P19386 (LYS_BPCP9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme
EC=3.2.1.17

Alternative name(s):
CP-9 lysin
Endolysin
Muramidase

Gene names

Name:

CPL9

Organism

Streptococcus phage Cp-9 (Bacteriophage Cp-9)

Taxonomic identifier

10749 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Picovirinae › unassigned Picovirinae › Streptococcus phage Cp-1

Virus host

Streptococcus pneumoniae [TaxID: 1313]

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the separation of the host daughter cells at the end of cell division and participates in the liberation of progeny bacteriophage into the medium. Strictly depends on the presence of choline-containing cell walls for activity.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Domain	The C-terminal domain comprising the repeats is involved in choline binding By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 25 family. Contains 6 cell wall-binding repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Lysozyme		PRO_0000208261
Regions
	Repeat	200 – 219	20	Cell wall-binding 1
	Repeat	220 – 239	20	Cell wall-binding 2
	Repeat	241 – 260	20	Cell wall-binding 3
	Repeat	261 – 280	20	Cell wall-binding 4
	Repeat	281 – 300	20	Cell wall-binding 5
	Repeat	303 – 322	20	Cell wall-binding 6
Sites
	Active site	10	1	By similarity
	Active site	94	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19386 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: A2143F05BDED4EDD	FASTA	339	39,151
	10 20 30 40 50 60 MVKKNDLFID VSSHNGYDIT GILEQMGTTN TIVKISESTT YLNPCLSAQV EQSTPIGFYH 70 80 90 100 110 120 FARFGGDVAE AEREAQFFLD NVPTQVKYLV LDYEDDPSGN AQANTNACLR FMQMIADAGY 130 140 150 160 170 180 TPIYYSYKPF TLDNVDYQQI LAQFPNSLWI AGYGLNDGNA DFEYFPSMDG IRWWQYSSNP 190 200 210 220 230 240 FDKNIVLLDD EEDEKPKTAG TWKQDSKGWW FRRNNGSFPY NKWEKIGGVW YYFDSKGYCL 250 260 270 280 290 300 TSEWLKDNEK WYYLKDNGAM VTGWVLVGSE WYYMDDSGAM VTGWVKYKNN WYYMTNERGN 310 320 330 MVSNEFIKSG KGWYFMNTNG ELADNPSFTK EPDGLITVA

References

[1]	"Modular organization of the lytic enzymes of Streptococcus pneumoniae and its bacteriophages." Garcia P., Garcia J.L., Garcia E., Sanchez-Puelles J.M., Lopez R. Gene 86:81-88(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34780 Genomic DNA. Translation: AAA72845.1.
PIR	MUBPC9. JQ0438.
3D structure databases
ProteinModelPortal	P19386.
SMR	P19386. Positions 2-339.
ModBase	Search...
Protein family/group databases
CAZy	GH25. Glycoside Hydrolase Family 25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR018337. Cell_wall/Cho-bd_repeat. IPR002053. Glyco_hydro_25. IPR008270. Glyco_hydro_25_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR018077. Glyco_hydro_fam25_subgr. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF01473. CW_binding_1. 5 hits. PF01183. Glyco_hydro_25. 1 hit. [Graphical view]
SMART	SM00641. Glyco_25. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS51170. CW. 5 hits. PS00953. GLYCOSYL_HYDROL_F25. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS_BPCP9

Accession

Primary (citable) accession number: P19386

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program