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P19368

- TYSY_LACLA

UniProt

P19368 - TYSY_LACLA

Protein

Thymidylate synthase

Gene

thyA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (27 Apr 2001)
      Previous versions | rss
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    • Comment

    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei153 – 1531UniRule annotation

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dTMP biosynthetic process Source: UniProtKB-HAMAP
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciLLAC272623:GHSH-1638-MONOMER.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TSUniRule annotation
    Short name:
    TSaseUniRule annotation
    Gene namesi
    Name:thyAUniRule annotation
    Ordered Locus Names:LL1541
    ORF Names:L182559
    OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
    Taxonomic identifieri272623 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
    ProteomesiUP000002196: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 279279Thymidylate synthasePRO_0000140969Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi272623.L182559.

    Structurei

    3D structure databases

    ProteinModelPortaliP19368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0207.
    HOGENOMiHOG000257898.
    KOiK00560.
    OMAiTIGHAYG.
    OrthoDBiEOG6K6V53.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19368-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTYADKIFKQ NIQNILDNGV FSENARPKYK DGQTANSKYV TGSFVTYDLQ    50
    KGEFPITTLR PIPIKSAIKE LMWIYQDQTS ELAILEEKYG VKYWGEWGIG 100
    DGTIGQRYGA TVKKYNIIGK LLDGLAKNPW NRRNIINLWQ YEDFEETEGL 150
    LPCAFQTMFD VRREQDGQIY LDATLIQRSN DMLVAHHINA MQYVALQMMI 200
    AKHFSWKVGK FFYFVNNLHI YDNQFEQANE LVKRTASDKE PRLVLNVPDG 250
    TNFFDIKPED FELVDYEPVK PQLKFDLAI 279
    Length:279
    Mass (Da):32,499
    Last modified:April 27, 2001 - v2
    Checksum:iCE0B97935D8DA1F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 72KI → QV in AAA25221. (PubMed:2117882)Curated
    Sequence conflicti34 – 341T → M in AAA25221. (PubMed:2117882)Curated
    Sequence conflicti83 – 842AI → SV in AAA25221. (PubMed:2117882)Curated
    Sequence conflicti123 – 1231D → E in AAA25221. (PubMed:2117882)Curated
    Sequence conflicti165 – 1651Q → K in AAA25221. (PubMed:2117882)Curated
    Sequence conflicti232 – 2321V → M in AAA25221. (PubMed:2117882)Curated
    Sequence conflicti238 – 2381D → E in AAA25221. (PubMed:2117882)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33770 Genomic DNA. Translation: AAA25221.1.
    AE005176 Genomic DNA. Translation: AAK05639.1.
    PIRiA43797.
    E86817.
    RefSeqiNP_267697.1. NC_002662.1.

    Genome annotation databases

    EnsemblBacteriaiAAK05639; AAK05639; L182559.
    GeneIDi1115198.
    KEGGilla:L182559.
    PATRICi22295490. VBILacLac136773_1652.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33770 Genomic DNA. Translation: AAA25221.1 .
    AE005176 Genomic DNA. Translation: AAK05639.1 .
    PIRi A43797.
    E86817.
    RefSeqi NP_267697.1. NC_002662.1.

    3D structure databases

    ProteinModelPortali P19368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272623.L182559.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK05639 ; AAK05639 ; L182559 .
    GeneIDi 1115198.
    KEGGi lla:L182559.
    PATRICi 22295490. VBILacLac136773_1652.

    Phylogenomic databases

    eggNOGi COG0207.
    HOGENOMi HOG000257898.
    KOi K00560.
    OMAi TIGHAYG.
    OrthoDBi EOG6K6V53.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    BioCyci LLAC272623:GHSH-1638-MONOMER.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the thymidylate synthase gene from Lactococcus lactis subsp. lactis."
      Ross P., O'Gara F., Condon S.
      Appl. Environ. Microbiol. 56:2156-2163(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 712.
    2. "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
      Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
      Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IL1403.

    Entry informationi

    Entry nameiTYSY_LACLA
    AccessioniPrimary (citable) accession number: P19368
    Secondary accession number(s): Q9CFD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3