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P19387

- RPB3_HUMAN

UniProt

P19387 - RPB3_HUMAN

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Protein

DNA-directed RNA polymerase II subunit RPB3

Gene
POLR2C, A-152E5.7
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft By similarity.1 Publication

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. 7-methylguanosine mRNA capping Source: Reactome
  2. DNA repair Source: Reactome
  3. gene expression Source: Reactome
  4. mRNA splicing, via spliceosome Source: Reactome
  5. nucleotide-excision repair Source: Reactome
  6. positive regulation of viral transcription Source: Reactome
  7. RNA splicing Source: Reactome
  8. transcription-coupled nucleotide-excision repair Source: Reactome
  9. transcription elongation from RNA polymerase II promoter Source: Reactome
  10. transcription from RNA polymerase II promoter Source: UniProtKB
  11. transcription initiation from RNA polymerase II promoter Source: Reactome
  12. viral process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

ReactomeiREACT_12417. MicroRNA (miRNA) biogenesis.
REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
REACT_1470. mRNA Capping.
REACT_1628. Transcription-coupled NER (TC-NER).
REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1753. mRNA Splicing - Minor Pathway.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
REACT_2222. Dual incision reaction in TC-NER.
REACT_467. mRNA Splicing - Major Pathway.
REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
REACT_6233. Transcription of the HIV genome.
REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_6244. Pausing and recovery of HIV elongation.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6259. HIV elongation arrest and recovery.
REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
REACT_6319. Formation of the HIV-1 Early Elongation Complex.
REACT_6332. HIV Transcription Initiation.
REACT_6344. Tat-mediated HIV elongation arrest and recovery.
REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_833. RNA Polymerase II Transcription Elongation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_846. Formation of the Early Elongation Complex.
REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB3
Short name:
RNA polymerase II subunit 3
Short name:
RNA polymerase II subunit B3
Alternative name(s):
DNA-directed RNA polymerase II 33 kDa polypeptide
Short name:
RPB33
DNA-directed RNA polymerase II subunit C
RPB31
Gene namesi
Name:POLR2C
ORF Names:A-152E5.7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:9189. POLR2C.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. DNA-directed RNA polymerase II, core complex Source: UniProtKB
  3. microtubule cytoskeleton Source: HPA
  4. nucleoplasm Source: Reactome
  5. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33509.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 275274DNA-directed RNA polymerase II subunit RPB3PRO_0000132743Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei124 – 1241Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP19387.
PaxDbiP19387.
PeptideAtlasiP19387.
PRIDEiP19387.

PTM databases

PhosphoSiteiP19387.

Expressioni

Gene expression databases

ArrayExpressiP19387.
BgeeiP19387.
CleanExiHS_POLR2C.
GenevestigatoriP19387.

Organism-specific databases

HPAiHPA040919.
HPA041826.

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB11/POLR2J and RPB3/POLR2C subunits interact with each other.1 Publication

Protein-protein interaction databases

BioGridi111428. 91 interactions.
DIPiDIP-32911N.
IntActiP19387. 19 interactions.
MINTiMINT-270053.
STRINGi9606.ENSP00000219252.

Structurei

3D structure databases

ProteinModelPortaliP19387.
SMRiP19387. Positions 7-268.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi88 – 9710Cys-rich

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0202.
HOVERGENiHBG000897.
InParanoidiP19387.
KOiK03011.
OMAiPINTEEF.
OrthoDBiEOG70S761.
PhylomeDBiP19387.
TreeFamiTF103038.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
InterProiIPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR009025. RBP11-like_dimer.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
PROSITEiPS00446. RNA_POL_D_30KD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19387-1 [UniParc]FASTAAdd to Basket

« Hide

MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV    50
QIDANSSVLH DEFIAHRLGL IPLISDDIVD KLQYSRDCTC EEFCPECSVE 100
FTLDVRCNED QTRHVTSRDL ISNSPRVIPV TSRNRDNDPN DYVEQDDILI 150
VKLRKGQELR LRAYAKKGFG KEHAKWNPTA GVAFEYDPDN ALRHTVYPKP 200
EEWPKSEYSE LDEDESQAPY DPNGKPERFY YNVESCGSLR PETIVLSALS 250
GLKKKLSDLQ TQLSHEIQSD VLTIN 275
Length:275
Mass (Da):31,441
Last modified:December 15, 1998 - v2
Checksum:iEF663BE096046A4B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1941H → T1 Publication
Sequence conflicti194 – 1941H → T1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05448 mRNA. Translation: AAA36586.1.
AF008443 mRNA. Translation: AAC14355.1.
AJ224143 mRNA. Translation: CAA11842.1.
AJ224144 mRNA. Translation: CAA11843.1.
AC004382 Genomic DNA. Translation: AAC24309.1.
BC000409 mRNA. Translation: AAH00409.1.
BC003159 mRNA. Translation: AAH03159.1.
BC028157 mRNA. Translation: AAH28157.1.
CCDSiCCDS10782.1.
PIRiA36264.
RefSeqiNP_116558.1. NM_032940.2.
UniGeneiHs.79402.

