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Protein

Glutamine synthetase

Gene

glnII

Organism
Streptomyces viridochromogenes
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Miscellaneous

Overexpression of Streptomyces viridochromogenes glutamine synthetase confers resistance against the antibiotic phosphinathricyl-alenyl-alanine.1 Publication
Two forms of glutamine synthetase (GSI and GSII) can be found in this bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113MagnesiumBy similarity1
Metal bindingi115MagnesiumBy similarity1
Metal bindingi174MagnesiumBy similarity1
Metal bindingi181MagnesiumBy similarity1
Binding sitei279L-glutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase1 Publication (EC:6.3.1.2By similarity)
Short name:
GS1 Publication
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase II1 Publication
Short name:
GSII1 Publication
Gene namesi
Name:glnII1 Publication
OrganismiStreptomyces viridochromogenes
Taxonomic identifieri1938 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532701 – 343Glutamine synthetaseAdd BLAST343

Interactioni

Subunit structurei

Homooctamer and homotetramer.By similarity

Protein-protein interaction databases

STRINGi591159.SSQG_02153.

Structurei

3D structure databases

ProteinModelPortaliP19432.
SMRiP19432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4107R5B. Bacteria.
COG0174. LUCA.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P19432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFKAEYIWI DGTEPTAKLR SKTKIITGQP AGLDALPIWG FDGSSTNQAE
60 70 80 90 100
GHSSDCVLKP VFTCPDPIRG GDDILVLCEV LNIDLTPHAS NTRAALAEVA
110 120 130 140 150
ERFAAQEPIF GIEQEYTFFQ DGYPLGFPKG GFPAPQGGYY CGVGADEIFG
160 170 180 190 200
RDVVEAHLDN CLKAGLAISG INAEVMPGQW EFQVGPVSPL EVSDHLWVAR
210 220 230 240 250
WLLYRTAEDF DVAATLDPKP VKGDWNGAGA HTNFSTKAMR ESYDAIITAA
260 270 280 290 300
ESLGEGSKPL DHVKNYGAGI DDRLTGLHET APWNEYSYGV SDRGASVRIP
310 320 330 340
WQVEKDGKGY IEDRRPNANV DPYVVTRLLV DTCCSALEKA GQV
Length:343
Mass (Da):37,259
Last modified:February 1, 1991 - v1
Checksum:i537C8F425E2E660F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52842 Genomic DNA. Translation: CAA37028.1.
PIRiC36724. AJSM2V.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52842 Genomic DNA. Translation: CAA37028.1.
PIRiC36724. AJSM2V.

3D structure databases

ProteinModelPortaliP19432.
SMRiP19432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi591159.SSQG_02153.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107R5B. Bacteria.
COG0174. LUCA.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA2_STRVR
AccessioniPrimary (citable) accession number: P19432
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 12, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.