P19406 (PPB4_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkaline phosphatase 4
EC=3.1.3.1

Alternative name(s):
Alkaline phosphatase IV
Short name=APase IV

Gene names

Name:	phoA
Synonyms:	phoAIV
Ordered Locus Names:	BSU09410

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion. Binds 2 zinc ions.
Subunit structure	Monomer.
Sequence similarities	Belongs to the alkaline phosphatase family.

Ontologies

Keywords
Domain	Signal
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	alkaline phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	Ref.7
	Chain	42 – 461	420	Alkaline phosphatase 4		PRO_0000024011
Sites
	Active site	108	1	Phosphoserine intermediate By similarity
	Metal binding	58	1	Magnesium By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	161	1	Magnesium By similarity
	Metal binding	282	1	Magnesium By similarity
	Metal binding	287	1	Zinc 1 By similarity
	Metal binding	291	1	Zinc 1 By similarity
	Metal binding	329	1	Zinc 2 By similarity
	Metal binding	330	1	Zinc 2 By similarity
	Metal binding	423	1	Zinc 1 By similarity
Experimental info
	Sequence conflict	50	1	R → K AA sequence Ref.7
	Sequence conflict	208	1	D → N in AAA18323. Ref.1
	Sequence conflict	208	1	D → N in CAA74486. Ref.2
	Sequence conflict	208	1	D → N Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P19406 [UniParc]. Last modified June 16, 2009. Version 4. Checksum: A2AD9309026889FE	FASTA	461	50,274
	10 20 30 40 50 60 MKKMSLFQNM KSKLLPIAAV SVLTAGIFAG AELQQTEKAS AKKQDKAEIR NVIVMIGDGM 70 80 90 100 110 120 GTPYIRAYRS MKNNGDTPNN PKLTEFDRNL TGMMMTHPDD PDYNITDSAA AGTALATGVK 130 140 150 160 170 180 TYNNAIGVDK NGKKVKSVLE EAKQQGKSTG LVATSEINHA TPAAYGAHNE SRKNMDQIAN 190 200 210 220 230 240 SYMDDKIKGK HKIDVLLGGG KSYFNRKDRN LTKEFKQAGY SYVTTKQALK KNKDQQVLGL 250 260 270 280 290 300 FADGGLAKAL DRDSKTPSLK DMTVSAIDRL NQNKKGFFLM VEGSQIDWAA HDNDTVGAMS 310 320 330 340 350 360 EVKDFEQAYK AAIEFAKKDK HTLVIATADH TTGGFTIGAN GEKNWHAEPI LSAKKTPEFM 370 380 390 400 410 420 AKKISEGKPV KDVLARYANL KVTSEEIKSV EAAAQADKSK GASKAIIKIF NTRSNSGWTS 430 440 450 460 TDHTGEEVPV YAYGPGKEKF RGLINNTDQA NIIFKILKTG K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequential action of two-component genetic switches regulates the PHO regulon in Bacillus subtilis." Hulett F.M., Lee J., Shi L., Sun G., Chesnut R., Sharkova E., Duggan M.F., Kapp N. J. Bacteriol. 176:1348-1358(1994) [PubMed: 8113174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[2]	"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene." Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S. Microbiology 144:859-875(1998) [PubMed: 9579061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 208.
[5]	"Bacillus subtilis alkaline phosphatases III and IV. Cloning, sequencing, and comparisons of deduced amino acid sequence with Escherichia coli alkaline phosphatase three-dimensional structure." Hulett F.M., Kim M.E., Bookstein C., Kapp N.V., Edwards C.W., Wyckoff H.W. J. Biol. Chem. 266:1077-1084(1991) [PubMed: 1898729] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 39-461. Strain: 168 / JH642.
[6]	"The Bacillus subtilis phoAIV gene: effects of in vitro inactivation on total alkaline phosphatase production." Kapp N.V., Edwards C.W., Chesnut R.S., Hulett F.M. Gene 96:95-100(1990) [PubMed: 2125017] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 39-142. Strain: 168 / JH642.
[7]	"Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis." Hulett F.M., Bookstein C., Jensen K. J. Bacteriol. 172:735-740(1990) [PubMed: 2105301] [Abstract] Cited for: PROTEIN SEQUENCE OF 42-63.
[8]	"Cloning and characterization of spoVR, a gene from Bacillus subtilis involved in spore cortex formation." Beall B.W., Moran C.P. Jr. J. Bacteriol. 176:2003-2012(1994) [PubMed: 8144469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 433-461. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U02550 Unassigned DNA. Translation: AAA18323.1. Y14082 Genomic DNA. Translation: CAA74486.1. AL009126 Genomic DNA. Translation: CAB12780.2. L26337 Genomic DNA. Translation: AAA22812.1.
PIR	B69676.
RefSeq	NP_388822.2. NC_000964.3.
3D structure databases
ProteinModelPortal	P19406.
SMR	P19406. Positions 51-458.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000003817; EBBACP00000003817; EBBACG00000003809.
GeneID	936265.
GenomeReviews	Gene locus BSU09410 in contig AL009126_GR.
KEGG	bsu:BSU09410.
NMPDR	fig|224308.1.peg.941.
PATRIC	18973578. VBIBacSub10457_0983.
Organism-specific databases
GenoList	BSU09410. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000002942.
HOGENOM	HBG617166.
PhylomeDB	P19406.
ProtClustDB	CLSK2460902.
Enzyme and pathway databases
BioCyc	BSUB:BSU09410-MONOMER.
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. IPR017850. Alkaline_phosphatase_core. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
KO	K01077.
Pfam	PF00245. Alk_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00113. ALKPHPHTASE.
SMART	SM00098. alkPPc. 1 hit. [Graphical view]
SUPFAM	SSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITE	PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPB4_BACSU

Accession

Primary (citable) accession number: P19406

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 102 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program