Genome annotation databases

EnsembliENST00000219252; ENSP00000219252; ENSG00000102978.
GeneIDi5432.
KEGGihsa:5432.
UCSCiuc002elt.1. human.

Polymorphism databases

DMDMi3915850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05448 mRNA. Translation: AAA36586.1 .
AF008443 mRNA. Translation: AAC14355.1 .
AJ224143 mRNA. Translation: CAA11842.1 .
AJ224144 mRNA. Translation: CAA11843.1 .
AC004382 Genomic DNA. Translation: AAC24309.1 .
BC000409 mRNA. Translation: AAH00409.1 .
BC003159 mRNA. Translation: AAH03159.1 .
BC028157 mRNA. Translation: AAH28157.1 .
CCDSi CCDS10782.1.
PIRi A36264.
RefSeqi NP_116558.1. NM_032940.2.
UniGenei Hs.79402.

3D structure databases

ProteinModelPortali P19387.
SMRi P19387. Positions 7-268.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111428. 91 interactions.
DIPi DIP-32911N.
IntActi P19387. 19 interactions.
MINTi MINT-270053.
STRINGi 9606.ENSP00000219252.

PTM databases

PhosphoSitei P19387.

Polymorphism databases

DMDMi 3915850.

Proteomic databases

MaxQBi P19387.
PaxDbi P19387.
PeptideAtlasi P19387.
PRIDEi P19387.

Protocols and materials databases

DNASUi 5432.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000219252 ; ENSP00000219252 ; ENSG00000102978 .
GeneIDi 5432.
KEGGi hsa:5432.
UCSCi uc002elt.1. human.

Organism-specific databases

CTDi 5432.
GeneCardsi GC16P057496.
HGNCi HGNC:9189. POLR2C.
HPAi HPA040919.
HPA041826.
MIMi 180663. gene.
neXtProti NX_P19387.
PharmGKBi PA33509.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0202.
HOVERGENi HBG000897.
InParanoidi P19387.
KOi K03011.
OMAi PINTEEF.
OrthoDBi EOG70S761.
PhylomeDBi P19387.
TreeFami TF103038.

Enzyme and pathway databases

Reactomei REACT_12417. MicroRNA (miRNA) biogenesis.
REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
REACT_1470. mRNA Capping.
REACT_1628. Transcription-coupled NER (TC-NER).
REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_1753. mRNA Splicing - Minor Pathway.
REACT_1851. RNA Polymerase II Transcription Initiation.
REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_2089. RNA Polymerase II Promoter Escape.
REACT_22107. RNA Polymerase II Pre-transcription Events.
REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
REACT_2222. Dual incision reaction in TC-NER.
REACT_467. mRNA Splicing - Major Pathway.
REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
REACT_6233. Transcription of the HIV genome.
REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_6244. Pausing and recovery of HIV elongation.
REACT_6253. RNA Polymerase II HIV Promoter Escape.
REACT_6259. HIV elongation arrest and recovery.
REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
REACT_6319. Formation of the HIV-1 Early Elongation Complex.
REACT_6332. HIV Transcription Initiation.
REACT_6344. Tat-mediated HIV elongation arrest and recovery.
REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_833. RNA Polymerase II Transcription Elongation.
REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_846. Formation of the Early Elongation Complex.
REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

GeneWikii POLR2C.
GenomeRNAii 5432.
NextBioi 21017.
PROi P19387.
SOURCEi Search...

Gene expression databases

ArrayExpressi P19387.
Bgeei P19387.
CleanExi HS_POLR2C.
Genevestigatori P19387.

Family and domain databases

Gene3Di 2.170.120.12. 1 hit.
InterProi IPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR009025. RBP11-like_dimer.
[Graphical view ]
Pfami PF01000. RNA_pol_A_bac. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view ]
SMARTi SM00662. RPOLD. 1 hit.
[Graphical view ]
SUPFAMi SSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
PROSITEi PS00446. RNA_POL_D_30KD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The amino acid sequence of the human RNA polymerase II 33-kDa subunit hRPB 33 is highly conserved among eukaryotes."
    Pati U.K., Weissman S.M.
    J. Biol. Chem. 265:8400-8403(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and characterization of the human RNA polymerase I subunit hRPA40."
    Dammann R., Pfeifer G.P.
    Biochim. Biophys. Acta 1396:153-157(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Bruno T., di Padova M., de Angelis R., Iacobini C., Lovari S., Passananti C., Fanciulli M.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain, Kidney and Muscle.
  6. Bienvenut W.V., Waridel P., Quadroni M.
    Submitted (MAR-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-35; 68-86; 134-152; 176-193 AND 256-275, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryonic kidney.
  7. "Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes."
    Kershnar E., Wu S.-Y., Chiang C.-M.
    J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE RNA POLYMERASE II CORE-COMPLEX, SUBCELLULAR LOCATION.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRPB3_HUMAN
AccessioniPrimary (citable) accession number: P19387
Secondary accession number(s): O15161
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 15, 1998
Last modified: September 3, 2014
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi