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P19498 (FRHG_METTH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Coenzyme F420 hydrogenase subunit gamma

EC=1.12.98.1
Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
Short name=FRH
Gene names
Name:frhG
Ordered Locus Names:MTH_1298
OrganismMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum) [Reference proteome] [HAMAP]
Taxonomic identifier187420 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.

Catalytic activity

H2 + oxidized coenzyme F420 = reduced coenzyme F420.

Cofactor

Nickel.

Iron-sulfur. There are 12-13 Fe atoms per alpha/beta/gamma unit of the FRH.

FAD.

Subunit structure

Heterocomplex of the form (alpha1beta1gamma1)8.

Sequence similarities

Belongs to the FrhG family.

Contains 2 4Fe-4S ferredoxin-type domains.

Sequence caution

The sequence AAB85778.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1 Ref.3
Chain2 – 236235Coenzyme F420 hydrogenase subunit gamma
PRO_0000159233

Regions

Domain172 – 200294Fe-4S ferredoxin-type 1
Domain201 – 230304Fe-4S ferredoxin-type 2

Sites

Metal binding1811Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1841Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1871Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1911Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2101Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2131Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2161Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2201Iron-sulfur 1 (4Fe-4S) By similarity

Experimental info

Sequence conflict141H → G AA sequence Ref.3
Sequence conflict951A → Q in AAA72189. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P19498 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 47F9B17C5D98768D

FASTA23625,817
        10         20         30         40         50         60 
MAEENAKPRI GYIHLSGCTG DAMSLTENYD ILAELLTNMV DIVYGQTLVD LWEMPEMDLA 

        70         80         90        100        110        120 
LVEGSVCLQD EHSLHELKEL REKAKLVCAF GSCAATGCFT RYSRGGQQAQ PSHESFVPIA 

       130        140        150        160        170        180 
DLIDVDLAIP GCPPSPEIIA KAVVALLNND MEYLQPMLDL AGYTEACGCD LQTKVVNQGL 

       190        200        210        220        230 
CTGCGTCAMA CQTRALDMTN GRPELNSDRC IKCGICYVQC PRSWWPEEQI KKELGL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H."
Alex L.A., Reeve J.N., Orme-Johnson W.H., Walsh C.T.
Biochemistry 29:7237-7244(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31.
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
[2]"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics."
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. expand/collapse author list , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.
J. Bacteriol. 179:7135-7155(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.
[3]"8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization."
Fox J.A., Livingston D.J., Orme-Johnson W.H., Walsh C.T.
Biochemistry 26:4219-4227(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-15.
Strain: ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02914 Genomic DNA. Translation: AAA72189.1.
AE000666 Genomic DNA. Translation: AAB85778.1. Different initiation.
PIRC35620.
RefSeqNP_276417.1. NC_000916.1.

3D structure databases

ProteinModelPortalP19498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP19498. 1 interaction.
STRING187420.MTH1298.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB85778; AAB85778; MTH_1298.
GeneID1471015.
KEGGmth:MTH1298.

Phylogenomic databases

eggNOGCOG1941.
KOK00443.
OMACPPSPEI.
ProtClustDBCLSK876042.

Enzyme and pathway databases

BioCycMTHE187420:GJNM-1300-MONOMER.

Family and domain databases

Gene3D3.40.50.700. 1 hit.
InterProIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017681. Coenz_F420_hydrogenase_gsu.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
[Graphical view]
PfamPF00037. Fer4. 2 hits.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsTIGR03294. FrhG. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFRHG_METTH
AccessionPrimary (citable) accession number: P19498
Secondary accession number(s): O27357
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 105 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families