Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alkaline phosphatase 3

Gene

phoB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg2+ ion.
  • Zn2+Note: Binds 2 Zn2+ ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52MagnesiumBy similarity1
Metal bindingi52Zinc 2By similarity1
Active sitei101Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi154MagnesiumBy similarity1
Metal bindingi275MagnesiumBy similarity1
Metal bindingi280Zinc 1By similarity1
Metal bindingi284Zinc 1By similarity1
Metal bindingi322Zinc 2By similarity1
Metal bindingi323Zinc 2By similarity1
Metal bindingi419Zinc 1By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU05740-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase 3 (EC:3.1.3.1)
Alternative name(s):
Alkaline phosphatase III
Short name:
APase III
Gene namesi
Name:phoB
Synonyms:phoAIII
Ordered Locus Names:BSU05740
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 321 PublicationAdd BLAST32
ChainiPRO_000002401033 – 462Alkaline phosphatase 3Add BLAST430

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP19405.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100003223.

Structurei

3D structure databases

ProteinModelPortaliP19405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105G6Z. Bacteria.
COG1785. LUCA.
HOGENOMiHOG000099116.
InParanoidiP19405.
KOiK01077.
OMAiKNMNSIA.
PhylomeDBiP19405.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKFPKKLLP IAVLSSIAFS SLASGSVPEA SAQEKKKGNQ DEIKNVIVLI
60 70 80 90 100
GDGMGVSYTS AYRYLKDNKK TKVVEPTAFD QYLVGQQTTY PDDPEQNVTD
110 120 130 140 150
SAAAATAMSA GIKTYNNAIA VDNDGSEAKT VLEAAKEKGK ATGLVATSEI
160 170 180 190 200
THATPASFGS HDHSRKNMNS IADDYFDEMV NGKHKIDVLL GGGKSNFDRK
210 220 230 240 250
DRNLIKEFKK AGYSYVDDRK DMLKNKDSQV LGLFADGGLP KKIDRTKDIP
260 270 280 290 300
SLKDMTNTAI KKLNKDKDGF FLMVEGSQID WAGHDNDIVG AMSEMEDFEQ
310 320 330 340 350
AYKAAIDFAK KDKHTLVVAT ADHSTGGYSI GADGIYNWFS EPIKAAKRTP
360 370 380 390 400
DFMAEKIADG ADVEKTLKTY IDQKKLALTK AEIQSVEEAA KSKEVLDIDN
410 420 430 440 450
AIENIFNKRS HTGWTTGGHT GEDVPVYAYG PSSETFAGQI DNTEIAKNVF
460
KALQYNIKIN DK
Length:462
Mass (Da):50,494
Last modified:July 7, 2009 - v4
Checksum:iCB0F8FB855D17231
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti215Y → S (PubMed:1898729).Curated1
Sequence conflicti234 – 235FA → LP (PubMed:1898729).Curated2
Sequence conflicti334G → S in BAA19698 (PubMed:9202461).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88802 Genomic DNA. Translation: BAA19698.1.
AL009126 Genomic DNA. Translation: CAB12393.2.
M33634 Genomic DNA. Translation: AAA22658.1.
PIRiC69676.
RefSeqiNP_388455.2. NC_000964.3.
WP_003234119.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB12393; CAB12393; BSU05740.
GeneIDi938004.
KEGGibsu:BSU05740.
PATRICi18972762. VBIBacSub10457_0602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88802 Genomic DNA. Translation: BAA19698.1.
AL009126 Genomic DNA. Translation: CAB12393.2.
M33634 Genomic DNA. Translation: AAA22658.1.
PIRiC69676.
RefSeqiNP_388455.2. NC_000964.3.
WP_003234119.1. NC_000964.3.

3D structure databases

ProteinModelPortaliP19405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100003223.

Proteomic databases

PaxDbiP19405.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12393; CAB12393; BSU05740.
GeneIDi938004.
KEGGibsu:BSU05740.
PATRICi18972762. VBIBacSub10457_0602.

Phylogenomic databases

eggNOGiENOG4105G6Z. Bacteria.
COG1785. LUCA.
HOGENOMiHOG000099116.
InParanoidiP19405.
KOiK01077.
OMAiKNMNSIA.
PhylomeDBiP19405.

Enzyme and pathway databases

BioCyciBSUB:BSU05740-MONOMER.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPB3_BACSU
AccessioniPrimary (citable) accession number: P19405
Secondary accession number(s): O05498
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: July 7, 2009
Last modified: November 30, 2016
This is version 126 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.