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Protein

Alpha-2B adrenergic receptor

Gene

Adra2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei97 – 971Implicated in ligand bindingBy similarity
Sitei181 – 1811Implicated in catechol agonist bindingBy similarity
Sitei185 – 1851Implicated in catechol agonist bindingBy similarity

GO - Molecular functioni

  1. alpha2-adrenergic receptor activity Source: RGD
  2. epinephrine binding Source: Ensembl

GO - Biological processi

  1. activation of MAPK activity Source: RGD
  2. activation of MAPK activity by adrenergic receptor signaling pathway Source: Ensembl
  3. activation of protein kinase B activity Source: Ensembl
  4. angiogenesis Source: Ensembl
  5. epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway Source: Ensembl
  6. female pregnancy Source: RGD
  7. MAPK cascade Source: Ensembl
  8. platelet activation Source: InterPro
  9. positive regulation of blood pressure Source: RGD
  10. positive regulation of neuron differentiation Source: Ensembl
  11. positive regulation of uterine smooth muscle contraction Source: RGD
  12. regulation of vascular smooth muscle contraction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_284101. Adrenaline signalling through Alpha-2 adrenergic receptor.
REACT_294981. Adrenoceptors.
REACT_339234. G alpha (i) signalling events.
REACT_342580. G alpha (z) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2B adrenergic receptor
Alternative name(s):
Alpha-2B adrenoreceptor
Short name:
Alpha-2B adrenoceptor
Short name:
Alpha-2BAR
Gene namesi
Name:Adra2b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi2057. Adra2b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1717ExtracellularBy similarityAdd
BLAST
Transmembranei18 – 4225Helical; Name=1By similarityAdd
BLAST
Topological domaini43 – 5412CytoplasmicBy similarityAdd
BLAST
Transmembranei55 – 8026Helical; Name=2By similarityAdd
BLAST
Topological domaini81 – 9010ExtracellularBy similarity
Transmembranei91 – 11323Helical; Name=3By similarityAdd
BLAST
Topological domaini114 – 13522CytoplasmicBy similarityAdd
BLAST
Transmembranei136 – 15823Helical; Name=4By similarityAdd
BLAST
Topological domaini159 – 17416ExtracellularBy similarityAdd
BLAST
Transmembranei175 – 19824Helical; Name=5By similarityAdd
BLAST
Topological domaini199 – 375177CytoplasmicBy similarityAdd
BLAST
Transmembranei376 – 39924Helical; Name=6By similarityAdd
BLAST
Topological domaini400 – 4089ExtracellularBy similarity
Transmembranei409 – 43224Helical; Name=7By similarityAdd
BLAST
Topological domaini433 – 45321CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: RGD
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453Alpha-2B adrenergic receptorPRO_0000069100Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi90 ↔ 169PROSITE-ProRule annotation
Lipidationi445 – 4451S-palmitoyl cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP19328.
PRIDEiP19328.

Expressioni

Gene expression databases

GenevestigatoriP19328.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP19328.
SMRiP19328. Positions 102-133.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi300 – 31415Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2B sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP19328.
KOiK04139.
OMAiKHCKVPH.
OrthoDBiEOG7J9VPT.
PhylomeDBiP19328.
TreeFamiTF316350.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR000207. ADRA2B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF26. PTHR24248:SF26. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00559. ADRENRGCA2BR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGPTMDHQE PYSVQATAAI ASAITFLILF TIFGNALVIL AVLTSRSLRA
60 70 80 90 100
PQNLFLVSLA AADILVATLI IPFSLANELL GYWYFWRAWC EVYLALDVLF
110 120 130 140 150
CTSSIVHLCA ISLDRYWAVS RALEYNSKRT PRRIKCIILT VWLIAAVISL
160 170 180 190 200
PPLIYKGDQR PEPRGLPQCE LNQEAWYILA SSIGSFFAPC LIMILVYLRI
210 220 230 240 250
YVIAKRSHCR GLGAKRGSGE GESKKPQPVA GGVPTSAKVP TLVSPLSSVG
260 270 280 290 300
EANGHPKPPR EKEEGETPED PEARALPPTW SALPRSGQGQ KKGTSGATAE
310 320 330 340 350
EGDEEDEEEV EECEPQTLPA SPASVCNPPL QQPQTSRVLA TLRGQVLLGK
360 370 380 390 400
NVGVASGQWW RRRTQLSREK RFTFVLAVVI GVFVVCWFPF FFSYSLGAIC
410 420 430 440 450
PQHCKVPHGL FQFFFWIGYC NSSLNPVIYT VFNQDFRRAF RRILCRPWTQ

