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P19498

- FRHG_METTH

UniProt

P19498 - FRHG_METTH

Protein

Coenzyme F420 hydrogenase subunit gamma

Gene

frhG

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.

    Catalytic activityi

    H2 + oxidized coenzyme F420 = reduced coenzyme F420.

    Cofactori

    Nickel.
    Iron-sulfur. There are 12-13 Fe atoms per alpha/beta/gamma unit of the FRH.
    FAD.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi181 – 1811Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi184 – 1841Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi187 – 1871Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi191 – 1911Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi210 – 2101Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi213 – 2131Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi216 – 2161Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi220 – 2201Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. coenzyme F420 hydrogenase activity Source: UniProtKB-EC
    3. flavin adenine dinucleotide binding Source: InterPro
    4. nickel cation binding Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciMTHE187420:GJNM-1300-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coenzyme F420 hydrogenase subunit gamma (EC:1.12.98.1)
    Alternative name(s):
    8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
    Short name:
    FRH
    Gene namesi
    Name:frhG
    Ordered Locus Names:MTH_1298
    OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri187420 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000005223: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 236235Coenzyme F420 hydrogenase subunit gammaPRO_0000159233Add
    BLAST

    Interactioni

    Subunit structurei

    Heterocomplex of the form (alpha1beta1gamma1)8.

    Protein-protein interaction databases

    IntActiP19498. 1 interaction.
    STRINGi187420.MTH1298.

    Structurei

    3D structure databases

    ProteinModelPortaliP19498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini172 – 200294Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini201 – 230304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the FrhG family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1941.
    KOiK00443.
    OMAiCPPSPEI.

    Family and domain databases

    Gene3Di3.40.50.700. 1 hit.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR017681. Coenz_F420_hydrogenase_gsu.
    IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    [Graphical view]
    PfamiPF00037. Fer4. 2 hits.
    PF01058. Oxidored_q6. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03294. FrhG. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19498-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEENAKPRI GYIHLSGCTG DAMSLTENYD ILAELLTNMV DIVYGQTLVD    50
    LWEMPEMDLA LVEGSVCLQD EHSLHELKEL REKAKLVCAF GSCAATGCFT 100
    RYSRGGQQAQ PSHESFVPIA DLIDVDLAIP GCPPSPEIIA KAVVALLNND 150
    MEYLQPMLDL AGYTEACGCD LQTKVVNQGL CTGCGTCAMA CQTRALDMTN 200
    GRPELNSDRC IKCGICYVQC PRSWWPEEQI KKELGL 236
    Length:236
    Mass (Da):25,817
    Last modified:January 23, 2007 - v4
    Checksum:i47F9B17C5D98768D
    GO

    Sequence cautioni

    The sequence AAB85778.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141H → G AA sequence (PubMed:3663585)Curated
    Sequence conflicti95 – 951A → Q in AAA72189. (PubMed:2207102)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02914 Genomic DNA. Translation: AAA72189.1.
    AE000666 Genomic DNA. Translation: AAB85778.1. Different initiation.
    PIRiC35620.
    RefSeqiNP_276417.1. NC_000916.1.

    Genome annotation databases

    EnsemblBacteriaiAAB85778; AAB85778; MTH_1298.
    GeneIDi1471015.
    KEGGimth:MTH1298.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02914 Genomic DNA. Translation: AAA72189.1 .
    AE000666 Genomic DNA. Translation: AAB85778.1 . Different initiation.
    PIRi C35620.
    RefSeqi NP_276417.1. NC_000916.1.

    3D structure databases

    ProteinModelPortali P19498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P19498. 1 interaction.
    STRINGi 187420.MTH1298.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB85778 ; AAB85778 ; MTH_1298 .
    GeneIDi 1471015.
    KEGGi mth:MTH1298.

    Phylogenomic databases

    eggNOGi COG1941.
    KOi K00443.
    OMAi CPPSPEI.

    Enzyme and pathway databases

    BioCyci MTHE187420:GJNM-1300-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.700. 1 hit.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR017681. Coenz_F420_hydrogenase_gsu.
    IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    [Graphical view ]
    Pfami PF00037. Fer4. 2 hits.
    PF01058. Oxidored_q6. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR03294. FrhG. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H."
      Alex L.A., Reeve J.N., Orme-Johnson W.H., Walsh C.T.
      Biochemistry 29:7237-7244(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31.
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
    3. "8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization."
      Fox J.A., Livingston D.J., Orme-Johnson W.H., Walsh C.T.
      Biochemistry 26:4219-4227(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-15.
      Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

    Entry informationi

    Entry nameiFRHG_METTH
    AccessioniPrimary (citable) accession number: P19498
    Secondary accession number(s): O27357
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 107 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3