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P19357 (GTR4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 2, facilitated glucose transporter member 4
Alternative name(s):
Glucose transporter type 4, insulin-responsive
Short name=GLUT-4
Gene names
Name:Slc2a4
Synonyms:Glut-4, Glut4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Insulin-regulated facilitative glucose transporter.

Subunit structure

Binds to DAXX. Interacts via its N-terminus with SRFBP1 By similarity. Interacts with NDUFA9. Ref.9

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Endomembrane system; Multi-pass membrane protein By similarity. Cytoplasmperinuclear region By similarity. Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration By similarity. Ref.8

Tissue specificity

Skeletal and cardiac muscles; brown and white fat.

Post-translational modification

Sumoylated By similarity.

Involvement in disease

It is a candidate for certain post-receptor defects in non-insulin-dependent diabetes mellitus.

Miscellaneous

Insulin-stimulated phosphorylation of TBC1D4 is required for GLUT4 translocation By similarity.

Sequence similarities

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. [View classification]

Ontologies

Keywords
   Biological processSugar transport
Transport
   Cellular componentCell membrane
Cytoplasm
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processamylopectin biosynthetic process

Inferred from electronic annotation. Source: Ensembl

brown fat cell differentiation

Inferred from electronic annotation. Source: Ensembl

cellular response to hypoxia

Inferred from direct assay PubMed 20868366. Source: RGD

cellular response to insulin stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to osmotic stress

Inferred from electronic annotation. Source: Ensembl

glucose homeostasis

Inferred from electronic annotation. Source: Ensembl

glucose transport

Inferred from direct assay PubMed 1733237PubMed 2211693. Source: RGD

response to ethanol

Inferred from expression pattern PubMed 18959809. Source: RGD

   Cellular_componentT-tubule

Inferred from direct assay PubMed 20868366. Source: RGD

cell surface

Inferred from direct assay PubMed 20868366. Source: RGD

coated pit

Inferred from direct assay PubMed 1881917. Source: RGD

endomembrane system

Inferred from sequence or structural similarity. Source: UniProtKB

endosome

Inferred from direct assay PubMed 12450403. Source: RGD

external side of plasma membrane

Inferred from electronic annotation. Source: Ensembl

extracellular vesicular exosome

Inferred from electronic annotation. Source: Ensembl

insulin-responsive compartment

Inferred from direct assay PubMed 8366094PubMed 9271094PubMed 9516411PubMed 9880520. Source: RGD

integral component of plasma membrane

Inferred from electronic annotation. Source: Ensembl

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 10051443PubMed 8037667PubMed 8366094PubMed 8536622PubMed 9224710. Source: RGD

membrane

Inferred from direct assay PubMed 11500317. Source: RGD

multivesicular body

Inferred from direct assay PubMed 1881917. Source: RGD

perinuclear region of cytoplasm

Inferred from direct assay PubMed 12388133PubMed 20868366PubMed 9271094. Source: RGD

plasma membrane

Inferred from direct assay PubMed 10051443PubMed 16870704PubMed 2211693PubMed 8037667PubMed 8536622PubMed 9224710. Source: RGD

sarcolemma

Inferred from direct assay PubMed 12388133PubMed 1881917PubMed 20868366. Source: RGD

trans-Golgi network transport vesicle

Inferred from electronic annotation. Source: Ensembl

vesicle membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionD-glucose transmembrane transporter activity

Inferred from direct assay PubMed 1733237PubMed 2211693. Source: RGD

glucose transmembrane transporter activity

Inferred from direct assay PubMed 11500317. Source: RGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Actn4Q9QXQ03EBI-915426,EBI-919056
GapdhP047972EBI-915426,EBI-349219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 509509Solute carrier family 2, facilitated glucose transporter member 4
PRO_0000050366

Regions

Topological domain1 – 2424Cytoplasmic Potential
Transmembrane25 – 4521Helical; Name=1; Potential
Topological domain46 – 8136Extracellular Potential
Transmembrane82 – 10221Helical; Name=2; Potential
Topological domain103 – 1119Cytoplasmic Potential
Transmembrane112 – 13221Helical; Name=3; Potential
Topological domain133 – 14210Extracellular Potential
Transmembrane143 – 16321Helical; Name=4; Potential
Topological domain164 – 1718Cytoplasmic Potential
Transmembrane172 – 19221Helical; Name=5; Potential
Topological domain193 – 2019Extracellular Potential
Transmembrane202 – 22221Helical; Name=6; Potential
Topological domain223 – 28765Cytoplasmic Potential
Transmembrane288 – 30821Helical; Name=7; Potential
Topological domain309 – 32315Extracellular Potential
Transmembrane324 – 34421Helical; Name=8; Potential
Topological domain345 – 3539Cytoplasmic Potential
Transmembrane354 – 37421Helical; Name=9; Potential
Topological domain375 – 38410Extracellular Potential
Transmembrane385 – 40521Helical; Name=10; Potential
Topological domain406 – 41712Cytoplasmic Potential
Transmembrane418 – 43821Helical; Name=11; Potential
Topological domain439 – 4457Extracellular Potential
Transmembrane446 – 46621Helical; Name=12; Potential
Topological domain467 – 50943Cytoplasmic Potential
Region7 – 137SRFBP1-binding By similarity
Region295 – 2973Defines substrate specificity By similarity
Motif489 – 4902Dileucine internalization motif Potential

