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P19337

- BAIA2_CLOSV

UniProt

P19337 - BAIA2_CLOSV

Protein

Bile acid 7-dehydroxylase 2

Gene

baiA2

Organism
Clostridium scindens (strain JCM 10418 / VPI 12708)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    7-alpha-dehydroxylation of cholic acid and chenodeoxycholate, yielding deoxycholic acid and lithocholic acid, respectively. Highest affinity with taurochenodeoxycholic acid.

    Catalytic activityi

    Deoxycholate + FAD + H2O = cholate + FADH2.
    Lithocholate + FAD + H2O = chenodeoxycholate + FADH2.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei144 – 1441SubstrateBy similarity
    Active sitei157 – 1571Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 137NADSequence Analysis
    Nucleotide bindingi32 – 365NADSequence Analysis

    GO - Molecular functioni

    1. bile-acid 7alpha-dehydroxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. bile acid catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Bile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciMetaCyc:BAIA2EUBSP-MONOMER.
    UniPathwayiUPA00279.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bile acid 7-dehydroxylase 2 (EC:1.17.99.5)
    Alternative name(s):
    Bile acid-inducible protein 2
    Cholate 7-alpha-dehydroxylase 2
    Gene namesi
    Name:baiA2
    OrganismiClostridium scindens (strain JCM 10418 / VPI 12708)
    Taxonomic identifieri29347 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceae

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 249249Bile acid 7-dehydroxylase 2PRO_0000054525Add
    BLAST

    Expressioni

    Inductioni

    Presence of C(24) bile acids containing a 7-alpha-hydroxy group.

    Structurei

    Secondary structure

    1
    249
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni3 – 64
    Beta strandi8 – 114
    Turni12 – 154
    Helixi17 – 2812
    Beta strandi32 – 365
    Helixi40 – 5314
    Beta strandi60 – 623
    Helixi69 – 8315
    Beta strandi88 – 914
    Helixi101 – 1033
    Helixi106 – 11611
    Helixi118 – 13114
    Turni132 – 1354
    Beta strandi137 – 1426
    Helixi146 – 1494
    Helixi160 – 17415
    Helixi176 – 1783
    Beta strandi180 – 1878
    Helixi193 – 1964
    Helixi200 – 20910
    Helixi218 – 22912
    Helixi231 – 2333
    Beta strandi238 – 2436

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4IS2X-ray1.90A1-249[»]
    4IS3X-ray2.00A/B/C/D1-249[»]
    ProteinModelPortaliP19337.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19337-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLVQDKVTI ITGGTRGIGF AAAKIFIDNG AKVSIFGETQ EEVDTALAQL    50
    KELYPEEEVL GFAPDLTSRD AVMAAVGQVA QKYGRLDVMI NNAGITSNNV 100
    FSRVSEEEFK HIMDINVTGV FNGAWCAYQC MKDAKKGVII NTASVTGIFG 150
    SLSGVGYPAS KASVIGLTHG LGREIIRKNI RVVGVAPGVV NTDMTNGNPP 200
    EIMEGYLKAL PMKRMLEPEE IANVYLFLAS DLASGITATT VSVDGAYRP 249
    Length:249
    Mass (Da):26,538
    Last modified:November 1, 1990 - v1
    Checksum:i9A39B78BB63DC5AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22623 Genomic DNA. Translation: AAB61150.1.
    U57489 Genomic DNA. Translation: AAC45414.1.
    PIRiA31841.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22623 Genomic DNA. Translation: AAB61150.1 .
    U57489 Genomic DNA. Translation: AAC45414.1 .
    PIRi A31841.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4IS2 X-ray 1.90 A 1-249 [» ]
    4IS3 X-ray 2.00 A/B/C/D 1-249 [» ]
    ProteinModelPortali P19337.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00279 .
    BioCyci MetaCyc:BAIA2EUBSP-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a bile acid-inducible operon from Eubacterium sp. strain VPI 12708."
      Mallonee D.H., White W.B., Hylemon P.B.
      J. Bacteriol. 172:7011-7019(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Multiple copies of a bile acid-inducible gene in Eubacterium sp. strain VPI 12708."
      Gopal-Srivastava R., Mallonee D.H., White W.B., Hylemon P.B.
      J. Bacteriol. 172:4420-4426(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Evidence for a multigene family involved in bile acid 7-dehydroxylation in Eubacterium sp. strain VPI 12708."
      White W.B., Franklund C.V., Coleman J.P., Hylemon P.B.
      J. Bacteriol. 170:4555-4561(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiBAIA2_CLOSV
    AccessioniPrimary (citable) accession number: P19337
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are three genes for BaiA proteins: baiA1 is identical to baiA3 and there is 81% identity with baiA2.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3