Reviewed, UniProtKB/Swiss-Prot P19485 (CYA1_RHIME)

Names and origin

Protein names

Recommended name:
    Adenylate cyclase 1
    EC=4.6.1.1
Alternative name(s):
    ATP pyrophosphate-lyase 1
    Adenylyl cyclase 1

Gene names

Name:	cya1
Synonyms:	cyaA
Ordered Locus Names:	R00259
ORF Names:	SMc00339

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	665 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays essential roles in regulation of cellular metabolism by catalyzing the synthesis of a second messenger, cAMP.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the adenylyl cyclase class-3 family. Contains 1 guanylate cyclase domain. Contains 1 HAMP domain.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cAMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 665	665	Adenylate cyclase 1		PRO_0000195744
Regions
	Transmembrane	33 – 53	21	Potential
	Transmembrane	373 – 393	21	Potential
	Domain	394 – 444	51	HAMP
	Domain	471 – 603	133	Guanylate cyclase
Sites
	Metal binding	476	1	Magnesium By similarity
	Metal binding	520	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19485-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: E6E553FE5FF77A1B	FASTA	665	71,325
	10 20 30 40 50 60 MLQRSESGFK DIESMQDSNA DKPSRTWTSS LRSLLGLVMV AMLIVVSATL VGLDYRRARS 70 80 90 100 110 120 AAIAGAEDNM DAFVGRLVDR LGALSGDTSA LVSLVASVAN SFLVPPPERM NDKVAVLREG 130 140 150 160 170 180 IARSPHIDGV YVGYPSGAFF HVVDLDSAAW RMALDAPRGA AIAVRSMERN DQGKPFDRIL 190 200 210 220 230 240 FLDASGLQFA ERHAASNGFD PRTRPWYRAA VNGKAPVAIG PYEMATTGNL GMTISQAHRG 250 260 270 280 290 300 NPQIVIGADV VLDTITDFLS RERLTDDSVS FVLDAVGRPI IHSDSTMMRR IMASKGRDRP 310 320 330 340 350 360 VATPQEDGLI ESIRRNPPPA GKATLVEVGN RTYLVTVAPL ESALLLSGHR VVVAAPLDEL 370 380 390 400 410 420 LAAANETLVQ GLAVSGAVVV VAVLLALVLA HLITKSLNQL TDSANRLQDL DFATPIDVSS 430 440 450 460 470 480 HVAEISTLNG AMNRARDAIF TFALYVPKEL VRKGIESGHF GGRAAWRQEV TAMFTDIYDF 490 500 510 520 530 540 TTISEGRSPE EVVAMLSEYF DLFSEVVAAH DGTIIQFHGD SVFAMWNAPV ADTRHAEHAC 550 560 570 580 590 600 RCALAVEERL EAFNSAQRAS GLPEFRTRFG IHTGTAVVGS VGAKERLQYT AMGDTVNVAS 610 620 630 640 650 660 RLEGMNKDYG TSVLASGAVV AQCKDMVKFR PLGTAKAKGR STALDIYEVV GVVRAVNTTE AGTAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[2]	"Rhizobium meliloti adenylate cyclase is related to eucaryotic adenylate and guanylate cyclases." Beuve A., Boesten B., Crasnier M., Danchin A., O'Gara F. J. Bacteriol. 172:2614-2621(1990) [PubMed: 1970565] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 473-665.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591688 Genomic DNA. Translation: CAC41696.1. M35096 Genomic DNA. Translation: AAA26247.1.
PIR	A35266. B35266.
RefSeq	NP_384365.1.
3D structure databases
SMR	P19485. Positions 196-366, 468-651.
ModBase	Search...
Genome annotation databases
GeneID	1231893.
GenomeReviews	Gene locus R00259 in contig AL591688_GR.
KEGG	sme:SMc00339.
NMPDR	fig|266834.1.peg.1553.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG653831.
OMA	QYTAMGD.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC00339-MONOMER.
BRENDA	4.6.1.1. 142.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR003660. HAMP_linker_domain. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 1 hit.
Pfam	PF00211. Guanylate_cyc. 1 hit. PF00672. HAMP. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. SM00304. HAMP. 1 hit. [Graphical view]
PROSITE	PS50125. GUANYLATE_CYCLASE_2. 1 hit. PS50885. HAMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYA1_RHIME

Accession

Primary (citable) accession number: P19485

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	May 30, 2000
Last modified:	January 19, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)