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Protein

Formate hydrogenlyase transcriptional activator

Gene

fhlA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi409 – 4168ATPPROSITE-ProRule annotation
Nucleotide bindingi472 – 48110ATPPROSITE-ProRule annotation
DNA bindingi663 – 68220H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD02936.
ECOL316407:JW2701-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate hydrogenlyase transcriptional activator
Gene namesi
Name:fhlA
Ordered Locus Names:b2731, JW2701
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10301. fhlA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692Formate hydrogenlyase transcriptional activatorPRO_0000081297Add
BLAST

Proteomic databases

PaxDbiP19323.
PRIDEiP19323.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F52EBI-1113147,EBI-543750

Protein-protein interaction databases

BioGridi4261423. 14 interactions.
IntActiP19323. 4 interactions.
STRINGi511145.b2731.

Structurei

3D structure databases

ProteinModelPortaliP19323.
SMRiP19323. Positions 338-683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 344143GAFAdd
BLAST
Domaini381 – 610230Sigma-54 factor interactionPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GAF domain.Curated
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108JUA. Bacteria.
COG3604. LUCA.
HOGENOMiHOG000058488.
InParanoidiP19323.
KOiK15836.
OMAiNTMLGVL.
OrthoDBiEOG6WHNMG.
PhylomeDBiP19323.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 2 hits.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19323-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYTPMSDLG QQGLFDITRT LLQQPDLASL CEALSQLVKR SALADNAAIV
60 70 80 90 100
LWQAQTQRAS YYASREKDTP IKYEDETVLA HGPVRSILSR PDTLHCSYEE
110 120 130 140 150
FCETWPQLDA GGLYPKFGHY CLMPLAAEGH IFGGCEFIRY DDRPWSEKEF
160 170 180 190 200
NRLQTFTQIV SVVTEQIQSR VVNNVDYELL CRERDNFRIL VAITNAVLSR
210 220 230 240 250
LDMDELVSEV AKEIHYYFDI DDISIVLRSH RKNKLNIYST HYLDKQHPAH
260 270 280 290 300
EQSEVDEAGT LTERVFKSKE MLLINLHERD DLAPYERMLF DTWGNQIQTL
310 320 330 340 350
CLLPLMSGDT MLGVLKLAQC EEKVFTTTNL NLLRQIAERV AIAVDNALAY
360 370 380 390 400
QEIHRLKERL VDENLALTEQ LNNVDSEFGE IIGRSEAMYS VLKQVEMVAQ
410 420 430 440 450
SDSTVLILGE TGTGKELIAR AIHNLSGRNN RRMVKMNCAA MPAGLLESDL
460 470 480 490 500
FGHERGAFTG ASAQRIGRFE LADKSSLFLD EVGDMPLELQ PKLLRVLQEQ
510 520 530 540 550
EFERLGSNKI IQTDVRLIAA TNRDLKKMVA DREFRSDLYY RLNVFPIHLP
560 570 580 590 600
PLRERPEDIP LLAKAFTFKI ARRLGRNIDS IPAETLRTLS NMEWPGNVRE
610 620 630 640 650
LENVIERAVL LTRGNVLQLS LPDIVLPEPE TPPAATVVAL EGEDEYQLIV
660 670 680 690
RVLKETNGVV AGPKGAAQRL GLKRTTLLSR MKRLGIDKSA LI
Length:692
Mass (Da):78,468
Last modified:November 1, 1990 - v1
Checksum:iEB43989C92DFBA5A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 4911KRSALADNAAI → NVLRRQRGY in AAA23770 (PubMed:2118503).CuratedAdd
BLAST
Sequence conflicti59 – 7113ASYYA…KDTPI → RLISVACKRHPH (PubMed:2118503).CuratedAdd
BLAST
Sequence conflicti210 – 2101V → L in AAA23770 (PubMed:2118503).Curated
Sequence conflicti284 – 2863PYE → L (PubMed:2118503).Curated
Sequence conflicti289 – 33345LFDTW…NLNLL → SSTPGATDSNLVPVTADVWR HHAGRAETGANRRESVYHYQ SEFT (PubMed:2118503).CuratedAdd
BLAST
Sequence conflicti666 – 6661A → R in AAA23770 (PubMed:2118503).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52227 Genomic DNA. Translation: CAA36476.1.
U29579 Genomic DNA. Translation: AAA69241.1.
M58504 Genomic DNA. Translation: AAA23770.1.
U00096 Genomic DNA. Translation: AAC75773.1.
AP009048 Genomic DNA. Translation: BAE76808.1.
PIRiS12079.
RefSeqiNP_417211.1. NC_000913.3.
WP_001301332.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75773; AAC75773; b2731.
BAE76808; BAE76808; BAE76808.
GeneIDi947181.
KEGGiecj:JW2701.
eco:b2731.
PATRICi32120862. VBIEscCol129921_2823.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52227 Genomic DNA. Translation: CAA36476.1.
U29579 Genomic DNA. Translation: AAA69241.1.
M58504 Genomic DNA. Translation: AAA23770.1.
U00096 Genomic DNA. Translation: AAC75773.1.
AP009048 Genomic DNA. Translation: BAE76808.1.
PIRiS12079.
RefSeqiNP_417211.1. NC_000913.3.
WP_001301332.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP19323.
SMRiP19323. Positions 338-683.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261423. 14 interactions.
IntActiP19323. 4 interactions.
STRINGi511145.b2731.

Proteomic databases

PaxDbiP19323.
PRIDEiP19323.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75773; AAC75773; b2731.
BAE76808; BAE76808; BAE76808.
GeneIDi947181.
KEGGiecj:JW2701.
eco:b2731.
PATRICi32120862. VBIEscCol129921_2823.

Organism-specific databases

EchoBASEiEB0297.
EcoGeneiEG10301. fhlA.

Phylogenomic databases

eggNOGiENOG4108JUA. Bacteria.
COG3604. LUCA.
HOGENOMiHOG000058488.
InParanoidiP19323.
KOiK15836.
OMAiNTMLGVL.
OrthoDBiEOG6WHNMG.
PhylomeDBiP19323.

Enzyme and pathway databases

BioCyciEcoCyc:PD02936.
ECOL316407:JW2701-MONOMER.

Miscellaneous databases

PROiP19323.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 2 hits.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and sequence analysis of the gene encoding the transcriptional activator of the formate hydrogenlyase system of Escherichia coli."
    Schlensog V., Boeck A.
    Mol. Microbiol. 4:1319-1327(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. "Genetic regulation of formate hydrogenlyase of Escherichia coli: role of the fhlA gene product as a transcriptional activator for a new regulatory gene, fhlB."
    Maupin J.A., Shanmugam K.T.
    J. Bacteriol. 172:4798-4806(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiFHLA_ECOLI
AccessioniPrimary (citable) accession number: P19323
Secondary accession number(s): Q2MA98, Q47214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: February 17, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.