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Protein

Elongation factor 1-alpha

Gene

tuf

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218GTPUniRule annotation
Nucleotide bindingi91 – 955GTPUniRule annotation
Nucleotide bindingi148 – 1514GTPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alphaUniRule annotation
Short name:
EF-1-alphaUniRule annotation
Alternative name(s):
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Ordered Locus Names:Ta0444
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Elongation factor 1-alphaPRO_0000090996Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi273075.Ta0444.

Structurei

3D structure databases

ProteinModelPortaliP19486.
SMRiP19486. Positions 5-422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 223219tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 218G1By similarity
Regioni70 – 745G2By similarity
Regioni91 – 944G3By similarity
Regioni148 – 1514G4By similarity
Regioni187 – 1893G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
KOiK03231.
OMAiYNEARFE.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQKPHLNL ITIGHVDHGK STLVGRLLYE HGEIPAHIIE EYRKEAEQKG
60 70 80 90 100
KATFEFAWVM DRFKEERERG VTIDLAHRKF ETDKYYFTLI DAPGHRDFVK
110 120 130 140 150
NMITGTSQAD AAILVISARE GEGVMEQTRE HAFLARTLGV PQMVVAINKM
160 170 180 190 200
DATSPPYSEK RYNEVKADAE KLLRSIGFKD ISFVPISGYK GDNVTKPSPN
210 220 230 240 250
MPWYKGPTLL QALDAFKVPE KPINKPLRIP VEDVYSITGI GTVPVGRVET
260 270 280 290 300
GVLKPGDKVI FLPADKQGDV KSIEMHHEPL QQAEPGDNIG FNVRGIAKND
310 320 330 340 350
IKRGDVCGHL DTPPTVVKAF TAQIIVLNHP SVIAPGYKPV FHVHTAQVAC
360 370 380 390 400
RIDEIVKTLN PKDGTTLKEK PDFIKNGDVA IVKVIPDKPL VIEKVSEIPQ
410 420
LGRFAVRDMG QTVAAGQCID LEKR
Length:424
Mass (Da):47,026
Last modified:December 1, 2000 - v2
Checksum:i49A71BABDEA1EF50
GO

Sequence cautioni

The sequence CAC11586.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti120 – 1201E → D in CAA37860 (PubMed:2272495).Curated
Sequence conflicti407 – 4071R → L in CAA37860 (PubMed:2272495).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53866 Genomic DNA. Translation: CAA37860.1.
AL445064 Genomic DNA. Translation: CAC11586.1. Different initiation.
PIRiS12090.
RefSeqiWP_048161592.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC11586; CAC11586; CAC11586.
GeneIDi1456053.
KEGGitac:Ta0444.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53866 Genomic DNA. Translation: CAA37860.1.
AL445064 Genomic DNA. Translation: CAC11586.1. Different initiation.
PIRiS12090.
RefSeqiWP_048161592.1. NC_002578.1.

3D structure databases

ProteinModelPortaliP19486.
SMRiP19486. Positions 5-422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta0444.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC11586; CAC11586; CAC11586.
GeneIDi1456053.
KEGGitac:Ta0444.

Phylogenomic databases

eggNOGiarCOG01561. Archaea.
COG5256. LUCA.
HOGENOMiHOG000229291.
KOiK03231.
OMAiYNEARFE.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the gene coding for the elongation factor 1 alpha from the archaebacterium Thermoplasma acidophilum."
    Tesch A., Klink F.
    FEMS Microbiol. Lett. 59:293-297(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
  2. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiEF1A_THEAC
AccessioniPrimary (citable) accession number: P19486
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: December 1, 2000
Last modified: July 6, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.