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P19327 (5HT1A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 1A

Short name=5-HT-1A
Short name=5-HT1A
Alternative name(s):
Serotonin receptor 1A
Gene names
Name:Htr1a
Synonyms:5ht1a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli. Ref.1 Ref.3

Subunit structure

Heterodimer; heterodimerizes with GPER1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Detected in hypothalamus, mesencephalon, amygdala, medulla, thalamus, septum and hippocampus. Ref.1

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

Ontologies

Keywords
   Biological processBehavior
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Isopeptide bond
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processN-methyl-D-aspartate receptor clustering

Inferred from direct assay PubMed 18442977. Source: RGD

adenylate cyclase-inhibiting serotonin receptor signaling pathway

Inferred from mutant phenotype PubMed 8271204. Source: RGD

behavioral fear response

Inferred from sequence or structural similarity. Source: UniProtKB

cell proliferation

Inferred from electronic annotation. Source: InterPro

exploration behavior

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of microtubule depolymerization

Inferred from direct assay PubMed 18442977. Source: RGD

regulation of dopamine metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of feeding behavior

Inferred from direct assay PubMed 11900812. Source: RGD

regulation of hormone secretion

Inferred from electronic annotation. Source: InterPro

regulation of serotonin secretion

Inferred from sequence or structural similarity. Source: UniProtKB

serotonin metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

serotonin receptor signaling pathway

Inferred from mutant phenotype PubMed 11927181PubMed 12149253. Source: RGD

vasoconstriction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentaxon hillock

Inferred from direct assay PubMed 18622042. Source: RGD

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

membrane

Inferred from direct assay PubMed 8891585. Source: RGD

neuronal cell body

Inferred from direct assay PubMed 18622042. Source: RGD

   Molecular_functionG-protein alpha-subunit binding

Inferred from physical interaction PubMed 16772521. Source: RGD

drug binding

Inferred from direct assay PubMed 2947981PubMed 8891585. Source: RGD

serotonin binding

Inferred from direct assay PubMed 2947981PubMed 8271204PubMed 8891585. Source: RGD

serotonin receptor activity

Inferred from direct assay PubMed 8271204. Source: RGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Fgfr1Q045896EBI-6570156,EBI-2480918

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4224225-hydroxytryptamine receptor 1A
PRO_0000068907

Regions

Topological domain1 – 3636Extracellular By similarity
Transmembrane37 – 6226Helical; Name=1; By similarity
Topological domain63 – 7311Cytoplasmic By similarity
Transmembrane74 – 9825Helical; Name=2; By similarity
Topological domain99 – 11012Extracellular By similarity
Transmembrane111 – 13222Helical; Name=3; By similarity
Topological domain133 – 15220Cytoplasmic By similarity
Transmembrane153 – 17826Helical; Name=4; By similarity
Topological domain179 – 19113Extracellular By similarity
Transmembrane192 – 21726Helical; Name=5; By similarity
Topological domain218 – 345128Cytoplasmic By similarity
Transmembrane346 – 36722Helical; Name=6; By similarity
Topological domain368 – 37811Extracellular By similarity
Transmembrane379 – 40325Helical; Name=7; By similarity
Topological domain404 – 42219Cytoplasmic By similarity
Region112 – 12110Agonist binding By similarity
Region358 – 3625Agonist binding By similarity
Motif133 – 1353DRY motif; important for ligand-induced conformation changes By similarity
Motif396 – 4005NPxxY motif; important for ligand-induced conformation changes and signaling By similarity

Amino acid modifications

Glycosylation101N-linked (GlcNAc...) Potential
Glycosylation111N-linked (GlcNAc...) Potential
Glycosylation241N-linked (GlcNAc...) Potential
Disulfide bond109 ↔ 187 By similarity
Cross-link334Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict3731S → N Ref.2

Sequences

Sequence LengthMass (Da)Tools
P19327 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 19C2731834C4BBC9

FASTA42246,429
        10         20         30         40         50         60 
MDVFSFGQGN NTTASQEPFG TGGNVTSISD VTFSYQVITS LLLGTLIFCA VLGNACVVAA 

        70         80         90        100        110        120 
IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN KWTLGQVTCD LFIALDVLCC 

       130        140        150        160        170        180 
TSSILHLCAI ALDRYWAITD PIDYVNKRTP RRAAALISLT WLIGFLISIP PMLGWRTPED 

       190        200        210        220        230        240 
RSDPDACTIS KDHGYTIYST FGAFYIPLLL MLVLYGRIFR AARFRIRKTV RKVEKKGAGT 

       250        260        270        280        290        300 
SLGTSSAPPP KKSLNGQPGS GDWRRCAENR AVGTPCTNGA VRQGDDEATL EVIEVHRVGN 

       310        320        330        340        350        360 
SKEHLPLPSE SGSNSYAPAC LERKNERNAE AKRKMALARE RKTVKTLGII MGTFILCWLP 

       370        380        390        400        410        420 
FFIVALVLPF CESSCHMPAL LGAIINWLGY SNSLLNPVIY AYFNKDFQNA FKKIIKCKFC 


RR 

« Hide

References

[1]"Cloning, functional expression, and mRNA tissue distribution of the rat 5-hydroxytryptamine1A receptor gene."
Albert P.R., Zhou Q.-Y., van Tol H.H.M., Bunzow J.R., Civelli O.
J. Biol. Chem. 265:5825-5832(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"Role of cytochrome P450 in the control of the production of erythropoietin."
Fujiwara Y., Nelson D.L., Kashihara K., Varga E., Roeske W.R., Yamamura H.I.
Life Sci. 47:127-134(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Serotonin receptor activation reduces calcium current in an acutely dissociated adult central neuron."
Penington N.J., Kelly J.S.
Neuron 4:751-758(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05276 Genomic DNA. Translation: AAA40612.1.
PIRJH0315.
RefSeqNP_036717.1. NM_012585.1.
UniGeneRn.44486.

3D structure databases

ProteinModelPortalP19327.
SMRP19327. Positions 121-152.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP19327. 2 interactions.
STRING10116.ENSRNOP00000013618.

Chemistry

BindingDBP19327.
ChEMBLCHEMBL2095159.
GuidetoPHARMACOLOGY1.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP19327.

Proteomic databases

PaxDbP19327.
PRIDEP19327.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24473.
KEGGrno:24473.
UCSCRGD:2845. rat.

Organism-specific databases

CTD3350.
RGD2845. Htr1a.

Phylogenomic databases

eggNOGNOG249628.
HOGENOMHOG000239242.
HOVERGENHBG106962.
InParanoidP19327.
KOK04153.
PhylomeDBP19327.

Gene expression databases

GenevestigatorP19327.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000610. 5HT1A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24247:SF20. PTHR24247:SF20. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00512. 5HT1ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603421.
PROP19327.

Entry information

Entry name5HT1A_RAT
AccessionPrimary (citable) accession number: P19327
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries