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Protein

Thymidylate synthase

Gene

thyA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei153 – 1531UniRule annotation

GO - Molecular functioni

  1. thymidylate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. dTMP biosynthetic process Source: UniProtKB-HAMAP
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciLLAC272623:GHSH-1638-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:LL1541
ORF Names:L182559
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
ProteomesiUP000002196 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Thymidylate synthasePRO_0000140969Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi272623.L182559.

Structurei

3D structure databases

ProteinModelPortaliP19368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257898.
KOiK00560.
OMAiANPWNRR.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYADKIFKQ NIQNILDNGV FSENARPKYK DGQTANSKYV TGSFVTYDLQ
60 70 80 90 100
KGEFPITTLR PIPIKSAIKE LMWIYQDQTS ELAILEEKYG VKYWGEWGIG
110 120 130 140 150
DGTIGQRYGA TVKKYNIIGK LLDGLAKNPW NRRNIINLWQ YEDFEETEGL
160 170 180 190 200
LPCAFQTMFD VRREQDGQIY LDATLIQRSN DMLVAHHINA MQYVALQMMI
210 220 230 240 250
AKHFSWKVGK FFYFVNNLHI YDNQFEQANE LVKRTASDKE PRLVLNVPDG
260 270
TNFFDIKPED FELVDYEPVK PQLKFDLAI
Length:279
Mass (Da):32,499
Last modified:April 27, 2001 - v2
Checksum:iCE0B97935D8DA1F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 72KI → QV in AAA25221 (PubMed:2117882).Curated
Sequence conflicti34 – 341T → M in AAA25221 (PubMed:2117882).Curated
Sequence conflicti83 – 842AI → SV in AAA25221 (PubMed:2117882).Curated
Sequence conflicti123 – 1231D → E in AAA25221 (PubMed:2117882).Curated
Sequence conflicti165 – 1651Q → K in AAA25221 (PubMed:2117882).Curated
Sequence conflicti232 – 2321V → M in AAA25221 (PubMed:2117882).Curated
Sequence conflicti238 – 2381D → E in AAA25221 (PubMed:2117882).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33770 Genomic DNA. Translation: AAA25221.1.
AE005176 Genomic DNA. Translation: AAK05639.1.
PIRiA43797.
E86817.
RefSeqiNP_267697.1. NC_002662.1.

Genome annotation databases

EnsemblBacteriaiAAK05639; AAK05639; L182559.
GeneIDi1115198.
KEGGilla:L182559.
PATRICi22295490. VBILacLac136773_1652.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33770 Genomic DNA. Translation: AAA25221.1.
AE005176 Genomic DNA. Translation: AAK05639.1.
PIRiA43797.
E86817.
RefSeqiNP_267697.1. NC_002662.1.

3D structure databases

ProteinModelPortaliP19368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272623.L182559.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK05639; AAK05639; L182559.
GeneIDi1115198.
KEGGilla:L182559.
PATRICi22295490. VBILacLac136773_1652.

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257898.
KOiK00560.
OMAiANPWNRR.
OrthoDBiEOG6K6V53.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciLLAC272623:GHSH-1638-MONOMER.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the thymidylate synthase gene from Lactococcus lactis subsp. lactis."
    Ross P., O'Gara F., Condon S.
    Appl. Environ. Microbiol. 56:2156-2163(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 712.
  2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
    Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
    Genome Res. 11:731-753(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IL1403.

Entry informationi

Entry nameiTYSY_LACLA
AccessioniPrimary (citable) accession number: P19368
Secondary accession number(s): Q9CFD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: April 27, 2001
Last modified: January 7, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.