Reviewed,
UniProtKB/Swiss-Prot P19414 (ACON_YEAST)
Last modified
November 3, 2009.
Version 98.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Aconitate hydratase, mitochondrial Short name=Aconitase EC=4.2.1.3 Alternative name(s): Citrate hydro-lyase | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 778 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Required for growth on nonfermentable carbon sources and for biosynthesis of glutamate. |
| Catalytic activity | Citrate = isocitrate. |
| Cofactor | Binds 1 4Fe-4S cluster per subunit By similarity. |
| Enzyme regulation | Subject to catabolite regulation. |
| Pathway | |
| Subunit structure | Monomer. |
| Subcellular location | Mitochondrion. Cytoplasm. Note: Mitochondrial and extramitochondrial. |
| Miscellaneous | Present with 96700 molecules/cell in log phase SD medium. Ref.3 |
| Sequence similarities | Belongs to the aconitase/IPM isomerase family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Mitochondrion | |||||||
| Chain | ? – 778 | Aconitate hydratase, mitochondrial | PRO_0000000547 | ||||||
Sites | |||||||||
| Metal binding | 382 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 445 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 448 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 351 | 1 | Phosphothreonine Ref.6 | ||||||
| Modified residue | 409 | 1 | Phosphothreonine Ref.5 | ||||||
| Modified residue | 556 | 1 | Phosphoserine Ref.4 | ||||||
Experimental info | |||||||||
| Sequence conflict | 527 – 549 | 23 | DGLPQ…PADRS → RWFASKEVMMLVRTLTKLHL QTVA Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning of the yeast mitochondrial aconitase gene (ACO1) and evidence of a synergistic regulation of expression by glucose plus glutamate." Gangloff S.P., Marguet D., Lauquin G.J.-M. Mol. Cell. Biol. 10:3551-3561(1990) [PubMed: 1972545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 44774 / DBY747. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D.Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [4] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-556, MASS SPECTROMETRY. |
| [5] | "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-409, MASS SPECTROMETRY. |
| [6] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-351, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M33131 Genomic DNA. Translation: AAA34389.1. U17243 Genomic DNA. Translation: AAB67348.1. | |
| PIR | S50387. |
| RefSeq | NP_013407.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1AMJ based on UniProtKB P20004. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:4679N. |
| IntAct | P19414. 55 interactions. |
| STRING | P19414. |
Proteomic databases | |
| PeptideAtlas | P19414. |
| PRIDE | P19414. |
Genome annotation databases | |
| Ensembl | YLR304C; YLR304C; YLR304C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 851013. |
| GenomeReviews | Gene locus YLR304C in contig Y13138_GR. |
| KEGG | sce:YLR304C. |
| NMPDR | fig|4932.3.peg.4425. |
Organism-specific databases | |
| CYGD | YLR304c. |
| SGD | S000004295. ACO1. |
Phylogenomic databases | |
| HOGENOM | P19414. |
| OMA | PGKPLKC. |
Enzyme and pathway databases | |
| BRENDA | 4.2.1.3. 250. |
Gene expression databases | |
| ArrayExpress | P19414. |
| Genevestigator | P19414. |
| GermOnline | YLR304C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR006248. Aconitase_mito-like. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view] |
| Gene3D | G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit. |
| PANTHER | PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF5. Aconitase_mito. 1 hit. |
| Pfam | PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view] |
| PRINTS | PR00415. ACONITASE. |
| ProDom | PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR01340. aconitase_mito. 1 hit. |
| PROSITE | PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 967571. |
Entry information
| Entry name | ACON_YEAST | ||||||||
| Accession | Primary (citable) accession number: P19414 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


