Reviewed,
UniProtKB/Swiss-Prot P19492 (GRIA3_RAT)
Last modified
June 16, 2009.
Version 99.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glutamate receptor 3 Short name=GluR-3 Short name=GluR-C Alternative name(s): GluR-K3 Glutamate receptor ionotropic, AMPA 3 AMPA-selective glutamate receptor 3 | ||||
| Gene names |
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| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 888 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. |
| Subunit structure | Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with PRKCABP, GRIP1 and GRIP2. Ref.5 Ref.6 Ref.7 |
| Subcellular location | Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. |
| Post-translational modification | Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-615 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-841 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis By similarity. |
| Miscellaneous | The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate. |
| Sequence similarities | Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification] |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| NSF | P18708 | 1 | EBI-77764,EBI-925742 | From a different organism. |
| Pick1 | Q9EP80 | 4 | EBI-77764,EBI-77728 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Flop (identifier: P19492-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Flip (identifier: P19492-2) The sequence of this isoform differs from the canonical sequence as follows: 770-771: NA → TP 780-780: N → S 784-784: L → I 801-805: SGGGD → AKDSG |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 22 | 22 | Potential | ||||||
| Chain | 23 – 888 | 866 | Glutamate receptor 3 | PRO_0000011537 | |||||
Regions | |||||||||
| Topological domain | 23 – 546 | 524 | Extracellular Potential | ||||||
| Transmembrane | 547 – 567 | 21 | Potential | ||||||
| Topological domain | 568 – 629 | 62 | Cytoplasmic Potential | ||||||
| Transmembrane | 630 – 650 | 21 | Potential | ||||||
| Topological domain | 651 – 817 | 167 | Extracellular Potential | ||||||
| Transmembrane | 818 – 838 | 21 | Potential | ||||||
| Topological domain | 839 – 888 | 50 | Cytoplasmic Potential | ||||||
| Region | 502 – 504 | 3 | Glutamate binding By similarity | ||||||
| Region | 680 – 681 | 2 | Glutamate binding By similarity | ||||||
Sites | |||||||||
| Binding site | 509 | 1 | Glutamate By similarity | ||||||
| Binding site | 731 | 1 | Glutamate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 871 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 881 | 1 | Phosphotyrosine By similarity | ||||||
| Lipidation | 615 | 1 | S-palmitoyl cysteine By similarity | ||||||
| Lipidation | 841 | 1 | S-palmitoyl cysteine By similarity | ||||||
| Glycosylation | 57 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 260 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 374 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 409 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 416 | 1 | N-linked (GlcNAc...) Potential | ||||||
Natural variations | |||||||||
| Alternative sequence | 770 – 771 | 2 | NA → TP in isoform Flip. | VSP_000119 | |||||
| Alternative sequence | 780 | 1 | N → S in isoform Flip. | VSP_000120 | |||||
| Alternative sequence | 784 | 1 | L → I in isoform Flip. | VSP_000121 | |||||
| Alternative sequence | 801 – 805 | 5 | SGGGD → AKDSG in isoform Flip. | VSP_000122 | |||||
Experimental info | |||||||||
| Sequence conflict | 517 – 518 | 2 | KP → NA Ref.2 | ||||||
Sequences
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References
| [1] | "A family of AMPA-selective glutamate receptors." Keinaenen K., Wisden W., Sommer B., Werner P., Herb A., Verdoorn T.A., Sakmann B., Seeburg P.H. Science 249:556-560(1990) [PubMed: 2166337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. |
| [2] | "Molecular cloning and functional expression of glutamate receptor subunit genes." Boulter J., Hollmann M., O'Shea-Greenfield A., Hartley M., Deneris E.S., Maron C., Heinemann S.F. Science 249:1033-1037(1990) [PubMed: 2168579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Forebrain. |
| [3] | "A family of glutamate receptor genes: evidence for the formation of heteromultimeric receptors with distinct channel properties." Nakanishi N., Schneider N.A., Axel R. Neuron 5:569-581(1990) [PubMed: 1699567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (VARIANT FLIP). Tissue: Brain cortex and Hippocampus. |
| [4] | "Flip and flop: a cell-specific functional switch in glutamate-operated channels of the CNS." Sommer B., Keinaenen K., Verdoorn T.A., Wisden W., Burnashev N., Herb A., Koehler M., Takagi T., Sakmann B., Seeburg P.H. Science 249:1580-1585(1990) [PubMed: 1699275] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS FLIP AND FLOP). Tissue: Brain. |
| [5] | "Clustering of AMPA receptors by the synaptic PDZ domain-containing protein PICK1." Xia J., Zhang X., Staudinger J., Huganir R.L. Neuron 22:179-187(1999) [PubMed: 10027300] [Abstract] Cited for: INTERACTION WITH PRKCABP. |
| [6] | "GRIP: a synaptic PDZ domain-containing protein that interacts with AMPA receptors." Dong H., O'Brien R.J., Fung E.T., Lanahan A.A., Worley P.F., Huganir R.L. Nature 386:279-284(1997) [PubMed: 9069286] [Abstract] Cited for: INTERACTION WITH GRIP1. Tissue: Hippocampus. |
| [7] | "Association of AMPA receptors with a subset of glutamate receptor-interacting protein in vivo." Wyszynski M., Valtschanoff J.G., Naisbitt S., Dunah A.W., Kim E., Standaert D.G., Weinberg R., Sheng M. J. Neurosci. 19:6528-6537(1999) [PubMed: 10414981] [Abstract] Cited for: INTERACTION WITH GRIP2. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M36420 mRNA. Translation: AAA41245.1. X54656 mRNA. Translation: CAA38466.1. M38062 mRNA. Translation: AAA63480.1. M85036 mRNA. Translation: AAA41241.2. | |||||||||||||||||||
| IPI | IPI00195443. IPI00231095. | ||||||||||||||||||
| PIR | C40170. | ||||||||||||||||||
| RefSeq | NP_001106213.1. NP_116785.2. | ||||||||||||||||||
| UniGene | Rn.74049 | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| |||||||||||||||||||
| SMR | P19492. Positions 416-530, 657-800. | ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| IntAct | P19492. 4 interactions. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | P19492. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENSRNOG00000007682. Rattus norvegicus. [Contig view] | ||||||||||||||||||
| GeneID | 29628. | ||||||||||||||||||
| KEGG | rno:29628. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| RGD | 70958. Gria3. | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| HOVERGEN | P19492. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | P19492. | ||||||||||||||||||
| GermOnline | ENSRNOG00000007682. Rattus norvegicus. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR001828. ANF_lig_bd_rcpt. IPR019594. Glu_rcpt_Glu/Gly-bd. IPR015683. Glutamate_receptor-rel. IPR001320. Iontro_glu_rcpt. IPR001508. NMDA_rcpt. [Graphical view] | ||||||||||||||||||
| PANTHER | PTHR18966. Glut_Rec_Related. 1 hit. | ||||||||||||||||||
| Pfam | PF01094. ANF_receptor. 1 hit. PF00060. Lig_chan. 1 hit. PF10613. Lig_chan-Glu_bd. 1 hit. [Graphical view] | ||||||||||||||||||
| PRINTS | PR00177. NMDARECEPTOR. | ||||||||||||||||||
| SMART | SM00079. PBPe. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| NextBio | 609848. | ||||||||||||||||||
Entry information
| Entry name | GRIA3_RAT | ||||||||
| Accession | Primary (citable) accession number: P19492 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