TGW
Length:453
Mass (Da):50,369
Last modified:October 9, 2003 - v2
Checksum:iCBA69CE23EACB511
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321R → C in AAA40635 (PubMed:2158103).Curated
Sequence conflicti162 – 1632EP → DA in AAA40635 (PubMed:2158103).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32061 mRNA. Translation: AAA40635.1.
AF366899 Genomic DNA. Translation: AAK53388.1.
X74400 Genomic DNA. Translation: CAA52411.1.
PIRiA35642.
I51883.
RefSeqiNP_612514.2. NM_138505.2.
UniGeneiRn.10296.

Genome annotation databases

EnsembliENSRNOT00000018584; ENSRNOP00000018584; ENSRNOG00000013887.
GeneIDi24174.
KEGGirno:24174.
UCSCiRGD:2057. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32061 mRNA. Translation: AAA40635.1.
AF366899 Genomic DNA. Translation: AAK53388.1.
X74400 Genomic DNA. Translation: CAA52411.1.
PIRiA35642.
I51883.
RefSeqiNP_612514.2. NM_138505.2.
UniGeneiRn.10296.

3D structure databases

ProteinModelPortaliP19328.
SMRiP19328. Positions 102-133.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP19328.
ChEMBLiCHEMBL266.
GuidetoPHARMACOLOGYi26.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiP19328.
PRIDEiP19328.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018584; ENSRNOP00000018584; ENSRNOG00000013887.
GeneIDi24174.
KEGGirno:24174.
UCSCiRGD:2057. rat.

Organism-specific databases

CTDi151.
RGDi2057. Adra2b.

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP19328.
KOiK04139.
OMAiKHCKVPH.
OrthoDBiEOG7J9VPT.
PhylomeDBiP19328.
TreeFamiTF316350.

Enzyme and pathway databases

ReactomeiREACT_284101. Adrenaline signalling through Alpha-2 adrenergic receptor.
REACT_294981. Adrenoceptors.
REACT_339234. G alpha (i) signalling events.
REACT_342580. G alpha (z) signalling events.

Miscellaneous databases

NextBioi602505.
PROiP19328.

Gene expression databases

GenevestigatoriP19328.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR000207. ADRA2B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF26. PTHR24248:SF26. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00559. ADRENRGCA2BR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Cloning and functional characterization of the rat alpha2B-adrenergic receptor gene promoter region: evidence for binding sites for erythropoiesis-related transcription factors GATA1 and NF-E2."
    Schaak S., Cussac D., Labialle S., Mignotte V., Paris H.
    Biochem. Pharmacol. 70:606-617(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Sprague-Dawley.
  3. "Differential sodium regulation between salt-sensitive and salt-resistant Sabra rats is not due to any mutation in the renal alpha 2B-adrenoceptor gene."
    le Jossec M., Cloix J.F., Pecquery R., Giudicelli Y., Dausse J.P.
    Am. J. Hypertens. 8:177-182(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-453.
    Strain: Sabra.
    Tissue: Kidney.

Entry informationi

Entry nameiADA2B_RAT
AccessioniPrimary (citable) accession number: P19328
Secondary accession number(s): Q63021, Q925E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1990
Last sequence update: October 9, 2003
Last modified: March 31, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.