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Protein

T-cell surface glycoprotein CD3 delta chain

Gene

Cd3d

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-RNO-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-RNO-202424. Downstream TCR signaling.
R-RNO-202427. Phosphorylation of CD3 and TCR zeta chains.
R-RNO-202430. Translocation of ZAP-70 to Immunological synapse.
R-RNO-202433. Generation of second messenger molecules.
R-RNO-389948. PD-1 signaling.
R-RNO-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-RNO-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 delta chain
Alternative name(s):
T-cell receptor T3 delta chain
CD_antigen: CD3d
Gene namesi
Name:Cd3d
Synonyms:T3d
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2304. Cd3d.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 106ExtracellularSequence analysisAdd BLAST85
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128 – 173CytoplasmicSequence analysisAdd BLAST46

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001649122 – 173T-cell surface glycoprotein CD3 delta chainAdd BLAST152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 73By similarity
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi55N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi74N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei149PhosphotyrosinePROSITE-ProRule annotationBy similarity1
Modified residuei160PhosphotyrosinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP19377.
PRIDEiP19377.

Expressioni

Tissue specificityi

CD3D is mostly present on T-lymphocytes with its TCR-CD3 partners. Present also in fetal NK-cells.By similarity

Gene expression databases

BgeeiENSRNOG00000015994.
GenevisibleiP19377. RN.

Interactioni

Subunit structurei

The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with coreceptors CD4 and CD8.By similarity

GO - Molecular functioni

Protein-protein interaction databases

ELMiP19377.
STRINGi10116.ENSRNOP00000021489.

Structurei

3D structure databases

ProteinModelPortaliP19377.
SMRiP19377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 166ITAMPROSITE-ProRule annotationAdd BLAST29

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWAX. Eukaryota.
ENOG410Y2TD. LUCA.
GeneTreeiENSGT00510000046930.
HOGENOMiHOG000015287.
HOVERGENiHBG005278.
InParanoidiP19377.
KOiK06450.
OMAiMCQSCVE.
OrthoDBiEOG091G0U7X.
PhylomeDBiP19377.
TreeFamiTF335892.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015484. CD3_esu/gsu/dsu.
IPR015485. CD3D.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR032052. Ig_4.
IPR003110. Phos_immunorcpt_sig_ITAM.
PANTHERiPTHR10570. PTHR10570. 1 hit.
PTHR10570:SF5. PTHR10570:SF5. 1 hit.
PfamiView protein in Pfam
PF16680. Ig_4. 1 hit.
PF02189. ITAM. 1 hit.
SMARTiView protein in SMART
SM00077. ITAM. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiView protein in PROSITE
PS51055. ITAM_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHYGILVSL LLATVLPQGS PFKIEVVEYE DKVFVNCNTS IRHLDGSVER
60 70 80 90 100
WLTKNKSLIL GKGILDPRGM YMCNGTEELA KEVSTVQVYY RMCQNCVELD
110 120 130 140 150
SATLAGVIIT DLIATLLLAL GVYCFAGHET GRLSGAVDTQ VLLKNEQLYQ
160 170
PLRDRDDAQY SRLGGNWPRN KRS
Length:173
Mass (Da):19,356
Last modified:November 1, 1990 - v1
Checksum:iC462DFF34E7FDB60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53430 mRNA. Translation: CAA37521.1.
PIRiS10975.
RefSeqiNP_037301.1. NM_013169.1.
UniGeneiRn.10329.

Genome annotation databases

EnsembliENSRNOT00000021488; ENSRNOP00000021489; ENSRNOG00000015994.
GeneIDi25710.
KEGGirno:25710.
UCSCiRGD:2304. rat.

Similar proteinsi

Entry informationi

Entry nameiCD3D_RAT
AccessioniPrimary (citable) accession number: P19377
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: October 25, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome