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Protein

Coenzyme F420 hydrogenase subunit gamma

Gene

frhG

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.

Catalytic activityi

H2 + oxidized coenzyme F420 = reduced coenzyme F420.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi181 – 1811Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi184 – 1841Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi187 – 1871Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi191 – 1911Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi210 – 2101Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi213 – 2131Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi216 – 2161Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi220 – 2201Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. coenzyme F420 hydrogenase activity Source: UniProtKB-EC
  3. flavin adenine dinucleotide binding Source: InterPro
  4. nickel cation binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1300-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420 hydrogenase subunit gamma (EC:1.12.98.1)
Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
Short name:
FRH
Gene namesi
Name:frhG
Ordered Locus Names:MTH_1298
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000005223: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 236235Coenzyme F420 hydrogenase subunit gammaPRO_0000159233Add
BLAST

Interactioni

Subunit structurei

Heterocomplex of the form (alpha1beta1gamma1)8.

Protein-protein interaction databases

IntActiP19498. 1 interaction.
STRINGi187420.MTH1298.

Structurei

3D structure databases

ProteinModelPortaliP19498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 200294Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini201 – 230304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the FrhG family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1941.
KOiK00443.
OMAiCPPSPEI.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017681. Coenz_F420_hydrogenase_gsu.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
[Graphical view]
PfamiPF00037. Fer4. 2 hits.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03294. FrhG. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19498-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEENAKPRI GYIHLSGCTG DAMSLTENYD ILAELLTNMV DIVYGQTLVD
60 70 80 90 100
LWEMPEMDLA LVEGSVCLQD EHSLHELKEL REKAKLVCAF GSCAATGCFT
110 120 130 140 150
RYSRGGQQAQ PSHESFVPIA DLIDVDLAIP GCPPSPEIIA KAVVALLNND
160 170 180 190 200
MEYLQPMLDL AGYTEACGCD LQTKVVNQGL CTGCGTCAMA CQTRALDMTN
210 220 230
GRPELNSDRC IKCGICYVQC PRSWWPEEQI KKELGL
Length:236
Mass (Da):25,817
Last modified:January 23, 2007 - v4
Checksum:i47F9B17C5D98768D
GO

Sequence cautioni

The sequence AAB85778.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141H → G AA sequence (PubMed:3663585).Curated
Sequence conflicti95 – 951A → Q in AAA72189 (PubMed:2207102).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02914 Genomic DNA. Translation: AAA72189.1.
AE000666 Genomic DNA. Translation: AAB85778.1. Different initiation.
PIRiC35620.
RefSeqiNP_276417.1. NC_000916.1.

Genome annotation databases

EnsemblBacteriaiAAB85778; AAB85778; MTH_1298.
GeneIDi1471015.
KEGGimth:MTH1298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02914 Genomic DNA. Translation: AAA72189.1.
AE000666 Genomic DNA. Translation: AAB85778.1. Different initiation.
PIRiC35620.
RefSeqiNP_276417.1. NC_000916.1.

3D structure databases

ProteinModelPortaliP19498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19498. 1 interaction.
STRINGi187420.MTH1298.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB85778; AAB85778; MTH_1298.
GeneIDi1471015.
KEGGimth:MTH1298.

Phylogenomic databases

eggNOGiCOG1941.
KOiK00443.
OMAiCPPSPEI.

Enzyme and pathway databases

BioCyciMTHE187420:GJNM-1300-MONOMER.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017681. Coenz_F420_hydrogenase_gsu.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
[Graphical view]
PfamiPF00037. Fer4. 2 hits.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03294. FrhG. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H."
    Alex L.A., Reeve J.N., Orme-Johnson W.H., Walsh C.T.
    Biochemistry 29:7237-7244(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31.
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
  3. "8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization."
    Fox J.A., Livingston D.J., Orme-Johnson W.H., Walsh C.T.
    Biochemistry 26:4219-4227(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-15.
    Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Entry informationi

Entry nameiFRHG_METTH
AccessioniPrimary (citable) accession number: P19498
Secondary accession number(s): O27357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 109 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.