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Protein

Achaete-scute homolog 1

Gene

Ascl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Achaete-scute homolog 1
Gene namesi
Name:Ascl1
Synonyms:Ash1, Mash-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi71010 Ascl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271281 – 233Achaete-scute homolog 1Add BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei153N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP19359

Expressioni

Tissue specificityi

Developing CNS and PNS at embryonic and post-natal stages.1 Publication

Developmental stagei

It is first observed after neurulation, in 10.5 day old (E10.5) rat embryos, and is restricted to subsets of neuroepithelial cells in the spinal cord and the brain, between E10.5 and E13.5. In the periphery, its expression is restricted to some lineages of neural crest-derived cells, namely in sympathetic and enteric neural precursors. In the PNS its expression is extinguished at or before differentiation.1 Publication

Gene expression databases

BgeeiENSRNOG00000004294
GenevisibleiP19359 RN

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3.By similarity

GO - Molecular functioni

  • bHLH transcription factor binding Source: Ensembl
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP19359, 1 interactor
MINTiP19359
STRINGi10116.ENSRNOP00000005674

Structurei

3D structure databases

ProteinModelPortaliP19359
SMRiP19359
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini115 – 167bHLHPROSITE-ProRule annotationAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 44Poly-AlaAdd BLAST15
Compositional biasi48 – 54Poly-Gln7

Phylogenomic databases

eggNOGiKOG4029 Eukaryota
ENOG411227D LUCA
GeneTreeiENSGT00530000063321
HOGENOMiHOG000013056
HOVERGENiHBG050590
InParanoidiP19359
KOiK09067
OMAiELMRCKR
OrthoDBiEOG091G0OB6
PhylomeDBiP19359
TreeFamiTF322889

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR015660 MASH1/Ascl1a-like
PANTHERiPTHR13935 PTHR13935, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

P19359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAAQSAQQQ
60 70 80 90 100
QQQQAPQQQA PQLSPVADGQ PSGGGHKSAA KQVKRQRSSS PELMRCKRRL
110 120 130 140 150
NFSGFGYSLP QQQPAAVARR NERERNRVKL VNLGFATLRE HVPNGAANKK
160 170 180 190 200
MSKVETLRSA VEYIRALQQL LDEHDAVSAA FQAGVLSPTI SPNYSNDLNS
210 220 230
MAGSPVSSYS SDEGSYDPLS PEEQELLDFT NWF
Length:233
Mass (Da):24,972
Last modified:November 1, 1990 - v1
Checksum:i036BDAC8E2D23274
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53725 mRNA Translation: CAA37760.1
PIRiS11563
RefSeqiNP_071779.1, NM_022384.1
UniGeneiRn.32936

Genome annotation databases

EnsembliENSRNOT00000005674; ENSRNOP00000005674; ENSRNOG00000004294
GeneIDi64186
KEGGirno:64186
UCSCiRGD:71010 rat

Similar proteinsi

Entry informationi

Entry nameiASCL1_RAT
AccessioniPrimary (citable) accession number: P19359
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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