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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53Glyceraldehyde 3-phosphateBy similarity1
Active sitei95Proton donorBy similarity1
Metal bindingi96Zinc 1; catalyticBy similarity1
Metal bindingi131Zinc 2By similarity1
Metal bindingi161Zinc 2By similarity1
Metal bindingi212Zinc 1; catalyticBy similarity1
Binding sitei213Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi252Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCORYNE:G18NG-12387-MONOMER.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
Synonyms:fda
Ordered Locus Names:Cgl2770, cg3068
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001787122 – 344Fructose-bisphosphate aldolaseAdd BLAST343

Proteomic databases

PRIDEiP19537.

2D gel databases

World-2DPAGE0001:P19537.

Interactioni

Protein-protein interaction databases

STRINGi196627.cg3068.

Structurei

3D structure databases

ProteinModelPortaliP19537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni253 – 255Dihydroxyacetone phosphate bindingBy similarity3
Regioni274 – 277Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107RCW. Bacteria.
COG0191. LUCA.
HOGENOMiHOG000227794.
KOiK01624.
OMAiRMSKMGM.

Family and domain databases

CDDicd00946. FBP_aldolase_IIA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR006411. Fruct_bisP_bact.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19537-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIATPEVYN EMLDRAKEGG FAFPAINCTS SETINAALKG FAEAESDGII
60 70 80 90 100
QFSTGGAEFG SGLAVKNKVK GAVALAAFAH EAAKSYGINV ALHTDHCQKE
110 120 130 140 150
VLDEYVRPLL AISQERVDRG ELPLFQSHMW DGSAVPIDEN LEIAQELLAK
160 170 180 190 200
AKAANIILEV EIGVVGGEED GVEAKAGANL YTSPEDFEKT IDAIGTGEKG
210 220 230 240 250
RYLLAATFGN VHGVYKPGNV KLRPEVLLEG QQVARKKLGL ADDALPFDFV
260 270 280 290 300
FHGGSGSEKE KIEEALTYGV IKMNVDTDTQ YAFTRPIVSH MFENYNGVLK
310 320 330 340
IDGEVGNKKA YDPRSYMKKA EQSMSERIIE SCQDLKSVGK TTSK
Length:344
Mass (Da):37,214
Last modified:January 23, 2007 - v3
Checksum:i745B189A2AC72BBF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti280Q → H in CAA35190 (PubMed:2615658).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17313 Genomic DNA. Translation: CAA35190.1.
BA000036 Genomic DNA. Translation: BAC00164.1.
BX927156 Genomic DNA. Translation: CAF20791.1.
PIRiS09283.
RefSeqiNP_601964.1. NC_003450.3.
WP_011015363.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAC00164; BAC00164; BAC00164.
CAF20791; CAF20791; cg3068.
GeneIDi1020714.
KEGGicgb:cg3068.
cgl:NCgl2673.
PATRICi21497578. VBICorGlu203724_2701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17313 Genomic DNA. Translation: CAA35190.1.
BA000036 Genomic DNA. Translation: BAC00164.1.
BX927156 Genomic DNA. Translation: CAF20791.1.
PIRiS09283.
RefSeqiNP_601964.1. NC_003450.3.
WP_011015363.1. NC_006958.1.

3D structure databases

ProteinModelPortaliP19537.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg3068.

2D gel databases

World-2DPAGE0001:P19537.

Proteomic databases

PRIDEiP19537.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC00164; BAC00164; BAC00164.
CAF20791; CAF20791; cg3068.
GeneIDi1020714.
KEGGicgb:cg3068.
cgl:NCgl2673.
PATRICi21497578. VBICorGlu203724_2701.

Phylogenomic databases

eggNOGiENOG4107RCW. Bacteria.
COG0191. LUCA.
HOGENOMiHOG000227794.
KOiK01624.
OMAiRMSKMGM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciCORYNE:G18NG-12387-MONOMER.

Family and domain databases

CDDicd00946. FBP_aldolase_IIA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR006411. Fruct_bisP_bact.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALF_CORGL
AccessioniPrimary (citable) accession number: P19537
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.