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P19588

- LEC5_VIGUC

UniProt

P19588 - LEC5_VIGUC

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Protein

Lectin DB58

Gene
N/A
Organism
Vigna unguiculata subsp. cylindrica (Horse gram) (Dolichos biflorus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Metalloglycoprotein, containing Ca, Mg, Mn, and Zn and the carbohydrates galactose, glucosamine, mannose, and fucose. It agglutinates erythrocytes of blood group A1.

GO - Molecular functioni

  1. mannose binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Calcium, Lectin, Mannose-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lectin DB58
Cleaved into the following 2 chains:
OrganismiVigna unguiculata subsp. cylindrica (Horse gram) (Dolichos biflorus)
Taxonomic identifieri3840 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Add
BLAST
Chaini23 – 275253Lectin DB58 subunit alphaPRO_0000017613Add
BLAST
Chaini23 – 264242Lectin DB58 subunit betaPRO_0000017614Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...)
Glycosylationi101 – 1011N-linked (GlcNAc...)

Post-translational modificationi

Leu-264 is missing in a major portion of the beta subunit, suggesting an origin by sequential removal of amino acids rather than a processing by endoproteolytic cleavage.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Heterodimer, composed of an alpha and a beta subunit derived from a single precursor.

Structurei

Secondary structure

1
275
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 318
Beta strandi38 – 425
Beta strandi48 – 525
Beta strandi67 – 748
Turni81 – 833
Beta strandi88 – 969
Helixi102 – 1043
Beta strandi108 – 1158
Turni127 – 1293
Helixi137 – 1393
Beta strandi142 – 1476
Beta strandi160 – 16910
Beta strandi171 – 1755
Beta strandi182 – 19110
Turni192 – 1954
Beta strandi196 – 2038
Helixi204 – 2063
Beta strandi208 – 2158
Helixi218 – 2214
Beta strandi224 – 23310
Beta strandi245 – 25511

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G7YX-ray2.50A/B/C/D/E/F23-275[»]
1LULX-ray3.30A/B/C/D/E/F23-275[»]
ProteinModelPortaliP19588.
SMRiP19588. Positions 23-275.

Miscellaneous databases

EvolutionaryTraceiP19588.

Family & Domainsi

Sequence similaritiesi

Belongs to the leguminous lectin family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR016363. Lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19588-1 [UniParc]FASTAAdd to Basket

« Hide

MASSTVSVVL SLFLLLLTQA YSADIQSFSF KNFNSSSFIL QGDATVSSSK    50
LRLTKVKGNG LPTLSSLGRA FYSSPIQIYD KSTGAVASWA TSFTANIFAP 100
NKSSSADGIA FALVPVGSEP KSNSGFLGVF DSDVYDNSAQ TVAVEFDTFS 150
NTDWDPTSRH IGIDVNSIKS IRTASWGLAN GQNAEILITY NAATSLLVAS 200
LVHPSRRTSY IVSERVDITN ELPEYVSIGF SATTGLSEGY TETHDVLSWS 250
FASKLPDDST TEPLDIASYL VRNVL 275
Length:275
Mass (Da):29,453
Last modified:July 15, 1999 - v2
Checksum:i53D72ACB02EAE03F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti236 – 2372LS → FF in AAA33142. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23216 mRNA. Translation: AAA33142.1.
M34271 Genomic DNA. Translation: AAA33140.1.
PIRiA31972.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23216 mRNA. Translation: AAA33142.1 .
M34271 Genomic DNA. Translation: AAA33140.1 .
PIRi A31972.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G7Y X-ray 2.50 A/B/C/D/E/F 23-275 [» ]
1LUL X-ray 3.30 A/B/C/D/E/F 23-275 [» ]
ProteinModelPortali P19588.
SMRi P19588. Positions 23-275.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P19588.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR016363. Lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view ]
Pfami PF00139. Lectin_legB. 1 hit.
[Graphical view ]
PIRSFi PIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning, primary structure, and in vitro biosynthesis of the DB58 lectin from Dolichos biflorus."
    Schnell D.J., Etzler M.E.
    J. Biol. Chem. 263:14648-14653(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Two lectin genes differentially expressed in Dolichos biflorus differ primarily by a 116-base pair sequence in their 5' flanking regions."
    Harada J.J., Spadoro-Tank J., Maxwell J.C., Schnell D.J., Etzler M.E.
    J. Biol. Chem. 265:4997-5001(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Isolation and characterization of subunits of DB58, a lectin from the stems and leaves of Dolichos biflorus."
    Etzler M.E.
    Biochemistry 33:9778-9783(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 255-275, PROTEOLYTIC PROCESSING.
    Tissue: Leaf and Stem.
  4. "Carbohydrate binding, quaternary structure and a novel hydrophobic binding site in two legume lectin oligomers from Dolichos biflorus."
    Hamelryck T.W., Loris R., Bouckaert J., Dao-Thi M.-H., Strecker G., Imberty A., Fernandez E., Wyns L., Etzler M.E.
    J. Mol. Biol. 286:1161-1177(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS).

Entry informationi

Entry nameiLEC5_VIGUC
AccessioniPrimary (citable) accession number: P19588
Secondary accession number(s): Q39665
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 15, 1999
Last modified: February 19, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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