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P19499

- FRHB_METTH

UniProt

P19499 - FRHB_METTH

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Protein

Coenzyme F420 hydrogenase subunit beta

Gene

frhB

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.

Catalytic activityi

H2 + oxidized coenzyme F420 = reduced coenzyme F420.

Cofactori

Nickel.
Iron-sulfur. There are 12-13 Fe atoms per alpha/beta/gamma unit of the FRH.
FAD.

GO - Molecular functioni

  1. coenzyme F420 hydrogenase activity Source: UniProtKB-EC
  2. flavin adenine dinucleotide binding Source: InterPro
  3. iron-sulfur cluster binding Source: UniProtKB-KW
  4. nickel cation binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1299-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420 hydrogenase subunit beta (EC:1.12.98.1)
Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta
Short name:
FRH
Gene namesi
Name:frhB
Ordered Locus Names:MTH_1297
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 281280Coenzyme F420 hydrogenase subunit betaPRO_0000159229Add
BLAST

Interactioni

Subunit structurei

Heterocomplex of the form (alpha1beta1gamma1)8.

Protein-protein interaction databases

IntActiP19499. 1 interaction.
STRINGi187420.MTH1297.

Family & Domainsi

Sequence similaritiesi

Belongs to the FrhB family.Curated

Phylogenomic databases

eggNOGiCOG1035.
KOiK00441.
OMAiMENFPYN.

Family and domain databases

InterProiIPR007516. Co_F420_Hydgase/DH_bsu_N.
IPR017679. Coenz_F420_hydrogenase_bsu.
IPR007525. FrhB_FdhB_C.
[Graphical view]
PfamiPF04432. FrhB_FdhB_C. 1 hit.
PF04422. FrhB_FdhB_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03289. frhB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19499-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLGTYKEIV SARSTDREIQ KLAQDGGIVT GLLAYALDEG IIEGAVVAGP
60 70 80 90 100
GKEFWKPEPM VAMTSDELKA AAGTKYTFSP NVLMLKKAVR QYGIEKLGTV
110 120 130 140 150
AIPCQTMGIR KAQTYPFGVR FVADKIKLLV GIYCMENFPY TSLQTFICEK
160 170 180 190 200
LGLNMELVEK MDIGKGKFWV YTQDDVYTLP LKETHGYEQA GCKICKDYVA
210 220 230 240 250
ELADVSTGSV GSPDGWSTVI TRTDSGDSIL KQAVEAGIFE TKPIEEVKPG
260 270 280
LGLLEKLSAQ KKEKAEKNIA ARKEMGLPTP Y
Length:281
Mass (Da):30,771
Last modified:January 23, 2007 - v4
Checksum:i350DCEE9029C09E1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41G → C(PubMed:9371463)Curated
Sequence conflicti230 – 2301L → F in AAA72190. (PubMed:2207102)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02914 Genomic DNA. Translation: AAA72190.1.
AE000666 Genomic DNA. Translation: AAB85777.1.
PIRiD35620.
RefSeqiNP_276416.1. NC_000916.1.
WP_010876912.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85777; AAB85777; MTH_1297.
GeneIDi1471014.
KEGGimth:MTH1297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02914 Genomic DNA. Translation: AAA72190.1 .
AE000666 Genomic DNA. Translation: AAB85777.1 .
PIRi D35620.
RefSeqi NP_276416.1. NC_000916.1.
WP_010876912.1. NC_000916.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P19499. 1 interaction.
STRINGi 187420.MTH1297.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB85777 ; AAB85777 ; MTH_1297 .
GeneIDi 1471014.
KEGGi mth:MTH1297.

Phylogenomic databases

eggNOGi COG1035.
KOi K00441.
OMAi MENFPYN.

Enzyme and pathway databases

BioCyci MTHE187420:GJNM-1299-MONOMER.

Family and domain databases

InterProi IPR007516. Co_F420_Hydgase/DH_bsu_N.
IPR017679. Coenz_F420_hydrogenase_bsu.
IPR007525. FrhB_FdhB_C.
[Graphical view ]
Pfami PF04432. FrhB_FdhB_C. 1 hit.
PF04422. FrhB_FdhB_N. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR03289. frhB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H."
    Alex L.A., Reeve J.N., Orme-Johnson W.H., Walsh C.T.
    Biochemistry 29:7237-7244(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31 AND 185-201.
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  3. "8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization."
    Fox J.A., Livingston D.J., Orme-Johnson W.H., Walsh C.T.
    Biochemistry 26:4219-4227(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-32.
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiFRHB_METTH
AccessioniPrimary (citable) accession number: P19499
Secondary accession number(s): O27356
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 90 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3