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P19327

- 5HT1A_RAT

UniProt

P19327 - 5HT1A_RAT

Protein

5-hydroxytryptamine receptor 1A

Gene

Htr1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.2 Publications

    GO - Molecular functioni

    1. drug binding Source: RGD
    2. G-protein alpha-subunit binding Source: RGD
    3. protein binding Source: IntAct
    4. serotonin binding Source: RGD
    5. serotonin receptor activity Source: RGD

    GO - Biological processi

    1. adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: RGD
    2. behavioral fear response Source: UniProtKB
    3. cell proliferation Source: InterPro
    4. exploration behavior Source: UniProtKB
    5. N-methyl-D-aspartate receptor clustering Source: RGD
    6. positive regulation of microtubule depolymerization Source: RGD
    7. regulation of dopamine metabolic process Source: UniProtKB
    8. regulation of feeding behavior Source: RGD
    9. regulation of hormone secretion Source: InterPro
    10. regulation of serotonin secretion Source: UniProtKB
    11. serotonin metabolic process Source: UniProtKB
    12. serotonin receptor signaling pathway Source: RGD
    13. vasoconstriction Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Behavior

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-hydroxytryptamine receptor 1A
    Short name:
    5-HT-1A
    Short name:
    5-HT1A
    Alternative name(s):
    Serotonin receptor 1A
    Gene namesi
    Name:Htr1a
    Synonyms:5ht1a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2845. Htr1a.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. axon hillock Source: RGD
    2. integral component of plasma membrane Source: UniProtKB
    3. membrane Source: RGD
    4. neuronal cell body Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4224225-hydroxytryptamine receptor 1APRO_0000068907Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi10 – 101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi11 – 111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi109 ↔ 187PROSITE-ProRule annotation
    Cross-linki334 – 334Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiP19327.
    PRIDEiP19327.

    PTM databases

    PhosphoSiteiP19327.

    Expressioni

    Tissue specificityi

    Detected in hypothalamus, mesencephalon, amygdala, medulla, thalamus, septum and hippocampus.1 Publication

    Gene expression databases

    GenevestigatoriP19327.

    Interactioni

    Subunit structurei

    Heterodimer; heterodimerizes with GPER1.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Fgfr1Q045896EBI-6570156,EBI-2480918

    Protein-protein interaction databases

    IntActiP19327. 2 interactions.
    STRINGi10116.ENSRNOP00000013618.

    Structurei

    3D structure databases

    ProteinModelPortaliP19327.
    SMRiP19327. Positions 121-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3636ExtracellularBy similarityAdd
    BLAST
    Topological domaini63 – 7311CytoplasmicBy similarityAdd
    BLAST
    Topological domaini99 – 11012ExtracellularBy similarityAdd
    BLAST
    Topological domaini133 – 15220CytoplasmicBy similarityAdd
    BLAST
    Topological domaini179 – 19113ExtracellularBy similarityAdd
    BLAST
    Topological domaini218 – 345128CytoplasmicBy similarityAdd
    BLAST
    Topological domaini368 – 37811ExtracellularBy similarityAdd
    BLAST
    Topological domaini404 – 42219CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei37 – 6226Helical; Name=1By similarityAdd
    BLAST
    Transmembranei74 – 9825Helical; Name=2By similarityAdd
    BLAST
    Transmembranei111 – 13222Helical; Name=3By similarityAdd
    BLAST
    Transmembranei153 – 17826Helical; Name=4By similarityAdd
    BLAST
    Transmembranei192 – 21726Helical; Name=5By similarityAdd
    BLAST
    Transmembranei346 – 36722Helical; Name=6By similarityAdd
    BLAST
    Transmembranei379 – 40325Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni112 – 12110Agonist bindingBy similarity
    Regioni358 – 3625Agonist bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi133 – 1353DRY motif; important for ligand-induced conformation changesBy similarity
    Motifi396 – 4005NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249628.
    HOGENOMiHOG000239242.
    HOVERGENiHBG106962.
    InParanoidiP19327.
    KOiK04153.
    PhylomeDBiP19327.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000610. 5HT1A_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24247:SF20. PTHR24247:SF20. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00512. 5HT1ARECEPTR.
    PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19327-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVFSFGQGN NTTASQEPFG TGGNVTSISD VTFSYQVITS LLLGTLIFCA    50
    VLGNACVVAA IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN 100
    KWTLGQVTCD LFIALDVLCC TSSILHLCAI ALDRYWAITD PIDYVNKRTP 150
    RRAAALISLT WLIGFLISIP PMLGWRTPED RSDPDACTIS KDHGYTIYST 200
    FGAFYIPLLL MLVLYGRIFR AARFRIRKTV RKVEKKGAGT SLGTSSAPPP 250
    KKSLNGQPGS GDWRRCAENR AVGTPCTNGA VRQGDDEATL EVIEVHRVGN 300
    SKEHLPLPSE SGSNSYAPAC LERKNERNAE AKRKMALARE RKTVKTLGII 350
    MGTFILCWLP FFIVALVLPF CESSCHMPAL LGAIINWLGY SNSLLNPVIY 400
    AYFNKDFQNA FKKIIKCKFC RR 422
    Length:422
    Mass (Da):46,429
    Last modified:November 1, 1990 - v1
    Checksum:i19C2731834C4BBC9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti373 – 3731S → N(PubMed:2167416)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05276 Genomic DNA. Translation: AAA40612.1.
    PIRiJH0315.
    RefSeqiNP_036717.1. NM_012585.1.
    UniGeneiRn.44486.

    Genome annotation databases

    GeneIDi24473.
    KEGGirno:24473.
    UCSCiRGD:2845. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05276 Genomic DNA. Translation: AAA40612.1 .
    PIRi JH0315.
    RefSeqi NP_036717.1. NM_012585.1.
    UniGenei Rn.44486.

    3D structure databases

    ProteinModelPortali P19327.
    SMRi P19327. Positions 121-152.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P19327. 2 interactions.
    STRINGi 10116.ENSRNOP00000013618.

    Chemistry

    BindingDBi P19327.
    ChEMBLi CHEMBL2095159.
    GuidetoPHARMACOLOGYi 1.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P19327.

    Proteomic databases

    PaxDbi P19327.
    PRIDEi P19327.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24473.
    KEGGi rno:24473.
    UCSCi RGD:2845. rat.

    Organism-specific databases

    CTDi 3350.
    RGDi 2845. Htr1a.

    Phylogenomic databases

    eggNOGi NOG249628.
    HOGENOMi HOG000239242.
    HOVERGENi HBG106962.
    InParanoidi P19327.
    KOi K04153.
    PhylomeDBi P19327.

    Miscellaneous databases

    NextBioi 603421.
    PROi P19327.

    Gene expression databases

    Genevestigatori P19327.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000610. 5HT1A_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24247:SF20. PTHR24247:SF20. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00512. 5HT1ARECEPTR.
    PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, functional expression, and mRNA tissue distribution of the rat 5-hydroxytryptamine1A receptor gene."
      Albert P.R., Zhou Q.-Y., van Tol H.H.M., Bunzow J.R., Civelli O.
      J. Biol. Chem. 265:5825-5832(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "Role of cytochrome P450 in the control of the production of erythropoietin."
      Fujiwara Y., Nelson D.L., Kashihara K., Varga E., Roeske W.R., Yamamura H.I.
      Life Sci. 47:127-134(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Serotonin receptor activation reduces calcium current in an acutely dissociated adult central neuron."
      Penington N.J., Kelly J.S.
      Neuron 4:751-758(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry namei5HT1A_RAT
    AccessioniPrimary (citable) accession number: P19327
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3