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Protein

Achaete-scute homolog 1

Gene

Ascl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional regulator. May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Involved in the initiation of neuronal differentiation. Mediates transcription activation by binding to the E box (5'-CANNTG-3') (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Achaete-scute homolog 1
Gene namesi
Name:Ascl1
Synonyms:Ash1, Mash-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi71010. Ascl1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233Achaete-scute homolog 1PRO_0000127128Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei153 – 1531N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP19359.

Expressioni

Tissue specificityi

Developing CNS and PNS at embryonic and post-natal stages.1 Publication

Developmental stagei

It is first observed after neurulation, in 10.5 day old (E10.5) rat embryos, and is restricted to subsets of neuroepithelial cells in the spinal cord and the brain, between E10.5 and E13.5. In the periphery, its expression is restricted to some lineages of neural crest-derived cells, namely in sympathetic and enteric neural precursors. In the PNS its expression is extinguished at or before differentiation.1 Publication

Gene expression databases

GenevisibleiP19359. RN.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3.

GO - Molecular functioni

  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP19359. 1 interaction.
MINTiMINT-3376271.
STRINGi10116.ENSRNOP00000005674.

Structurei

3D structure databases

ProteinModelPortaliP19359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini115 – 16753bHLHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi30 – 4415Poly-AlaAdd
BLAST
Compositional biasi48 – 547Poly-Gln

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00530000063321.
HOGENOMiHOG000013056.
HOVERGENiHBG050590.
InParanoidiP19359.
KOiK09067.
OMAiRCKRRLN.
OrthoDBiEOG7RFTMH.
PhylomeDBiP19359.
TreeFamiTF322889.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR015660. Achaete-scute-rel.
IPR011598. bHLH_dom.
[Graphical view]
PANTHERiPTHR13935. PTHR13935. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAAQSAQQQ
60 70 80 90 100
QQQQAPQQQA PQLSPVADGQ PSGGGHKSAA KQVKRQRSSS PELMRCKRRL
110 120 130 140 150
NFSGFGYSLP QQQPAAVARR NERERNRVKL VNLGFATLRE HVPNGAANKK
160 170 180 190 200
MSKVETLRSA VEYIRALQQL LDEHDAVSAA FQAGVLSPTI SPNYSNDLNS
210 220 230
MAGSPVSSYS SDEGSYDPLS PEEQELLDFT NWF
Length:233
Mass (Da):24,972
Last modified:November 1, 1990 - v1
Checksum:i036BDAC8E2D23274
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53725 mRNA. Translation: CAA37760.1.
PIRiS11563.
RefSeqiNP_071779.1. NM_022384.1.
UniGeneiRn.32936.

Genome annotation databases

EnsembliENSRNOT00000005674; ENSRNOP00000005674; ENSRNOG00000004294.
GeneIDi64186.
KEGGirno:64186.
UCSCiRGD:71010. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53725 mRNA. Translation: CAA37760.1.
PIRiS11563.
RefSeqiNP_071779.1. NM_022384.1.
UniGeneiRn.32936.

3D structure databases

ProteinModelPortaliP19359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19359. 1 interaction.
MINTiMINT-3376271.
STRINGi10116.ENSRNOP00000005674.

Proteomic databases

PaxDbiP19359.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005674; ENSRNOP00000005674; ENSRNOG00000004294.
GeneIDi64186.
KEGGirno:64186.
UCSCiRGD:71010. rat.

Organism-specific databases

CTDi429.
RGDi71010. Ascl1.

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00530000063321.
HOGENOMiHOG000013056.
HOVERGENiHBG050590.
InParanoidiP19359.
KOiK09067.
OMAiRCKRRLN.
OrthoDBiEOG7RFTMH.
PhylomeDBiP19359.
TreeFamiTF322889.

Miscellaneous databases

PROiP19359.

Gene expression databases

GenevisibleiP19359. RN.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR015660. Achaete-scute-rel.
IPR011598. bHLH_dom.
[Graphical view]
PANTHERiPTHR13935. PTHR13935. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Two rat homologues of Drosophila achaete-scute specifically expressed in neuronal precursors."
    Johnson J.E., Birren S.J., Anderson D.J.
    Nature 346:858-861(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiASCL1_RAT
AccessioniPrimary (citable) accession number: P19359
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 8, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.