Solute carrier family 2, facilitated glucose transporter member 4

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Reviewed, UniProtKB/Swiss-Prot P19357 (GTR4_RAT)

Last modified October 13, 2009. Version 96. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Solute carrier family 2, facilitated glucose transporter member 4
Alternative name(s):
    Glucose transporter type 4, insulin-responsive
      Short name=GLUT-4

Gene names

Name:	Slc2a4
Synonyms:	Glut-4, Glut4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Insulin-regulated facilitative glucose transporter.
Subunit structure	Binds to DAXX. Interacts via its N-terminus with SRFBP1 By similarity. Interacts with NDUFA9.
Subcellular location	Endomembrane system; Multi-pass membrane protein By similarity. Cytoplasm › perinuclear region By similarity. Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration By similarity.
Tissue specificity	Skeletal and cardiac muscles; brown and white fat.
Post-translational modification	Sumoylated By similarity.
Involvement in disease	It is a candidate for certain post-receptor defects in non-insulin-dependent diabetes mellitus.
Miscellaneous	Insulin-stimulated phosphorylation of TBC1D4 is required for GLUT4 translocation By similarity.
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. [View classification]

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Ontologies

Keywords
Biological process	Sugar transport Transport
Cellular component	Cytoplasm Membrane
Domain	Transmembrane
PTM	Glycoprotein Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	glucose transport Ref.4 Inferred from direct assay. Source: RGD transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	coated pit Inferred from direct assay. Source: RGD insulin-responsive compartment Inferred from direct assay. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from direct assay. Source: RGD multivesicular body Inferred from direct assay. Source: RGD perinuclear region of cytoplasm Inferred from direct assay. Source: RGD sarcolemma Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	D-glucose transmembrane transporter activity Inferred from direct assay. Source: RGD protein binding Ref.9 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
Actn4	Q9QXQ0	1	EBI-915426,EBI-919056
Gapdh	P04797	1	EBI-915426,EBI-349219

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 509

509

Solute carrier family 2, facilitated glucose transporter member 4

PRO_0000050366

Regions

Topological domain

1 – 24

Cytoplasmic Potential

Transmembrane

25 – 45

1 Potential

Topological domain

46 – 81

Extracellular Potential

Transmembrane

82 – 102

2 Potential

Topological domain

103 – 111

Cytoplasmic Potential

Transmembrane

112 – 132

3 Potential

Topological domain

133 – 142

Extracellular Potential

Transmembrane

143 – 163

4 Potential

Topological domain

164 – 171

Cytoplasmic Potential

Transmembrane

172 – 192

5 Potential

Topological domain

193 – 201

Extracellular Potential

Transmembrane

202 – 222

6 Potential

Topological domain

223 – 287

Cytoplasmic Potential

Transmembrane

288 – 308

7 Potential

Topological domain

309 – 323

Extracellular Potential

Transmembrane

324 – 344

8 Potential

Topological domain

345 – 353

Cytoplasmic Potential

Transmembrane

354 – 374

9 Potential

Topological domain

375 – 384

Extracellular Potential

Transmembrane

385 – 405

10 Potential

Topological domain

406 – 417

Cytoplasmic Potential

Transmembrane

418 – 438

11 Potential

Topological domain

439 – 445

Extracellular Potential

Transmembrane

446 – 466

12 Potential

Topological domain

467 – 509

Cytoplasmic Potential

Region

7 – 13

SRFBP1-binding By similarity

Region

295 – 297

Defines substrate specificity By similarity

Motif

489 – 490

Dileucine internalization motif Potential

Amino acid modifications

Modified residue

488

Phosphoserine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

349

R → Q in AAA41451. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P19357-1 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: DA50321E99E4CA90
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FASTA

509

54,896

        10         20         30         40         50         60 
MPSGFQQIGS EDGEPPQQRV TGTLVLAVFS AVLGSLQFGY NIGVINAPQK VIEQSYNATW 

        70         80         90        100        110        120 
LGRQGPGGPD SIPQGTLTTL WALSVAIFSV GGMISSFLIG IISQWLGRKR AMLANNVLAV 

       130        140        150        160        170        180 
LGGALMGLAN AAASYEILIL GRFLIGAYSG LTSGLVPMYV GEIAPTHLRG ALGTLNQLAI 

       190        200        210        220        230        240 
VIGILVAQVL GLESMLGTAT LWPLLLAITV LPALLQLLLL PFCPESPRYL YIIRNLEGPA 

       250        260        270        280        290        300 
RKSLKRLTGW ADVSDALAEL KDEKRKLERE RPLSLLQLLG SRTHRQPLII AVVLQLSQQL 

