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P19328

- ADA2B_RAT

UniProt

P19328 - ADA2B_RAT

Protein

Alpha-2B adrenergic receptor

Gene

Adra2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (10 Oct 2003)
      Previous versions | rss
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    Functioni

    Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei97 – 971Implicated in ligand bindingBy similarity
    Sitei181 – 1811Implicated in catechol agonist bindingBy similarity
    Sitei185 – 1851Implicated in catechol agonist bindingBy similarity

    GO - Molecular functioni

    1. alpha2-adrenergic receptor activity Source: RGD
    2. epinephrine binding Source: Ensembl

    GO - Biological processi

    1. activation of MAPK activity Source: RGD
    2. activation of MAPK activity by adrenergic receptor signaling pathway Source: Ensembl
    3. activation of protein kinase B activity Source: Ensembl
    4. angiogenesis Source: Ensembl
    5. epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway Source: Ensembl
    6. female pregnancy Source: RGD
    7. MAPK cascade Source: Ensembl
    8. platelet activation Source: InterPro
    9. positive regulation of blood pressure Source: RGD
    10. positive regulation of neuron differentiation Source: Ensembl
    11. positive regulation of uterine smooth muscle contraction Source: RGD
    12. regulation of vascular smooth muscle contraction Source: RGD

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-2B adrenergic receptor
    Alternative name(s):
    Alpha-2B adrenoreceptor
    Short name:
    Alpha-2B adrenoceptor
    Short name:
    Alpha-2BAR
    Gene namesi
    Name:Adra2b
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi2057. Adra2b.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: RGD
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 453453Alpha-2B adrenergic receptorPRO_0000069100Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi90 ↔ 169PROSITE-ProRule annotation
    Lipidationi445 – 4451S-palmitoyl cysteineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP19328.
    PRIDEiP19328.

    Expressioni

    Gene expression databases

    GenevestigatoriP19328.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliP19328.
    SMRiP19328. Positions 102-133.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1717ExtracellularBy similarityAdd
    BLAST
    Topological domaini43 – 5412CytoplasmicBy similarityAdd
    BLAST
    Topological domaini81 – 9010ExtracellularBy similarity
    Topological domaini114 – 13522CytoplasmicBy similarityAdd
    BLAST
    Topological domaini159 – 17416ExtracellularBy similarityAdd
    BLAST
    Topological domaini199 – 375177CytoplasmicBy similarityAdd
    BLAST
    Topological domaini400 – 4089ExtracellularBy similarity
    Topological domaini433 – 45321CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei18 – 4225Helical; Name=1By similarityAdd
    BLAST
    Transmembranei55 – 8026Helical; Name=2By similarityAdd
    BLAST
    Transmembranei91 – 11323Helical; Name=3By similarityAdd
    BLAST
    Transmembranei136 – 15823Helical; Name=4By similarityAdd
    BLAST
    Transmembranei175 – 19824Helical; Name=5By similarityAdd
    BLAST
    Transmembranei376 – 39924Helical; Name=6By similarityAdd
    BLAST
    Transmembranei409 – 43224Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi300 – 31415Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2B sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249628.
    GeneTreeiENSGT00750000117640.
    HOGENOMiHOG000239242.
    HOVERGENiHBG106962.
    InParanoidiP19328.
    KOiK04139.
    OMAiKHCKVPH.
    OrthoDBiEOG7J9VPT.
    PhylomeDBiP19328.
    TreeFamiTF316350.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR002233. ADR_fam.
    IPR000207. ADRA2B_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24248:SF26. PTHR24248:SF26. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR01103. ADRENERGICR.
    PR00559. ADRENRGCA2BR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19328-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGPTMDHQE PYSVQATAAI ASAITFLILF TIFGNALVIL AVLTSRSLRA    50
    PQNLFLVSLA AADILVATLI IPFSLANELL GYWYFWRAWC EVYLALDVLF 100
    CTSSIVHLCA ISLDRYWAVS RALEYNSKRT PRRIKCIILT VWLIAAVISL 150
    PPLIYKGDQR PEPRGLPQCE LNQEAWYILA SSIGSFFAPC LIMILVYLRI 200
    YVIAKRSHCR GLGAKRGSGE GESKKPQPVA GGVPTSAKVP TLVSPLSSVG 250
    EANGHPKPPR EKEEGETPED PEARALPPTW SALPRSGQGQ KKGTSGATAE 300
    EGDEEDEEEV EECEPQTLPA SPASVCNPPL QQPQTSRVLA TLRGQVLLGK 350
    NVGVASGQWW RRRTQLSREK RFTFVLAVVI GVFVVCWFPF FFSYSLGAIC 400
    PQHCKVPHGL FQFFFWIGYC NSSLNPVIYT VFNQDFRRAF RRILCRPWTQ 450
    TGW 453
    Length:453
    Mass (Da):50,369
    Last modified:October 10, 2003 - v2
    Checksum:iCBA69CE23EACB511
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti132 – 1321R → C in AAA40635. (PubMed:2158103)Curated
    Sequence conflicti162 – 1632EP → DA in AAA40635. (PubMed:2158103)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32061 mRNA. Translation: AAA40635.1.
    AF366899 Genomic DNA. Translation: AAK53388.1.
    X74400 Genomic DNA. Translation: CAA52411.1.
    PIRiA35642.
    I51883.
    RefSeqiNP_612514.2. NM_138505.2.
    UniGeneiRn.10296.

