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P19405

- PPB3_BACSU

UniProt

P19405 - PPB3_BACSU

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Protein

Alkaline phosphatase 3

Gene
phoB, phoAIII, BSU05740
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 magnesium ion.
Binds 2 zinc ions.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521Magnesium By similarity
Metal bindingi52 – 521Zinc 2 By similarity
Active sitei101 – 1011Phosphoserine intermediate By similarity
Metal bindingi154 – 1541Magnesium By similarity
Metal bindingi275 – 2751Magnesium By similarity
Metal bindingi280 – 2801Zinc 1 By similarity
Metal bindingi284 – 2841Zinc 1 By similarity
Metal bindingi322 – 3221Zinc 2 By similarity
Metal bindingi323 – 3231Zinc 2 By similarity
Metal bindingi419 – 4191Zinc 1 By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU05740-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase 3 (EC:3.1.3.1)
Alternative name(s):
Alkaline phosphatase III
Short name:
APase III
Gene namesi
Name:phoB
Synonyms:phoAIII
Ordered Locus Names:BSU05740
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU05740. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 32321 PublicationAdd
BLAST
Chaini33 – 462430Alkaline phosphatase 3PRO_0000024010Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP19405.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi224308.BSU05740.

Structurei

3D structure databases

ProteinModelPortaliP19405.
SMRiP19405. Positions 45-454.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099116.
KOiK01077.
OrthoDBiEOG661H4G.
PhylomeDBiP19405.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19405-1 [UniParc]FASTAAdd to Basket

« Hide

MKKFPKKLLP IAVLSSIAFS SLASGSVPEA SAQEKKKGNQ DEIKNVIVLI    50
GDGMGVSYTS AYRYLKDNKK TKVVEPTAFD QYLVGQQTTY PDDPEQNVTD 100
SAAAATAMSA GIKTYNNAIA VDNDGSEAKT VLEAAKEKGK ATGLVATSEI 150
THATPASFGS HDHSRKNMNS IADDYFDEMV NGKHKIDVLL GGGKSNFDRK 200
DRNLIKEFKK AGYSYVDDRK DMLKNKDSQV LGLFADGGLP KKIDRTKDIP 250
SLKDMTNTAI KKLNKDKDGF FLMVEGSQID WAGHDNDIVG AMSEMEDFEQ 300
AYKAAIDFAK KDKHTLVVAT ADHSTGGYSI GADGIYNWFS EPIKAAKRTP 350
DFMAEKIADG ADVEKTLKTY IDQKKLALTK AEIQSVEEAA KSKEVLDIDN 400
AIENIFNKRS HTGWTTGGHT GEDVPVYAYG PSSETFAGQI DNTEIAKNVF 450
KALQYNIKIN DK 462
Length:462
Mass (Da):50,494
Last modified:July 7, 2009 - v4
Checksum:iCB0F8FB855D17231
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti215 – 2151Y → S1 Publication
Sequence conflicti234 – 2352FA → LP1 Publication
Sequence conflicti334 – 3341G → S in BAA19698. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D88802 Genomic DNA. Translation: BAA19698.1.
AL009126 Genomic DNA. Translation: CAB12393.2.
M33634 Genomic DNA. Translation: AAA22658.1.
PIRiC69676.
RefSeqiNP_388455.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB12393; CAB12393; BSU05740.
GeneIDi938004.
KEGGibsu:BSU05740.
PATRICi18972762. VBIBacSub10457_0602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D88802 Genomic DNA. Translation: BAA19698.1 .
AL009126 Genomic DNA. Translation: CAB12393.2 .
M33634 Genomic DNA. Translation: AAA22658.1 .
PIRi C69676.
RefSeqi NP_388455.2. NC_000964.3.

3D structure databases

ProteinModelPortali P19405.
SMRi P19405. Positions 45-454.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU05740.

Proteomic databases

PaxDbi P19405.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB12393 ; CAB12393 ; BSU05740 .
GeneIDi 938004.
KEGGi bsu:BSU05740.
PATRICi 18972762. VBIBacSub10457_0602.

Organism-specific databases

GenoListi BSU05740. [Micado ]

Phylogenomic databases

eggNOGi COG1785.
HOGENOMi HOG000099116.
KOi K01077.
OrthoDBi EOG661H4G.
PhylomeDBi P19405.

Enzyme and pathway databases

BioCyci BSUB:BSU05740-MONOMER.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bacillus subtilis alkaline phosphatases III and IV. Cloning, sequencing, and comparisons of deduced amino acid sequence with Escherichia coli alkaline phosphatase three-dimensional structure."
    Hulett F.M., Kim M.E., Bookstein C., Kapp N.V., Edwards C.W., Wyckoff H.W.
    J. Biol. Chem. 266:1077-1084(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome."
    Sadaie Y., Yata K., Fujita M., Sagai H., Itaya M., Kasahara Y., Ogasawara N.
    Microbiology 143:1861-1866(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 334.
  5. "The Bacillus subtilis 168 alkaline phosphatase III gene: impact of a phoAIII mutation on total alkaline phosphatase synthesis."
    Bookstein C., Edwards C.W., Kapp N.V., Hulett F.M.
    J. Bacteriol. 172:3730-3737(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-80.
    Strain: 168.
  6. "Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis."
    Hulett F.M., Bookstein C., Jensen K.
    J. Bacteriol. 172:735-740(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 33-62.

Entry informationi

Entry nameiPPB3_BACSU
AccessioniPrimary (citable) accession number: P19405
Secondary accession number(s): O05498
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 7, 2009
Last modified: July 9, 2014
This is version 112 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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