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P19487

- GUNA_XANCP

UniProt

P19487 - GUNA_XANCP

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Protein

Major extracellular endoglucanase

Gene

engXCA

Organism
Xanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei182 – 1821Proton donorBy similarity
Active sitei303 – 3031NucleophileBy similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC
  2. polysaccharide binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciXCAM190485:GIXZ-3519-MONOMER.

Protein family/group databases

CAZyiCBM2. Carbohydrate-Binding Module Family 2.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Major extracellular endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:engXCA
Ordered Locus Names:XCC3521
OrganismiXanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568)
Taxonomic identifieri190485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000001010: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 484459Major extracellular endoglucanasePRO_0000007875Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi190485.XCC3521.

Structurei

3D structure databases

ProteinModelPortaliP19487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni375 – 39925Thr-Pro repeats ("hinge") (Pro-Thr box)Add
BLAST

Domaini

The C-terminal region of the protein is not crucial for activity.
The Thr/Pro-rich region (also termed "hinge") may be a potential site for proteolysis.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2730.
HOGENOMiHOG000225207.
KOiK01179.
OMAiDCAAISE.
OrthoDBiEOG6FRCSN.

Family and domain databases

Gene3Di2.60.40.290. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00553. CBM_2. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTiSM00637. CBD_II. 1 hit.
[Graphical view]
SUPFAMiSSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS51173. CBM2. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19487-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIFRTASTL ALATALALAA GPAFSYSINN SRQIVDDSGK VVQLKGVNVF
60 70 80 90 100
GFETGNHVMH GLWARNWKDM IVQMQGLGFN AVRLPFCPAT LRSDTMPASI
110 120 130 140 150
DYSRNADLQG LTSLQILDKV IAEFNARGMY VLLDHHTPDC AGISELWYTG
160 170 180 190 200
SYTEAQWLAD LRFVANRYKN VPYVLGLDLK NEPHGAATWG TGNAATDWNK
210 220 230 240 250
AAERGSAAVL AVAPKWLIAV EGITDNPVCS TNGGIFWGGN LQPLACTPLN
260 270 280 290 300
IPANRLLLAP HVYGPDVFVQ SYFNDSNFPN NMPAIWERHF GQFAGTHALL
310 320 330 340 350
LGEFGGKYGE GDARDKTWQD ALVKYLRSKG INQGFYWSWN PNSGDTGGIL
360 370 380 390 400
RDDWTSVRQD KMTLLRTLWG TAGNTTPTPT PTPTPTPTPT PTPTPTPTPG
410 420 430 440 450
TSTFSTKVIV DNSWNGGYCN RVQVTNTGTA SGTWSIAVPV TGTVNNAWNA
460 470 480
TWSQSGSTLR ASGVDFNRTL AAGATAEFGF CAAS
Length:484
Mass (Da):52,242
Last modified:July 11, 2002 - v2
Checksum:i6671AE5BF1B7602A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti410 – 48475VDNSW…FCAAS → ASPVVGSAARKLPAASRLAC HWPASSTGWRVWVIAAPSVT WKRPHAARAIERRMRVTRLR RATRLNRGPLPAPAHTTQHA PRL in AAA27612. (PubMed:2373365)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32700 Genomic DNA. Translation: AAA27612.1.
AE008922 Genomic DNA. Translation: AAM42791.1.
PIRiJH0158.
RefSeqiNP_638867.1. NC_003902.1.

Genome annotation databases

EnsemblBacteriaiAAM42791; AAM42791; XCC3521.
GeneIDi1000085.
KEGGixcc:XCC3521.
PATRICi24078160. VBIXanCam115730_3766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32700 Genomic DNA. Translation: AAA27612.1 .
AE008922 Genomic DNA. Translation: AAM42791.1 .
PIRi JH0158.
RefSeqi NP_638867.1. NC_003902.1.

3D structure databases

ProteinModelPortali P19487.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190485.XCC3521.

Protein family/group databases

CAZyi CBM2. Carbohydrate-Binding Module Family 2.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM42791 ; AAM42791 ; XCC3521 .
GeneIDi 1000085.
KEGGi xcc:XCC3521.
PATRICi 24078160. VBIXanCam115730_3766.

Phylogenomic databases

eggNOGi COG2730.
HOGENOMi HOG000225207.
KOi K01179.
OMAi DCAAISE.
OrthoDBi EOG6FRCSN.

Enzyme and pathway databases

BioCyci XCAM190485:GIXZ-3519-MONOMER.

Family and domain databases

Gene3Di 2.60.40.290. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00553. CBM_2. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view ]
SMARTi SM00637. CBD_II. 1 hit.
[Graphical view ]
SUPFAMi SSF49384. SSF49384. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEi PS51173. CBM2. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the engXCA gene encoding the major endoglucanase of Xanthomonas campestris pv. campestris."
    Gough C.L., Dow J.M., Keen J., Henrissat B., Daniels M.J.
    Gene 89:53-59(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-61.
  2. "Comparison of the genomes of two Xanthomonas pathogens with differing host specificities."
    da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P.
    , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P.
    Nature 417:459-463(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33913 / NCPPB 528 / LMG 568.

Entry informationi

Entry nameiGUNA_XANCP
AccessioniPrimary (citable) accession number: P19487
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: July 11, 2002
Last modified: October 1, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3