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P19406

- PPB4_BACSU

UniProt

P19406 - PPB4_BACSU

Protein

Alkaline phosphatase 4

Gene

phoA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 4 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.
    Binds 2 zinc ions.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi58 – 581MagnesiumBy similarity
    Metal bindingi58 – 581Zinc 2By similarity
    Active sitei108 – 1081Phosphoserine intermediatePROSITE-ProRule annotation
    Metal bindingi161 – 1611MagnesiumBy similarity
    Metal bindingi282 – 2821MagnesiumBy similarity
    Metal bindingi287 – 2871Zinc 1By similarity
    Metal bindingi291 – 2911Zinc 1By similarity
    Metal bindingi329 – 3291Zinc 2By similarity
    Metal bindingi330 – 3301Zinc 2By similarity
    Metal bindingi423 – 4231Zinc 1By similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciBSUB:BSU09410-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase 4 (EC:3.1.3.1)
    Alternative name(s):
    Alkaline phosphatase IV
    Short name:
    APase IV
    Gene namesi
    Name:phoA
    Synonyms:phoAIV
    Ordered Locus Names:BSU09410
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU09410. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 41411 PublicationAdd
    BLAST
    Chaini42 – 461420Alkaline phosphatase 4PRO_0000024011Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP19406.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi224308.BSU09410.

    Structurei

    3D structure databases

    ProteinModelPortaliP19406.
    SMRiP19406. Positions 51-458.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1785.
    HOGENOMiHOG000099116.
    KOiK01077.
    OrthoDBiEOG661H4G.
    PhylomeDBiP19406.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19406-1 [UniParc]FASTAAdd to Basket

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    MKKMSLFQNM KSKLLPIAAV SVLTAGIFAG AELQQTEKAS AKKQDKAEIR    50
    NVIVMIGDGM GTPYIRAYRS MKNNGDTPNN PKLTEFDRNL TGMMMTHPDD 100
    PDYNITDSAA AGTALATGVK TYNNAIGVDK NGKKVKSVLE EAKQQGKSTG 150
    LVATSEINHA TPAAYGAHNE SRKNMDQIAN SYMDDKIKGK HKIDVLLGGG 200
    KSYFNRKDRN LTKEFKQAGY SYVTTKQALK KNKDQQVLGL FADGGLAKAL 250
    DRDSKTPSLK DMTVSAIDRL NQNKKGFFLM VEGSQIDWAA HDNDTVGAMS 300
    EVKDFEQAYK AAIEFAKKDK HTLVIATADH TTGGFTIGAN GEKNWHAEPI 350
    LSAKKTPEFM AKKISEGKPV KDVLARYANL KVTSEEIKSV EAAAQADKSK 400
    GASKAIIKIF NTRSNSGWTS TDHTGEEVPV YAYGPGKEKF RGLINNTDQA 450
    NIIFKILKTG K 461
    Length:461
    Mass (Da):50,274
    Last modified:June 16, 2009 - v4
    Checksum:iA2AD9309026889FE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti50 – 501R → K AA sequence (PubMed:2105301)Curated
    Sequence conflicti208 – 2081D → N in AAA18323. (PubMed:8113174)Curated
    Sequence conflicti208 – 2081D → N in CAA74486. (PubMed:9579061)Curated
    Sequence conflicti208 – 2081D → N(PubMed:1898729)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02550 Unassigned DNA. Translation: AAA18323.1.
    Y14082 Genomic DNA. Translation: CAA74486.1.
    AL009126 Genomic DNA. Translation: CAB12780.2.
    L26337 Genomic DNA. Translation: AAA22812.1.
    PIRiB69676.
    RefSeqiNP_388822.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB12780; CAB12780; BSU09410.
    GeneIDi936265.
    KEGGibsu:BSU09410.
    PATRICi18973578. VBIBacSub10457_0983.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02550 Unassigned DNA. Translation: AAA18323.1 .
    Y14082 Genomic DNA. Translation: CAA74486.1 .
    AL009126 Genomic DNA. Translation: CAB12780.2 .
    L26337 Genomic DNA. Translation: AAA22812.1 .
    PIRi B69676.
    RefSeqi NP_388822.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali P19406.
    SMRi P19406. Positions 51-458.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU09410.

    Proteomic databases

    PaxDbi P19406.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12780 ; CAB12780 ; BSU09410 .
    GeneIDi 936265.
    KEGGi bsu:BSU09410.
    PATRICi 18973578. VBIBacSub10457_0983.

    Organism-specific databases

    GenoListi BSU09410. [Micado ]

    Phylogenomic databases

    eggNOGi COG1785.
    HOGENOMi HOG000099116.
    KOi K01077.
    OrthoDBi EOG661H4G.
    PhylomeDBi P19406.

    Enzyme and pathway databases

    BioCyci BSUB:BSU09410-MONOMER.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequential action of two-component genetic switches regulates the PHO regulon in Bacillus subtilis."
      Hulett F.M., Lee J., Shi L., Sun G., Chesnut R., Sharkova E., Duggan M.F., Kapp N.
      J. Bacteriol. 176:1348-1358(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    2. "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene."
      Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S.
      Microbiology 144:859-875(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 208.
    5. "Bacillus subtilis alkaline phosphatases III and IV. Cloning, sequencing, and comparisons of deduced amino acid sequence with Escherichia coli alkaline phosphatase three-dimensional structure."
      Hulett F.M., Kim M.E., Bookstein C., Kapp N.V., Edwards C.W., Wyckoff H.W.
      J. Biol. Chem. 266:1077-1084(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 39-461.
      Strain: 168 / JH642.
    6. "The Bacillus subtilis phoAIV gene: effects of in vitro inactivation on total alkaline phosphatase production."
      Kapp N.V., Edwards C.W., Chesnut R.S., Hulett F.M.
      Gene 96:95-100(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 39-142.
      Strain: 168 / JH642.
    7. "Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis."
      Hulett F.M., Bookstein C., Jensen K.
      J. Bacteriol. 172:735-740(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 42-63.
    8. "Cloning and characterization of spoVR, a gene from Bacillus subtilis involved in spore cortex formation."
      Beall B.W., Moran C.P. Jr.
      J. Bacteriol. 176:2003-2012(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 433-461.
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.

    Entry informationi

    Entry nameiPPB4_BACSU
    AccessioniPrimary (citable) accession number: P19406
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 117 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3