    Genome annotation databases

    EnsembliENSRNOT00000018584; ENSRNOP00000018584; ENSRNOG00000013887.
    GeneIDi24174.
    KEGGirno:24174.
    UCSCiRGD:2057. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32061 mRNA. Translation: AAA40635.1 .
    AF366899 Genomic DNA. Translation: AAK53388.1 .
    X74400 Genomic DNA. Translation: CAA52411.1 .
    PIRi A35642.
    I51883.
    RefSeqi NP_612514.2. NM_138505.2.
    UniGenei Rn.10296.

    3D structure databases

    ProteinModelPortali P19328.
    SMRi P19328. Positions 102-133.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P19328.
    ChEMBLi CHEMBL266.
    GuidetoPHARMACOLOGYi 26.

    Protein family/group databases

    GPCRDBi Search...

    Proteomic databases

    PaxDbi P19328.
    PRIDEi P19328.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000018584 ; ENSRNOP00000018584 ; ENSRNOG00000013887 .
    GeneIDi 24174.
    KEGGi rno:24174.
    UCSCi RGD:2057. rat.

    Organism-specific databases

    CTDi 151.
    RGDi 2057. Adra2b.

    Phylogenomic databases

    eggNOGi NOG249628.
    GeneTreei ENSGT00750000117640.
    HOGENOMi HOG000239242.
    HOVERGENi HBG106962.
    InParanoidi P19328.
    KOi K04139.
    OMAi KHCKVPH.
    OrthoDBi EOG7J9VPT.
    PhylomeDBi P19328.
    TreeFami TF316350.

    Miscellaneous databases

    NextBioi 602505.
    PROi P19328.

    Gene expression databases

    Genevestigatori P19328.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR002233. ADR_fam.
    IPR000207. ADRA2B_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24248:SF26. PTHR24248:SF26. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01103. ADRENERGICR.
    PR00559. ADRENRGCA2BR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. "Cloning and functional characterization of the rat alpha2B-adrenergic receptor gene promoter region: evidence for binding sites for erythropoiesis-related transcription factors GATA1 and NF-E2."
      Schaak S., Cussac D., Labialle S., Mignotte V., Paris H.
      Biochem. Pharmacol. 70:606-617(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Sprague-Dawley.
    3. "Differential sodium regulation between salt-sensitive and salt-resistant Sabra rats is not due to any mutation in the renal alpha 2B-adrenoceptor gene."
      le Jossec M., Cloix J.F., Pecquery R., Giudicelli Y., Dausse J.P.
      Am. J. Hypertens. 8:177-182(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-453.
      Strain: Sabra.
      Tissue: Kidney.

    Entry informationi

    Entry nameiADA2B_RAT
    AccessioniPrimary (citable) accession number: P19328
    Secondary accession number(s): Q63021, Q925E4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: October 10, 2003
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3