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Protein

Malate dehydrogenase, glyoxysomal

Gene
N/A
Organism
Citrullus lanatus (Watermelon) (Citrullus vulgaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Kineticsi

  1. KM=146 µM for NADH
  2. KM=76 mM for oxaloacetate

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei77NADBy similarity1
    Binding sitei124Substrate1
    Binding sitei130Substrate1
    Binding sitei137NADBy similarity1
    Binding sitei162Substrate1
    Binding sitei196Substrate1
    Active sitei220Proton acceptor1
    Binding sitei271NADBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi51 – 57NADBy similarity7
    Nucleotide bindingi160 – 162NADBy similarity3

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase, glyoxysomal (EC:1.1.1.37)
    OrganismiCitrullus lanatus (Watermelon) (Citrullus vulgaris)
    Taxonomic identifieri3654 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCitrullus

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 36Glyoxysome2 PublicationsAdd BLAST36
    ChainiPRO_000001863837 – 356Malate dehydrogenase, glyoxysomalAdd BLAST320

    Proteomic databases

    PRIDEiP19446.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1356
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi45 – 51Combined sources7
    Helixi57 – 66Combined sources10
    Beta strandi70 – 80Combined sources11
    Helixi81 – 89Combined sources9
    Beta strandi96 – 102Combined sources7
    Helixi103 – 110Combined sources8
    Beta strandi114 – 118Combined sources5
    Helixi132 – 151Combined sources20
    Beta strandi155 – 159Combined sources5
    Helixi164 – 178Combined sources15
    Beta strandi185 – 188Combined sources4
    Helixi191 – 205Combined sources15
    Helixi209 – 211Combined sources3
    Beta strandi216 – 218Combined sources3
    Helixi222 – 224Combined sources3
    Beta strandi225 – 227Combined sources3
    Helixi229 – 231Combined sources3
    Helixi240 – 260Combined sources21
    Turni261 – 263Combined sources3
    Helixi269 – 287Combined sources19
    Beta strandi292 – 299Combined sources8
    Beta strandi302 – 315Combined sources14
    Beta strandi318 – 322Combined sources5
    Helixi330 – 354Combined sources25

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1SEVX-ray2.55A/B1-356[»]
    1SMKX-ray2.50A/B/C/D/E/F/G/H37-356[»]
    ProteinModelPortaliP19446.
    SMRiP19446.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP19446.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    SSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19446-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MQPIPDVNQR IARISAHLHP PKSQMEESSA LRRANCRAKG GAPGFKVAIL
    60 70 80 90 100
    GAAGGIGQPL AMLMKMNPLV SVLHLYDVVN APGVTADISH MDTGAVVRGF
    110 120 130 140 150
    LGQQQLEAAL TGMDLIIVPA GVPRKPGMTR DDLFKINAGI VKTLCEGIAK
    160 170 180 190 200
    CCPRAIVNLI SNPVNSTVPI AAEVFKKAGT YDPKRLLGVT MLDVVRANTF
    210 220 230 240 250
    VAEVLGLDPR DVDVPVVGGH AGVTILPLLS QVKPPSSFTQ EEISYLTDRI
    260 270 280 290 300
    QNGGTEVVEA KAGAGSATLS MAYAAVKFAD ACLRGLRGDA GVIECAFVSS
    310 320 330 340 350
    QVTELPFFAS KVRLGRNGIE EVYSLGPLNE YERIGLEKAK KELAGSIEKG

    VSFIRS
    Length:356
    Mass (Da):37,637
    Last modified:February 1, 1991 - v1
    Checksum:i4B0C8101DC11FC7F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M33148 mRNA. Translation: AAA33041.1.
    PIRiA35957. DEPUGW.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M33148 mRNA. Translation: AAA33041.1.
    PIRiA35957. DEPUGW.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1SEVX-ray2.55A/B1-356[»]
    1SMKX-ray2.50A/B/C/D/E/F/G/H37-356[»]
    ProteinModelPortaliP19446.
    SMRiP19446.
    ModBaseiSearch...
    MobiDBiSearch...

    Proteomic databases

    PRIDEiP19446.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Miscellaneous databases

    EvolutionaryTraceiP19446.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    SSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMDHG_CITLA
    AccessioniPrimary (citable) accession number: P19446
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: February 1, 1991
    Last modified: November 2, 2016
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.