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P19387

- RPB3_HUMAN

UniProt

P19387 - RPB3_HUMAN

Protein

DNA-directed RNA polymerase II subunit RPB3

Gene

POLR2C

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 155 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft By similarity.By similarity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. 7-methylguanosine mRNA capping Source: Reactome
    2. DNA repair Source: Reactome
    3. gene expression Source: Reactome
    4. mRNA splicing, via spliceosome Source: Reactome
    5. nucleotide-excision repair Source: Reactome
    6. positive regulation of viral transcription Source: Reactome
    7. RNA splicing Source: Reactome
    8. transcription-coupled nucleotide-excision repair Source: Reactome
    9. transcription elongation from RNA polymerase II promoter Source: Reactome
    10. transcription from RNA polymerase II promoter Source: UniProtKB
    11. transcription initiation from RNA polymerase II promoter Source: Reactome
    12. viral process Source: Reactome

    Keywords - Biological processi

    Transcription

    Enzyme and pathway databases

    ReactomeiREACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase II subunit RPB3
    Short name:
    RNA polymerase II subunit 3
    Short name:
    RNA polymerase II subunit B3
    Alternative name(s):
    DNA-directed RNA polymerase II 33 kDa polypeptide
    Short name:
    RPB33
    DNA-directed RNA polymerase II subunit C
    RPB31
    Gene namesi
    Name:POLR2C
    ORF Names:A-152E5.7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:9189. POLR2C.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. DNA-directed RNA polymerase II, core complex Source: UniProtKB
    3. microtubule cytoskeleton Source: HPA
    4. nucleoplasm Source: Reactome
    5. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33509.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 275274DNA-directed RNA polymerase II subunit RPB3PRO_0000132743Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei124 – 1241Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP19387.
    PaxDbiP19387.
    PeptideAtlasiP19387.
    PRIDEiP19387.

    PTM databases

    PhosphoSiteiP19387.

    Expressioni

    Gene expression databases

    ArrayExpressiP19387.
    BgeeiP19387.
    CleanExiHS_POLR2C.
    GenevestigatoriP19387.

    Organism-specific databases

    HPAiHPA040919.
    HPA041826.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB11/POLR2J and RPB3/POLR2C subunits interact with each other.1 Publication

    Protein-protein interaction databases

    BioGridi111428. 91 interactions.
    DIPiDIP-32911N.
    IntActiP19387. 19 interactions.
    MINTiMINT-270053.
    STRINGi9606.ENSP00000219252.

    Structurei

    3D structure databases

    ProteinModelPortaliP19387.
    SMRiP19387. Positions 7-268.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi88 – 9710Cys-rich

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0202.
    HOVERGENiHBG000897.
    InParanoidiP19387.
    KOiK03011.
    OMAiPINTEEF.
    OrthoDBiEOG70S761.
    PhylomeDBiP19387.
    TreeFamiTF103038.

    Family and domain databases

    Gene3Di2.170.120.12. 1 hit.
    InterProiIPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
    IPR011262. DNA-dir_RNA_pol_insert.
    IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
    IPR009025. RBP11-like_dimer.
    [Graphical view]
    PfamiPF01000. RNA_pol_A_bac. 1 hit.
    PF01193. RNA_pol_L. 1 hit.
    [Graphical view]
    SMARTiSM00662. RPOLD. 1 hit.
    [Graphical view]
    SUPFAMiSSF55257. SSF55257. 2 hits.
    SSF56553. SSF56553. 1 hit.
    PROSITEiPS00446. RNA_POL_D_30KD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19387-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV    50
    QIDANSSVLH DEFIAHRLGL IPLISDDIVD KLQYSRDCTC EEFCPECSVE 100
    FTLDVRCNED QTRHVTSRDL ISNSPRVIPV TSRNRDNDPN DYVEQDDILI 150
    VKLRKGQELR LRAYAKKGFG KEHAKWNPTA GVAFEYDPDN ALRHTVYPKP 200
    EEWPKSEYSE LDEDESQAPY DPNGKPERFY YNVESCGSLR PETIVLSALS 250
    GLKKKLSDLQ TQLSHEIQSD VLTIN 275
    Length:275
    Mass (Da):31,441
    Last modified:December 15, 1998 - v2
    Checksum:iEF663BE096046A4B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti194 – 1941H → T(PubMed:2187864)Curated
    Sequence conflicti194 – 1941H → T1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05448 mRNA. Translation: AAA36586.1.
    AF008443 mRNA. Translation: AAC14355.1.
    AJ224143 mRNA. Translation: CAA11842.1.
    AJ224144 mRNA. Translation: CAA11843.1.
    AC004382 Genomic DNA. Translation: AAC24309.1.
    BC000409 mRNA. Translation: AAH00409.1.
    BC003159 mRNA. Translation: AAH03159.1.
    BC028157 mRNA. Translation: AAH28157.1.
    CCDSiCCDS10782.1.
    PIRiA36264.
    RefSeqiNP_116558.1. NM_032940.2.
    UniGeneiHs.79402.