       310        320        330        340        350        360 
SGINAVFYYS TSIFELAGVE QPAYATIGAG VVNTVFTLVS VLLVERAGRR TLHLLGLAGM 

       370        380        390        400        410        420 
CGCAILMTVA LLLLERVPSM SYVSIVAIFG FVAFFEIGPG PIPWFIVAEL FSQGPRPAAM 

       430        440        450        460        470        480 
AVAGFSNWTC NFIVGMGFQY VADAMGPYVF LLFAVLLLGF FIFTFLRVPE TRGRTFDQIS 

       490        500 
ATFRRTPSLL EQEVKPSTEL EYLGPDEND

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel gene encoding an insulin-responsive glucose transporter protein." Birnbaum M.J. Cell 57:305-315(1989) [PubMed: 2649253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and characterization of an insulin-regulatable glucose transporter." James D.E., Strube M., Mueckler M. Nature 338:83-87(1989) [PubMed: 2645527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"A glucose transport protein expressed predominately in insulin-responsive tissues." Charron M.J., Brosius F.C. III, Alper S.L., Lodish H.F. Proc. Natl. Acad. Sci. U.S.A. 86:2535-2539(1989) [PubMed: 2649883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Characterization of rat Glut4 glucose transporter expressed in the yeast Saccharomyces cerevisiae: comparison with Glut1 glucose transporter." Kasahara T., Kasahara M. Biochim. Biophys. Acta 1324:111-119(1997) [PubMed: 9059504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[6]	"Myocyte enhancer factor 2 (MEF2) binding site is essential for C2C12 myotube-specific expression of the rat GLUT4/muscle-adipose facilitative glucose transporter gene." Liu M.L., Olson A.L., Edgington N.P., Moye-Rowley W.S., Pessin J.E. J. Biol. Chem. 269:28514-28521(1994) [PubMed: 7545962] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
[7]	"Characterization of 5'-heterogeneity of the rat GLUT4/muscle-adipose glucose transporter gene product." Olson A.L., Edgington N.P., Moye-Rowley W.S., Pessin J.E. Endocrinology 136:1962-1968(1995) [PubMed: 7720644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-25.
[8]	"Intracellular targeting of the insulin-regulatable glucose transporter (GLUT4) is isoform specific and independent of cell type." Haney P.M., Slot J.W., Piper R.C., James D.E., Mueckler M. J. Cell Biol. 114:689-699(1991) [PubMed: 1651337] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Insulin-dependent interactions of proteins with GLUT4 revealed through stable isotope labeling by amino acids in cell culture (SILAC)." Foster L.J., Rudich A., Talior I., Patel N., Huang X., Furtado L.M., Bilan P.J., Mann M., Klip A. J. Proteome Res. 5:64-75(2006) [PubMed: 16396496] [Abstract] Cited for: INTERACTION WITH NDUFA9.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	M25482 mRNA. Translation: AAA41451.1. X14771 mRNA. Translation: CAA32879.1. J04524 mRNA. Translation: AAA41453.1. D28561 mRNA. Translation: BAA05911.1. BC085757 mRNA. Translation: AAH85757.1. L36125 Genomic DNA. Translation: AAA65751.1. S77784 mRNA. No translation available.
IPI	IPI00194348.
PIR	A32101.
RefSeq	NP_036883.1.
UniGene	Rn.1314
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P19357. 634 interactions.
STRING	P19357.
PTM databases
PhosphoSite	P19357.
Genome annotation databases
Ensembl	ENSRNOT00000023256; ENSRNOP00000023256; ENSRNOG00000017226; Rattus norvegicus. [Genome view] ENSRNOT00000056825; ENSRNOP00000053668; ENSRNOG00000017226; Rattus norvegicus. [Genome view]
GeneID	25139.
KEGG	rno:25139.
UCSC	NM_012751. rat.
Organism-specific databases
CTD	25139.
RGD	2711. Slc2a4.
Phylogenomic databases
HOVERGEN	P19357.
Gene expression databases
ArrayExpress	P19357.
Genevestigator	P19357.
GermOnline	ENSRNOG00000017226. Rattus norvegicus.
Family and domain databases
InterPro	IPR002441. Glc_transpt_4. IPR005828. Sub_transporter. IPR003663. Sugar/inositol_transpt. IPR005829. Sugar_transporter_CS. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
PRINTS	PR01193. GLUCTRSPORT4. PR00171. SUGRTRNSPORT.
TIGRFAMs	TIGR00879. SP. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. 1 hit. PS00217. SUGAR_TRANSPORT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	605563.

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Entry information

Entry name

GTR4_RAT

Accession

Primary (citable) accession number: P19357
Secondary accession number(s): P97900

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	October 13, 2009
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents