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Protein

Neurofilament light polypeptide

Gene

Nefl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Miscellaneous

NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.

GO - Molecular functioni

GO - Biological processi

  • anterograde axonal transport Source: Ensembl
  • axonal transport of mitochondrion Source: Ensembl
  • cerebral cortex development Source: RGD
  • hippocampus development Source: RGD
  • intermediate filament polymerization or depolymerization Source: RGD
  • locomotion Source: Ensembl
  • microtubule cytoskeleton organization Source: Ensembl
  • negative regulation of neuron apoptotic process Source: Ensembl
  • neurofilament bundle assembly Source: RGD
  • neurofilament cytoskeleton organization Source: Ensembl
  • neuromuscular process controlling balance Source: Ensembl
  • neuron projection morphogenesis Source: Ensembl
  • peripheral nervous system axon regeneration Source: Ensembl
  • positive regulation of axonogenesis Source: Ensembl
  • protein polymerization Source: RGD
  • regulation of axon diameter Source: Ensembl
  • response to acrylamide Source: RGD
  • response to corticosterone Source: RGD
  • response to organic substance Source: RGD
  • response to peptide hormone Source: RGD
  • response to sodium arsenite Source: RGD
  • response to toxic substance Source: RGD
  • retrograde axonal transport Source: Ensembl
  • spinal cord development Source: RGD

Enzyme and pathway databases

ReactomeiR-RNO-438066 Unblocking of NMDA receptor, glutamate binding and activation
R-RNO-442729 CREB phosphorylation through the activation of CaMKII
R-RNO-442982 Ras activation upon Ca2+ influx through NMDA receptor
R-RNO-5673001 RAF/MAP kinase cascade

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofilament light polypeptide
Short name:
NF-L
Alternative name(s):
68 kDa neurofilament protein
Neurofilament triplet L protein
Gene namesi
Name:Nefl
Synonyms:Nf68, Nfl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi621458 Nefl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000637902 – 542Neurofilament light polypeptideAdd BLAST541

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine2 Publications1
GlycosylationiCAR_00012821O-linked (GlcNAc) threonine1 Publication1
Modified residuei23Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei23Omega-N-methylarginine; alternateBy similarity1
GlycosylationiCAR_00012927O-linked (GlcNAc) serine1 Publication1
Modified residuei30Omega-N-methylarginineBy similarity1
Modified residuei43PhosphotyrosineBy similarity1
Modified residuei56PhosphoserineBy similarity1
Modified residuei67PhosphoserineCombined sources1
Modified residuei103PhosphoserineCombined sources1
Modified residuei453PhosphoserineCombined sources1
Modified residuei473PhosphoserineCombined sources1
Modified residuei503PhosphoserineCombined sources1
Modified residuei519PhosphothreonineCombined sources1
Modified residuei522PhosphoserineCombined sources1
Modified residuei531PhosphoserineCombined sources1

Post-translational modificationi

O-glycosylated; contains three N-acetylglucosamine side chains.1 Publication
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.By similarity
Ubiquitinated in the presence of TRIM2 and UBE2D1.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP19527
PRIDEiP19527

2D gel databases

World-2DPAGEi0004:P19527

PTM databases

GlyConnecti431
iPTMnetiP19527
PhosphoSitePlusiP19527
SwissPalmiP19527
UniCarbKBiP19527

Expressioni

Gene expression databases

BgeeiENSRNOG00000013658
GenevisibleiP19527 RN

Interactioni

Subunit structurei

Interacts with ARHGEF28. Interacts with TRIM2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249778, 5 interactors
DIPiDIP-1N
IntActiP19527, 1 interactor
STRINGi10116.ENSRNOP00000018599

Structurei

3D structure databases

ProteinModelPortaliP19527
SMRiP19527
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 401IF rodPROSITE-ProRule annotationAdd BLAST312

