Reviewed, UniProtKB/Swiss-Prot P19514 (IPYR_BACP3)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:

ppa

Organism

Bacillus PS3 (Thermophilic bacterium PS-3)

Taxonomic identifier

2334 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subunit structure	Homotrimer. In presence of divalent cations the trimers aggregate to form a hexamer. HAMAP MF_00209
Subcellular location	Cytoplasm. HAMAP MF_00209
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 165	164	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137478
Sites
	Metal binding	57	1	Magnesium 1 By similarity
	Metal binding	62	1	Magnesium 1 By similarity
	Metal binding	62	1	Magnesium 2 By similarity
	Metal binding	94	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19514-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 38BD173CE5622C2F	FASTA	165	18,923
	10 20 30 40 50 60 MAFENKIVEA FIEIPTGSQN KYEFDKERGI FKLDRVLYSP MFYPAEYGYL QNTLALDGDP 70 80 90 100 110 120 LDILVITTNP PFPGCVIDTR VIGYLNMVDS GEEDAKLIGV PVEDPRFDEV RSIEDLPQHK 130 140 150 160 LKEIAHFFER YKDLQGKRTE IGTWEGPEAA AKLIDECIAR YNEQK

References

[1]	"Primary structure of the inorganic pyrophosphatase from thermophilic bacterium PS-3." Ichiba T., Takenaka O., Samejima T., Hachimori A. J. Biochem. 108:572-578(1990) [PubMed: 1963432] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-165.

Cross-references

Sequence databases
PIR	JX0135.
3D structure databases
HSSP	HSSP built from PDB template 1FAJ based on UniProtKB P17288.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.6.1.1. 276726.
Family and domain databases
HAMAP	MF_00209. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
ProDom	PD002014. Inorg_pphsph. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_BACP3

Accession

Primary (citable) accession number: P19514

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)