Amino acid modifications

Modified residue2741Phosphoserine; by SGK1 By similarity
Glycosylation571N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3491R → Q in AAA41451. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P19357 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: DA50321E99E4CA90

FASTA50954,896
        10         20         30         40         50         60 
MPSGFQQIGS EDGEPPQQRV TGTLVLAVFS AVLGSLQFGY NIGVINAPQK VIEQSYNATW 

        70         80         90        100        110        120 
LGRQGPGGPD SIPQGTLTTL WALSVAIFSV GGMISSFLIG IISQWLGRKR AMLANNVLAV 

       130        140        150        160        170        180 
LGGALMGLAN AAASYEILIL GRFLIGAYSG LTSGLVPMYV GEIAPTHLRG ALGTLNQLAI 

       190        200        210        220        230        240 
VIGILVAQVL GLESMLGTAT LWPLLLAITV LPALLQLLLL PFCPESPRYL YIIRNLEGPA 

       250        260        270        280        290        300 
RKSLKRLTGW ADVSDALAEL KDEKRKLERE RPLSLLQLLG SRTHRQPLII AVVLQLSQQL 

       310        320        330        340        350        360 
SGINAVFYYS TSIFELAGVE QPAYATIGAG VVNTVFTLVS VLLVERAGRR TLHLLGLAGM 

       370        380        390        400        410        420 
CGCAILMTVA LLLLERVPSM SYVSIVAIFG FVAFFEIGPG PIPWFIVAEL FSQGPRPAAM 

       430        440        450        460        470        480 
AVAGFSNWTC NFIVGMGFQY VADAMGPYVF LLFAVLLLGF FIFTFLRVPE TRGRTFDQIS 

       490        500 
ATFRRTPSLL EQEVKPSTEL EYLGPDEND 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a novel gene encoding an insulin-responsive glucose transporter protein."
Birnbaum M.J.
Cell 57:305-315(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning and characterization of an insulin-regulatable glucose transporter."
James D.E., Strube M., Mueckler M.
Nature 338:83-87(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"A glucose transport protein expressed predominately in insulin-responsive tissues."
Charron M.J., Brosius F.C. III, Alper S.L., Lodish H.F.
Proc. Natl. Acad. Sci. U.S.A. 86:2535-2539(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Characterization of rat Glut4 glucose transporter expressed in the yeast Saccharomyces cerevisiae: comparison with Glut1 glucose transporter."
Kasahara T., Kasahara M.
Biochim. Biophys. Acta 1324:111-119(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
[6]"Myocyte enhancer factor 2 (MEF2) binding site is essential for C2C12 myotube-specific expression of the rat GLUT4/muscle-adipose facilitative glucose transporter gene."
Liu M.L., Olson A.L., Edgington N.P., Moye-Rowley W.S., Pessin J.E.
J. Biol. Chem. 269:28514-28521(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
[7]"Characterization of 5'-heterogeneity of the rat GLUT4/muscle-adipose glucose transporter gene product."
Olson A.L., Edgington N.P., Moye-Rowley W.S., Pessin J.E.
Endocrinology 136:1962-1968(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-25.
[8]"Intracellular targeting of the insulin-regulatable glucose transporter (GLUT4) is isoform specific and independent of cell type."
Haney P.M., Slot J.W., Piper R.C., James D.E., Mueckler M.
J. Cell Biol. 114:689-699(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Insulin-dependent interactions of proteins with GLUT4 revealed through stable isotope labeling by amino acids in cell culture (SILAC)."
Foster L.J., Rudich A., Talior I., Patel N., Huang X., Furtado L.M., Bilan P.J., Mann M., Klip A.
J. Proteome Res. 5:64-75(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NDUFA9.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M25482 mRNA. Translation: AAA41451.1.
X14771 mRNA. Translation: CAA32879.1.
J04524 mRNA. Translation: AAA41453.1.
D28561 mRNA. Translation: BAA05911.1.
BC085757 mRNA. Translation: AAH85757.1.
L36125 Genomic DNA. Translation: AAA65751.1.
S77784 mRNA. No translation available.
PIRA32101.
RefSeqNP_036883.1. NM_012751.1.
XP_006246658.1. XM_006246596.1.
UniGeneRn.1314.

3D structure databases

ProteinModelPortalP19357.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid247208. 1 interaction.
IntActP19357. 633 interactions.
MINTMINT-1775053.
STRING10116.ENSRNOP00000023256.

PTM databases

PhosphoSiteP19357.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000023256; ENSRNOP00000023256; ENSRNOG00000017226.
GeneID25139.
KEGGrno:25139.
UCSCRGD:2711. rat.

Organism-specific databases

CTD6517.
RGD2711. Slc2a4.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00720000108560.
HOGENOMHOG000202871.
HOVERGENHBG014816.
KOK07191.
OMAAMLVNNI.
OrthoDBEOG7QVM2R.
PhylomeDBP19357.
TreeFamTF313762.

Gene expression databases

GenevestigatorP19357.

Family and domain databases

InterProIPR002441. Glc_transpt_4.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSPR01193. GLUCTRSPORT4.
PR00171. SUGRTRNSPORT.
SUPFAMSSF103473. SSF103473. 1 hit.
TIGRFAMsTIGR00879. SP. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605563.
PROP19357.

Entry information

Entry nameGTR4_RAT
AccessionPrimary (citable) accession number: P19357
Secondary accession number(s): P97900
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families