Reviewed, UniProtKB/Swiss-Prot P19323 (FHLA_ECOLI)

Names and origin

Protein names

Recommended name:
    Formate hydrogenlyase transcriptional activator

Gene names

Name:	fhlA
Ordered Locus Names:	b2731, JW2701

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes.
Sequence similarities	Contains 1 GAF domain. Contains 1 sigma-54 factor interaction domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Activator
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro transcription factor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-1113147,EBI-543750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 692	692	Formate hydrogenlyase transcriptional activator		PRO_0000081297
Regions
	Domain	202 – 344	143	GAF
	Domain	381 – 610	230	Sigma-54 factor interaction
	Nucleotide binding	409 – 416	8	ATP Potential
	Nucleotide binding	472 – 481	10	ATP Potential
	DNA binding	663 – 682	20	H-T-H motif By similarity
Experimental info
	Sequence conflict	39 – 49	11	KRSALADNAAI → NVLRRQRGY in AAA23770. Ref.2
	Sequence conflict	59 – 71	13	ASYYA…KDTPI → RLISVACKRHPH Ref.2
	Sequence conflict	210	1	V → L in AAA23770. Ref.2
	Sequence conflict	284 – 286	3	PYE → L Ref.2
	Sequence conflict	289 – 333	45	LFDTW…NLNLL → SSTPGATDSNLVPVTADVWR HHAGRAETGANRRESVYHYQ SEFT Ref.2
	Sequence conflict	666	1	A → R in AAA23770. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P19323-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: EB43989C92DFBA5A	FASTA	692	78,468
	10 20 30 40 50 60 MSYTPMSDLG QQGLFDITRT LLQQPDLASL CEALSQLVKR SALADNAAIV LWQAQTQRAS 70 80 90 100 110 120 YYASREKDTP IKYEDETVLA HGPVRSILSR PDTLHCSYEE FCETWPQLDA GGLYPKFGHY 130 140 150 160 170 180 CLMPLAAEGH IFGGCEFIRY DDRPWSEKEF NRLQTFTQIV SVVTEQIQSR VVNNVDYELL 190 200 210 220 230 240 CRERDNFRIL VAITNAVLSR LDMDELVSEV AKEIHYYFDI DDISIVLRSH RKNKLNIYST 250 260 270 280 290 300 HYLDKQHPAH EQSEVDEAGT LTERVFKSKE MLLINLHERD DLAPYERMLF DTWGNQIQTL 310 320 330 340 350 360 CLLPLMSGDT MLGVLKLAQC EEKVFTTTNL NLLRQIAERV AIAVDNALAY QEIHRLKERL 370 380 390 400 410 420 VDENLALTEQ LNNVDSEFGE IIGRSEAMYS VLKQVEMVAQ SDSTVLILGE TGTGKELIAR 430 440 450 460 470 480 AIHNLSGRNN RRMVKMNCAA MPAGLLESDL FGHERGAFTG ASAQRIGRFE LADKSSLFLD 490 500 510 520 530 540 EVGDMPLELQ PKLLRVLQEQ EFERLGSNKI IQTDVRLIAA TNRDLKKMVA DREFRSDLYY 550 560 570 580 590 600 RLNVFPIHLP PLRERPEDIP LLAKAFTFKI ARRLGRNIDS IPAETLRTLS NMEWPGNVRE 610 620 630 640 650 660 LENVIERAVL LTRGNVLQLS LPDIVLPEPE TPPAATVVAL EGEDEYQLIV RVLKETNGVV 670 680 690 AGPKGAAQRL GLKRTTLLSR MKRLGIDKSA LI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and sequence analysis of the gene encoding the transcriptional activator of the formate hydrogenlyase system of Escherichia coli." Schlensog V., Boeck A. Mol. Microbiol. 4:1319-1327(1990) [PubMed: 2280686] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[2]	"Genetic regulation of formate hydrogenlyase of Escherichia coli: role of the fhlA gene product as a transcriptional activator for a new regulatory gene, fhlB." Maupin J.A., Shanmugam K.T. J. Bacteriol. 172:4798-4806(1990) [PubMed: 2118503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52227 Genomic DNA. Translation: CAA36476.1. U29579 Genomic DNA. Translation: AAA69241.1. M58504 Genomic DNA. Translation: AAA23770.1. U00096 Genomic DNA. Translation: AAC75773.1. AP009048 Genomic DNA. Translation: BAE76808.1.
PIR	S12079.
RefSeq	AP_003298.1. NP_417211.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P19323. 3 interactions.
Genome annotation databases
GeneID	947181.
GenomeReviews	Gene locus JW2701 in contig AP009048_GR. Gene locus b2731 in contig U00096_GR.
KEGG	ecj:JW2701. eco:b2731.
Organism-specific databases
EchoBASE	EB0297.
EcoGene	EG10301. fhlA.
CMR	Search...
Phylogenomic databases
HOGENOM	P19323.
OMA	P19323. GHYCLLP.
Enzyme and pathway databases
BioCyc	EcoCyc:PD02936.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003018. GAF. IPR002197. HTH_Fis. IPR002078. RNA_pol_sigma_54_int. [Graphical view]
Pfam	PF01590. GAF. 1 hit. PF02954. HTH_8. 1 hit. PF00158. Sigma54_activat. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00065. GAF. 2 hits. [Graphical view]
PROSITE	PS00675. SIGMA54_INTERACT_1. 1 hit. PS00676. SIGMA54_INTERACT_2. False negative. PS00688. SIGMA54_INTERACT_3. 1 hit. PS50045. SIGMA54_INTERACT_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FHLA_ECOLI

Accession

Primary (citable) accession number: P19323
Secondary accession number(s): Q2MA98, Q47214

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)