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Protein

Solute carrier family 2, facilitated glucose transporter member 4

Gene

Slc2a4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Insulin-regulated facilitative glucose transporter.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei333 – 3331MonosaccharideBy similarity
Binding sitei404 – 4041MonosaccharideBy similarity

GO - Molecular functioni

  1. D-glucose transmembrane transporter activity Source: RGD
  2. glucose transmembrane transporter activity Source: RGD

GO - Biological processi

  1. amylopectin biosynthetic process Source: Ensembl
  2. brown fat cell differentiation Source: Ensembl
  3. cellular response to hypoxia Source: RGD
  4. cellular response to insulin stimulus Source: UniProtKB
  5. cellular response to osmotic stress Source: Ensembl
  6. glucose homeostasis Source: Ensembl
  7. glucose transport Source: RGD
  8. response to ethanol Source: RGD
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

ReactomeiREACT_271665. Facilitative Na+-independent glucose transporters.
REACT_275920. Glucose transport.
REACT_347820. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 2, facilitated glucose transporter member 4
Alternative name(s):
Glucose transporter type 4, insulin-responsive
Short name:
GLUT-4
Gene namesi
Name:Slc2a4
Synonyms:Glut-4, Glut4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2711. Slc2a4.

Subcellular locationi

  1. Cell membrane By similarity; Multi-pass membrane protein By similarity
  2. Endomembrane system By similarity; Multi-pass membrane protein By similarity
  3. Cytoplasmperinuclear region By similarity

  4. Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei25 – 4521Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini46 – 8136ExtracellularSequence AnalysisAdd
BLAST
Transmembranei82 – 10221Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini103 – 1119CytoplasmicSequence Analysis
Transmembranei112 – 13221Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini133 – 14210ExtracellularSequence Analysis
Transmembranei143 – 16321Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini164 – 1718CytoplasmicSequence Analysis
Transmembranei172 – 19221Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini193 – 2019ExtracellularSequence Analysis
Transmembranei202 – 22221Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini223 – 28765CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei288 – 30821Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini309 – 32315ExtracellularSequence AnalysisAdd
BLAST
Transmembranei324 – 34421Helical; Name=8Sequence AnalysisAdd
BLAST
Topological domaini345 – 3539CytoplasmicSequence Analysis
Transmembranei354 – 37421Helical; Name=9Sequence AnalysisAdd
BLAST
Topological domaini375 – 38410ExtracellularSequence Analysis
Transmembranei385 – 40521Helical; Name=10Sequence AnalysisAdd
BLAST
Topological domaini406 – 41712CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei418 – 43821Helical; Name=11Sequence AnalysisAdd
BLAST
Topological domaini439 – 4457ExtracellularSequence Analysis
Transmembranei446 – 46621Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini467 – 50943CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: RGD
  2. coated pit Source: RGD
  3. cytosol Source: Ensembl
  4. endomembrane system Source: UniProtKB
  5. endosome Source: RGD
  6. external side of plasma membrane Source: Ensembl
  7. extracellular vesicular exosome Source: Ensembl
  8. insulin-responsive compartment Source: RGD
  9. integral component of plasma membrane Source: Ensembl
  10. intracellular membrane-bounded organelle Source: RGD
  11. membrane Source: RGD
  12. membrane raft Source: Ensembl
  13. multivesicular body Source: RGD
  14. perinuclear region of cytoplasm Source: RGD
  15. plasma membrane Source: RGD
  16. sarcolemma Source: RGD
  17. trans-Golgi network transport vesicle Source: Ensembl
  18. T-tubule Source: RGD
  19. vesicle membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Involvement in diseasei

It is a candidate for certain post-receptor defects in non-insulin-dependent diabetes mellitus.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Solute carrier family 2, facilitated glucose transporter member 4PRO_0000050366Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
Modified residuei274 – 2741Phosphoserine; by SGK1By similarity

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

PTM databases

PhosphoSiteiP19357.

