Coenzyme F420 hydrogenase subunit gamma - Methanobacterium thermoautotrophicum

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Reviewed, UniProtKB/Swiss-Prot P19498 (FRHG_METTH)

Last modified June 16, 2009. Version 76. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Coenzyme F420 hydrogenase subunit gamma
    EC=1.12.98.1
Alternative name(s):
    8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
      Short name=FRH

Gene names

Name:	frhG
Ordered Locus Names:	MTH_1298

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

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Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.
Catalytic activity	H₂ + coenzyme F420 = reduced coenzyme F420.
Cofactor	Nickel. Iron-sulfur. There are 12-13 Fe atoms per alpha/beta/gamma unit of the FRH. FAD.
Subunit structure	Heterocomplex of the form (alpha₁beta₁gamma₁)₈.
Sequence similarities	Belongs to the frhG family. Contains 2 4Fe-4S ferredoxin-type domains.

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Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.1 Ref.3

Chain

2 – 236

235

Coenzyme F420 hydrogenase subunit gamma

PRO_0000159233

Regions

Domain

172 – 200

4Fe-4S ferredoxin-type 1

Domain

201 – 230

4Fe-4S ferredoxin-type 2

Sites

Metal binding

181

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

184

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

187

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

191

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

210

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

213

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

216

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

220

Iron-sulfur 1 (4Fe-4S) By similarity

Experimental info

Sequence conflict

H → G AA sequence Ref.3

Sequence conflict

A → Q in AAA72189. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P19498-1 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 47F9B17C5D98768D
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FASTA

236

25,817

        10         20         30         40         50         60 
MAEENAKPRI GYIHLSGCTG DAMSLTENYD ILAELLTNMV DIVYGQTLVD LWEMPEMDLA 

        70         80         90        100        110        120 
LVEGSVCLQD EHSLHELKEL REKAKLVCAF GSCAATGCFT RYSRGGQQAQ PSHESFVPIA 

       130        140        150        160        170        180 
DLIDVDLAIP GCPPSPEIIA KAVVALLNND MEYLQPMLDL AGYTEACGCD LQTKVVNQGL 

       190        200        210        220        230 
CTGCGTCAMA CQTRALDMTN GRPELNSDRC IKCGICYVQC PRSWWPEEQI KKELGL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequence determination, and expression of the genes encoding the subunits of the nickel-containing 8-hydroxy-5-deazaflavin reducing hydrogenase from Methanobacterium thermoautotrophicum delta H." Alex L.A., Reeve J.N., Orme-Johnson W.H., Walsh C.T. Biochemistry 29:7237-7244(1990) [PubMed: 2207102] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31. Strain: Delta H.
[2]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.
[3]	"8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization." Fox J.A., Livingston D.J., Orme-Johnson W.H., Walsh C.T. Biochemistry 26:4219-4227(1987) [PubMed: 3663585] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-15. Strain: Delta H.

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Cross-references

Sequence databases
	J02914 Genomic DNA. Translation: AAA72189.1. AE000666 Genomic DNA. Translation: AAB85778.1. Different initiation.
PIR	C35620.
RefSeq	NP_276417.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1471015.
GenomeReviews	Gene locus MTH_1298 in contig AE000666_GR.
KEGG	mth:MTH1298.
NMPDR	fig\|187420.1.peg.1272.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P19498.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1298-MON.
BRENDA	1.12.98.1. 270.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR017681. Coenz_F420_hydrogenase_gsu. IPR006137. NADH_UbQ_OxRdtase-like_20kDa. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR03294. FrhG. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

FRHG_METTH

Accession

Primary (citable) accession number: P19498
Secondary accession number(s): O27357

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 76 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents