P19485 (CYA1_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate cyclase 1
EC=4.6.1.1

Alternative name(s):
ATP pyrophosphate-lyase 1
Adenylyl cyclase 1

Gene names

Name:	cya1
Synonyms:	cyaA
Ordered Locus Names:	R00259
ORF Names:	SMc00339

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Rhizobium meliloti

Protein attributes

Sequence length	665 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays essential roles in regulation of cellular metabolism by catalyzing the synthesis of a second messenger, cAMP.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the adenylyl cyclase class-3 family. Contains 1 guanylate cyclase domain. Contains 1 HAMP domain.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 665	665	Adenylate cyclase 1		PRO_0000195744
Regions
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	373 – 393	21	Helical; Potential
	Domain	394 – 444	51	HAMP
	Domain	471 – 603	133	Guanylate cyclase
Sites
	Metal binding	476	1	Magnesium By similarity
	Metal binding	520	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19485 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: E6E553FE5FF77A1B	FASTA	665	71,325
	10 20 30 40 50 60 MLQRSESGFK DIESMQDSNA DKPSRTWTSS LRSLLGLVMV AMLIVVSATL VGLDYRRARS 70 80 90 100 110 120 AAIAGAEDNM DAFVGRLVDR LGALSGDTSA LVSLVASVAN SFLVPPPERM NDKVAVLREG 130 140 150 160 170 180 IARSPHIDGV YVGYPSGAFF HVVDLDSAAW RMALDAPRGA AIAVRSMERN DQGKPFDRIL 190 200 210 220 230 240 FLDASGLQFA ERHAASNGFD PRTRPWYRAA VNGKAPVAIG PYEMATTGNL GMTISQAHRG 250 260 270 280 290 300 NPQIVIGADV VLDTITDFLS RERLTDDSVS FVLDAVGRPI IHSDSTMMRR IMASKGRDRP 310 320 330 340 350 360 VATPQEDGLI ESIRRNPPPA GKATLVEVGN RTYLVTVAPL ESALLLSGHR VVVAAPLDEL 370 380 390 400 410 420 LAAANETLVQ GLAVSGAVVV VAVLLALVLA HLITKSLNQL TDSANRLQDL DFATPIDVSS 430 440 450 460 470 480 HVAEISTLNG AMNRARDAIF TFALYVPKEL VRKGIESGHF GGRAAWRQEV TAMFTDIYDF 490 500 510 520 530 540 TTISEGRSPE EVVAMLSEYF DLFSEVVAAH DGTIIQFHGD SVFAMWNAPV ADTRHAEHAC 550 560 570 580 590 600 RCALAVEERL EAFNSAQRAS GLPEFRTRFG IHTGTAVVGS VGAKERLQYT AMGDTVNVAS 610 620 630 640 650 660 RLEGMNKDYG TSVLASGAVV AQCKDMVKFR PLGTAKAKGR STALDIYEVV GVVRAVNTTE AGTAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[2]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"Rhizobium meliloti adenylate cyclase is related to eucaryotic adenylate and guanylate cyclases." Beuve A., Boesten B., Crasnier M., Danchin A., O'Gara F. J. Bacteriol. 172:2614-2621(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 473-665.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591688 Genomic DNA. Translation: CAC41696.1. M35096 Genomic DNA. Translation: AAA26247.1.
PIR	A35266. B35266.
RefSeq	NP_384365.1. NC_003047.1.
3D structure databases
ProteinModelPortal	P19485.
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SMc00339.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC41696; CAC41696; SMc00339.
GeneID	1231893.
KEGG	sme:SMc00339.
PATRIC	23629713. VBISinMel96828_1626.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0840.
HOGENOM	HOG000295746.
KO	K01768.
OMA	TIASAME.
ProtClustDB	CLSK805947.
Enzyme and pathway databases
BioCyc	SMEL266834:GJF6-270-MONOMER.
Family and domain databases
Gene3D	3.30.70.1230. 1 hit.
InterPro	IPR001054. A/G_cyclase. IPR003660. HAMP_linker_domain. [Graphical view]
Pfam	PF00211. Guanylate_cyc. 1 hit. PF00672. HAMP. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. SM00304. HAMP. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 1 hit.
PROSITE	PS50125. GUANYLATE_CYCLASE_2. 1 hit. PS50885. HAMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYA1_RHIME

Accession

Primary (citable) accession number: P19485

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program