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Protein

Endoglucanase

Gene
N/A
Organism
Bacillus sp. (strain KSM-635)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei373Proton donor1
Active sitei485Nucleophile1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM17. Carbohydrate-Binding Module Family 17.
CBM28. Carbohydrate-Binding Module Family 28.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Alkaline cellulase
Endo-1,4-beta-glucanase
OrganismiBacillus sp. (strain KSM-635)
Taxonomic identifieri1415 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Add BLAST29
ChainiPRO_000000783830 – 941EndoglucanaseAdd BLAST912

Structurei

Secondary structure

1941
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi232 – 235Combined sources4
Beta strandi239 – 243Combined sources5
Beta strandi246 – 250Combined sources5
Beta strandi254 – 256Combined sources3
Beta strandi260 – 265Combined sources6
Helixi267 – 270Combined sources4
Helixi271 – 273Combined sources3
Helixi276 – 283Combined sources8
Beta strandi289 – 301Combined sources13
Turni302 – 304Combined sources3
Helixi308 – 321Combined sources14
Beta strandi325 – 331Combined sources7
Beta strandi334 – 336Combined sources3
Helixi341 – 343Combined sources3
Helixi346 – 357Combined sources12
Helixi363 – 365Combined sources3
Beta strandi366 – 369Combined sources4
Helixi388 – 408Combined sources21
Beta strandi413 – 415Combined sources3
Helixi418 – 421Combined sources4
Helixi424 – 429Combined sources6
Beta strandi433 – 444Combined sources12
Turni445 – 447Combined sources3
Helixi461 – 463Combined sources3
Helixi467 – 476Combined sources10
Beta strandi481 – 490Combined sources10
Turni491 – 493Combined sources3
Helixi499 – 511Combined sources13
Beta strandi516 – 521Combined sources6
Beta strandi524 – 526Combined sources3
Turni536 – 538Combined sources3
Helixi554 – 556Combined sources3
Helixi559 – 569Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G01X-ray1.90A228-584[»]
1G0CX-ray1.90A228-584[»]
ProteinModelPortaliP19424.
SMRiP19424.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19424.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 94SLH 1PROSITE-ProRule annotationAdd BLAST58
Domaini95 – 158SLH 2PROSITE-ProRule annotationAdd BLAST64
Domaini161 – 224SLH 3PROSITE-ProRule annotationAdd BLAST64

Sequence similaritiesi

Contains 3 SLH (S-layer homology) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR005086. CBM_fam_17/28.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR001119. SLH_dom.
[Graphical view]
PfamiPF03424. CBM_17_28. 2 hits.
PF00150. Cellulase. 1 hit.
PF00395. SLH. 3 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51272. SLH. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIKQIKQSL SLLLIITLIM SLFVPMASAN TNESKSNAFP FSDVKKTSWS
60 70 80 90 100
FPYIKDLYEQ EVITGTSATT FSPTDSVTRA QFTVMLTRGL GLEASSKDYP
110 120 130 140 150
FKDRKNWAYK EIQAAYEAGI VTGKTNGEFA PNENITREQM AAMAVRAYEY
160 170 180 190 200
LENELSLPEE QREYNDSSSI STFAQDAVQK AYVLELMEGN TDGYFQPKRN
210 220 230 240 250
STREQSAKVI STLLWKVASH DYLYHTEAVK SPSEAGALQL VELNGQLTLA
260 270 280 290 300
GEDGTPVQLR GMSTHGLQWF GEIVNENAFV ALSNDWGSNM IRLAMYIGEN
310 320 330 340 350
GYATNPEVKD LVYEGIELAF EHDMYVIVDW HVHAPGDPRA DVYSGAYDFF
360 370 380 390 400
EEIADHYKDH PKNHYIIWEL ANEPSPNNNG GPGLTNDEKG WEAVKEYAEP
410 420 430 440 450
IVEMLREKGD NMILVGNPNW SQRPDLSADN PIDAENIMYS VHFYTGSHGA
460 470 480 490 500
SHIGYPEGTP SSERSNVMAN VRYALDNGVA VFATEWGTSQ ANGDGGPYFD
510 520 530 540 550
EADVWLNFLN KHNISWANWS LTNKNEISGA FTPFELGRTD ATDLDPGANQ
560 570 580 590 600
VWAPEELSLS GEYVRARIKG IEYTPIDRTK FTKLVWDFND GTTQGFQVNG
610 620 630 640 650
DSPNKESITL SNNNDALQIE GLNVSNDISE GNYWDNVRLS ADGWSENVDI
660 670 680 690 700
LGATELTIDV IVEEPTTVSI AAIPQGPAAG WANPTRAIKV TEDDFESFGD
710 720 730 740 750
GYKALVTITS EDSPSLETIA TSPEDNTMSN IILFVGTEDA DVISLDNITV
760 770 780 790 800
SGTEIEIEVI HDEKGTATLP STFEDGTRQG WDWHTESGVK TALTIEEANG
810 820 830 840 850
SNALSWEYAY PEVKPSDGWA TAPRLDFWKD ELVRGTSDYI SFDFYIDAVR
860 870 880 890 900
ASEGAISINA VFQPPANGYW QEVPTTFEID LTELDSATVT SDELYHYEVK
910 920 930 940
INIRDIEAIT DDTELRNLLL IFADEDSDFA GRVFVDNVRF E
Length:941
Mass (Da):104,628
Last modified:November 1, 1990 - v1
Checksum:iBEA2AC3B169BFADA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27420 Genomic DNA. Translation: AAA22304.1.
PIRiS29043.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27420 Genomic DNA. Translation: AAA22304.1.
PIRiS29043.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G01X-ray1.90A228-584[»]
1G0CX-ray1.90A228-584[»]
ProteinModelPortaliP19424.
SMRiP19424.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM17. Carbohydrate-Binding Module Family 17.
CBM28. Carbohydrate-Binding Module Family 28.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP19424.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR005086. CBM_fam_17/28.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR001119. SLH_dom.
[Graphical view]
PfamiPF03424. CBM_17_28. 2 hits.
PF00150. Cellulase. 1 hit.
PF00395. SLH. 3 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51272. SLH. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUN_BACS6
AccessioniPrimary (citable) accession number: P19424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.