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Protein

DNA-directed RNA polymerase II subunit RPB3

Gene

POLR2C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciZFISH:HS02437-MONOMER.
ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-112387. Elongation arrest and recovery.
R-HSA-113418. Formation of the Early Elongation Complex.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167158. Formation of the HIV-1 Early Elongation Complex.
R-HSA-167160. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167242. Abortive elongation of HIV-1 transcript in the absence of Tat.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-203927. MicroRNA (miRNA) biogenesis.
R-HSA-452723. Transcriptional regulation of pluripotent stem cells.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-HSA-6803529. FGFR2 alternative splicing.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-72086. mRNA Capping.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-HSA-77075. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-8851708. Signaling by FGFR2 IIIa TM.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB3Curated
Short name:
RNA polymerase II subunit 3
Short name:
RNA polymerase II subunit B3
Alternative name(s):
DNA-directed RNA polymerase II 33 kDa polypeptide
Short name:
RPB33
DNA-directed RNA polymerase II subunit C
RPB31By similarity
Gene namesi
Name:POLR2CImported
ORF Names:A-152E5.7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:9189. POLR2C.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasm Source: HPA
  • DNA-directed RNA polymerase II, core complex Source: UniProtKB
  • microtubule cytoskeleton Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5432.
OpenTargetsiENSG00000102978.
PharmGKBiPA33509.

Polymorphism and mutation databases

BioMutaiPOLR2C.
DMDMi3915850.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001327432 – 275DNA-directed RNA polymerase II subunit RPB3Add BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei124PhosphoserineCombined sources1
Modified residuei257PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP19387.
PaxDbiP19387.
PeptideAtlasiP19387.
PRIDEiP19387.

PTM databases

iPTMnetiP19387.
PhosphoSitePlusiP19387.

Expressioni

Gene expression databases

BgeeiENSG00000102978.
CleanExiHS_POLR2C.
ExpressionAtlasiP19387. baseline and differential.
GenevisibleiP19387. HS.

Organism-specific databases

HPAiHPA040919.
HPA041826.

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB11/POLR2J and RPB3/POLR2C subunits interact with each other.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ATF4P188486EBI-394729,EBI-492498

Protein-protein interaction databases

BioGridi111428. 107 interactors.
DIPiDIP-32911N.
IntActiP19387. 37 interactors.
MINTiMINT-270053.
STRINGi9606.ENSP00000219252.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IY6electron microscopy7.20C1-275[»]
5IY7electron microscopy8.60C1-275[»]
5IY8electron microscopy7.90C1-275[»]
5IY9electron microscopy6.30C1-275[»]
5IYAelectron microscopy5.40C1-275[»]
5IYBelectron microscopy3.90C1-275[»]
5IYCelectron microscopy3.90C1-275[»]
5IYDelectron microscopy3.90C1-275[»]
ProteinModelPortaliP19387.
SMRiP19387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi88 – 97Cys-rich10

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1522. Eukaryota.
COG0202. LUCA.
GeneTreeiENSGT00640000091532.
HOVERGENiHBG000897.
InParanoidiP19387.
KOiK03011.
OMAiDFCQECS.
OrthoDBiEOG091G0EGB.
PhylomeDBiP19387.
TreeFamiTF103038.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
InterProiIPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR009025. RBP11-like_dimer.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
PROSITEiPS00446. RNA_POL_D_30KD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19387-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV
60 70 80 90 100
QIDANSSVLH DEFIAHRLGL IPLISDDIVD KLQYSRDCTC EEFCPECSVE
110 120 130 140 150
FTLDVRCNED QTRHVTSRDL ISNSPRVIPV TSRNRDNDPN DYVEQDDILI
160 170 180 190 200
VKLRKGQELR LRAYAKKGFG KEHAKWNPTA GVAFEYDPDN ALRHTVYPKP
210 220 230 240 250
EEWPKSEYSE LDEDESQAPY DPNGKPERFY YNVESCGSLR PETIVLSALS
260 270
GLKKKLSDLQ TQLSHEIQSD VLTIN
Length:275
Mass (Da):31,441
Last modified:December 15, 1998 - v2
Checksum:iEF663BE096046A4B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194H → T (PubMed:2187864).Curated1
Sequence conflicti194H → T (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05448 mRNA. Translation: AAA36586.1.
AF008443 mRNA. Translation: AAC14355.1.
AJ224143 mRNA. Translation: CAA11842.1.
AJ224144 mRNA. Translation: CAA11843.1.
AC004382 Genomic DNA. Translation: AAC24309.1.
BC000409 mRNA. Translation: AAH00409.1.
BC003159 mRNA. Translation: AAH03159.1.
BC028157 mRNA. Translation: AAH28157.1.
CCDSiCCDS10782.1.
PIRiA36264.
RefSeqiNP_116558.1. NM_032940.2.
UniGeneiHs.79402.

