Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alkaline phosphatase 4

Gene

phoA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg2+ ion.
  • Zn2+Note: Binds 2 Zn2+ ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58MagnesiumBy similarity1
Metal bindingi58Zinc 2By similarity1
Active sitei108Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi161MagnesiumBy similarity1
Metal bindingi282MagnesiumBy similarity1
Metal bindingi287Zinc 1By similarity1
Metal bindingi291Zinc 1By similarity1
Metal bindingi329Zinc 2By similarity1
Metal bindingi330Zinc 2By similarity1
Metal bindingi423Zinc 1By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU09410-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase 4 (EC:3.1.3.1)
Alternative name(s):
Alkaline phosphatase IV
Short name:
APase IV
Gene namesi
Name:phoA
Synonyms:phoAIV
Ordered Locus Names:BSU09410
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 411 PublicationAdd BLAST41
ChainiPRO_000002401142 – 461Alkaline phosphatase 4Add BLAST420

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP19406
PRIDEiP19406

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005193

Structurei

3D structure databases

ProteinModelPortaliP19406
SMRiP19406
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105G6Z Bacteria
COG1785 LUCA
HOGENOMiHOG000099116
InParanoidiP19406
KOiK01077
OMAiNLTGMMM
PhylomeDBiP19406

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 2 hits
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 1 hit
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKMSLFQNM KSKLLPIAAV SVLTAGIFAG AELQQTEKAS AKKQDKAEIR
60 70 80 90 100
NVIVMIGDGM GTPYIRAYRS MKNNGDTPNN PKLTEFDRNL TGMMMTHPDD
110 120 130 140 150
PDYNITDSAA AGTALATGVK TYNNAIGVDK NGKKVKSVLE EAKQQGKSTG
160 170 180 190 200
LVATSEINHA TPAAYGAHNE SRKNMDQIAN SYMDDKIKGK HKIDVLLGGG
210 220 230 240 250
KSYFNRKDRN LTKEFKQAGY SYVTTKQALK KNKDQQVLGL FADGGLAKAL
260 270 280 290 300
DRDSKTPSLK DMTVSAIDRL NQNKKGFFLM VEGSQIDWAA HDNDTVGAMS
310 320 330 340 350
EVKDFEQAYK AAIEFAKKDK HTLVIATADH TTGGFTIGAN GEKNWHAEPI
360 370 380 390 400
LSAKKTPEFM AKKISEGKPV KDVLARYANL KVTSEEIKSV EAAAQADKSK
410 420 430 440 450
GASKAIIKIF NTRSNSGWTS TDHTGEEVPV YAYGPGKEKF RGLINNTDQA
460
NIIFKILKTG K
Length:461
Mass (Da):50,274
Last modified:June 16, 2009 - v4
Checksum:iA2AD9309026889FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50R → K AA sequence (PubMed:2105301).Curated1
Sequence conflicti208D → N in AAA18323 (PubMed:8113174).Curated1
Sequence conflicti208D → N in CAA74486 (PubMed:9579061).Curated1
Sequence conflicti208D → N (PubMed:1898729).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02550 Unassigned DNA Translation: AAA18323.1
Y14082 Genomic DNA Translation: CAA74486.1
AL009126 Genomic DNA Translation: CAB12780.2
L26337 Genomic DNA Translation: AAA22812.1
PIRiB69676
RefSeqiNP_388822.2, NC_000964.3
WP_010886458.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB12780; CAB12780; BSU09410
GeneIDi936265
KEGGibsu:BSU09410
PATRICifig|224308.43.peg.983

Similar proteinsi

Entry informationi

Entry nameiPPB4_BACSU
AccessioniPrimary (citable) accession number: P19406
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 139 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health