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P19541 (RDS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of drug sensitivity 2
Gene names
Name:RDS2
Ordered Locus Names:YPL133C
ORF Names:LPI12C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor which regulates the expression of genes for gluconeogenesis, the TCA cycle, and glucose metabolism. Involved in the cell wall remodeling process and drug resistance. Ref.9 Ref.11 Ref.12

Subcellular location

Cytoplasm. Nucleus Ref.7.

Post-translational modification

Phosphorylated by SNF1 in absence of glucose. The phosphorylation is required for induction of transcription of gluconeogenic genes. Ref.11

Disruption phenotype

Sensitivity to ketoconazole. Ref.6

Miscellaneous

Present with 799 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   LigandDNA-binding
Metal-binding
Zinc
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to xenobiotic stimulus

Inferred from mutant phenotype Ref.6. Source: SGD

negative regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source

Inferred from mutant phenotype Ref.11. Source: SGD

positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype Ref.11. Source: SGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype Ref.6. Source: SGD

positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source

Inferred from mutant phenotype Ref.11. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred by curator Ref.11. Source: SGD

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from direct assay Ref.11. Source: SGD

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from mutant phenotype Ref.11. Source: SGD

signal transducer activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Regulator of drug sensitivity 2
PRO_0000115006

Regions

DNA binding15 – 4531Zn(2)-C6 fungal-type Ref.10 Ref.11

Amino acid modifications

Modified residue1021Phosphoserine Ref.13
Modified residue2311Phosphothreonine Ref.13

Sequences

Sequence LengthMass (Da)Tools
P19541 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: C78D3632DDCC3EA0

FASTA44650,082
        10         20         30         40         50         60 
MSANSGVKRA SKAFKTCLFC KRSHVVCDKQ RPCSRCVKRD IAHLCREDDI AVPNEMPSQH 

        70         80         90        100        110        120 
ESSPNDNNIQ GKYANKAHTG IPSDYQNEPV NKSGSTYGEE LSPKLDSSLV NDTTSLLLPQ 

       130        140        150        160        170        180 
QPVFVSENVG SEFSSLNEFL SMLENPLLTQ TSLSSSSASN VHLENGSQTT QSPLEYQNDN 

       190        200        210        220        230        240 
RRDEIGVARQ ENRSPTIMSG SSNSISKGDK QDQEKEESRI LANANENSAP TPKEQFFLTA 

       250        260        270        280        290        300 
ADPSTEMTPE HRLKLVINAK LEAGLLKPYN YAKGYARLQD YMDKYMNQSS KQRILKPLST 

       310        320        330        340        350        360 
IRPAFRTIAR SLKDVDLVLV EESFERMLLS YDRVFTSMSM PACLCRRTGE IYRANKEFAS 

       370        380        390        400        410        420 
LVDCTVDDLR DGKLAIYELM TEESAVNFWE KYGSIAFDKG QKAVLTSCSL RTKDGIRKRP 

       430        440 
CCFSFTIRRD RYNIPICIVG NFIPLS 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Cytochrome oxidase assembly in yeast requires the product of COX11, a homolog of the P. denitrificans protein encoded by ORF3."
Tzagoloff A., Capitanio N., Nobrega M.P., Gatti D.
EMBO J. 9:2759-2764(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95.
[5]"Comprehensive sequence analysis of the 182 predicted open reading frames of yeast chromosome III."
Bork P., Ouzounis C., Sander C., Scharf M., Schneider R., Sonnhammer E.
Protein Sci. 1:1677-1690(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PRESENCE OF A ZN(2)-CYS(6) FUNGAL-TYPE BINUCLEAR CLUSTER.
[6]"New regulators of drug sensitivity in the family of yeast zinc cluster proteins."
Akache B., Turcotte B.
J. Biol. Chem. 277:21254-21260(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A drug-sensitive genetic network masks fungi from the immune system."
Wheeler R.T., Fink G.R.
PLoS Pathog. 2:E35-E35(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Linking DNA-binding proteins to their recognition sequences by using protein microarrays."
Ho S.-W., Jona G., Chen C.T.L., Johnston M., Snyder M.
Proc. Natl. Acad. Sci. U.S.A. 103:9940-9945(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING.
[11]"Regulation of gluconeogenesis in Saccharomyces cerevisiae is mediated by activator and repressor functions of Rds2."
Soontorngun N., Larochelle M., Drouin S., Robert F., Turcotte B.
Mol. Cell. Biol. 27:7895-7905(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, PHOSPHORYLATION BY SNF1.
[12]"Saccharomyces cerevisiae Rds2 transcription factor involvement in cell wall composition and architecture."
Moreno I., Tutrone N., Sentandreu R., Valentin E.
Int. Microbiol. 11:57-63(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102 AND THR-231, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43703 Genomic DNA. Translation: AAB68226.1.
AY693168 Genomic DNA. Translation: AAT93187.1.
X55731 Genomic DNA. Translation: CAA39262.1.
BK006949 Genomic DNA. Translation: DAA11300.1.
PIRS69051.
RefSeqNP_015192.1. NM_001183947.1.

3D structure databases

ProteinModelPortalP19541.
SMRP19541. Positions 13-51.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36048. 32 interactions.
DIPDIP-1515N.
IntActP19541. 5 interactions.
MINTMINT-400287.
STRING4932.YPL133C.

Proteomic databases

PaxDbP19541.
PeptideAtlasP19541.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL133C; YPL133C; YPL133C.
GeneID855970.
KEGGsce:YPL133C.

Organism-specific databases

CYGDYPL133c.
SGDS000006054. RDS2.

Phylogenomic databases

eggNOGNOG44741.
GeneTreeENSGT00530000068325.
HOGENOMHOG000164620.
OMAGNSNANQ.
OrthoDBEOG7647BW.

Enzyme and pathway databases

BioCycYEAST:G3O-34032-MONOMER.

Gene expression databases

GenevestigatorP19541.

Family and domain databases

Gene3D4.10.240.10. 1 hit.
InterProIPR000014. PAS.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
SMARTSM00066. GAL4. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF55785. SSF55785. 1 hit.
SSF57701. SSF57701. 1 hit.
PROSITEPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980783.

Entry information

Entry nameRDS2_YEAST
AccessionPrimary (citable) accession number: P19541
Secondary accession number(s): D6W3N4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families