P19541 (RDS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulator of drug sensitivity 2

Gene names

Name:	RDS2
Ordered Locus Names:	YPL133C
ORF Names:	LPI12C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor which regulates the expression of genes for gluconeogenesis, the TCA cycle, and glucose metabolism. Involved in the cell wall remodeling process and drug resistance. Ref.9 Ref.11 Ref.12
Subcellular location	Cytoplasm. Nucleus Ref.7.
Post-translational modification	Phosphorylated by SNF1 in absence of glucose. The phosphorylation is required for induction of transcription of gluconeogenic genes. Ref.11
Disruption phenotype	Sensitivity to ketoconazole. Ref.6
Miscellaneous	Present with 799 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to xenobiotic stimulus Inferred from mutant phenotype Ref.6. Source: SGD negative regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source Inferred from mutant phenotype Ref.11. Source: SGD positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.11. Source: SGD positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source Inferred from mutant phenotype Ref.11. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.7. Source: SGD nucleus Inferred from direct assay Ref.7. Source: SGD
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay Ref.11. Source: SGD RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from mutant phenotype Ref.11. Source: SGD signal transducer activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Regulator of drug sensitivity 2		PRO_0000115006
Regions
	DNA binding	15 – 45	31	Zn(2)-C6 fungal-type Ref.10 Ref.11
Amino acid modifications
	Modified residue	102	1	Phosphoserine Ref.13
	Modified residue	231	1	Phosphothreonine Ref.13

Sequences

	Sequence		Length	Mass (Da)
	P19541 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: C78D3632DDCC3EA0	FASTA	446	50,082
	10 20 30 40 50 60 MSANSGVKRA SKAFKTCLFC KRSHVVCDKQ RPCSRCVKRD IAHLCREDDI AVPNEMPSQH 70 80 90 100 110 120 ESSPNDNNIQ GKYANKAHTG IPSDYQNEPV NKSGSTYGEE LSPKLDSSLV NDTTSLLLPQ 130 140 150 160 170 180 QPVFVSENVG SEFSSLNEFL SMLENPLLTQ TSLSSSSASN VHLENGSQTT QSPLEYQNDN 190 200 210 220 230 240 RRDEIGVARQ ENRSPTIMSG SSNSISKGDK QDQEKEESRI LANANENSAP TPKEQFFLTA 250 260 270 280 290 300 ADPSTEMTPE HRLKLVINAK LEAGLLKPYN YAKGYARLQD YMDKYMNQSS KQRILKPLST 310 320 330 340 350 360 IRPAFRTIAR SLKDVDLVLV EESFERMLLS YDRVFTSMSM PACLCRRTGE IYRANKEFAS 370 380 390 400 410 420 LVDCTVDDLR DGKLAIYELM TEESAVNFWE KYGSIAFDKG QKAVLTSCSL RTKDGIRKRP 430 440 CCFSFTIRRD RYNIPICIVG NFIPLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Cytochrome oxidase assembly in yeast requires the product of COX11, a homolog of the P. denitrificans protein encoded by ORF3." Tzagoloff A., Capitanio N., Nobrega M.P., Gatti D. EMBO J. 9:2759-2764(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95.
[5]	"Comprehensive sequence analysis of the 182 predicted open reading frames of yeast chromosome III." Bork P., Ouzounis C., Sander C., Scharf M., Schneider R., Sonnhammer E. Protein Sci. 1:1677-1690(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PRESENCE OF A ZN(2)-CYS(6) FUNGAL-TYPE BINUCLEAR CLUSTER.
[6]	"New regulators of drug sensitivity in the family of yeast zinc cluster proteins." Akache B., Turcotte B. J. Biol. Chem. 277:21254-21260(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A drug-sensitive genetic network masks fungi from the immune system." Wheeler R.T., Fink G.R. PLoS Pathog. 2:E35-E35(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Linking DNA-binding proteins to their recognition sequences by using protein microarrays." Ho S.-W., Jona G., Chen C.T.L., Johnston M., Snyder M. Proc. Natl. Acad. Sci. U.S.A. 103:9940-9945(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING.
[11]	"Regulation of gluconeogenesis in Saccharomyces cerevisiae is mediated by activator and repressor functions of Rds2." Soontorngun N., Larochelle M., Drouin S., Robert F., Turcotte B. Mol. Cell. Biol. 27:7895-7905(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DNA-BINDING, PHOSPHORYLATION BY SNF1.
[12]	"Saccharomyces cerevisiae Rds2 transcription factor involvement in cell wall composition and architecture." Moreno I., Tutrone N., Sentandreu R., Valentin E. Int. Microbiol. 11:57-63(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102 AND THR-231, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U43703 Genomic DNA. Translation: AAB68226.1. AY693168 Genomic DNA. Translation: AAT93187.1. X55731 Genomic DNA. Translation: CAA39262.1. BK006949 Genomic DNA. Translation: DAA11300.1.
PIR	S69051.
RefSeq	NP_015192.1. NM_001183947.1.
3D structure databases
ProteinModelPortal	P19541.
SMR	P19541. Positions 13-50.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1515N.
IntAct	P19541. 5 interactions.
MINT	MINT-400287.
STRING	4932.YPL133C.
Proteomic databases
PaxDb	P19541.
PeptideAtlas	P19541.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL133C; YPL133C; YPL133C.
GeneID	855970.
KEGG	sce:YPL133C.
Organism-specific databases
CYGD	YPL133c.
SGD	S000006054. RDS2.
Phylogenomic databases
eggNOG	NOG44741.
GeneTree	ENSGT00530000068325.
HOGENOM	HOG000164620.
OMA	LMTEESA.
OrthoDB	EOG44BF9X.
Gene expression databases
Genevestigator	P19541.
GermOnline	YPL133C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	4.10.240.10. 1 hit.
InterPro	IPR000014. PAS. IPR001138. Zn2-C6_fun-type_DNA-bd. [Graphical view]
SMART	SM00066. GAL4. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
SUPFAM	SSF57701. Fungi_TrN. 1 hit.
PROSITE	PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980783.

Entry information

Entry name

RDS2_YEAST

Accession

Primary (citable) accession number: P19541
Secondary accession number(s): D6W3N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 121 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program