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Protein

Protein Vpx

Gene

vpx

Organism
Simian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm) (Simian immunodeficiency virus sooty mangabey monkey)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39Zinc; via pros nitrogen1 Publication1
Metal bindingi82Zinc; via tele nitrogen1 Publication1
Metal bindingi87Zinc1 Publication1
Metal bindingi89Zinc1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Viral immunoevasion

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Vpx
Alternative name(s):
Viral protein X
X ORF protein
Gene namesi
Name:vpx
OrganismiSimian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm) (Simian immunodeficiency virus sooty mangabey monkey)
Taxonomic identifieri11738 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusPrimate lentivirus group
Virus hostiCercopithecidae (Old World monkeys) [TaxID: 9527]
Proteomesi
  • UP000007221 Componenti: Genome

Subcellular locationi

  • Virion
  • Host nucleus

  • Note: Nuclear just after virion uncoating, or if expressed in the absence of unprocessed GAG.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000854071 – 112Protein VpxAdd BLAST112

Interactioni

Subunit structurei

Interacts with the P6 region of unprocessed GAG. Interacts with host VPRBP/DCAF1, leading to change substrate specificity of the CUL4A-DDB1 E3 ligase complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VPRBPQ9Y4B62EBI-6558117,EBI-1996353From a different organism.

Protein-protein interaction databases

DIPiDIP-60689N.
IntActiP19508. 1 interactor.

Structurei

Secondary structure

1112
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 15Combined sources3
Helixi18 – 36Combined sources19
Turni37 – 39Combined sources3
Helixi42 – 56Combined sources15
Turni57 – 60Combined sources4
Helixi64 – 84Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CC9X-ray2.47B1-112[»]
ProteinModelPortaliP19508.
SMRiP19508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi65 – 72Nuclear localization signalBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi101 – 109Poly-Pro9

Sequence similaritiesi

Belongs to the lentivirus VPX protein family.Curated

Family and domain databases

InterProiIPR000012. RetroV_VpR/X.
[Graphical view]
PfamiPF00522. VPR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDPRERIPP GNSGEETIGE AFDWLDRTVE EINRAAVNHL PRELIFQVWR
60 70 80 90 100
RSWEYWHDEM GMSVSYTKYR YLCLIQKAMF MHCKKGCRCL GGEHGAGGWR
110
PGPPPPPPPG LA
Length:112
Mass (Da):12,855
Last modified:February 1, 1991 - v1
Checksum:i226D0BF3A0FD2713
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03298 Genomic RNA. Translation: AAA47779.1.
M31325 Genomic RNA. Translation: AAA47755.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03298 Genomic RNA. Translation: AAA47779.1.
M31325 Genomic RNA. Translation: AAA47755.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CC9X-ray2.47B1-112[»]
ProteinModelPortaliP19508.
SMRiP19508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60689N.
IntActiP19508. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000012. RetroV_VpR/X.
[Graphical view]
PfamiPF00522. VPR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVPX_SIVSP
AccessioniPrimary (citable) accession number: P19508
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.