Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P19541

- RDS2_YEAST

UniProt

P19541 - RDS2_YEAST

Protein

Regulator of drug sensitivity 2

Gene

RDS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcription factor which regulates the expression of genes for gluconeogenesis, the TCA cycle, and glucose metabolism. Involved in the cell wall remodeling process and drug resistance.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi15 – 4531Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
    3. signal transducer activity Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to xenobiotic stimulus Source: SGD
    2. negative regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source Source: SGD
    3. positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter Source: SGD
    4. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    5. positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source Source: SGD

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34032-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulator of drug sensitivity 2
    Gene namesi
    Name:RDS2
    Ordered Locus Names:YPL133C
    ORF Names:LPI12C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL133c.
    SGDiS000006054. RDS2.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Sensitivity to ketoconazole.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 446446Regulator of drug sensitivity 2PRO_0000115006Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei102 – 1021Phosphoserine2 Publications
    Modified residuei231 – 2311Phosphothreonine2 Publications

    Post-translational modificationi

    Phosphorylated by SNF1 in absence of glucose. The phosphorylation is required for induction of transcription of gluconeogenic genes.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP19541.
    PaxDbiP19541.
    PeptideAtlasiP19541.

    Expressioni

    Gene expression databases

    GenevestigatoriP19541.

    Interactioni

    Protein-protein interaction databases

    BioGridi36048. 32 interactions.
    DIPiDIP-1515N.
    IntActiP19541. 5 interactions.
    MINTiMINT-400287.
    STRINGi4932.YPL133C.

    Structurei

    3D structure databases

    ProteinModelPortaliP19541.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG44741.
    GeneTreeiENSGT00530000068325.
    HOGENOMiHOG000164620.
    OMAiGNSNANQ.
    OrthoDBiEOG7647BW.

    Family and domain databases

    Gene3Di4.10.240.10. 1 hit.
    InterProiIPR000014. PAS.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view]
    SMARTiSM00066. GAL4. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF55785. SSF55785. 1 hit.
    SSF57701. SSF57701. 1 hit.
    PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19541-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSANSGVKRA SKAFKTCLFC KRSHVVCDKQ RPCSRCVKRD IAHLCREDDI    50
    AVPNEMPSQH ESSPNDNNIQ GKYANKAHTG IPSDYQNEPV NKSGSTYGEE 100
    LSPKLDSSLV NDTTSLLLPQ QPVFVSENVG SEFSSLNEFL SMLENPLLTQ 150
    TSLSSSSASN VHLENGSQTT QSPLEYQNDN RRDEIGVARQ ENRSPTIMSG 200
    SSNSISKGDK QDQEKEESRI LANANENSAP TPKEQFFLTA ADPSTEMTPE 250
    HRLKLVINAK LEAGLLKPYN YAKGYARLQD YMDKYMNQSS KQRILKPLST 300
    IRPAFRTIAR SLKDVDLVLV EESFERMLLS YDRVFTSMSM PACLCRRTGE 350
    IYRANKEFAS LVDCTVDDLR DGKLAIYELM TEESAVNFWE KYGSIAFDKG 400
    QKAVLTSCSL RTKDGIRKRP CCFSFTIRRD RYNIPICIVG NFIPLS 446
    Length:446
    Mass (Da):50,082
    Last modified:October 1, 1996 - v2
    Checksum:iC78D3632DDCC3EA0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43703 Genomic DNA. Translation: AAB68226.1.
    AY693168 Genomic DNA. Translation: AAT93187.1.
    X55731 Genomic DNA. Translation: CAA39262.1.
    BK006949 Genomic DNA. Translation: DAA11300.1.
    PIRiS69051.
    RefSeqiNP_015192.1. NM_001183947.1.

    Genome annotation databases

    EnsemblFungiiYPL133C; YPL133C; YPL133C.
    GeneIDi855970.
    KEGGisce:YPL133C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43703 Genomic DNA. Translation: AAB68226.1 .
    AY693168 Genomic DNA. Translation: AAT93187.1 .
    X55731 Genomic DNA. Translation: CAA39262.1 .
    BK006949 Genomic DNA. Translation: DAA11300.1 .
    PIRi S69051.
    RefSeqi NP_015192.1. NM_001183947.1.

    3D structure databases

    ProteinModelPortali P19541.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36048. 32 interactions.
    DIPi DIP-1515N.
    IntActi P19541. 5 interactions.
    MINTi MINT-400287.
    STRINGi 4932.YPL133C.

    Proteomic databases

    MaxQBi P19541.
    PaxDbi P19541.
    PeptideAtlasi P19541.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL133C ; YPL133C ; YPL133C .
    GeneIDi 855970.
    KEGGi sce:YPL133C.

    Organism-specific databases

    CYGDi YPL133c.
    SGDi S000006054. RDS2.

    Phylogenomic databases

    eggNOGi NOG44741.
    GeneTreei ENSGT00530000068325.
    HOGENOMi HOG000164620.
    OMAi GNSNANQ.
    OrthoDBi EOG7647BW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34032-MONOMER.

    Miscellaneous databases

    NextBioi 980783.

    Gene expression databases

    Genevestigatori P19541.

    Family and domain databases

    Gene3Di 4.10.240.10. 1 hit.
    InterProi IPR000014. PAS.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view ]
    SMARTi SM00066. GAL4. 1 hit.
    SM00091. PAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55785. SSF55785. 1 hit.
    SSF57701. SSF57701. 1 hit.
    PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Cytochrome oxidase assembly in yeast requires the product of COX11, a homolog of the P. denitrificans protein encoded by ORF3."
      Tzagoloff A., Capitanio N., Nobrega M.P., Gatti D.
      EMBO J. 9:2759-2764(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95.
    5. "Comprehensive sequence analysis of the 182 predicted open reading frames of yeast chromosome III."
      Bork P., Ouzounis C., Sander C., Scharf M., Schneider R., Sonnhammer E.
      Protein Sci. 1:1677-1690(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRESENCE OF A ZN(2)-CYS(6) FUNGAL-TYPE BINUCLEAR CLUSTER.
    6. "New regulators of drug sensitivity in the family of yeast zinc cluster proteins."
      Akache B., Turcotte B.
      J. Biol. Chem. 277:21254-21260(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A drug-sensitive genetic network masks fungi from the immune system."
      Wheeler R.T., Fink G.R.
      PLoS Pathog. 2:E35-E35(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Linking DNA-binding proteins to their recognition sequences by using protein microarrays."
      Ho S.-W., Jona G., Chen C.T.L., Johnston M., Snyder M.
      Proc. Natl. Acad. Sci. U.S.A. 103:9940-9945(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING.
    11. "Regulation of gluconeogenesis in Saccharomyces cerevisiae is mediated by activator and repressor functions of Rds2."
      Soontorngun N., Larochelle M., Drouin S., Robert F., Turcotte B.
      Mol. Cell. Biol. 27:7895-7905(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, PHOSPHORYLATION BY SNF1.
    12. "Saccharomyces cerevisiae Rds2 transcription factor involvement in cell wall composition and architecture."
      Moreno I., Tutrone N., Sentandreu R., Valentin E.
      Int. Microbiol. 11:57-63(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102 AND THR-231, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRDS2_YEAST
    AccessioniPrimary (citable) accession number: P19541
    Secondary accession number(s): D6W3N4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 799 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3