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Protein

Negative elongation factor E

Gene

Nelfe

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-113418. Formation of the Early Elongation Complex.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-MMU-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative elongation factor E
Short name:
NELF-E
Alternative name(s):
RNA-binding protein RD
Gene namesi
Name:Nelfe
Synonyms:D17h6s45, Rd, Rdbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:102744. Nelfe.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Negative elongation factor EPRO_0000081803Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511PhosphoserineBy similarity
Cross-linki78 – 78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei113 – 1131PhosphoserineBy similarity
Modified residuei115 – 1151PhosphoserineCombined sources
Modified residuei131 – 1311PhosphoserineCombined sources
Modified residuei139 – 1391PhosphoserineBy similarity
Modified residuei179 – 1791PhosphoserineBy similarity
Modified residuei181 – 1811PhosphoserineBy similarity
Modified residuei185 – 1851PhosphoserineBy similarity
Modified residuei253 – 2531PhosphoserineBy similarity
Modified residuei255 – 2551PhosphoserineBy similarity
Modified residuei276 – 2761PhosphothreonineBy similarity
Modified residuei278 – 2781PhosphothreonineBy similarity
Modified residuei285 – 2851PhosphoserineBy similarity
Modified residuei357 – 3571PhosphoserineCombined sources

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP19426.
PaxDbiP19426.
PeptideAtlasiP19426.
PRIDEiP19426.

PTM databases

iPTMnetiP19426.
PhosphoSiteiP19426.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024369.
CleanExiMM_RDBP.
ExpressionAtlasiP19426. baseline and differential.
GenevisibleiP19426. MM.

Interactioni

Subunit structurei

The NELF complex is composed of NELFA, NELFB, NELFCD and NELFE.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Hsp90ab1P114992EBI-6142845,EBI-492813

Protein-protein interaction databases

IntActiP19426. 1 interaction.
STRINGi10090.ENSMUSP00000094956.

Structurei

3D structure databases

ProteinModelPortaliP19426.
SMRiP19426. Positions 261-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati184 – 18521
Repeati186 – 18722
Repeati188 – 18923
Repeati190 – 19124
Repeati192 – 19325; approximate
Repeati194 – 19526
Repeati196 – 19727
Repeati198 – 19928
Repeati200 – 20129
Repeati202 – 203210; approximate
Repeati204 – 205211
Repeati206 – 207212
Repeati208 – 209213
Repeati210 – 211214
Repeati212 – 213215
Repeati214 – 215216
Repeati216 – 217217
Repeati218 – 219218; approximate
Repeati220 – 221219; approximate
Repeati222 – 223220
Repeati224 – 225221
Repeati226 – 227222
Repeati228 – 229223
Repeati230 – 231224; approximate
Repeati232 – 233225
Repeati234 – 235226
Repeati236 – 237227
Repeati238 – 239228
Repeati240 – 241229
Repeati242 – 243230
Repeati244 – 245231
Repeati246 – 247232
Domaini266 – 33671RRMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni184 – 2476432 X 2 AA approximate tandem repeats of R-[DSE]Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili7 – 3630Sequence analysisAdd
BLAST

Domaini

The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation (By similarity).By similarity

Sequence similaritiesi

Belongs to the RRM NELF-E family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IK4K. Eukaryota.
ENOG41101UX. LUCA.
GeneTreeiENSGT00630000089917.
HOGENOMiHOG000232089.
HOVERGENiHBG052598.
InParanoidiP19426.
KOiK15182.
OMAiVYIHFPS.
PhylomeDBiP19426.
TreeFamiTF324087.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033102. NELFE.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR17250:SF0. PTHR17250:SF0. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P19426-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSGP ASQGGVKRSL
60 70 80 90 100
SEQPVVDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE
110 120 130 140 150
KGPVPTFQPF QRSMSADEDL QEPSRRPQRK SLYESFVSSS DRLRELGQDG
160 170 180 190 200
EEAEAPGAGD GPPRGFDWSY EEHGSARSSA SPPRSRSRDR SHDRSRDRDR
210 220 230 240 250
DKERDRDRDR DRDRDRDKDK DRDRDRDRDK ERDRDRDRDR DRERDREGPF
260 270 280 290 300
RRSDSFPERR APRKGNTLYV YGEDMTPTLL RGAFSPFGNI IDLSMDPPRN
310 320 330 340 350
CAFVTYEKME SADQAVAELN GTQVESVQLK VNIARKQPML DAATGKSVWG
360 370
SLAVQNSPKG CHRDKRTQIV YSDDL
Length:375
Mass (Da):42,554
Last modified:November 14, 2003 - v2
Checksum:iE7B5D51A366B7949
GO
Isoform 2 (identifier: P19426-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-231: Missing.

