Reviewed, UniProtKB/Swiss-Prot P19424 (GUN_BACS6)

Names and origin

Protein names	Recommended name:     Endoglucanase     EC=3.2.1.4 Alternative name(s):     Endo-1,4-beta-glucanase     Alkaline cellulase
Organism	Bacillus sp. (strain KSM-635)
Taxonomic identifier	1415 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	941 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 3 SLH (S-layer homology) domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 29

29

Chain

30 – 941

912

Endoglucanase

PRO_0000007838

Regions

Domain

37 – 94

58

SLH 1

Domain

95 – 158

64

SLH 2

Domain

161 – 224

64

SLH 3

Sites

Active site

373

1

Proton donor

Active site

485

1

Nucleophile

Secondary structure

1

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941

Helix Strand Turn

Details...

Helix

232 – 235

4

Beta strand

239 – 243

5

Beta strand

246 – 250

5

Beta strand

260 – 265

6

Helix

267 – 270

4

Helix

271 – 273

3

Helix

276 – 283

8

Beta strand

289 – 301

13

Turn

302 – 304

3

Helix

308 – 321

14

Beta strand

325 – 331

7

Beta strand

334 – 336

3

Helix

341 – 343

3

Helix

346 – 357

12

Helix

363 – 365

3

Beta strand

366 – 369

4

Helix

388 – 408

21

Beta strand

413 – 415

3

Helix

418 – 421

4

Helix

424 – 429

6

Beta strand

433 – 444

12

Turn

445 – 447

3

Helix

461 – 463

3

Helix

467 – 476

10

Beta strand

481 – 490

10

Turn

491 – 493

3

Helix

499 – 511

13

Beta strand

516 – 521

6

Beta strand

524 – 526

3

Helix

554 – 556

3

Helix

559 – 569

11

Sequences

	Sequence		Length	Mass (Da)
	P19424-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: BEA2AC3B169BFADA	FASTA	941	104,628
	10 20 30 40 50 60 MKIKQIKQSL SLLLIITLIM SLFVPMASAN TNESKSNAFP FSDVKKTSWS FPYIKDLYEQ 70 80 90 100 110 120 EVITGTSATT FSPTDSVTRA QFTVMLTRGL GLEASSKDYP FKDRKNWAYK EIQAAYEAGI 130 140 150 160 170 180 VTGKTNGEFA PNENITREQM AAMAVRAYEY LENELSLPEE QREYNDSSSI STFAQDAVQK 190 200 210 220 230 240 AYVLELMEGN TDGYFQPKRN STREQSAKVI STLLWKVASH DYLYHTEAVK SPSEAGALQL 250 260 270 280 290 300 VELNGQLTLA GEDGTPVQLR GMSTHGLQWF GEIVNENAFV ALSNDWGSNM IRLAMYIGEN 310 320 330 340 350 360 GYATNPEVKD LVYEGIELAF EHDMYVIVDW HVHAPGDPRA DVYSGAYDFF EEIADHYKDH 370 380 390 400 410 420 PKNHYIIWEL ANEPSPNNNG GPGLTNDEKG WEAVKEYAEP IVEMLREKGD NMILVGNPNW 430 440 450 460 470 480 SQRPDLSADN PIDAENIMYS VHFYTGSHGA SHIGYPEGTP SSERSNVMAN VRYALDNGVA 490 500 510 520 530 540 VFATEWGTSQ ANGDGGPYFD EADVWLNFLN KHNISWANWS LTNKNEISGA FTPFELGRTD 550 560 570 580 590 600 ATDLDPGANQ VWAPEELSLS GEYVRARIKG IEYTPIDRTK FTKLVWDFND GTTQGFQVNG 610 620 630 640 650 660 DSPNKESITL SNNNDALQIE GLNVSNDISE GNYWDNVRLS ADGWSENVDI LGATELTIDV 670 680 690 700 710 720 IVEEPTTVSI AAIPQGPAAG WANPTRAIKV TEDDFESFGD GYKALVTITS EDSPSLETIA 730 740 750 760 770 780 TSPEDNTMSN IILFVGTEDA DVISLDNITV SGTEIEIEVI HDEKGTATLP STFEDGTRQG 790 800 810 820 830 840 WDWHTESGVK TALTIEEANG SNALSWEYAY PEVKPSDGWA TAPRLDFWKD ELVRGTSDYI 850 860 870 880 890 900 SFDFYIDAVR ASEGAISINA VFQPPANGYW QEVPTTFEID LTELDSATVT SDELYHYEVK 910 920 930 940 INIRDIEAIT DDTELRNLLL IFADEDSDFA GRVFVDNVRF E

References

[1]	"Molecular cloning and nucleotide sequence of a gene for alkaline cellulase from Bacillus sp. KSM-635." Ozaki K., Shikata S., Kawai S., Ito S., Okamoto K. J. Gen. Microbiol. 136:1327-1334(1990) [PubMed: 2230718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Crystallization and preliminary X-ray analysis of a truncated family A alkaline endoglucanase isolated from Bacillus sp. KSM-635." Shirai T., Yamane T., Hidaka T., Kuyama K., Suzuki A., Ashida T., Ozaki K., Ito S. J. Biochem. 122:683-685(1997) [PubMed: 9399567] [Abstract] Cited for: PROTEIN SEQUENCE OF 228-584, CRYSTALLIZATION.
[3]	"Crystal structure of alkaline cellulase K: insight into the alkaline adaptation of an industrial enzyme." Shirai T., Ishida H., Noda J., Yamane T., Ozaki K., Hakamada Y., Ito S. J. Mol. Biol. 310:1079-1087(2001) [PubMed: 11501997] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 221-584.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M27420 Genomic DNA. Translation: AAA22304.1.

PIR

S29043.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G01	X-ray	1.90	A	228-584	[»]
1G0C	X-ray	1.90	A	228-584	[»]

SMR

P19424. Positions 759-941.

ModBase

Search...

Protein family/group databases

CAZy

CBM17. Carbohydrate-Binding Module Family 17.
CBM28. Carbohydrate-Binding Module Family 28.
GH5. Glycoside Hydrolase Family 5.

Family and domain databases

InterPro

IPR005086. CBM_17_28.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_sg_catalytic.
IPR001119. SLH.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

Pfam

PF03424. CBM_17_28. 2 hits.
PF00150. Cellulase. 1 hit.
PF00395. SLH. 3 hits.
[Graphical view]

PROSITE

PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51272. SLH. 3 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GUN_BACS6

Accession

Primary (citable) accession number: P19424

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)