P19618 (NCPR_SALTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: NADPH--cytochrome P450 reductase Short name=CPR Short name=P450R EC=1.6.2.4
Organism	Salmo trutta (Brown trout)
Taxonomic identifier	8032 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonoidei › Salmonidae › Salmoninae › Salmo

Protein attributes

Sequence length	601 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Catalytic activity	NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.
Cofactor	FAD. FMN.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region.
Sequence similarities	In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH-hemoprotein reductase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 601	›601	NADPH--cytochrome P450 reductase		PRO_0000167601
Regions
	Domain	25 – 169	145	Flavodoxin-like
	Domain	224 – 425	202	FAD-binding FR-type
Experimental info
	Non-adjacent residues	426 – 427	2
	Non-adjacent residues	434 – 435	2
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P19618 [UniParc]. Last modified February 1, 1991. Version 1. Checksum: BC801767DE1D44C9	FASTA	601	68,305
	10 20 30 40 50 60 KLDQPAPSTQ ETSFIEKMKK TGRNIVVFYG SQTGTGEEFA NRLSKDAHRY GMGSMAADPE 70 80 90 100 110 120 EYDMSELSRL AEIGNSLAIF CMATYGEGDP TDNAQDFYDW LQETDGQLSG VNYPVFALGD 130 140 150 160 170 180 KTYEHYNAMG AYVDKRLEEL GAKRVFDLGM GDDDGNLEED FVTWREQFWP AMCEHFGVEA 190 200 210 220 230 240 SGEDSSVRQY ELKEHNDINM NKVYTGELGR LKSFETQKPP FDAKNPFLAP VTVNRKLNKA 250 260 270 280 290 300 GELHKMHLEV DITGSKIRYE SGDHVAVYPT NNTVIVNRLG QILGVDLDSV ISLNNLDEES 310 320 330 340 350 360 NKKHPFPCPT TYRTALTHYL DIIHPPRTNV LYELAQYATD LKDQENTDSM ASSAPEGKAL 370 380 390 400 410 420 YQSFVLEDNR NILAILEDLP SLRPPIDHLC ELMPRLQARY YSIASSSKVH PNSIHICAVL 430 440 450 460 470 480 VEYXTKGVAT TWLKYIRKSQ FRLPFKASNP VIMVGPGTGI APFMGFIQER GWLKESGKEV 490 500 510 520 530 540 GETVLYCGCR HKEEDYLYQE ELEQAHKKGA LTKLNVAFSR EQDQKVYVQH LLRKNKVDLW 550 560 570 580 590 600 RQIHEDYAHI YICGDARNMA RDVQTAFYEI AEELGGMTRT QATDYIKKLM TKGRYSQDVW S

References

[1]	"Structural comparison between the trout and mammalian hydrophilic domain of NADPH-cytochrome P-450 reductase." Urenjak J., Linder D., Lumper L. J. Chromatogr. A 397:123-136(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	A28577.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG000432.
Family and domain databases
Gene3D	1.20.990.10. 1 hit.
InterPro	IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR023173. NADPH_Cyt_P450_Rdtase_dom3. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCPR_SALTR

Accession

Primary (citable) accession number: P19618

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	February 1, 1991
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program