Expressioni

Tissue specificityi

Skeletal and cardiac muscles; brown and white fat.

Gene expression databases

GenevestigatoriP19357.

Interactioni

Subunit structurei

Binds to DAXX. Interacts via its N-terminus with SRFBP1 (By similarity). Interacts with NDUFA9.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Actn4Q9QXQ03EBI-915426,EBI-919056
GapdhP047972EBI-915426,EBI-349219

Protein-protein interaction databases

BioGridi247208. 1 interaction.
IntActiP19357. 633 interactions.
MINTiMINT-1775053.
STRINGi10116.ENSRNOP00000023256.

Structurei

3D structure databases

ProteinModelPortaliP19357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 137SRFBP1-bindingBy similarity
Regioni298 – 3047Monosaccharide bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi489 – 4902Dileucine internalization motifSequence Analysis

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00760000119022.
HOGENOMiHOG000202871.
HOVERGENiHBG014816.
InParanoidiP19357.
KOiK07191.
OMAiAMLVNNI.
OrthoDBiEOG7QVM2R.
PhylomeDBiP19357.
TreeFamiTF313762.

Family and domain databases

InterProiIPR002441. Glc_transpt_4.
IPR020846. MFS_dom.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR01193. GLUCTRSPORT4.
PR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19357-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSGFQQIGS EDGEPPQQRV TGTLVLAVFS AVLGSLQFGY NIGVINAPQK
60 70 80 90 100
VIEQSYNATW LGRQGPGGPD SIPQGTLTTL WALSVAIFSV GGMISSFLIG
110 120 130 140 150
IISQWLGRKR AMLANNVLAV LGGALMGLAN AAASYEILIL GRFLIGAYSG
160 170 180 190 200
LTSGLVPMYV GEIAPTHLRG ALGTLNQLAI VIGILVAQVL GLESMLGTAT
210 220 230 240 250
LWPLLLAITV LPALLQLLLL PFCPESPRYL YIIRNLEGPA RKSLKRLTGW
260 270 280 290 300
ADVSDALAEL KDEKRKLERE RPLSLLQLLG SRTHRQPLII AVVLQLSQQL
310 320 330 340 350
SGINAVFYYS TSIFELAGVE QPAYATIGAG VVNTVFTLVS VLLVERAGRR
360 370 380 390 400
TLHLLGLAGM CGCAILMTVA LLLLERVPSM SYVSIVAIFG FVAFFEIGPG
410 420 430 440 450
PIPWFIVAEL FSQGPRPAAM AVAGFSNWTC NFIVGMGFQY VADAMGPYVF
460 470 480 490 500
LLFAVLLLGF FIFTFLRVPE TRGRTFDQIS ATFRRTPSLL EQEVKPSTEL

EYLGPDEND
Length:509
Mass (Da):54,896
Last modified:November 1, 1990 - v1
Checksum:iDA50321E99E4CA90
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti349 – 3491R → Q in AAA41451 (PubMed:2649253).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25482 mRNA. Translation: AAA41451.1.
X14771 mRNA. Translation: CAA32879.1.
J04524 mRNA. Translation: AAA41453.1.
D28561 mRNA. Translation: BAA05911.1.
BC085757 mRNA. Translation: AAH85757.1.
L36125 Genomic DNA. Translation: AAA65751.1.
S77784 mRNA. No translation available.
PIRiA32101.
RefSeqiNP_036883.1. NM_012751.1.
XP_006246658.1. XM_006246596.2.
UniGeneiRn.1314.

