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P19424

- GUN_BACS6

UniProt

P19424 - GUN_BACS6

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Protein
Endoglucanase
Gene
N/A
Organism
Bacillus sp. (strain KSM-635)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei373 – 3731Proton donor
Active sitei485 – 4851Nucleophile

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM17. Carbohydrate-Binding Module Family 17.
CBM28. Carbohydrate-Binding Module Family 28.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Alkaline cellulase
Endo-1,4-beta-glucanase
OrganismiBacillus sp. (strain KSM-635)
Taxonomic identifieri1415 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929
Add
BLAST
Chaini30 – 941912Endoglucanase
PRO_0000007838Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi232 – 2354
Beta strandi239 – 2435
Beta strandi246 – 2505
Beta strandi254 – 2563
Beta strandi260 – 2656
Helixi267 – 2704
Helixi271 – 2733
Helixi276 – 2838
Beta strandi289 – 30113
Turni302 – 3043
Helixi308 – 32114
Beta strandi325 – 3317
Beta strandi334 – 3363
Helixi341 – 3433
Helixi346 – 35712
Helixi363 – 3653
Beta strandi366 – 3694
Helixi388 – 40821
Beta strandi413 – 4153
Helixi418 – 4214
Helixi424 – 4296
Beta strandi433 – 44412
Turni445 – 4473
Helixi461 – 4633
Helixi467 – 47610
Beta strandi481 – 49010
Turni491 – 4933
Helixi499 – 51113
Beta strandi516 – 5216
Beta strandi524 – 5263
Turni536 – 5383
Helixi554 – 5563
Helixi559 – 56911

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G01X-ray1.90A228-584[»]
1G0CX-ray1.90A228-584[»]
ProteinModelPortaliP19424.
SMRiP19424. Positions 227-752, 759-941.

Miscellaneous databases

EvolutionaryTraceiP19424.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 9458SLH 1
Add
BLAST
Domaini95 – 15864SLH 2
Add
BLAST
Domaini161 – 22464SLH 3
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR005086. CBM_fam_17/28.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR001119. S-layer_homology_dom.
[Graphical view]
PfamiPF03424. CBM_17_28. 2 hits.
PF00150. Cellulase. 1 hit.
PF00395. SLH. 3 hits.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51272. SLH. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19424-1 [UniParc]FASTAAdd to Basket

« Hide

MKIKQIKQSL SLLLIITLIM SLFVPMASAN TNESKSNAFP FSDVKKTSWS    50
FPYIKDLYEQ EVITGTSATT FSPTDSVTRA QFTVMLTRGL GLEASSKDYP 100
FKDRKNWAYK EIQAAYEAGI VTGKTNGEFA PNENITREQM AAMAVRAYEY 150
LENELSLPEE QREYNDSSSI STFAQDAVQK AYVLELMEGN TDGYFQPKRN 200
STREQSAKVI STLLWKVASH DYLYHTEAVK SPSEAGALQL VELNGQLTLA 250
GEDGTPVQLR GMSTHGLQWF GEIVNENAFV ALSNDWGSNM IRLAMYIGEN 300
GYATNPEVKD LVYEGIELAF EHDMYVIVDW HVHAPGDPRA DVYSGAYDFF 350
EEIADHYKDH PKNHYIIWEL ANEPSPNNNG GPGLTNDEKG WEAVKEYAEP 400
IVEMLREKGD NMILVGNPNW SQRPDLSADN PIDAENIMYS VHFYTGSHGA 450
SHIGYPEGTP SSERSNVMAN VRYALDNGVA VFATEWGTSQ ANGDGGPYFD 500
EADVWLNFLN KHNISWANWS LTNKNEISGA FTPFELGRTD ATDLDPGANQ 550
VWAPEELSLS GEYVRARIKG IEYTPIDRTK FTKLVWDFND GTTQGFQVNG 600
DSPNKESITL SNNNDALQIE GLNVSNDISE GNYWDNVRLS ADGWSENVDI 650
LGATELTIDV IVEEPTTVSI AAIPQGPAAG WANPTRAIKV TEDDFESFGD 700
GYKALVTITS EDSPSLETIA TSPEDNTMSN IILFVGTEDA DVISLDNITV 750
SGTEIEIEVI HDEKGTATLP STFEDGTRQG WDWHTESGVK TALTIEEANG 800
SNALSWEYAY PEVKPSDGWA TAPRLDFWKD ELVRGTSDYI SFDFYIDAVR 850
ASEGAISINA VFQPPANGYW QEVPTTFEID LTELDSATVT SDELYHYEVK 900
INIRDIEAIT DDTELRNLLL IFADEDSDFA GRVFVDNVRF E 941
Length:941
Mass (Da):104,628
Last modified:November 1, 1990 - v1
Checksum:iBEA2AC3B169BFADA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27420 Genomic DNA. Translation: AAA22304.1.
PIRiS29043.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27420 Genomic DNA. Translation: AAA22304.1 .
PIRi S29043.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G01 X-ray 1.90 A 228-584 [» ]
1G0C X-ray 1.90 A 228-584 [» ]
ProteinModelPortali P19424.
SMRi P19424. Positions 227-752, 759-941.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM17. Carbohydrate-Binding Module Family 17.
CBM28. Carbohydrate-Binding Module Family 28.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P19424.

Family and domain databases

Gene3Di 2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProi IPR005086. CBM_fam_17/28.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR001119. S-layer_homology_dom.
[Graphical view ]
Pfami PF03424. CBM_17_28. 2 hits.
PF00150. Cellulase. 1 hit.
PF00395. SLH. 3 hits.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51272. SLH. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and nucleotide sequence of a gene for alkaline cellulase from Bacillus sp. KSM-635."
    Ozaki K., Shikata S., Kawai S., Ito S., Okamoto K.
    J. Gen. Microbiol. 136:1327-1334(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Crystallization and preliminary X-ray analysis of a truncated family A alkaline endoglucanase isolated from Bacillus sp. KSM-635."
    Shirai T., Yamane T., Hidaka T., Kuyama K., Suzuki A., Ashida T., Ozaki K., Ito S.
    J. Biochem. 122:683-685(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 228-584, CRYSTALLIZATION.
  3. "Crystal structure of alkaline cellulase K: insight into the alkaline adaptation of an industrial enzyme."
    Shirai T., Ishida H., Noda J., Yamane T., Ozaki K., Hakamada Y., Ito S.
    J. Mol. Biol. 310:1079-1087(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 221-584.

Entry informationi

Entry nameiGUN_BACS6
AccessioniPrimary (citable) accession number: P19424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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