Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P19618

- NCPR_SALTR

UniProt

P19618 - NCPR_SALTR

Protein

NADPH--cytochrome P450 reductase

Gene
N/A
Organism
Salmo trutta (Brown trout)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Feb 1991)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

    Catalytic activityi

    NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

    Cofactori

    FAD.
    FMN.

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. NADPH-hemoprotein reductase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH--cytochrome P450 reductase (EC:1.6.2.4)
    Short name:
    CPR
    Short name:
    P450R
    OrganismiSalmo trutta (Brown trout)
    Taxonomic identifieri8032 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo

    Subcellular locationi

    Endoplasmic reticulum membrane; Peripheral membrane protein
    Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 601›601NADPH--cytochrome P450 reductasePRO_0000167601Add
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 169145Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini224 – 425202FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG000432.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragments.

    P19618-1 [UniParc]FASTAAdd to Basket

    « Hide

    KLDQPAPSTQ ETSFIEKMKK TGRNIVVFYG SQTGTGEEFA NRLSKDAHRY    50
    GMGSMAADPE EYDMSELSRL AEIGNSLAIF CMATYGEGDP TDNAQDFYDW 100
    LQETDGQLSG VNYPVFALGD KTYEHYNAMG AYVDKRLEEL GAKRVFDLGM 150
    GDDDGNLEED FVTWREQFWP AMCEHFGVEA SGEDSSVRQY ELKEHNDINM 200
    NKVYTGELGR LKSFETQKPP FDAKNPFLAP VTVNRKLNKA GELHKMHLEV 250
    DITGSKIRYE SGDHVAVYPT NNTVIVNRLG QILGVDLDSV ISLNNLDEES 300
    NKKHPFPCPT TYRTALTHYL DIIHPPRTNV LYELAQYATD LKDQENTDSM 350
    ASSAPEGKAL YQSFVLEDNR NILAILEDLP SLRPPIDHLC ELMPRLQARY 400
    YSIASSSKVH PNSIHICAVL VEYXTKGVAT TWLKYIRKSQ FRLPFKASNP 450
    VIMVGPGTGI APFMGFIQER GWLKESGKEV GETVLYCGCR HKEEDYLYQE 500
    ELEQAHKKGA LTKLNVAFSR EQDQKVYVQH LLRKNKVDLW RQIHEDYAHI 550
    YICGDARNMA RDVQTAFYEI AEELGGMTRT QATDYIKKLM TKGRYSQDVW 600
    S 601
    Length:601
    Mass (Da):68,305
    Last modified:February 1, 1991 - v1
    Checksum:iBC801767DE1D44C9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-adjacent residuesi426 – 4272Curated
    Non-adjacent residuesi434 – 4352Curated

    Sequence databases

    PIRiA28577.

    Cross-referencesi

    Sequence databases

    PIRi A28577.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG000432.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural comparison between the trout and mammalian hydrophilic domain of NADPH-cytochrome P-450 reductase."
      Urenjak J., Linder D., Lumper L.
      J. Chromatogr. A 397:123-136(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiNCPR_SALTR
    AccessioniPrimary (citable) accession number: P19618
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3