Genome annotation databases

EnsembliENST00000219252; ENSP00000219252; ENSG00000102978.
GeneIDi5432.
KEGGihsa:5432.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05448 mRNA. Translation: AAA36586.1.
AF008443 mRNA. Translation: AAC14355.1.
AJ224143 mRNA. Translation: CAA11842.1.
AJ224144 mRNA. Translation: CAA11843.1.
AC004382 Genomic DNA. Translation: AAC24309.1.
BC000409 mRNA. Translation: AAH00409.1.
BC003159 mRNA. Translation: AAH03159.1.
BC028157 mRNA. Translation: AAH28157.1.
CCDSiCCDS10782.1.
PIRiA36264.
RefSeqiNP_116558.1. NM_032940.2.
UniGeneiHs.79402.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IY6electron microscopy7.20C1-275[»]
5IY7electron microscopy8.60C1-275[»]
5IY8electron microscopy7.90C1-275[»]
5IY9electron microscopy6.30C1-275[»]
5IYAelectron microscopy5.40C1-275[»]
5IYBelectron microscopy3.90C1-275[»]
5IYCelectron microscopy3.90C1-275[»]
5IYDelectron microscopy3.90C1-275[»]
ProteinModelPortaliP19387.
SMRiP19387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111428. 107 interactors.
DIPiDIP-32911N.
IntActiP19387. 37 interactors.
MINTiMINT-270053.
STRINGi9606.ENSP00000219252.

PTM databases

iPTMnetiP19387.
PhosphoSitePlusiP19387.

Polymorphism and mutation databases

BioMutaiPOLR2C.
DMDMi3915850.

Proteomic databases

EPDiP19387.
PaxDbiP19387.
PeptideAtlasiP19387.
PRIDEiP19387.

Protocols and materials databases

DNASUi5432.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219252; ENSP00000219252; ENSG00000102978.
GeneIDi5432.
KEGGihsa:5432.

Organism-specific databases

CTDi5432.
DisGeNETi5432.
GeneCardsiPOLR2C.
HGNCiHGNC:9189. POLR2C.
HPAiHPA040919.
HPA041826.
MIMi180663. gene.
neXtProtiNX_P19387.
OpenTargetsiENSG00000102978.
PharmGKBiPA33509.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1522. Eukaryota.
COG0202. LUCA.
GeneTreeiENSGT00640000091532.
HOVERGENiHBG000897.
InParanoidiP19387.
KOiK03011.
OMAiDFCQECS.
OrthoDBiEOG091G0EGB.
PhylomeDBiP19387.
TreeFamiTF103038.

Enzyme and pathway databases

BioCyciZFISH:HS02437-MONOMER.
ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-112387. Elongation arrest and recovery.
R-HSA-113418. Formation of the Early Elongation Complex.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167158. Formation of the HIV-1 Early Elongation Complex.
R-HSA-167160. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167242. Abortive elongation of HIV-1 transcript in the absence of Tat.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-203927. MicroRNA (miRNA) biogenesis.
R-HSA-452723. Transcriptional regulation of pluripotent stem cells.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-HSA-6803529. FGFR2 alternative splicing.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-72086. mRNA Capping.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-HSA-77075. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-8851708. Signaling by FGFR2 IIIa TM.

Miscellaneous databases

GeneWikiiPOLR2C.
GenomeRNAii5432.
PROiP19387.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102978.
CleanExiHS_POLR2C.
ExpressionAtlasiP19387. baseline and differential.
GenevisibleiP19387. HS.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
InterProiIPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR009025. RBP11-like_dimer.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
PROSITEiPS00446. RNA_POL_D_30KD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPB3_HUMAN
AccessioniPrimary (citable) accession number: P19387
Secondary accession number(s): O15161
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 15, 1998
Last modified: November 30, 2016
This is version 179 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.