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Reviewed, UniProtKB/Swiss-Prot P19492 (GRIA3_RAT)

Last modified June 16, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glutamate receptor 3
      Short name=GluR-3
      Short name=GluR-C
Alternative name(s):
    GluR-K3
    Glutamate receptor ionotropic, AMPA 3
    AMPA-selective glutamate receptor 3
Gene names
Name: Gria3
Synonyms: Glur3
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length888 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

Subunit structure

Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with PRKCABP, GRIP1 and GRIP2. Ref.5 Ref.6 Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

Post-translational modification

Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-615 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-841 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis By similarity.

Miscellaneous

The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.

Sequence similarities

Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification]

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Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
   Molecular functionIonic channel
Receptor
   PTMGlycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical term3D-structure
Gene Ontology (GO)
   Biological procession transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of receptor recycling

Inferred from mutant phenotype. Source: UniProtKB

response to lithium ion

Inferred from expression pattern. Source: UniProtKB

   Cellular componentasymmetric synapse

Inferred from direct assay. Source: RGD

cell junction

Inferred from electronic annotation. Source: UniProtKB-SubCell

dendritic shaft

Inferred from direct assay. Source: RGD

dendritic spine

Inferred from direct assay. Source: UniProtKB

ionotropic glutamate receptor complex

Traceable author statement. Source: UniProtKB

perikaryon

Inferred from direct assay. Source: RGD

postsynaptic density Ref.5

Inferred from direct assay. Source: UniProtKB

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

synaptic cleft

Inferred from direct assay. Source: RGD

terminal button

Inferred from direct assay. Source: RGD

   Molecular functionPDZ domain binding Ref.5

Inferred from physical interaction. Source: UniProtKB

alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Inferred from direct assay. Source: RGD

extracellular-glutamate-gated ion channel activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

NSFP187081EBI-77764,EBI-925742From a different organism.
Pick1Q9EP804EBI-77764,EBI-77728

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Flop (identifier: P19492-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Flip (identifier: P19492-2)

The sequence of this isoform differs from the canonical sequence as follows:
     770-771: NA → TP
     780-780: N → S
     784-784: L → I
     801-805: SGGGD → AKDSG

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 888866Glutamate receptor 3
PRO_0000011537

Regions

Topological domain23 – 546524Extracellular Potential
Transmembrane547 – 56721 Potential
Topological domain568 – 62962Cytoplasmic Potential
Transmembrane630 – 65021 Potential
Topological domain651 – 817167Extracellular Potential
Transmembrane818 – 83821 Potential
Topological domain839 – 88850Cytoplasmic Potential
Region502 – 5043Glutamate binding By similarity
Region680 – 6812Glutamate binding By similarity

Sites

Binding site5091Glutamate By similarity
Binding site7311Glutamate By similarity

Amino acid modifications

Modified residue8711Phosphotyrosine By similarity
Modified residue8811Phosphotyrosine By similarity
Lipidation6151S-palmitoyl cysteine By similarity
Lipidation8411S-palmitoyl cysteine By similarity
Glycosylation571N-linked (GlcNAc...) Potential
Glycosylation2601N-linked (GlcNAc...) Potential
Glycosylation3741N-linked (GlcNAc...) Potential
Glycosylation4091N-linked (GlcNAc...) Potential
Glycosylation4161N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence770 – 7712NA → TP in isoform Flip.
VSP_000119
Alternative sequence7801N → S in isoform Flip.
VSP_000120
Alternative sequence7841L → I in isoform Flip.
VSP_000121
Alternative sequence801 – 8055SGGGD → AKDSG in isoform Flip.
VSP_000122

Experimental info

Sequence conflict517 – 5182KP → NA Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform Flop [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 2981616375661703

FASTA888100,373
        10         20         30         40         50         60 
MGQSVLRAVF FLVLGLLGHS HGGFPNTISI GGLFMRNTVQ EHSAFRFAVQ LYNTNQNTTE 

        70         80         90        100        110        120 
KPFHLNYHVD HLDSSNSFSV TNAFCSQFSR GVYAIFGFYD QMSMNTLTSF CGALHTSFVT 

       130        140        150        160        170        180 
PSFPTDADVQ FVIQMRPALK GAILSLLSYY KWEKFVYLYD TERGFSVLQA IMEAAVQNNW 

       190        200        210        220        230        240 
QVTARSVGNI KDVQEFRRII EEMDRRQEKR YLIDCEVERI NTILEQVVIL GKHSRGYHYM 

       250        260        270        280        290        300 
LANLGFTDIL LERVMHGGAN ITGFQIVNNE NPMVQQFIQR WVRLDEREFP EAKNAPLKYT 

       310        320        330        340        350        360 
SALTHDAILV IAEAFRYLRR QRVDVSRRGS AGDCLANPAV PWSQGIDIER ALKMVQVQGM 

       370        380        390        400        410        420 
TGNIQFDTYG RRTNYTIDVY EMKVSGSRKA GYWNEYERFV PFSDQQISND SSSSENRTIV 

       430        440        450        460        470        480 
VTTILESPYV MYKKNHEQLE GNERYEGYCV DLAYEIAKHV RIKYKLSIVG DGKYGARDPE 

