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Protein

Type-3 ice-structuring protein HPLC 12

Gene
N/A
Organism
Zoarces americanus (Ocean pout) (Macrozoarces americanus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei9Important for ice-binding1
Sitei14Important for ice-binding1
Sitei18Important for ice-binding1
Sitei44Important for ice-binding1

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antifreeze protein

Names & Taxonomyi

Protein namesi
Recommended name:
Type-3 ice-structuring protein HPLC 12
Alternative name(s):
Antifreeze protein QAE(HPLC 12)
ISP type III HPLC 12
OrganismiZoarces americanus (Ocean pout) (Macrozoarces americanus)
Taxonomic identifieri8199 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataEupercariaPerciformesCottioideiZoarcalesZoarcidaeZoarcinaeZoarces

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001551601 – 66Type-3 ice-structuring protein HPLC 12Add BLAST66

Expressioni

Tissue specificityi

Detected in blood serum (at protein level).1 Publication

Structurei

Secondary structure

166
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Helixi19 – 21Combined sources3
Beta strandi22 – 25Combined sources4
Helixi34 – 36Combined sources3
Helixi37 – 40Combined sources4
Beta strandi44 – 47Combined sources4
Helixi57 – 59Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AMEX-ray1.65A1-63[»]
1B7IX-ray1.65A1-65[»]
1B7JX-ray1.65A1-63[»]
1B7KX-ray2.50A1-63[»]
1EKLX-ray1.65A1-63[»]
1GZIX-ray1.80A1-63[»]
1HG7X-ray1.15A1-63[»]
1JABX-ray1.65A1-63[»]
1KDENMR-A1-63[»]
1KDFNMR-A1-63[»]
1MSIX-ray1.25A2-63[»]
1MSJX-ray2.30A1-63[»]
2AMEX-ray2.00A1-63[»]
2JIAX-ray1.60A1-65[»]
2MSIX-ray1.90A2-63[»]
2MSJX-ray1.90A1-63[»]
2SPGX-ray1.75A1-63[»]
3AMEX-ray2.30A1-63[»]
3MSIX-ray1.43A2-63[»]
3QF6neutron diffraction1.85A1-66[»]
4AMEX-ray2.05A1-63[»]
4MSIX-ray1.60A2-63[»]
4NY6Other1.85A1-63[»]
5C7RX-ray1.94A/B1-63[»]
5MSIX-ray1.60A2-63[»]
6AMEX-ray2.10A1-63[»]
6MSIX-ray1.65A2-63[»]
7AMEX-ray1.70A1-63[»]
7MSIX-ray1.70A2-63[»]
8AMEX-ray1.90A1-63[»]
8MSIX-ray2.60A1-63[»]
9AMEX-ray1.80A1-63[»]
9MSIX-ray2.60A1-63[»]
ProteinModelPortaliP19614.
SMRiP19614.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19614.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 63AFP-likePROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Belongs to the type-III AFP family.Curated
Contains 1 AFP-like domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG004237.

Family and domain databases

Gene3Di3.90.1210.10. 1 hit.
InterProiIPR006190. AFP_Neu5c_C.
IPR006013. Antifreeze_III.
[Graphical view]
PRINTSiPR00357. ANTIFREEZIII.
SUPFAMiSSF51269. SSF51269. 1 hit.
PROSITEiPS50844. AFP_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
NQASVVANQL IPINTALTLV MMRSEVVTPV GIPAEDIPRL VSMQVNRAVP
60
LGTTLMPDMV KGYPPA
Length:66
Mass (Da):7,027
Last modified:February 1, 1991 - v1
Checksum:iB6121A9902ED89F9
GO

Sequence databases

PIRiA30839.

Cross-referencesi

Sequence databases

PIRiA30839.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AMEX-ray1.65A1-63[»]
1B7IX-ray1.65A1-65[»]
1B7JX-ray1.65A1-63[»]
1B7KX-ray2.50A1-63[»]
1EKLX-ray1.65A1-63[»]
1GZIX-ray1.80A1-63[»]
1HG7X-ray1.15A1-63[»]
1JABX-ray1.65A1-63[»]
1KDENMR-A1-63[»]
1KDFNMR-A1-63[»]
1MSIX-ray1.25A2-63[»]
1MSJX-ray2.30A1-63[»]
2AMEX-ray2.00A1-63[»]
2JIAX-ray1.60A1-65[»]
2MSIX-ray1.90A2-63[»]
2MSJX-ray1.90A1-63[»]
2SPGX-ray1.75A1-63[»]
3AMEX-ray2.30A1-63[»]
3MSIX-ray1.43A2-63[»]
3QF6neutron diffraction1.85A1-66[»]
4AMEX-ray2.05A1-63[»]
4MSIX-ray1.60A2-63[»]
4NY6Other1.85A1-63[»]
5C7RX-ray1.94A/B1-63[»]
5MSIX-ray1.60A2-63[»]
6AMEX-ray2.10A1-63[»]
6MSIX-ray1.65A2-63[»]
7AMEX-ray1.70A1-63[»]
7MSIX-ray1.70A2-63[»]
8AMEX-ray1.90A1-63[»]
8MSIX-ray2.60A1-63[»]
9AMEX-ray1.80A1-63[»]
9MSIX-ray2.60A1-63[»]
ProteinModelPortaliP19614.
SMRiP19614.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG004237.

Miscellaneous databases

EvolutionaryTraceiP19614.

Family and domain databases

Gene3Di3.90.1210.10. 1 hit.
InterProiIPR006190. AFP_Neu5c_C.
IPR006013. Antifreeze_III.
[Graphical view]
PRINTSiPR00357. ANTIFREEZIII.
SUPFAMiSSF51269. SSF51269. 1 hit.
PROSITEiPS50844. AFP_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANP12_ZOAAM
AccessioniPrimary (citable) accession number: P19614
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.