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 93HeadAdd BLAST92
Regioni94 – 125Coil 1AAdd BLAST32
Regioni126 – 138Linker 1Add BLAST13
Regioni139 – 234Coil 1BAdd BLAST96
Regioni235 – 253Linker 12Add BLAST19
Regioni254 – 272Coil 2AAdd BLAST19
Regioni273 – 281Linker 29
Regioni282 – 397Coil 2BAdd BLAST116
Regioni382 – 392Epitope; recognized by IF-specific monoclonal antibodyAdd BLAST11
Regioni398 – 542TailAdd BLAST145
Regioni398 – 444Tail, subdomain AAdd BLAST47
Regioni445 – 542Tail, subdomain B (acidic)Add BLAST98

Domaini

The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGME Eukaryota
ENOG410XPTM LUCA
GeneTreeiENSGT00910000143989
HOGENOMiHOG000230977
HOVERGENiHBG013015
InParanoidiP19527
KOiK04572
OMAiEMDVSSK
OrthoDBiEOG091G12MK
PhylomeDBiP19527
TreeFamiTF330122

Family and domain databases

InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR006821 Intermed_filament_DNA-bd
IPR027692 NF-L
PANTHERiPTHR23239 PTHR23239, 1 hit
PTHR23239:SF22 PTHR23239:SF22, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF04732 Filament_head, 1 hit
SMARTiView protein in SMART
SM01391 Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFSYEPYF STSYKRRYVE TPRVHISSVR SGYSTARSAY SSYSAPVSSS
60 70 80 90 100
LSVRRSYSSS SGSLMPSLEN LDLSQVAAIS NDLKSIRTQE KAQLQDLNDR
110 120 130 140 150
FASFIERVHE LEQQNKVLEA ELLVLRQKHS EPSRFRALYE QEIRDLRLAA
160 170 180 190 200
EDATNEKQAL QGEREGLEET LRNLQARYEE EVLSREDAEG RLMEARKGAD
210 220 230 240 250
EAALARAELE KRIDSLMDEI AFLKKVHEEE IAELQAQIQY AQISVEMDVS
260 270 280 290 300
SKPDLSAALK DIRAQYEKLA AKNMQNAEEW FKSRFTVLTE SAAKNTDAVR
310 320 330 340 350
AAKDEVSESR RLLKAKTLEI EACRGMNEAL EKQLQELEDK QNADISAMQD
360 370 380 390 400
TINKLENELR STKSEMARYL KEYQDLLNVK MALDIEIAAY RKLLEGEETR
410 420 430 440 450
LSFTSVGSIT SGYSQSSQVF GRSAYSGLQS SSYLMSARAF PAYYTSHVQE
460 470 480 490 500
EQSEVEETIE ATKAEEAKDE PPSEGEAEEE EKEKEEGEEE EGAEEEEAAK
510 520 530 540
DESEDAKEEE GGEGEEEDTK ESEEEEKKEE SAGEEQAAKK KD
Length:542
Mass (Da):61,335
Last modified:January 23, 2007 - v3
Checksum:i1FCC739AF23BCCA8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198 – 203GADEAA → KARMSS in AAA41694 (PubMed:3925999).Curated6
Sequence conflicti400R → K in AAA41694 (PubMed:3925999).Curated1
Sequence conflicti477A → E in AAA41694 (PubMed:3925999).Curated1
Sequence conflicti481 – 484EKEK → KKDE in AAA41694 (PubMed:3925999).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031880 mRNA Translation: AAB87069.1
M25638 mRNA Translation: AAA41694.1
X53981 Genomic DNA Translation: CAA37931.1
PIRiA21762
I60434
RefSeqiNP_113971.1, NM_031783.1
UniGeneiRn.18568

Genome annotation databases

EnsembliENSRNOT00000018599; ENSRNOP00000018599; ENSRNOG00000013658
GeneIDi83613
KEGGirno:83613
UCSCiRGD:621458 rat

Entry informationi

Entry nameiNFL_RAT
AccessioniPrimary (citable) accession number: P19527
Secondary accession number(s): Q63367
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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