Genome annotation databases

EnsembliENSRNOT00000023256; ENSRNOP00000023256; ENSRNOG00000017226.
GeneIDi25139.
KEGGirno:25139.
UCSCiRGD:2711. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25482 mRNA. Translation: AAA41451.1.
X14771 mRNA. Translation: CAA32879.1.
J04524 mRNA. Translation: AAA41453.1.
D28561 mRNA. Translation: BAA05911.1.
BC085757 mRNA. Translation: AAH85757.1.
L36125 Genomic DNA. Translation: AAA65751.1.
S77784 mRNA. No translation available.
PIRiA32101.
RefSeqiNP_036883.1. NM_012751.1.
XP_006246658.1. XM_006246596.2.
UniGeneiRn.1314.

3D structure databases

ProteinModelPortaliP19357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247208. 1 interaction.
IntActiP19357. 633 interactions.
MINTiMINT-1775053.
STRINGi10116.ENSRNOP00000023256.

Chemistry

ChEMBLiCHEMBL3286066.

PTM databases

PhosphoSiteiP19357.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023256; ENSRNOP00000023256; ENSRNOG00000017226.
GeneIDi25139.
KEGGirno:25139.
UCSCiRGD:2711. rat.

Organism-specific databases

CTDi6517.
RGDi2711. Slc2a4.

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00760000119022.
HOGENOMiHOG000202871.
HOVERGENiHBG014816.
InParanoidiP19357.
KOiK07191.
OMAiAMLVNNI.
OrthoDBiEOG7QVM2R.
PhylomeDBiP19357.
TreeFamiTF313762.

Enzyme and pathway databases

ReactomeiREACT_271665. Facilitative Na+-independent glucose transporters.
REACT_275920. Glucose transport.
REACT_347820. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

NextBioi605563.
PROiP19357.

Gene expression databases

GenevestigatoriP19357.

Family and domain databases

InterProiIPR002441. Glc_transpt_4.
IPR020846. MFS_dom.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR01193. GLUCTRSPORT4.
PR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel gene encoding an insulin-responsive glucose transporter protein."
    Birnbaum M.J.
    Cell 57:305-315(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning and characterization of an insulin-regulatable glucose transporter."
    James D.E., Strube M., Mueckler M.
    Nature 338:83-87(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "A glucose transport protein expressed predominately in insulin-responsive tissues."
    Charron M.J., Brosius F.C. III, Alper S.L., Lodish H.F.
    Proc. Natl. Acad. Sci. U.S.A. 86:2535-2539(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Characterization of rat Glut4 glucose transporter expressed in the yeast Saccharomyces cerevisiae: comparison with Glut1 glucose transporter."
    Kasahara T., Kasahara M.
    Biochim. Biophys. Acta 1324:111-119(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  6. "Myocyte enhancer factor 2 (MEF2) binding site is essential for C2C12 myotube-specific expression of the rat GLUT4/muscle-adipose facilitative glucose transporter gene."
    Liu M.L., Olson A.L., Edgington N.P., Moye-Rowley W.S., Pessin J.E.
    J. Biol. Chem. 269:28514-28521(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
  7. "Characterization of 5'-heterogeneity of the rat GLUT4/muscle-adipose glucose transporter gene product."
    Olson A.L., Edgington N.P., Moye-Rowley W.S., Pessin J.E.
    Endocrinology 136:1962-1968(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-25.
  8. "Intracellular targeting of the insulin-regulatable glucose transporter (GLUT4) is isoform specific and independent of cell type."
    Haney P.M., Slot J.W., Piper R.C., James D.E., Mueckler M.
    J. Cell Biol. 114:689-699(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "Insulin-dependent interactions of proteins with GLUT4 revealed through stable isotope labeling by amino acids in cell culture (SILAC)."
    Foster L.J., Rudich A., Talior I., Patel N., Huang X., Furtado L.M., Bilan P.J., Mann M., Klip A.
    J. Proteome Res. 5:64-75(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NDUFA9.

Entry informationi

Entry nameiGTR4_RAT
AccessioniPrimary (citable) accession number: P19357
Secondary accession number(s): P97900
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 1, 2015
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Insulin-stimulated phosphorylation of TBC1D4 is required for GLUT4 translocation.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.