    Genome annotation databases

    EnsembliENST00000219252; ENSP00000219252; ENSG00000102978.
    GeneIDi5432.
    KEGGihsa:5432.
    UCSCiuc002elt.1. human.

    Polymorphism databases

    DMDMi3915850.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05448 mRNA. Translation: AAA36586.1 .
    AF008443 mRNA. Translation: AAC14355.1 .
    AJ224143 mRNA. Translation: CAA11842.1 .
    AJ224144 mRNA. Translation: CAA11843.1 .
    AC004382 Genomic DNA. Translation: AAC24309.1 .
    BC000409 mRNA. Translation: AAH00409.1 .
    BC003159 mRNA. Translation: AAH03159.1 .
    BC028157 mRNA. Translation: AAH28157.1 .
    CCDSi CCDS10782.1.
    PIRi A36264.
    RefSeqi NP_116558.1. NM_032940.2.
    UniGenei Hs.79402.

    3D structure databases

    ProteinModelPortali P19387.
    SMRi P19387. Positions 7-268.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111428. 91 interactions.
    DIPi DIP-32911N.
    IntActi P19387. 19 interactions.
    MINTi MINT-270053.
    STRINGi 9606.ENSP00000219252.

    PTM databases

    PhosphoSitei P19387.

    Polymorphism databases

    DMDMi 3915850.

    Proteomic databases

    MaxQBi P19387.
    PaxDbi P19387.
    PeptideAtlasi P19387.
    PRIDEi P19387.

    Protocols and materials databases

    DNASUi 5432.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000219252 ; ENSP00000219252 ; ENSG00000102978 .
    GeneIDi 5432.
    KEGGi hsa:5432.
    UCSCi uc002elt.1. human.

    Organism-specific databases

    CTDi 5432.
    GeneCardsi GC16P057496.
    HGNCi HGNC:9189. POLR2C.
    HPAi HPA040919.
    HPA041826.
    MIMi 180663. gene.
    neXtProti NX_P19387.
    PharmGKBi PA33509.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0202.
    HOVERGENi HBG000897.
    InParanoidi P19387.
    KOi K03011.
    OMAi PINTEEF.
    OrthoDBi EOG70S761.
    PhylomeDBi P19387.
    TreeFami TF103038.

    Enzyme and pathway databases

    Reactomei REACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Miscellaneous databases

    GeneWikii POLR2C.
    GenomeRNAii 5432.
    NextBioi 21017.
    PROi P19387.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P19387.
    Bgeei P19387.
    CleanExi HS_POLR2C.
    Genevestigatori P19387.

    Family and domain databases

    Gene3Di 2.170.120.12. 1 hit.
    InterProi IPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
    IPR011262. DNA-dir_RNA_pol_insert.
    IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
    IPR009025. RBP11-like_dimer.
    [Graphical view ]
    Pfami PF01000. RNA_pol_A_bac. 1 hit.
    PF01193. RNA_pol_L. 1 hit.
    [Graphical view ]
    SMARTi SM00662. RPOLD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55257. SSF55257. 2 hits.
    SSF56553. SSF56553. 1 hit.
    PROSITEi PS00446. RNA_POL_D_30KD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The amino acid sequence of the human RNA polymerase II 33-kDa subunit hRPB 33 is highly conserved among eukaryotes."
      Pati U.K., Weissman S.M.
      J. Biol. Chem. 265:8400-8403(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and characterization of the human RNA polymerase I subunit hRPA40."
      Dammann R., Pfeifer G.P.
      Biochim. Biophys. Acta 1396:153-157(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Bruno T., di Padova M., de Angelis R., Iacobini C., Lovari S., Passananti C., Fanciulli M.
      Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skeletal muscle.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain, Kidney and Muscle.
    6. Bienvenut W.V., Waridel P., Quadroni M.
      Submitted (MAR-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-35; 68-86; 134-152; 176-193 AND 256-275, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryonic kidney.
    7. "Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes."
      Kershnar E., Wu S.-Y., Chiang C.-M.
      J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RNA POLYMERASE II CORE-COMPLEX, SUBCELLULAR LOCATION.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRPB3_HUMAN
    AccessioniPrimary (citable) accession number: P19387
    Secondary accession number(s): O15161
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 155 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3