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P19368 (TYSY_LACLA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:LL1541
ORF Names:L182559
OrganismLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) [Reference proteome] [HAMAP]
Taxonomic identifier272623 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus ›

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140969

Sites

Active site1531 By similarity

Experimental info

Sequence conflict6 – 72KI → QV in AAA25221. Ref.1
Sequence conflict341T → M in AAA25221. Ref.1
Sequence conflict83 – 842AI → SV in AAA25221. Ref.1
Sequence conflict1231D → E in AAA25221. Ref.1
Sequence conflict1651Q → K in AAA25221. Ref.1
Sequence conflict2321V → M in AAA25221. Ref.1
Sequence conflict2381D → E in AAA25221. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P19368 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: CE0B97935D8DA1F8

FASTA27932,499
        10         20         30         40         50         60 
MTYADKIFKQ NIQNILDNGV FSENARPKYK DGQTANSKYV TGSFVTYDLQ KGEFPITTLR 

        70         80         90        100        110        120 
PIPIKSAIKE LMWIYQDQTS ELAILEEKYG VKYWGEWGIG DGTIGQRYGA TVKKYNIIGK 

       130        140        150        160        170        180 
LLDGLAKNPW NRRNIINLWQ YEDFEETEGL LPCAFQTMFD VRREQDGQIY LDATLIQRSN 

       190        200        210        220        230        240 
DMLVAHHINA MQYVALQMMI AKHFSWKVGK FFYFVNNLHI YDNQFEQANE LVKRTASDKE 

       250        260        270 
PRLVLNVPDG TNFFDIKPED FELVDYEPVK PQLKFDLAI

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the thymidylate synthase gene from Lactococcus lactis subsp. lactis."
Ross P., O'Gara F., Condon S.
Appl. Environ. Microbiol. 56:2156-2163(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 712.
[2]"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.
Genome Res. 11:731-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IL1403.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M33770 Genomic DNA. Translation: AAA25221.1.
AE005176 Genomic DNA. Translation: AAK05639.1.
PIRA43797.
E86817.
RefSeqNP_267697.1. NC_002662.1.

3D structure databases

ProteinModelPortalP19368.
ModBaseSearch...

Protein-protein interaction databases

STRING272623.L182559.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK05639; AAK05639; L182559.
GeneID1115198.
KEGGlla:L182559.
PATRIC22295490. VBILacLac136773_1652.

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257898.
KOK00560.
OMAANPWNRR.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycLLAC272623:GHSH-1678-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_LACLA
AccessionPrimary (citable) accession number: P19368
Secondary accession number(s): Q9CFD8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: April 27, 2001
Last modified: May 1, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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