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P19499

- FRHB_METTH

UniProt

P19499 - FRHB_METTH

Protein

Coenzyme F420 hydrogenase subunit beta

Gene

frhB

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.

    Catalytic activityi

    H2 + oxidized coenzyme F420 = reduced coenzyme F420.

    Cofactori

    Nickel.
    Iron-sulfur. There are 12-13 Fe atoms per alpha/beta/gamma unit of the FRH.
    FAD.

    GO - Molecular functioni

    1. coenzyme F420 hydrogenase activity Source: UniProtKB-EC
    2. flavin adenine dinucleotide binding Source: InterPro
    3. iron-sulfur cluster binding Source: UniProtKB-KW
    4. nickel cation binding Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciMTHE187420:GJNM-1299-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coenzyme F420 hydrogenase subunit beta (EC:1.12.98.1)
    Alternative name(s):
    8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta
    Short name:
    FRH
    Gene namesi
    Name:frhB
    Ordered Locus Names:MTH_1297
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 281280Coenzyme F420 hydrogenase subunit betaPRO_0000159229Add
    BLAST

    Interactioni

    Subunit structurei

    Heterocomplex of the form (alpha1beta1gamma1)8.

    Protein-protein interaction databases

    IntActiP19499. 1 interaction.
    STRINGi187420.MTH1297.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FrhB family.Curated

    Phylogenomic databases

    eggNOGiCOG1035.
    KOiK00441.
    OMAiMENFPYN.

    Family and domain databases

    InterProiIPR007516. Co_F420_Hydgase/DH_bsu_N.
    IPR017679. Coenz_F420_hydrogenase_bsu.
    IPR007525. FrhB_FdhB_C.
    [Graphical view]
    PfamiPF04432. FrhB_FdhB_C. 1 hit.
    PF04422. FrhB_FdhB_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03289. frhB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19499-1 [UniParc]FASTAAdd to Basket

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    MVLGTYKEIV SARSTDREIQ KLAQDGGIVT GLLAYALDEG IIEGAVVAGP    50
    GKEFWKPEPM VAMTSDELKA AAGTKYTFSP NVLMLKKAVR QYGIEKLGTV 100
    AIPCQTMGIR KAQTYPFGVR FVADKIKLLV GIYCMENFPY TSLQTFICEK 150
    LGLNMELVEK MDIGKGKFWV YTQDDVYTLP LKETHGYEQA GCKICKDYVA 200
    ELADVSTGSV GSPDGWSTVI TRTDSGDSIL KQAVEAGIFE TKPIEEVKPG 250
    LGLLEKLSAQ KKEKAEKNIA ARKEMGLPTP Y 281
    Length:281
    Mass (Da):30,771
    Last modified:January 23, 2007 - v4
    Checksum:i350DCEE9029C09E1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41G → C(PubMed:9371463)Curated
    Sequence conflicti230 – 2301L → F in AAA72190. (PubMed:2207102)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02914 Genomic DNA. Translation: AAA72190.1.
    AE000666 Genomic DNA. Translation: AAB85777.1.
    PIRiD35620.
    RefSeqiNP_276416.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB85777; AAB85777; MTH_1297.
    GeneIDi1471014.
    KEGGimth:MTH1297.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02914 Genomic DNA. Translation: AAA72190.1 .
    AE000666 Genomic DNA. Translation: AAB85777.1 .
    PIRi D35620.
    RefSeqi NP_276416.1. NC_000916.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P19499. 1 interaction.
    STRINGi 187420.MTH1297.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB85777 ; AAB85777 ; MTH_1297 .
    GeneIDi 1471014.
    KEGGi mth:MTH1297.

    Phylogenomic databases

    eggNOGi COG1035.
    KOi K00441.
    OMAi MENFPYN.

    Enzyme and pathway databases

    BioCyci MTHE187420:GJNM-1299-MONOMER.

    Family and domain databases

    InterProi IPR007516. Co_F420_Hydgase/DH_bsu_N.
    IPR017679. Coenz_F420_hydrogenase_bsu.
    IPR007525. FrhB_FdhB_C.
    [Graphical view ]
    Pfami PF04432. FrhB_FdhB_C. 1 hit.
    PF04422. FrhB_FdhB_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR03289. frhB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H."
      Alex L.A., Reeve J.N., Orme-Johnson W.H., Walsh C.T.
      Biochemistry 29:7237-7244(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31 AND 185-201.
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
    3. "8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization."
      Fox J.A., Livingston D.J., Orme-Johnson W.H., Walsh C.T.
      Biochemistry 26:4219-4227(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-32.
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiFRHB_METTH
    AccessioniPrimary (citable) accession number: P19499
    Secondary accession number(s): O27356
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 89 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3