P19588 (LEC5_DOLBI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lectin DB58 Cleaved into the following 2 chains: Lectin DB58 subunit alpha Lectin DB58 subunit beta
Organism	Dolichos biflorus (Horse gram)
Taxonomic identifier	3840 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna › Vigna unguiculata

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metalloglycoprotein, containing Ca, Mg, Mn, and Zn and the carbohydrates galactose, glucosamine, mannose, and fucose. It agglutinates erythrocytes of blood group A1.
Subunit structure	Heterodimer, composed of an alpha and a beta subunit derived from a single precursor.
Post-translational modification	Leu-264 is missing in a major portion of the beta subunit, suggesting an origin by sequential removal of amino acids rather than a processing by endoproteolytic cleavage.
Sequence similarities	Belongs to the leguminous lectin family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Lectin Mannose-binding
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	mannose binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Chain

23 – 275

253

Lectin DB58 subunit alpha

PRO_0000017613

Chain

23 – 264

242

Lectin DB58 subunit beta

PRO_0000017614

Amino acid modifications

Glycosylation

34

1

N-linked (GlcNAc...)

Glycosylation

101

1

N-linked (GlcNAc...)

Experimental info

Sequence conflict

236 – 237

2

LS → FF in AAA33142. Ref.1

Secondary structure

1

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275

Helix Strand Turn

Details...

Beta strand

24 – 31

8

Beta strand

38 – 42

5

Beta strand

48 – 52

5

Beta strand

67 – 74

8

Turn

81 – 83

3

Beta strand

88 – 96

9

Helix

102 – 104

3

Beta strand

108 – 115

8

Turn

127 – 129

3

Helix

137 – 139

3

Beta strand

142 – 147

6

Beta strand

160 – 169

10

Beta strand

171 – 175

5

Beta strand

182 – 191

10

Turn

192 – 195

4

Beta strand

196 – 203

8

Helix

204 – 206

3

Beta strand

208 – 215

8

Helix

218 – 221

4

Beta strand

224 – 233

10

Beta strand

245 – 255

11

Sequences

	Sequence		Length	Mass (Da)
	P19588 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 53D72ACB02EAE03F	FASTA	275	29,453
	10 20 30 40 50 60 MASSTVSVVL SLFLLLLTQA YSADIQSFSF KNFNSSSFIL QGDATVSSSK LRLTKVKGNG 70 80 90 100 110 120 LPTLSSLGRA FYSSPIQIYD KSTGAVASWA TSFTANIFAP NKSSSADGIA FALVPVGSEP 130 140 150 160 170 180 KSNSGFLGVF DSDVYDNSAQ TVAVEFDTFS NTDWDPTSRH IGIDVNSIKS IRTASWGLAN 190 200 210 220 230 240 GQNAEILITY NAATSLLVAS LVHPSRRTSY IVSERVDITN ELPEYVSIGF SATTGLSEGY 250 260 270 TETHDVLSWS FASKLPDDST TEPLDIASYL VRNVL

References

[1]	"cDNA cloning, primary structure, and in vitro biosynthesis of the DB58 lectin from Dolichos biflorus." Schnell D.J., Etzler M.E. J. Biol. Chem. 263:14648-14653(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Two lectin genes differentially expressed in Dolichos biflorus differ primarily by a 116-base pair sequence in their 5' flanking regions." Harada J.J., Spadoro-Tank J., Maxwell J.C., Schnell D.J., Etzler M.E. J. Biol. Chem. 265:4997-5001(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Isolation and characterization of subunits of DB58, a lectin from the stems and leaves of Dolichos biflorus." Etzler M.E. Biochemistry 33:9778-9783(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 255-275, PROTEOLYTIC PROCESSING. Tissue: Leaf and Stem.
[4]	"Carbohydrate binding, quaternary structure and a novel hydrophobic binding site in two legume lectin oligomers from Dolichos biflorus." Hamelryck T.W., Loris R., Bouckaert J., Dao-Thi M.-H., Strecker G., Imberty A., Fernandez E., Wyns L., Etzler M.E. J. Mol. Biol. 286:1161-1177(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M23216 mRNA. Translation: AAA33142.1.
M34271 Genomic DNA. Translation: AAA33140.1.

PIR

A31972.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G7Y	X-ray	2.50	A/B/C/D/E/F	23-275	[»]
1LUL	X-ray	3.30	A/B/C/D/E/F	23-275	[»]

ProteinModelPortal

P19588.

SMR

P19588. Positions 23-275.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.60.120.200. 1 hit.

InterPro

IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR016363. Lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]

Pfam

PF00139. Lectin_legB. 1 hit.
[Graphical view]

PIRSF

PIRSF002690. L-type_lectin_plant. 1 hit.

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

PROSITE

PS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P19588.

Entry information

Entry name

LEC5_DOLBI

Accession

Primary (citable) accession number: P19588
Secondary accession number(s): Q39665

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	July 15, 1999
Last modified:	April 3, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program