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Protein

Negative elongation factor E

Gene

Nelfe

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-112387. Elongation arrest and recovery.
R-MMU-113418. Formation of the Early Elongation Complex.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-MMU-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative elongation factor E
Short name:
NELF-E
Alternative name(s):
RNA-binding protein RD
Gene namesi
Name:Nelfe
Synonyms:D17h6s45, Rd, Rdbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:102744. Nelfe.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818031 – 375Negative elongation factor EAdd BLAST375

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51PhosphoserineBy similarity1
Cross-linki78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei113PhosphoserineBy similarity1
Modified residuei115PhosphoserineCombined sources1
Modified residuei131PhosphoserineCombined sources1
Modified residuei139PhosphoserineBy similarity1
Modified residuei179PhosphoserineBy similarity1
Modified residuei181PhosphoserineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei253PhosphoserineBy similarity1
Modified residuei255PhosphoserineBy similarity1
Modified residuei276PhosphothreonineBy similarity1
Modified residuei278PhosphothreonineBy similarity1
Modified residuei285PhosphoserineBy similarity1
Modified residuei357PhosphoserineCombined sources1

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP19426.
PaxDbiP19426.
PeptideAtlasiP19426.
PRIDEiP19426.

PTM databases

iPTMnetiP19426.
PhosphoSitePlusiP19426.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024369.
CleanExiMM_RDBP.
ExpressionAtlasiP19426. baseline and differential.
GenevisibleiP19426. MM.

Interactioni

Subunit structurei

The NELF complex is composed of NELFA, NELFB, NELFCD and NELFE.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Hsp90ab1P114992EBI-6142845,EBI-492813

Protein-protein interaction databases

IntActiP19426. 1 interactor.
STRINGi10090.ENSMUSP00000094956.

Structurei

3D structure databases

ProteinModelPortaliP19426.
SMRiP19426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati184 – 18512
Repeati186 – 18722
Repeati188 – 18932
Repeati190 – 19142
Repeati192 – 1935; approximate2
Repeati194 – 19562
Repeati196 – 19772
Repeati198 – 19982
Repeati200 – 20192
Repeati202 – 20310; approximate2
Repeati204 – 205112
Repeati206 – 207122
Repeati208 – 209132
Repeati210 – 211142
Repeati212 – 213152
Repeati214 – 215162
Repeati216 – 217172
Repeati218 – 21918; approximate2
Repeati220 – 22119; approximate2
Repeati222 – 223202
Repeati224 – 225212
Repeati226 – 227222
Repeati228 – 229232
Repeati230 – 23124; approximate2
Repeati232 – 233252
Repeati234 – 235262
Repeati236 – 237272
Repeati238 – 239282
Repeati240 – 241292
Repeati242 – 243302
Repeati244 – 245312
Repeati246 – 247322
Domaini266 – 336RRMPROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 24732 X 2 AA approximate tandem repeats of R-[DSE]Add BLAST64

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili7 – 36Sequence analysisAdd BLAST30

Domaini

The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation (By similarity).By similarity

Sequence similaritiesi

Belongs to the RRM NELF-E family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IK4K. Eukaryota.
ENOG41101UX. LUCA.
GeneTreeiENSGT00630000089917.
HOGENOMiHOG000232089.
HOVERGENiHBG052598.
InParanoidiP19426.
KOiK15182.
OMAiVYIHFPS.
PhylomeDBiP19426.
TreeFamiTF324087.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033102. NELFE.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR17250:SF0. PTHR17250:SF0. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P19426-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSGP ASQGGVKRSL
60 70 80 90 100
SEQPVVDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE
110 120 130 140 150
KGPVPTFQPF QRSMSADEDL QEPSRRPQRK SLYESFVSSS DRLRELGQDG
160 170 180 190 200
EEAEAPGAGD GPPRGFDWSY EEHGSARSSA SPPRSRSRDR SHDRSRDRDR
210 220 230 240 250
DKERDRDRDR DRDRDRDKDK DRDRDRDRDK ERDRDRDRDR DRERDREGPF
260 270 280 290 300
RRSDSFPERR APRKGNTLYV YGEDMTPTLL RGAFSPFGNI IDLSMDPPRN
310 320 330 340 350
CAFVTYEKME SADQAVAELN GTQVESVQLK VNIARKQPML DAATGKSVWG
360 370
SLAVQNSPKG CHRDKRTQIV YSDDL
Length:375
Mass (Da):42,554
Last modified:November 14, 2003 - v2
Checksum:iE7B5D51A366B7949
GO
Isoform 2 (identifier: P19426-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-231: Missing.