       490        500        510        520        530        540 
TKIWNGMVGE LVYGRADIAV APLTITLVRE EVIDFSKPFM SLGISIMIKK PQKSKPGVFS 

       550        560        570        580        590        600 
FLDPLAYEIW MCIVFAYIGV SVVLFLVSRF SPYEWHLEDN NEEPRDPQSP PDPPNEFGIF 

       610        620        630        640        650        660 
NSLWFSLGAF MQQGCDISPR SLSGRIVGGV WWFFTLIIIS SYTANLAAFL TVERMVSPIE 

       670        680        690        700        710        720 
SAEDLAKQTE IAYGTLDSGS TKEFFRRSKI AVYEKMWSYM KSAEPSVFTK TTADGVARVR 

       730        740        750        760        770        780 
KSKGKFAFLL ESTMNEYIEQ RKPCDTMKVG GNLDSKGYGV ATPKGSALGN AVNLAVLKLN 

       790        800        810        820        830        840 
EQGLLDKLKN KWWYDKGECG SGGGDSKDKT SALSLSNVAG VFYILVGGLG LAMMVALIEF 

       850        860        870        880 
CYKSRAESKR MKLTKNTQNF KPAPATNTQN YATYREGYNV YGTESVKI

« Hide

Isoform Flip.

Checksum: D53A3C20BFB3F620
Show »

FASTA888100,444

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References

[1]"A family of AMPA-selective glutamate receptors."
Keinaenen K., Wisden W., Sommer B., Werner P., Herb A., Verdoorn T.A., Sakmann B., Seeburg P.H.
Science 249:556-560(1990) [PubMed: 2166337] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Molecular cloning and functional expression of glutamate receptor subunit genes."
Boulter J., Hollmann M., O'Shea-Greenfield A., Hartley M., Deneris E.S., Maron C., Heinemann S.F.
Science 249:1033-1037(1990) [PubMed: 2168579] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Forebrain.
[3]"A family of glutamate receptor genes: evidence for the formation of heteromultimeric receptors with distinct channel properties."
Nakanishi N., Schneider N.A., Axel R.
Neuron 5:569-581(1990) [PubMed: 1699567] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (VARIANT FLIP).
Tissue: Brain cortex and Hippocampus.
[4]"Flip and flop: a cell-specific functional switch in glutamate-operated channels of the CNS."
Sommer B., Keinaenen K., Verdoorn T.A., Wisden W., Burnashev N., Herb A., Koehler M., Takagi T., Sakmann B., Seeburg P.H.
Science 249:1580-1585(1990) [PubMed: 1699275] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS FLIP AND FLOP).
Tissue: Brain.
[5]"Clustering of AMPA receptors by the synaptic PDZ domain-containing protein PICK1."
Xia J., Zhang X., Staudinger J., Huganir R.L.
Neuron 22:179-187(1999) [PubMed: 10027300] [Abstract]
Cited for: INTERACTION WITH PRKCABP.
[6]"GRIP: a synaptic PDZ domain-containing protein that interacts with AMPA receptors."
Dong H., O'Brien R.J., Fung E.T., Lanahan A.A., Worley P.F., Huganir R.L.
Nature 386:279-284(1997) [PubMed: 9069286] [Abstract]
Cited for: INTERACTION WITH GRIP1.
Tissue: Hippocampus.
[7]"Association of AMPA receptors with a subset of glutamate receptor-interacting protein in vivo."
Wyszynski M., Valtschanoff J.G., Naisbitt S., Dunah A.W., Kim E., Standaert D.G., Weinberg R., Sheng M.
J. Neurosci. 19:6528-6537(1999) [PubMed: 10414981] [Abstract]
Cited for: INTERACTION WITH GRIP2.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M36420 mRNA. Translation: AAA41245.1.
X54656 mRNA. Translation: CAA38466.1.
M38062 mRNA. Translation: AAA63480.1.
M85036 mRNA. Translation: AAA41241.2.
IPIIPI00195443.
IPI00231095.
PIRC40170.
RefSeqNP_001106213.1.
NP_116785.2.
UniGeneRn.74049

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
3DLNX-ray1.91A407-800[»]
3DP4X-ray2.11A407-800[»]
SMRP19492. Positions 416-530, 657-800.
ModBaseSearch...

Protein-protein interaction databases

IntActP19492. 4 interactions.

PTM databases

PhosphoSiteP19492.

Genome annotation databases

EnsemblENSRNOG00000007682. Rattus norvegicus. [Contig view]
GeneID29628.
KEGGrno:29628.

Organism-specific databases

RGD70958. Gria3.

Phylogenomic databases

HOVERGENP19492.

Gene expression databases

ArrayExpressP19492.
GermOnlineENSRNOG00000007682. Rattus norvegicus.

Family and domain databases

InterProIPR001828. ANF_lig_bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR015683. Glutamate_receptor-rel.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
[Graphical view]
PANTHERPTHR18966. Glut_Rec_Related. 1 hit.
PfamPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSPR00177. NMDARECEPTOR.
SMARTSM00079. PBPe. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio609848.

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Entry information

Entry nameGRIA3_RAT
AccessionPrimary (citable) accession number: P19492
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 16, 2009
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents