified:	April 3, 2013
This is version 115 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program