P19595 (UGPA_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: UTP--glucose-1-phosphate uridylyltransferase EC=2.7.7.9 Alternative name(s): UDP-glucose pyrophosphorylase Short name=UDPGP Short name=UGPase
Organism	Solanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a central role as a glucosyl donor in cellular metabolic pathways.
Catalytic activity	UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.
Cofactor	Magnesium.
Enzyme regulation	Inhibition by uncomplexed, free UTP.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the UDPGP type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Nucleotidyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UTP:glucose-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 477	476	UTP--glucose-1-phosphate uridylyltransferase		PRO_0000185762
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.1
Natural variations
	Natural variant	5	1	T → A in strain: cv. Desiree, cv. ND860-2 and cv. Russet Burbank-1.
	Natural variant	30	1	E → D in strain: cv. Desiree, cv. ND860-2 and cv. Russet Burbank-1.
	Natural variant	82	1	K → N in strain: cv. Desiree, cv. ND860-2 and cv. Russet Burbank-1.
	Natural variant	445	1	K → E in strain: cv. Desiree, cv. ND860-2 and cv. Russet Burbank-1.
	Natural variant	450	1	V → I in strain: cv. Desiree, cv. ND860-2 and cv. Russet Burbank-1.
Experimental info
	Mutagenesis	263	1	K → Q: Significant decreased Vmax values. Ref.6
	Mutagenesis	329	1	K → Q: Increased Km, Vmax like wild-type. Ref.6
	Mutagenesis	367	1	K → Q: Almost complete loss of activity. Ref.6
	Mutagenesis	409	1	K → Q: Activity almost like wild-type. Ref.6
	Mutagenesis	410	1	K → Q: Activity almost like wild-type. Ref.6
	Sequence conflict	2	1	A → V in AAB71613. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P19595 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 060E8D08AAE22709	FASTA	477	51,874
	10 20 30 40 50 60 MATATTLSPA DAEKLNNLKS AVAGLNQISE NEKSGFINLV GRYLSGEAQH IDWSKIQTPT 70 80 90 100 110 120 DEVVVPYDKL APLSEDPAET KKLLDKLVVL KLNGGLGTTM GCTGPKSVIE VRNGLTFLDL 130 140 150 160 170 180 IVKQIEALNA KFGCSVPLLL MNSFNTHDDT LKIVEKYANS NIDIHTFNQS QYPRLVTEDF 190 200 210 220 230 240 APLPCKGNSG KDGWYPPGHG DVFPSLMNSG KLDALLAKGK EYVFVANSDN LGAIVDLKIL 250 260 270 280 290 300 NHLILNKNEY CMEVTPKTLA DVKGGTLISY EGKVQLLEIA QVPDEHVNEF KSIEKFKIFN 310 320 330 340 350 360 TNNLWVNLSA IKRLVEADAL KMEIIPNPKE VDGVKVLQLE TAAGAAIKFF DRAIGANVPR 370 380 390 400 410 420 SRFLPVKATS DLLLVQSDLY TLTDEGYVIR NPARSNPSNP SIELGPEFKK VANFLGRFKS 430 440 450 460 470 IPSIIDLDSL KVTGDVWFGS GVTLKGKVTV AAKSGVKLEI PDGAVIANKD INGPEDI

References

[1]	"UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencing." Katsube T., Kazuta Y., Mori H., Nakano K., Tanizawa K., Fukui T. J. Biochem. 108:321-326(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ACETYLATION AT ALA-2. Tissue: Tuber.
[2]	"Organization and transcription of the gene encoding potato UDP-glucose pyrophosphorylase." Borovkov A.Y., McClean P.E., Secor G.A. Gene 186:293-297(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Lemhi Russet.
[3]	"UDP-glucose pyrophosphorylase of potato tuber: cDNA sequence, transgenic tuber-specific inhibition and control of post-harvest sugar metabolism." Spychalla J.P., Bevan M.W. Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Desiree. Tissue: Tuber.
[4]	"Molecular cloning and sequence variation of UDP-glucose pyrophosphorylase cDNAs from potatoes sensitive and resistant to cold sweetening." Sowokinos J.R., Vigdorovich V., Abrahamsen M. J. Plant Physiol. 161:947-955(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. ND860-2 and cv. Russet Burbank-1.
[5]	"UDP-glucose pyrophosphorylase from potato tuber: purification and characterization." Nakano K., Omura Y., Tagaya M., Fukui T. J. Biochem. 106:528-532(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Tissue: Tuber.
[6]	"Expression in Escherichia coli of UDP-glucose pyrophosphorylase cDNA from potato tuber and functional assessment of the five lysyl residues located at the substrate-binding site." Katsube T., Kazuta Y., Tanizawa K., Fukui T. Biochemistry 30:8546-8551(1991) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF LYSINE RESIDUES.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00667 mRNA. Translation: BAA00570.1. U20345 Genomic DNA. Translation: AAB71613.1. Z18924 mRNA. Translation: CAA79357.1. AY082618 mRNA. Translation: AAL99193.1. AY082619 mRNA. Translation: AAL99194.1.
PIR	XNPOU. JX0128. S31431.
3D structure databases
ProteinModelPortal	P19595.
SMR	P19595. Positions 15-477.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002618. UDPGP_trans. IPR016267. UDPGP_trans_subgr. [Graphical view]
PANTHER	PTHR11952. PTHR11952. 1 hit. PTHR11952:SF1. PTHR11952:SF1. 1 hit.
Pfam	PF01704. UDPGP. 1 hit. [Graphical view]
PIRSF	PIRSF000806. UDPGP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UGPA_SOLTU

Accession

Primary (citable) accession number: P19595
Secondary accession number(s): Q43192, Q5F1U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program