Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P19537

- ALF_CORGL

UniProt

P19537 - ALF_CORGL

Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531Glyceraldehyde 3-phosphateBy similarity
    Active sitei95 – 951Proton donorBy similarity
    Metal bindingi96 – 961Zinc 1; catalyticBy similarity
    Metal bindingi131 – 1311Zinc 2By similarity
    Metal bindingi161 – 1611Zinc 2By similarity
    Metal bindingi212 – 2121Zinc 1; catalyticBy similarity
    Binding sitei213 – 2131Dihydroxyacetone phosphate; via amide nitrogenBy similarity
    Metal bindingi252 – 2521Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase (EC:4.1.2.13)
    Short name:
    FBP aldolase
    Short name:
    FBPA
    Alternative name(s):
    Fructose-1,6-bisphosphate aldolase
    Gene namesi
    Name:fba
    Synonyms:fda
    Ordered Locus Names:Cgl2770, cg3068
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 344343Fructose-bisphosphate aldolasePRO_0000178712Add
    BLAST

    2D gel databases

    World-2DPAGE0001:P19537.

    Interactioni

    Protein-protein interaction databases

    STRINGi196627.cg3068.

    Structurei

    3D structure databases

    ProteinModelPortaliP19537.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni253 – 2553Dihydroxyacetone phosphate bindingBy similarity
    Regioni274 – 2774Dihydroxyacetone phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0191.
    HOGENOMiHOG000227794.
    KOiK01624.
    OMAiVDNEIND.
    OrthoDBiEOG69GZPB.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view]
    PfamiPF01116. F_bP_aldolase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsiTIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19537-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPIATPEVYN EMLDRAKEGG FAFPAINCTS SETINAALKG FAEAESDGII    50
    QFSTGGAEFG SGLAVKNKVK GAVALAAFAH EAAKSYGINV ALHTDHCQKE 100
    VLDEYVRPLL AISQERVDRG ELPLFQSHMW DGSAVPIDEN LEIAQELLAK 150
    AKAANIILEV EIGVVGGEED GVEAKAGANL YTSPEDFEKT IDAIGTGEKG 200
    RYLLAATFGN VHGVYKPGNV KLRPEVLLEG QQVARKKLGL ADDALPFDFV 250
    FHGGSGSEKE KIEEALTYGV IKMNVDTDTQ YAFTRPIVSH MFENYNGVLK 300
    IDGEVGNKKA YDPRSYMKKA EQSMSERIIE SCQDLKSVGK TTSK 344
    Length:344
    Mass (Da):37,214
    Last modified:January 23, 2007 - v3
    Checksum:i745B189A2AC72BBF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti280 – 2801Q → H in CAA35190. (PubMed:2615658)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17313 Genomic DNA. Translation: CAA35190.1.
    BA000036 Genomic DNA. Translation: BAC00164.1.
    BX927156 Genomic DNA. Translation: CAF20791.1.
    PIRiS09283.
    RefSeqiNP_601964.1. NC_003450.3.
    YP_227007.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAC00164; BAC00164; BAC00164.
    CAF20791; CAF20791; cg3068.
    GeneIDi1020714.
    KEGGicgb:cg3068.
    cgl:NCgl2673.
    PATRICi21497578. VBICorGlu203724_2701.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17313 Genomic DNA. Translation: CAA35190.1 .
    BA000036 Genomic DNA. Translation: BAC00164.1 .
    BX927156 Genomic DNA. Translation: CAF20791.1 .
    PIRi S09283.
    RefSeqi NP_601964.1. NC_003450.3.
    YP_227007.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali P19537.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg3068.

    2D gel databases

    World-2DPAGE 0001:P19537.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC00164 ; BAC00164 ; BAC00164 .
    CAF20791 ; CAF20791 ; cg3068 .
    GeneIDi 1020714.
    KEGGi cgb:cg3068.
    cgl:NCgl2673.
    PATRICi 21497578. VBICorGlu203724_2701.

    Phylogenomic databases

    eggNOGi COG0191.
    HOGENOMi HOG000227794.
    KOi K01624.
    OMAi VDNEIND.
    OrthoDBi EOG69GZPB.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view ]
    Pfami PF01116. F_bP_aldolase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsi TIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, nucleotide sequence and fine-structural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-1,6-biphosphate aldolase to class I and class II aldolases."
      von der Osten C.H., Barbas C.F. III, Wong C.-H., Sinskey A.J.
      Mol. Microbiol. 3:1625-1637(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
      Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
    2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiALF_CORGL
    AccessioniPrimary (citable) accession number: P19537
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3