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Protein

Coenzyme F420 hydrogenase subunit gamma

Gene

frhG

Organism
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.

Catalytic activityi

H2 + oxidized coenzyme F420 = reduced coenzyme F420.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi181Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi184Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi187Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi191Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi210Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi213Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi216Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi220Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BRENDAi1.12.98.1 3256

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420 hydrogenase subunit gamma (EC:1.12.98.1)
Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
Short name:
FRH
Gene namesi
Name:frhG
Ordered Locus Names:MTH_1298
OrganismiMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri187420 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000005223 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001592332 – 236Coenzyme F420 hydrogenase subunit gammaAdd BLAST235

Interactioni

Subunit structurei

Heterocomplex of the form (alpha1beta1gamma1)8.

Protein-protein interaction databases

IntActiP19498 1 interactor.
STRINGi187420.MTH1298

Structurei

3D structure databases

ProteinModelPortaliP19498
SMRiP19498
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 2004Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST29
Domaini201 – 2304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

Belongs to the FrhG family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiarCOG02473 Archaea
COG1941 LUCA
KOiK00443
OMAiHESFVPI
OrthoDBiPOG093Z0GT7

Family and domain databases

Gene3Di3.40.50.7001 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR017681 Coenz_F420_hydrogenase_gsu
IPR006137 NADH_UbQ_OxRdtase-like_20kDa
IPR037024 NiFe_Hase_small_N_sf
PfamiView protein in Pfam
PF00037 Fer4, 2 hits
PF01058 Oxidored_q6, 1 hit
TIGRFAMsiTIGR03294 FrhG, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19498-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEENAKPRI GYIHLSGCTG DAMSLTENYD ILAELLTNMV DIVYGQTLVD
60 70 80 90 100
LWEMPEMDLA LVEGSVCLQD EHSLHELKEL REKAKLVCAF GSCAATGCFT
110 120 130 140 150
RYSRGGQQAQ PSHESFVPIA DLIDVDLAIP GCPPSPEIIA KAVVALLNND
160 170 180 190 200
MEYLQPMLDL AGYTEACGCD LQTKVVNQGL CTGCGTCAMA CQTRALDMTN
210 220 230
GRPELNSDRC IKCGICYVQC PRSWWPEEQI KKELGL
Length:236
Mass (Da):25,817
Last modified:January 23, 2007 - v4
Checksum:i47F9B17C5D98768D
GO

Sequence cautioni

The sequence AAB85778 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14H → G AA sequence (PubMed:3663585).Curated1
Sequence conflicti95A → Q in AAA72189 (PubMed:2207102).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02914 Genomic DNA Translation: AAA72189.1
AE000666 Genomic DNA Translation: AAB85778.1 Different initiation.
PIRiC35620

Genome annotation databases

EnsemblBacteriaiAAB85778; AAB85778; MTH_1298
KEGGimth:MTH_1298
PATRICifig|187420.15.peg.1269

Similar proteinsi

Entry informationi

Entry nameiFRHG_METTH
AccessioniPrimary (citable) accession number: P19498
Secondary accession number(s): O27357
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 134 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome