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Protein

Protein Vpx

Gene

vpx

Organism
Simian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm) (Simian immunodeficiency virus sooty mangabey monkey)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi39 – 391Zinc; via pros nitrogen1 Publication
Metal bindingi82 – 821Zinc; via tele nitrogen1 Publication
Metal bindingi87 – 871Zinc1 Publication
Metal bindingi89 – 891Zinc1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Viral immunoevasion

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Vpx
Alternative name(s):
Viral protein X
X ORF protein
Gene namesi
Name:vpx
OrganismiSimian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm) (Simian immunodeficiency virus sooty mangabey monkey)
Taxonomic identifieri11738 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusPrimate lentivirus group
Virus hostiCercopithecidae (Old World monkeys) [TaxID: 9527]
Proteomesi
  • UP000007221 Componenti: Genome

Subcellular locationi

  • Virion
  • Host nucleus

  • Note: Nuclear just after virion uncoating, or if expressed in the absence of unprocessed GAG.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 112112Protein VpxPRO_0000085407Add
BLAST

Interactioni

Subunit structurei

Interacts with the P6 region of unprocessed GAG. Interacts with host VPRBP/DCAF1, leading to change substrate specificity of the CUL4A-DDB1 E3 ligase complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VPRBPQ9Y4B62EBI-6558117,EBI-1996353From a different organism.

Protein-protein interaction databases

DIPiDIP-60689N.
IntActiP19508. 1 interaction.

Structurei

Secondary structure

1
112
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 153Combined sources
Helixi18 – 3619Combined sources
Turni37 – 393Combined sources
Helixi42 – 5615Combined sources
Turni57 – 604Combined sources
Helixi64 – 8421Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CC9X-ray2.47B1-112[»]
ProteinModelPortaliP19508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi65 – 728Nuclear localization signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi101 – 1099Poly-Pro

Sequence similaritiesi

Belongs to the lentivirus VPX protein family.Curated

Family and domain databases

InterProiIPR000012. RetroV_VpR/X.
[Graphical view]
PfamiPF00522. VPR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDPRERIPP GNSGEETIGE AFDWLDRTVE EINRAAVNHL PRELIFQVWR
60 70 80 90 100
RSWEYWHDEM GMSVSYTKYR YLCLIQKAMF MHCKKGCRCL GGEHGAGGWR
110
PGPPPPPPPG LA
Length:112
Mass (Da):12,855
Last modified:February 1, 1991 - v1
Checksum:i226D0BF3A0FD2713
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03298 Genomic RNA. Translation: AAA47779.1.
M31325 Genomic RNA. Translation: AAA47755.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03298 Genomic RNA. Translation: AAA47779.1.
M31325 Genomic RNA. Translation: AAA47755.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CC9X-ray2.47B1-112[»]
ProteinModelPortaliP19508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60689N.
IntActiP19508. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000012. RetroV_VpR/X.
[Graphical view]
PfamiPF00522. VPR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence analysis and acute pathogenicity of molecularly cloned SIVSMM-PBj14."
    Dewhurst S., Embretson J.E., Anderson D.C., Mullins J.I., Fultz P.N.
    Nature 345:636-640(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Molecular clones from a non-acutely pathogenic derivative of SIVsmmPBj14: characterization and comparison to acutely pathogenic clones."
    Dewhurst S., Embretson J.E., Fultz P.N., Mullins J.I.
    AIDS Res. Hum. Retroviruses 8:1179-1187(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "The human immunodeficiency virus type 2 Vpx protein usurps the CUL4A-DDB1 DCAF1 ubiquitin ligase to overcome a postentry block in macrophage infection."
    Bergamaschi A., Ayinde D., David A., Le Rouzic E., Morel M., Collin G., Descamps D., Damond F., Brun-Vezinet F., Nisole S., Margottin-Goguet F., Pancino G., Transy C.
    J. Virol. 83:4854-4860(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HUMAN VPRBP.
    Strain: Isolate GH-1.
  4. "Structural basis of lentiviral subversion of a cellular protein degradation pathway."
    Schwefel D., Groom H.C., Boucherit V.C., Christodoulou E., Walker P.A., Stoye J.P., Bishop K.N., Taylor I.A.
    Nature 505:234-238(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.47 ANGSTROMS) IN COMPLEX WITH ZINC; HUMAN SAMHD1 AND VPRBP, FUNCTION, INTERACTION WITH HUMAN SAMHD1 AND VPRBP.

Entry informationi

Entry nameiVPX_SIVSP
AccessioniPrimary (citable) accession number: P19508
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: January 7, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.