P19385 (LYS_BPCP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme
EC=3.2.1.17

Alternative name(s):
CP-7 lysin
Endolysin
Muramidase

Gene names

Name:

CPL7

Organism

Streptococcus phage Cp-7 (Bacteriophage Cp-7)

Taxonomic identifier

10748 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Picovirinae › unassigned Picovirinae › Streptococcus phage Cp-1

Virus host

Streptococcus pneumoniae [TaxID: 1313]

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the separation of the host daughter cells at the end of cell division and participates in the liberation of progeny bacteriophage into the medium. Degrades cell walls containing either choline or ethanolamine.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Domain	The C-terminal domain could be responsible for the substrate recognition.
Sequence similarities	Belongs to the glycosyl hydrolase 25 family.

Ontologies

Keywords
Domain	Repeat
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Lysozyme		PRO_0000208260
Regions
	Repeat	205 – 242	38	1; truncated
	Repeat	243 – 290	48	2
	Repeat	291 – 338	48	3
	Region	205 – 338	134	3 X 48 AA tandem repeats
Sites
	Active site	10	1	By similarity
	Active site	94	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19385 [UniParc]. Last modified September 5, 2012. Version 2. Checksum: 6B1F431DD068CC52	FASTA	342	38,248
	10 20 30 40 50 60 MVKKNDLFVD VASHQGYDIS GILEEAGTTN TIIKVSESTS YLNPCLSAQV SQSNPIGFYH 70 80 90 100 110 120 FAWFGGNEEE AEAEARYFLD NVPTQVKYLV LDYEDHASAS VQRNTTACLR FMQIIAEAGY 130 140 150 160 170 180 TPIYYSYKPF TLDNVDYQQI LAQFPNSLWI AGYGLNDGTA NFEYFPSMDG IRWWQYSSNP 190 200 210 220 230 240 FDKNIVLLDD EKEDNINNEN TLKSLTTVAN EVIQGLWGNG QERYDSLANA GYDPQAVQDK 250 260 270 280 290 300 VNEILNAREI ADLTTVANEV IQGLWGNGQE RYDSLANAGY DPQAVQDKVN EILNAREIAD 310 320 330 340 LTTVANEVIQ GLWGNGQERY DSLANAGYDP QAVQDKVNEL LS

References

[1]	"Modular organization of the lytic enzymes of Streptococcus pneumoniae and its bacteriophages." Garcia P., Garcia J.L., Garcia E., Sanchez-Puelles J.M., Lopez R. Gene 86:81-88(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Garcia P., Garcia J.L., Garcia E., Sanchez-Puelles J.M., Lopez R. Submitted (MAR-2012) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 63; 114; 230; 278 AND 326.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34779 Genomic DNA. Translation: AAA72844.2.
PIR	MUBPC7. JQ0437.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH25. Glycoside Hydrolase Family 25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR013168. Cpl_7_lyso_C. IPR002053. Glyco_hydro_25. IPR008270. Glyco_hydro_25_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR018077. Glyco_hydro_fam25_subgr. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF08230. Cpl-7. 3 hits. PF01183. Glyco_hydro_25. 1 hit. [Graphical view]
SMART	SM01095. Cpl-7. 3 hits. SM00641. Glyco_25. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00953. GLYCOSYL_HYDROL_F25. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS_BPCP7

Accession

Primary (citable) accession number: P19385

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	September 5, 2012
Last modified:	May 1, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program