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P19328 (ADA2B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-2B adrenergic receptor
Alternative name(s):
Alpha-2B adrenoreceptor
Short name=Alpha-2B adrenoceptor
Short name=Alpha-2BAR
Gene names
Name:Adra2b
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2B sub-subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processMAPK cascade

Inferred from electronic annotation. Source: Ensembl

activation of MAPK activity

Inferred from mutant phenotype PubMed 11891218. Source: RGD

activation of MAPK activity by adrenergic receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

activation of protein kinase B activity

Inferred from electronic annotation. Source: Ensembl

angiogenesis

Inferred from electronic annotation. Source: Ensembl

epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

female pregnancy

Inferred from mutant phenotype PubMed 19450576. Source: RGD

platelet activation

Inferred from electronic annotation. Source: InterPro

positive regulation of blood pressure

Inferred from mutant phenotype PubMed 17070424. Source: RGD

positive regulation of neuron differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of uterine smooth muscle contraction

Inferred from mutant phenotype PubMed 17664026PubMed 19634638. Source: RGD

regulation of vascular smooth muscle contraction

Inferred from mutant phenotype PubMed 18054907. Source: RGD

   Cellular_componentintegral component of membrane

Traceable author statement PubMed 11891218. Source: RGD

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalpha2-adrenergic receptor activity

Inferred from direct assay Ref.1. Source: RGD

epinephrine binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 453453Alpha-2B adrenergic receptor
PRO_0000069100

Regions

Topological domain1 – 1717Extracellular By similarity
Transmembrane18 – 4225Helical; Name=1; By similarity
Topological domain43 – 5412Cytoplasmic By similarity
Transmembrane55 – 8026Helical; Name=2; By similarity
Topological domain81 – 9010Extracellular By similarity
Transmembrane91 – 11323Helical; Name=3; By similarity
Topological domain114 – 13522Cytoplasmic By similarity
Transmembrane136 – 15823Helical; Name=4; By similarity
Topological domain159 – 17416Extracellular By similarity
Transmembrane175 – 19824Helical; Name=5; By similarity
Topological domain199 – 375177Cytoplasmic By similarity
Transmembrane376 – 39924Helical; Name=6; By similarity
Topological domain400 – 4089Extracellular By similarity
Transmembrane409 – 43224Helical; Name=7; By similarity
Topological domain433 – 45321Cytoplasmic By similarity
Compositional bias300 – 31415Asp/Glu-rich (acidic)

Sites

Site971Implicated in ligand binding By similarity
Site1811Implicated in catechol agonist binding By similarity
Site1851Implicated in catechol agonist binding By similarity

Amino acid modifications

Lipidation4451S-palmitoyl cysteine Potential
Disulfide bond90 ↔ 169 By similarity

Experimental info

Sequence conflict1321R → C in AAA40635. Ref.1
Sequence conflict162 – 1632EP → DA in AAA40635. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P19328 [UniParc].

Last modified October 10, 2003. Version 2.
Checksum: CBA69CE23EACB511

FASTA45350,369
        10         20         30         40         50         60 
MSGPTMDHQE PYSVQATAAI ASAITFLILF TIFGNALVIL AVLTSRSLRA PQNLFLVSLA 

        70         80         90        100        110        120 
AADILVATLI IPFSLANELL GYWYFWRAWC EVYLALDVLF CTSSIVHLCA ISLDRYWAVS 

       130        140        150        160        170        180 
RALEYNSKRT PRRIKCIILT VWLIAAVISL PPLIYKGDQR PEPRGLPQCE LNQEAWYILA 

       190        200        210        220        230        240 
SSIGSFFAPC LIMILVYLRI YVIAKRSHCR GLGAKRGSGE GESKKPQPVA GGVPTSAKVP 

       250        260        270        280        290        300 
TLVSPLSSVG EANGHPKPPR EKEEGETPED PEARALPPTW SALPRSGQGQ KKGTSGATAE 

       310        320        330        340        350        360 
EGDEEDEEEV EECEPQTLPA SPASVCNPPL QQPQTSRVLA TLRGQVLLGK NVGVASGQWW 

       370        380        390        400        410        420 
RRRTQLSREK RFTFVLAVVI GVFVVCWFPF FFSYSLGAIC PQHCKVPHGL FQFFFWIGYC 

       430        440        450 
NSSLNPVIYT VFNQDFRRAF RRILCRPWTQ TGW 

« Hide

References

[1]"Molecular characterization of a rat alpha 2B-adrenergic receptor."
Zeng D., Harrison J.K., D'Angelo D.D., Barber C.M., Tucker A.L., Lu Z., Lynch K.R.
Proc. Natl. Acad. Sci. U.S.A. 87:3102-3106(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
[2]"Cloning and functional characterization of the rat alpha2B-adrenergic receptor gene promoter region: evidence for binding sites for erythropoiesis-related transcription factors GATA1 and NF-E2."
Schaak S., Cussac D., Labialle S., Mignotte V., Paris H.
Biochem. Pharmacol. 70:606-617(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Sprague-Dawley.
[3]"Differential sodium regulation between salt-sensitive and salt-resistant Sabra rats is not due to any mutation in the renal alpha 2B-adrenoceptor gene."
le Jossec M., Cloix J.F., Pecquery R., Giudicelli Y., Dausse J.P.
Am. J. Hypertens. 8:177-182(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-453.
Strain: Sabra.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M32061 mRNA. Translation: AAA40635.1.
AF366899 Genomic DNA. Translation: AAK53388.1.
X74400 Genomic DNA. Translation: CAA52411.1.
PIRA35642.
I51883.
RefSeqNP_612514.2. NM_138505.2.
UniGeneRn.10296.

3D structure databases

ProteinModelPortalP19328.
SMRP19328. Positions 102-133.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP19328.
ChEMBLCHEMBL266.
GuidetoPHARMACOLOGY26.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PaxDbP19328.
PRIDEP19328.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000018584; ENSRNOP00000018584; ENSRNOG00000013887.
GeneID24174.
KEGGrno:24174.
UCSCRGD:2057. rat.

Organism-specific databases

CTD151.
RGD2057. Adra2b.

Phylogenomic databases

eggNOGNOG249628.
GeneTreeENSGT00750000117640.
HOGENOMHOG000239242.
HOVERGENHBG106962.
InParanoidP19328.
KOK04139.
OMAKHCKVPH.
OrthoDBEOG7J9VPT.
PhylomeDBP19328.
TreeFamTF316350.

Gene expression databases

GenevestigatorP19328.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR002233. ADR_fam.
IPR000207. ADRA2B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24248:SF26. PTHR24248:SF26. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01103. ADRENERGICR.
PR00559. ADRENRGCA2BR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio602505.
PROP19328.

Entry information

Entry nameADA2B_RAT
AccessionPrimary (citable) accession number: P19328
Secondary accession number(s): Q63021, Q925E4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 10, 2003
Last modified: June 11, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries