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Protein

5-hydroxytryptamine receptor 1A

Gene

Htr1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.2 Publications

GO - Molecular functioni

  • drug binding Source: RGD
  • G-protein alpha-subunit binding Source: RGD
  • G-protein coupled serotonin receptor activity Source: RGD
  • neurotransmitter receptor activity Source: GO_Central
  • serotonin binding Source: RGD

GO - Biological processi

  • adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: RGD
  • behavioral fear response Source: UniProtKB
  • cell proliferation Source: InterPro
  • chemical synaptic transmission Source: GO_Central
  • exploration behavior Source: UniProtKB
  • NMDA glutamate receptor clustering Source: RGD
  • positive regulation of microtubule depolymerization Source: RGD
  • regulation of dopamine metabolic process Source: UniProtKB
  • regulation of feeding behavior Source: RGD
  • regulation of hormone secretion Source: InterPro
  • regulation of serotonin secretion Source: UniProtKB
  • serotonin metabolic process Source: UniProtKB
  • serotonin receptor signaling pathway Source: RGD
  • vasoconstriction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 1A
Short name:
5-HT-1A
Short name:
5-HT1A
Alternative name(s):
Serotonin receptor 1A
Gene namesi
Name:Htr1a
Synonyms:5ht1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2845. Htr1a.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36ExtracellularBy similarityAdd BLAST36
Transmembranei37 – 62Helical; Name=1By similarityAdd BLAST26
Topological domaini63 – 73CytoplasmicBy similarityAdd BLAST11
Transmembranei74 – 98Helical; Name=2By similarityAdd BLAST25
Topological domaini99 – 110ExtracellularBy similarityAdd BLAST12
Transmembranei111 – 132Helical; Name=3By similarityAdd BLAST22
Topological domaini133 – 152CytoplasmicBy similarityAdd BLAST20
Transmembranei153 – 178Helical; Name=4By similarityAdd BLAST26
Topological domaini179 – 191ExtracellularBy similarityAdd BLAST13
Transmembranei192 – 217Helical; Name=5By similarityAdd BLAST26
Topological domaini218 – 345CytoplasmicBy similarityAdd BLAST128
Transmembranei346 – 367Helical; Name=6By similarityAdd BLAST22
Topological domaini368 – 378ExtracellularBy similarityAdd BLAST11
Transmembranei379 – 403Helical; Name=7By similarityAdd BLAST25
Topological domaini404 – 422CytoplasmicBy similarityAdd BLAST19

GO - Cellular componenti

  • axon hillock Source: RGD
  • dendrite Source: GO_Central
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: RGD
  • neuronal cell body Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL273.
GuidetoPHARMACOLOGYi1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000689071 – 4225-hydroxytryptamine receptor 1AAdd BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi10N-linked (GlcNAc...)Sequence analysis1
Glycosylationi11N-linked (GlcNAc...)Sequence analysis1
Glycosylationi24N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi109 ↔ 187PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP19327.
PRIDEiP19327.

PTM databases

iPTMnetiP19327.
PhosphoSitePlusiP19327.

Expressioni

Tissue specificityi

Detected in hypothalamus, mesencephalon, amygdala, medulla, thalamus, septum and hippocampus.1 Publication

Interactioni

Subunit structurei

Heterodimer; heterodimerizes with GPER1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Fgfr1Q045896EBI-6570156,EBI-2480918

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: RGD

Protein-protein interaction databases

IntActiP19327. 2 interactors.
STRINGi10116.ENSRNOP00000013618.

Chemistry databases

BindingDBiP19327.

Structurei

3D structure databases

ProteinModelPortaliP19327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni112 – 121Agonist bindingBy similarity10
Regioni358 – 362Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi133 – 135DRY motif; important for ligand-induced conformation changesBy similarity3
Motifi396 – 400NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP19327.
KOiK04153.
PhylomeDBiP19327.

Family and domain databases

InterProiIPR000610. 5HT1A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF20. PTHR24247:SF20. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00512. 5HT1ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVFSFGQGN NTTASQEPFG TGGNVTSISD VTFSYQVITS LLLGTLIFCA
60 70 80 90 100
VLGNACVVAA IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN
110 120 130 140 150
KWTLGQVTCD LFIALDVLCC TSSILHLCAI ALDRYWAITD PIDYVNKRTP
160 170 180 190 200
RRAAALISLT WLIGFLISIP PMLGWRTPED RSDPDACTIS KDHGYTIYST
210 220 230 240 250
FGAFYIPLLL MLVLYGRIFR AARFRIRKTV RKVEKKGAGT SLGTSSAPPP
260 270 280 290 300
KKSLNGQPGS GDWRRCAENR AVGTPCTNGA VRQGDDEATL EVIEVHRVGN
310 320 330 340 350
SKEHLPLPSE SGSNSYAPAC LERKNERNAE AKRKMALARE RKTVKTLGII
360 370 380 390 400
MGTFILCWLP FFIVALVLPF CESSCHMPAL LGAIINWLGY SNSLLNPVIY
410 420
AYFNKDFQNA FKKIIKCKFC RR
Length:422
Mass (Da):46,429
Last modified:November 1, 1990 - v1
Checksum:i19C2731834C4BBC9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti373S → N (PubMed:2167416).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05276 Genomic DNA. Translation: AAA40612.1.
PIRiJH0315.
RefSeqiNP_036717.1. NM_012585.1.
UniGeneiRn.44486.

Genome annotation databases

GeneIDi24473.
KEGGirno:24473.
UCSCiRGD:2845. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05276 Genomic DNA. Translation: AAA40612.1.
PIRiJH0315.
RefSeqiNP_036717.1. NM_012585.1.
UniGeneiRn.44486.

3D structure databases

ProteinModelPortaliP19327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19327. 2 interactors.
STRINGi10116.ENSRNOP00000013618.

Chemistry databases

BindingDBiP19327.
ChEMBLiCHEMBL273.
GuidetoPHARMACOLOGYi1.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP19327.
PhosphoSitePlusiP19327.

Proteomic databases

PaxDbiP19327.
PRIDEiP19327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24473.
KEGGirno:24473.
UCSCiRGD:2845. rat.

Organism-specific databases

CTDi3350.
RGDi2845. Htr1a.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP19327.
KOiK04153.
PhylomeDBiP19327.

Miscellaneous databases

PROiP19327.

Family and domain databases

InterProiIPR000610. 5HT1A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF20. PTHR24247:SF20. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00512. 5HT1ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei5HT1A_RAT
AccessioniPrimary (citable) accession number: P19327
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.