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P19480 (AHPF_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alkyl hydroperoxide reductase subunit F
EC=1.8.1.-
Alternative name(s):
Alkyl hydroperoxide reductase F52A protein
Gene names
Name:ahpF
Ordered Locus Names:STM0609
OrganismSalmonella typhimurium
Taxonomic identifier90371 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein.

Cofactor

Binds 1 FAD per subunit By similarity.

Subunit structure

Homodimer.

Miscellaneous

The active site is a redox-active disulfide bond.

Sequence similarities

Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Contains 1 glutaredoxin domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 521521Alkyl hydroperoxide reductase subunit F
PRO_0000166777

Regions

Domain109 – 208100Glutaredoxin
Nucleotide binding214 – 22916FAD By similarity
Nucleotide binding357 – 37115NAD or NADP By similarity
Nucleotide binding478 – 48811FAD By similarity

Amino acid modifications

Disulfide bond345 ↔ 348Redox-active By similarity

Experimental info

Sequence conflict91Q → N AA sequence Ref.3
Sequence conflict151E → DR AA sequence Ref.3

Secondary structure

1 ............................................................................................ 521
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P19480 [UniParc].

Last modified February 1, 1991. Version 1.
Checksum: 813DEA1398DAE26F

FASTA52155,950
        10         20         30         40         50         60 
MLDTNMKTQL RAYLEKLTKP VELIATLDDS AKSAEIKELL AEIAELSDKV TFKEDNTLPV 

        70         80         90        100        110        120 
RKPSFLITNP GSQQGPRFAG SPLGHEFTSL VLALLWTGGH PSKEAQSLLE QIRDIDGDFE 

       130        140        150        160        170        180 
FETYYSLSCH NCPDVVQALN LMAVLNPRIK HTAIDGGTFQ NEITERNVMG VPAVFVNGKE 

       190        200        210        220        230        240 
FGQGRMTLTE IVAKVDTGAE KRAAEALNKR DAYDVLIVGS GPAGAAAAVY SARKGIRTGL 

       250        260        270        280        290        300 
MGERFGGQVL DTVDIENYIS VPKTEGQKLA GALKAHVSDY DVDVIDSQSA SKLVPAATEG 

       310        320        330        340        350        360 
GLHQIETASG AVLKARSIII ATGAKWRNMN VPGEDQYRTK GVTYCPHCDG PLFKGKRVAV 

       370        380        390        400        410        420 
IGGGNSGVEA AIDLAGIVEH VTLLEFAPEM KADQVLQDKV RSLKNVDIIL NAQTTEVKGD 

       430        440        450        460        470        480 
GSKVVGLEYR DRVSGDIHSV ALAGIFVQIG LLPNTHWLEG ALERNRMGEI IIDAKCETSV 

       490        500        510        520 
KGVFAAGDCT TVPYKQIIIA TGEGAKASLS AFDYLIRTKI A

« Hide

References

« Hide 'large scale' references
[1]"Alkyl hydroperoxide reductase from Salmonella typhimurium. Sequence and homology to thioredoxin reductase and other flavoprotein disulfide oxidoreductases."
Tartaglia L.A., Storz G., Brodsky M.H., Lai A., Ames B.N.
J. Biol. Chem. 265:10535-10540(1990) [PubMed: 2191951] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed: 11677609] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
[3]"An alkyl hydroperoxide reductase from Salmonella typhimurium involved in the defense of DNA against oxidative damage. Purification and properties."
Jacobson F.S., Morgan R.W., Christman M.F., Ames B.N.
J. Biol. Chem. 264:1488-1496(1989) [PubMed: 2643600] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-25.
Strain: oxyR1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J05478 Unassigned DNA. Translation: AAA16432.1.
AE006468 Genomic DNA. Translation: AAL19560.1.
PIRB35441.
RefSeqNP_459601.1. NC_003197.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HYUX-ray2.00A1-521[»]
1ZYNX-ray2.30A/B1-202[»]
1ZYPX-ray2.40A/B1-202[»]
ProteinModelPortalP19480.
SMRP19480. Positions 1-521.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-2906N.

Proteomic databases

PRIDEP19480.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1252129.
GenomeReviewsGene locus STM0609 in contig AE006468_GR.
KEGGstm:STM0609.
PATRIC32379563. VBISalEnt20916_0641.

Phylogenomic databases

HOGENOMHBG289531.
OMAMMAAINP.
ProtClustDBPRK15317.

Enzyme and pathway databases

BioCycSTYP99287:STM0609-MONOMER.

Family and domain databases

InterProIPR012081. Alkyl_hydroperoxide_Rdtase_suF.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR002109. Glutaredoxin.
IPR008255. Pyr_nucl-diS_OxRdtase_2_AS.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
Gene3DG3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
KOK03387.
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PIRSFPIRSF000238. AhpF. 1 hit.
PRINTSPR00368. FADPNR.
PR00469. PNDRDTASEII.
SUPFAMSSF52833. Thiordxn-like_fd. 2 hits.
TIGRFAMsTIGR03140. AhpF. 1 hit.
PROSITEPS51354. GLUTAREDOXIN_2. 1 hit.
PS00573. PYRIDINE_REDOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAHPF_SALTY
AccessionPrimary (citable) accession number: P19480
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: January 25, 2012
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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