Show »
Length:367
Mass (Da):41,483
Checksum:i7EEE2FB0BA164C7E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18K → E in AAA39680 (PubMed:3353717).Curated1

Polymorphismi

The length of the RD repeats is variable.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti228 – 229Missing in strain: LP.RIII/Sn. 1 Publication2
Natural varianti229 – 236Missing in strain: 129/Sv. 1 Publication8

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008960224 – 231Missing in isoform 2. 2 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21332 Genomic DNA. Translation: AAA39680.1.
AK012389 mRNA. Translation: BAB28206.1.
BC034867 mRNA. Translation: AAH34867.1.
AF109906 Genomic DNA. Translation: AAC84161.1.
U83841 Genomic DNA. Translation: AAB41535.1.
U83842 Genomic DNA. Translation: AAB41536.1.
CCDSiCCDS28662.1. [P19426-2]
PIRiA40112.
RefSeqiNP_001039328.1. NM_001045863.1. [P19426-2]
NP_001039329.1. NM_001045864.1. [P19426-2]
NP_613046.2. NM_138580.2. [P19426-2]
UniGeneiMm.279907.
Mm.653.

Genome annotation databases

EnsembliENSMUST00000097343; ENSMUSP00000094956; ENSMUSG00000024369. [P19426-2]
ENSMUST00000165953; ENSMUSP00000131195; ENSMUSG00000024369. [P19426-2]
ENSMUST00000173357; ENSMUSP00000134272; ENSMUSG00000024369. [P19426-2]
GeneIDi27632.
KEGGimmu:27632.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21332 Genomic DNA. Translation: AAA39680.1.
AK012389 mRNA. Translation: BAB28206.1.
BC034867 mRNA. Translation: AAH34867.1.
AF109906 Genomic DNA. Translation: AAC84161.1.
U83841 Genomic DNA. Translation: AAB41535.1.
U83842 Genomic DNA. Translation: AAB41536.1.
CCDSiCCDS28662.1. [P19426-2]
PIRiA40112.
RefSeqiNP_001039328.1. NM_001045863.1. [P19426-2]
NP_001039329.1. NM_001045864.1. [P19426-2]
NP_613046.2. NM_138580.2. [P19426-2]
UniGeneiMm.279907.
Mm.653.

3D structure databases

ProteinModelPortaliP19426.
SMRiP19426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19426. 1 interactor.
STRINGi10090.ENSMUSP00000094956.

PTM databases

iPTMnetiP19426.
PhosphoSitePlusiP19426.

Proteomic databases

MaxQBiP19426.
PaxDbiP19426.
PeptideAtlasiP19426.
PRIDEiP19426.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097343; ENSMUSP00000094956; ENSMUSG00000024369. [P19426-2]
ENSMUST00000165953; ENSMUSP00000131195; ENSMUSG00000024369. [P19426-2]
ENSMUST00000173357; ENSMUSP00000134272; ENSMUSG00000024369. [P19426-2]
GeneIDi27632.
KEGGimmu:27632.

Organism-specific databases

CTDi7936.
MGIiMGI:102744. Nelfe.

Phylogenomic databases

eggNOGiENOG410IK4K. Eukaryota.
ENOG41101UX. LUCA.
GeneTreeiENSGT00630000089917.
HOGENOMiHOG000232089.
HOVERGENiHBG052598.
InParanoidiP19426.
KOiK15182.
OMAiVYIHFPS.
PhylomeDBiP19426.
TreeFamiTF324087.

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-112387. Elongation arrest and recovery.
R-MMU-113418. Formation of the Early Elongation Complex.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-MMU-75955. RNA Polymerase II Transcription Elongation.

Miscellaneous databases

PROiP19426.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024369.
CleanExiMM_RDBP.
ExpressionAtlasiP19426. baseline and differential.
GenevisibleiP19426. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033102. NELFE.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR17250:SF0. PTHR17250:SF0. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNELFE_MOUSE
AccessioniPrimary (citable) accession number: P19426
Secondary accession number(s): P97485, P97486, Q9CZN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 14, 2003
Last modified: November 30, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.