Show »
Length:367
Mass (Da):41,483
Checksum:i7EEE2FB0BA164C7E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181K → E in AAA39680 (PubMed:3353717).Curated

Polymorphismi

The length of the RD repeats is variable.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti228 – 2292Missing in strain: LP.RIII/Sn. 1 Publication
Natural varianti229 – 2368Missing in strain: 129/Sv. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei224 – 2318Missing in isoform 2. 2 PublicationsVSP_008960

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21332 Genomic DNA. Translation: AAA39680.1.
AK012389 mRNA. Translation: BAB28206.1.
BC034867 mRNA. Translation: AAH34867.1.
AF109906 Genomic DNA. Translation: AAC84161.1.
U83841 Genomic DNA. Translation: AAB41535.1.
U83842 Genomic DNA. Translation: AAB41536.1.
CCDSiCCDS28662.1. [P19426-2]
PIRiA40112.
RefSeqiNP_001039328.1. NM_001045863.1. [P19426-2]
NP_001039329.1. NM_001045864.1. [P19426-2]
NP_613046.2. NM_138580.2. [P19426-2]
UniGeneiMm.279907.
Mm.653.

Genome annotation databases

EnsembliENSMUST00000097343; ENSMUSP00000094956; ENSMUSG00000024369. [P19426-2]
ENSMUST00000165953; ENSMUSP00000131195; ENSMUSG00000024369. [P19426-2]
ENSMUST00000173357; ENSMUSP00000134272; ENSMUSG00000024369. [P19426-2]
GeneIDi27632.
KEGGimmu:27632.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21332 Genomic DNA. Translation: AAA39680.1.
AK012389 mRNA. Translation: BAB28206.1.
BC034867 mRNA. Translation: AAH34867.1.
AF109906 Genomic DNA. Translation: AAC84161.1.
U83841 Genomic DNA. Translation: AAB41535.1.
U83842 Genomic DNA. Translation: AAB41536.1.
CCDSiCCDS28662.1. [P19426-2]
PIRiA40112.
RefSeqiNP_001039328.1. NM_001045863.1. [P19426-2]
NP_001039329.1. NM_001045864.1. [P19426-2]
NP_613046.2. NM_138580.2. [P19426-2]
UniGeneiMm.279907.
Mm.653.

3D structure databases

ProteinModelPortaliP19426.
SMRiP19426. Positions 261-347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19426. 1 interaction.
STRINGi10090.ENSMUSP00000094956.

PTM databases

iPTMnetiP19426.
PhosphoSiteiP19426.

Proteomic databases

MaxQBiP19426.
PaxDbiP19426.
PeptideAtlasiP19426.
PRIDEiP19426.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097343; ENSMUSP00000094956; ENSMUSG00000024369. [P19426-2]
ENSMUST00000165953; ENSMUSP00000131195; ENSMUSG00000024369. [P19426-2]
ENSMUST00000173357; ENSMUSP00000134272; ENSMUSG00000024369. [P19426-2]
GeneIDi27632.
KEGGimmu:27632.

Organism-specific databases

CTDi7936.
MGIiMGI:102744. Nelfe.

Phylogenomic databases

eggNOGiENOG410IK4K. Eukaryota.
ENOG41101UX. LUCA.
GeneTreeiENSGT00630000089917.
HOGENOMiHOG000232089.
HOVERGENiHBG052598.
InParanoidiP19426.
KOiK15182.
OMAiVYIHFPS.
PhylomeDBiP19426.
TreeFamiTF324087.

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-113418. Formation of the Early Elongation Complex.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-MMU-75955. RNA Polymerase II Transcription Elongation.

Miscellaneous databases

PROiP19426.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024369.
CleanExiMM_RDBP.
ExpressionAtlasiP19426. baseline and differential.
GenevisibleiP19426. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033102. NELFE.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR17250:SF0. PTHR17250:SF0. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNELFE_MOUSE
AccessioniPrimary (citable) accession number: P19426
Secondary accession number(s): P97485, P97486, Q9CZN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 14, 2003
Last modified: September 7, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.