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Protein

Alkaline phosphatase 4

Gene

phoA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg2+ ion.
  • Zn2+Note: Binds 2 Zn2+ ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58MagnesiumBy similarity1
Metal bindingi58Zinc 2By similarity1
Active sitei108Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi161MagnesiumBy similarity1
Metal bindingi282MagnesiumBy similarity1
Metal bindingi287Zinc 1By similarity1
Metal bindingi291Zinc 1By similarity1
Metal bindingi329Zinc 2By similarity1
Metal bindingi330Zinc 2By similarity1
Metal bindingi423Zinc 1By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU09410-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase 4 (EC:3.1.3.1)
Alternative name(s):
Alkaline phosphatase IV
Short name:
APase IV
Gene namesi
Name:phoA
Synonyms:phoAIV
Ordered Locus Names:BSU09410
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 411 PublicationAdd BLAST41
ChainiPRO_000002401142 – 461Alkaline phosphatase 4Add BLAST420

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP19406.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005193.

Structurei

3D structure databases

ProteinModelPortaliP19406.
SMRiP19406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105G6Z. Bacteria.
COG1785. LUCA.
HOGENOMiHOG000099116.
InParanoidiP19406.
KOiK01077.
OMAiNLTGMMM.
PhylomeDBiP19406.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKMSLFQNM KSKLLPIAAV SVLTAGIFAG AELQQTEKAS AKKQDKAEIR
60 70 80 90 100
NVIVMIGDGM GTPYIRAYRS MKNNGDTPNN PKLTEFDRNL TGMMMTHPDD
110 120 130 140 150
PDYNITDSAA AGTALATGVK TYNNAIGVDK NGKKVKSVLE EAKQQGKSTG
160 170 180 190 200
LVATSEINHA TPAAYGAHNE SRKNMDQIAN SYMDDKIKGK HKIDVLLGGG
210 220 230 240 250
KSYFNRKDRN LTKEFKQAGY SYVTTKQALK KNKDQQVLGL FADGGLAKAL
260 270 280 290 300
DRDSKTPSLK DMTVSAIDRL NQNKKGFFLM VEGSQIDWAA HDNDTVGAMS
310 320 330 340 350
EVKDFEQAYK AAIEFAKKDK HTLVIATADH TTGGFTIGAN GEKNWHAEPI
360 370 380 390 400
LSAKKTPEFM AKKISEGKPV KDVLARYANL KVTSEEIKSV EAAAQADKSK
410 420 430 440 450
GASKAIIKIF NTRSNSGWTS TDHTGEEVPV YAYGPGKEKF RGLINNTDQA
460
NIIFKILKTG K
Length:461
Mass (Da):50,274
Last modified:June 16, 2009 - v4
Checksum:iA2AD9309026889FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50R → K AA sequence (PubMed:2105301).Curated1
Sequence conflicti208D → N in AAA18323 (PubMed:8113174).Curated1
Sequence conflicti208D → N in CAA74486 (PubMed:9579061).Curated1
Sequence conflicti208D → N (PubMed:1898729).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02550 Unassigned DNA. Translation: AAA18323.1.
Y14082 Genomic DNA. Translation: CAA74486.1.
AL009126 Genomic DNA. Translation: CAB12780.2.
L26337 Genomic DNA. Translation: AAA22812.1.
PIRiB69676.
RefSeqiNP_388822.2. NC_000964.3.
WP_010886458.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB12780; CAB12780; BSU09410.
GeneIDi936265.
KEGGibsu:BSU09410.
PATRICi18973578. VBIBacSub10457_0983.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02550 Unassigned DNA. Translation: AAA18323.1.
Y14082 Genomic DNA. Translation: CAA74486.1.
AL009126 Genomic DNA. Translation: CAB12780.2.
L26337 Genomic DNA. Translation: AAA22812.1.
PIRiB69676.
RefSeqiNP_388822.2. NC_000964.3.
WP_010886458.1. NC_000964.3.

3D structure databases

ProteinModelPortaliP19406.
SMRiP19406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005193.

Proteomic databases

PaxDbiP19406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12780; CAB12780; BSU09410.
GeneIDi936265.
KEGGibsu:BSU09410.
PATRICi18973578. VBIBacSub10457_0983.

Phylogenomic databases

eggNOGiENOG4105G6Z. Bacteria.
COG1785. LUCA.
HOGENOMiHOG000099116.
InParanoidiP19406.
KOiK01077.
OMAiNLTGMMM.
PhylomeDBiP19406.

Enzyme and pathway databases

BioCyciBSUB:BSU09410-MONOMER.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPB4_BACSU
AccessioniPrimary (citable) accession number: P19406
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: June 16, 2009
Last modified: November 30, 2016
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.