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P19537

- ALF_CORGL

UniProt

P19537 - ALF_CORGL

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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531Glyceraldehyde 3-phosphateBy similarity
Active sitei95 – 951Proton donorBy similarity
Metal bindingi96 – 961Zinc 1; catalyticBy similarity
Metal bindingi131 – 1311Zinc 2By similarity
Metal bindingi161 – 1611Zinc 2By similarity
Metal bindingi212 – 2121Zinc 1; catalyticBy similarity
Binding sitei213 – 2131Dihydroxyacetone phosphate; via amide nitrogenBy similarity
Metal bindingi252 – 2521Zinc 1; catalyticBy similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
Synonyms:fda
Ordered Locus Names:Cgl2770, cg3068
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 344343Fructose-bisphosphate aldolasePRO_0000178712Add
BLAST

2D gel databases

World-2DPAGE0001:P19537.

Interactioni

Protein-protein interaction databases

STRINGi196627.cg3068.

Structurei

3D structure databases

ProteinModelPortaliP19537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni253 – 2553Dihydroxyacetone phosphate bindingBy similarity
Regioni274 – 2774Dihydroxyacetone phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0191.
HOGENOMiHOG000227794.
KOiK01624.
OMAiVDNEIND.
OrthoDBiEOG69GZPB.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19537-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPIATPEVYN EMLDRAKEGG FAFPAINCTS SETINAALKG FAEAESDGII
60 70 80 90 100
QFSTGGAEFG SGLAVKNKVK GAVALAAFAH EAAKSYGINV ALHTDHCQKE
110 120 130 140 150
VLDEYVRPLL AISQERVDRG ELPLFQSHMW DGSAVPIDEN LEIAQELLAK
160 170 180 190 200
AKAANIILEV EIGVVGGEED GVEAKAGANL YTSPEDFEKT IDAIGTGEKG
210 220 230 240 250
RYLLAATFGN VHGVYKPGNV KLRPEVLLEG QQVARKKLGL ADDALPFDFV
260 270 280 290 300
FHGGSGSEKE KIEEALTYGV IKMNVDTDTQ YAFTRPIVSH MFENYNGVLK
310 320 330 340
IDGEVGNKKA YDPRSYMKKA EQSMSERIIE SCQDLKSVGK TTSK
Length:344
Mass (Da):37,214
Last modified:January 23, 2007 - v3
Checksum:i745B189A2AC72BBF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti280 – 2801Q → H in CAA35190. (PubMed:2615658)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17313 Genomic DNA. Translation: CAA35190.1.
BA000036 Genomic DNA. Translation: BAC00164.1.
BX927156 Genomic DNA. Translation: CAF20791.1.
PIRiS09283.
RefSeqiNP_601964.1. NC_003450.3.
YP_227007.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAC00164; BAC00164; BAC00164.
CAF20791; CAF20791; cg3068.
GeneIDi1020714.
3345516.
KEGGicgl:NCgl2673.
PATRICi21497578. VBICorGlu203724_2701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17313 Genomic DNA. Translation: CAA35190.1 .
BA000036 Genomic DNA. Translation: BAC00164.1 .
BX927156 Genomic DNA. Translation: CAF20791.1 .
PIRi S09283.
RefSeqi NP_601964.1. NC_003450.3.
YP_227007.1. NC_006958.1.

3D structure databases

ProteinModelPortali P19537.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg3068.

2D gel databases

World-2DPAGE 0001:P19537.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC00164 ; BAC00164 ; BAC00164 .
CAF20791 ; CAF20791 ; cg3068 .
GeneIDi 1020714.
3345516.
KEGGi cgl:NCgl2673.
PATRICi 21497578. VBICorGlu203724_2701.

Phylogenomic databases

eggNOGi COG0191.
HOGENOMi HOG000227794.
KOi K01624.
OMAi VDNEIND.
OrthoDBi EOG69GZPB.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00183 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view ]
Pfami PF01116. F_bP_aldolase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsi TIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, nucleotide sequence and fine-structural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-1,6-biphosphate aldolase to class I and class II aldolases."
    von der Osten C.H., Barbas C.F. III, Wong C.-H., Sinskey A.J.
    Mol. Microbiol. 3:1625-1637(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
    Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiALF_CORGL
AccessioniPrimary (citable) accession number: P19537
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3