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P19337 (BAIA2_CLOSV) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bile acid 7-dehydroxylase 2

EC=1.17.99.5
Alternative name(s):
Bile acid-inducible protein 2
Cholate 7-alpha-dehydroxylase 2
Gene names
Name:baiA2
OrganismClostridium scindens (strain JCM 10418 / VPI 12708)
Taxonomic identifier29347 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length249 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

7-alpha-dehydroxylation of cholic acid and chenodeoxycholate, yielding deoxycholic acid and lithocholic acid, respectively. Highest affinity with taurochenodeoxycholic acid.

Catalytic activity

Deoxycholate + FAD + H2O = cholate + FADH2.

Lithocholate + FAD + H2O = chenodeoxycholate + FADH2.

Pathway

Lipid metabolism; bile acid degradation.

Induction

Presence of C24 bile acids containing a 7-alpha-hydroxy group.

Miscellaneous

There are three genes for BaiA proteins: baiA1 is identical to baiA3 and there is 81% identity with baiA2.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
   Biological processBile acid catabolism
Lipid degradation
Lipid metabolism
Steroid metabolism
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processbile acid catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionbile-acid 7alpha-dehydroxylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 249249Bile acid 7-dehydroxylase 2
PRO_0000054525

Regions

Nucleotide binding7 – 137NAD Potential
Nucleotide binding32 – 365NAD Potential

Sites

Active site1571Proton acceptor By similarity
Binding site1441Substrate By similarity

Secondary structure

............................................. 249
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P19337 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 9A39B78BB63DC5AF

FASTA24926,538
        10         20         30         40         50         60 
MNLVQDKVTI ITGGTRGIGF AAAKIFIDNG AKVSIFGETQ EEVDTALAQL KELYPEEEVL 

        70         80         90        100        110        120 
GFAPDLTSRD AVMAAVGQVA QKYGRLDVMI NNAGITSNNV FSRVSEEEFK HIMDINVTGV 

       130        140        150        160        170        180 
FNGAWCAYQC MKDAKKGVII NTASVTGIFG SLSGVGYPAS KASVIGLTHG LGREIIRKNI 

       190        200        210        220        230        240 
RVVGVAPGVV NTDMTNGNPP EIMEGYLKAL PMKRMLEPEE IANVYLFLAS DLASGITATT 


VSVDGAYRP 

« Hide

References

[1]"Cloning and sequencing of a bile acid-inducible operon from Eubacterium sp. strain VPI 12708."
Mallonee D.H., White W.B., Hylemon P.B.
J. Bacteriol. 172:7011-7019(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Multiple copies of a bile acid-inducible gene in Eubacterium sp. strain VPI 12708."
Gopal-Srivastava R., Mallonee D.H., White W.B., Hylemon P.B.
J. Bacteriol. 172:4420-4426(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Evidence for a multigene family involved in bile acid 7-dehydroxylation in Eubacterium sp. strain VPI 12708."
White W.B., Franklund C.V., Coleman J.P., Hylemon P.B.
J. Bacteriol. 170:4555-4561(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M22623 Genomic DNA. Translation: AAB61150.1.
U57489 Genomic DNA. Translation: AAC45414.1.
PIRA31841.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4IS2X-ray1.90A1-249[»]
4IS3X-ray2.00A/B/C/D1-249[»]
ProteinModelPortalP19337.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:BAIA2EUBSP-MONOMER.
UniPathwayUPA00279.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBAIA2_CLOSV
AccessionPrimary (citable) accession number: P19337
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: January 22, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways