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P19357

- GTR4_RAT

UniProt

P19357 - GTR4_RAT

Protein

Solute carrier family 2, facilitated glucose transporter member 4

Gene

Slc2a4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Insulin-regulated facilitative glucose transporter.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei333 – 3331MonosaccharideBy similarity
    Binding sitei404 – 4041MonosaccharideBy similarity

    GO - Molecular functioni

    1. D-glucose transmembrane transporter activity Source: RGD
    2. glucose transmembrane transporter activity Source: RGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. amylopectin biosynthetic process Source: Ensembl
    2. brown fat cell differentiation Source: Ensembl
    3. cellular response to hypoxia Source: RGD
    4. cellular response to insulin stimulus Source: UniProtKB
    5. cellular response to osmotic stress Source: Ensembl
    6. glucose homeostasis Source: Ensembl
    7. glucose transport Source: RGD
    8. response to ethanol Source: RGD

    Keywords - Biological processi

    Sugar transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_199176. Translocation of GLUT4 to the plasma membrane.
    REACT_205960. Glucose transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 2, facilitated glucose transporter member 4
    Alternative name(s):
    Glucose transporter type 4, insulin-responsive
    Short name:
    GLUT-4
    Gene namesi
    Name:Slc2a4
    Synonyms:Glut-4, Glut4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi2711. Slc2a4.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Endomembrane system By similarity; Multi-pass membrane protein By similarity. Cytoplasmperinuclear region By similarity
    Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration By similarity.By similarity

    GO - Cellular componenti

    1. cell surface Source: RGD
    2. coated pit Source: RGD
    3. endomembrane system Source: UniProtKB
    4. endosome Source: RGD
    5. external side of plasma membrane Source: Ensembl
    6. extracellular vesicular exosome Source: Ensembl
    7. insulin-responsive compartment Source: RGD
    8. integral component of plasma membrane Source: Ensembl
    9. intracellular membrane-bounded organelle Source: RGD
    10. membrane Source: RGD
    11. multivesicular body Source: RGD
    12. perinuclear region of cytoplasm Source: RGD
    13. plasma membrane Source: RGD
    14. sarcolemma Source: RGD
    15. trans-Golgi network transport vesicle Source: Ensembl
    16. T-tubule Source: RGD
    17. vesicle membrane Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    It is a candidate for certain post-receptor defects in non-insulin-dependent diabetes mellitus.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 509509Solute carrier family 2, facilitated glucose transporter member 4PRO_0000050366Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
    Modified residuei274 – 2741Phosphoserine; by SGK1By similarity

    Post-translational modificationi

    Sumoylated.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein, Ubl conjugation

    PTM databases

    PhosphoSiteiP19357.

    Expressioni

    Tissue specificityi

    Skeletal and cardiac muscles; brown and white fat.

    Gene expression databases

    GenevestigatoriP19357.

    Interactioni

    Subunit structurei

    Binds to DAXX. Interacts via its N-terminus with SRFBP1 By similarity. Interacts with NDUFA9.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Actn4Q9QXQ03EBI-915426,EBI-919056
    GapdhP047972EBI-915426,EBI-349219

    Protein-protein interaction databases

    BioGridi247208. 1 interaction.
    IntActiP19357. 633 interactions.
    MINTiMINT-1775053.
    STRINGi10116.ENSRNOP00000023256.

    Structurei

    3D structure databases

    ProteinModelPortaliP19357.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2424CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini46 – 8136ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini103 – 1119CytoplasmicSequence Analysis
    Topological domaini133 – 14210ExtracellularSequence Analysis
    Topological domaini164 – 1718CytoplasmicSequence Analysis
    Topological domaini193 – 2019ExtracellularSequence Analysis
    Topological domaini223 – 28765CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini309 – 32315ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini345 – 3539CytoplasmicSequence Analysis
    Topological domaini375 – 38410ExtracellularSequence Analysis
    Topological domaini406 – 41712CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini439 – 4457ExtracellularSequence Analysis
    Topological domaini467 – 50943CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4521Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei82 – 10221Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei112 – 13221Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei143 – 16321Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei172 – 19221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei202 – 22221Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei288 – 30821Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei324 – 34421Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei354 – 37421Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei385 – 40521Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei418 – 43821Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei446 – 46621Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni7 – 137SRFBP1-bindingBy similarity
    Regioni298 – 3047Monosaccharide bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi489 – 4902Dileucine internalization motifSequence Analysis

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00720000108560.
    HOGENOMiHOG000202871.
    HOVERGENiHBG014816.
    KOiK07191.
    OMAiAMLVNNI.
    OrthoDBiEOG7QVM2R.
    PhylomeDBiP19357.
    TreeFamiTF313762.

    Family and domain databases

    InterProiIPR002441. Glc_transpt_4.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    PRINTSiPR01193. GLUCTRSPORT4.
    PR00171. SUGRTRNSPORT.
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00879. SP. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19357-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSGFQQIGS EDGEPPQQRV TGTLVLAVFS AVLGSLQFGY NIGVINAPQK    50
    VIEQSYNATW LGRQGPGGPD SIPQGTLTTL WALSVAIFSV GGMISSFLIG 100
    IISQWLGRKR AMLANNVLAV LGGALMGLAN AAASYEILIL GRFLIGAYSG 150
    LTSGLVPMYV GEIAPTHLRG ALGTLNQLAI VIGILVAQVL GLESMLGTAT 200
    LWPLLLAITV LPALLQLLLL PFCPESPRYL YIIRNLEGPA RKSLKRLTGW 250
    ADVSDALAEL KDEKRKLERE RPLSLLQLLG SRTHRQPLII AVVLQLSQQL 300
    SGINAVFYYS TSIFELAGVE QPAYATIGAG VVNTVFTLVS VLLVERAGRR 350
    TLHLLGLAGM CGCAILMTVA LLLLERVPSM SYVSIVAIFG FVAFFEIGPG 400
    PIPWFIVAEL FSQGPRPAAM AVAGFSNWTC NFIVGMGFQY VADAMGPYVF 450
    LLFAVLLLGF FIFTFLRVPE TRGRTFDQIS ATFRRTPSLL EQEVKPSTEL 500
    EYLGPDEND 509
    Length:509
    Mass (Da):54,896
    Last modified:November 1, 1990 - v1
    Checksum:iDA50321E99E4CA90
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti349 – 3491R → Q in AAA41451. (PubMed:2649253)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25482 mRNA. Translation: AAA41451.1.
    X14771 mRNA. Translation: CAA32879.1.
    J04524 mRNA. Translation: AAA41453.1.
    D28561 mRNA. Translation: BAA05911.1.
    BC085757 mRNA. Translation: AAH85757.1.
    L36125 Genomic DNA. Translation: AAA65751.1.
    S77784 mRNA. No translation available.
    PIRiA32101.
    RefSeqiNP_036883.1. NM_012751.1.
    XP_006246658.1. XM_006246596.1.
    UniGeneiRn.1314.

    Genome annotation databases

    EnsembliENSRNOT00000023256; ENSRNOP00000023256; ENSRNOG00000017226.
    GeneIDi25139.
    KEGGirno:25139.
    UCSCiRGD:2711. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M25482 mRNA. Translation: AAA41451.1 .
    X14771 mRNA. Translation: CAA32879.1 .
    J04524 mRNA. Translation: AAA41453.1 .
    D28561 mRNA. Translation: BAA05911.1 .
    BC085757 mRNA. Translation: AAH85757.1 .
    L36125 Genomic DNA. Translation: AAA65751.1 .
    S77784 mRNA. No translation available.
    PIRi A32101.
    RefSeqi NP_036883.1. NM_012751.1.
    XP_006246658.1. XM_006246596.1.
    UniGenei Rn.1314.

    3D structure databases

    ProteinModelPortali P19357.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247208. 1 interaction.
    IntActi P19357. 633 interactions.
    MINTi MINT-1775053.
    STRINGi 10116.ENSRNOP00000023256.

    PTM databases

    PhosphoSitei P19357.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000023256 ; ENSRNOP00000023256 ; ENSRNOG00000017226 .
    GeneIDi 25139.
    KEGGi rno:25139.
    UCSCi RGD:2711. rat.

    Organism-specific databases

    CTDi 6517.
    RGDi 2711. Slc2a4.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00720000108560.
    HOGENOMi HOG000202871.
    HOVERGENi HBG014816.
    KOi K07191.
    OMAi AMLVNNI.
    OrthoDBi EOG7QVM2R.
    PhylomeDBi P19357.
    TreeFami TF313762.

    Enzyme and pathway databases

    Reactomei REACT_199176. Translocation of GLUT4 to the plasma membrane.
    REACT_205960. Glucose transport.

    Miscellaneous databases

    NextBioi 605563.
    PROi P19357.

    Gene expression databases

    Genevestigatori P19357.

    Family and domain databases

    InterProi IPR002441. Glc_transpt_4.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    PRINTSi PR01193. GLUCTRSPORT4.
    PR00171. SUGRTRNSPORT.
    SUPFAMi SSF103473. SSF103473. 1 hit.
    TIGRFAMsi TIGR00879. SP. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel gene encoding an insulin-responsive glucose transporter protein."
      Birnbaum M.J.
      Cell 57:305-315(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning and characterization of an insulin-regulatable glucose transporter."
      James D.E., Strube M., Mueckler M.
      Nature 338:83-87(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "A glucose transport protein expressed predominately in insulin-responsive tissues."
      Charron M.J., Brosius F.C. III, Alper S.L., Lodish H.F.
      Proc. Natl. Acad. Sci. U.S.A. 86:2535-2539(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Characterization of rat Glut4 glucose transporter expressed in the yeast Saccharomyces cerevisiae: comparison with Glut1 glucose transporter."
      Kasahara T., Kasahara M.
      Biochim. Biophys. Acta 1324:111-119(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.
    6. "Myocyte enhancer factor 2 (MEF2) binding site is essential for C2C12 myotube-specific expression of the rat GLUT4/muscle-adipose facilitative glucose transporter gene."
      Liu M.L., Olson A.L., Edgington N.P., Moye-Rowley W.S., Pessin J.E.
      J. Biol. Chem. 269:28514-28521(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
    7. "Characterization of 5'-heterogeneity of the rat GLUT4/muscle-adipose glucose transporter gene product."
      Olson A.L., Edgington N.P., Moye-Rowley W.S., Pessin J.E.
      Endocrinology 136:1962-1968(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-25.
    8. "Intracellular targeting of the insulin-regulatable glucose transporter (GLUT4) is isoform specific and independent of cell type."
      Haney P.M., Slot J.W., Piper R.C., James D.E., Mueckler M.
      J. Cell Biol. 114:689-699(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "Insulin-dependent interactions of proteins with GLUT4 revealed through stable isotope labeling by amino acids in cell culture (SILAC)."
      Foster L.J., Rudich A., Talior I., Patel N., Huang X., Furtado L.M., Bilan P.J., Mann M., Klip A.
      J. Proteome Res. 5:64-75(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NDUFA9.

    Entry informationi

    Entry nameiGTR4_RAT
    AccessioniPrimary (citable) accession number: P19357
    Secondary accession number(s): P97900
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Insulin-stimulated phosphorylation of TBC1D4 is required for GLUT4 translocation.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3